ライフサイエンスコーパス: PKC-delta

1 ase C (PKC), specifically the delta-isoform (PKC delta).

2 ly phosphorylated by protein kinase C delta (PKC delta).

3 s that are marked by protein kinase C-delta (PKC-delta).

4 (CEl), which express protein kinase C-delta (PKC-delta).

5 e cancer cells through a mechanism involving PKC delta.

6 r translocation precedes caspase cleavage of PKC delta.

7 27) in their binding with the C1b domain of PKC delta.

8 sis by blocking nuclear import of endogenous PKC delta.

9 tyrosine phosphorylation of c-Src kinase and PKC delta.

10 uced up-regulation of p53 by down-regulating PKC delta.

11 reversed by the forced expression of PCAF or PKC delta.

12 induced by a constitutively active mutant of PKC-delta.

13 by rottlerin (10 muM), suggesting a role for PKC-delta.

14 ate the mechanism of PLS3 phosphorylation by PKC-delta.

15 a regulatory kinase for NKCC1, with PP2A and PKC-delta.

16 1 position is disfavored by PKC-zeta but not PKC-delta.

17 T29A/S97A, by an in vitro kinase assay with PKC-delta.

18 poptotic machinery through the activation of PKC-delta.

19 ly blocked by the inhibition of PKC-beta and PKC-delta.

20 r-induced conformational change that renders PKC delta a better substrate for phosphorylation by prec

21 poptosis and suppress tumorigenicity, making PKC-delta a potential tumor suppressor gene for SCCs.

22 ed downregulation of protein kinase C-delta (PKC-delta), a TLR4-associated signalling mediator requir

23 mechanism involving protein kinase C delta (PKC delta): (a) PCPH knockdown in LNCaP cells decreased

24 cytes, together with decreased activation of PKC-delta, a caspase 3 substrate which functions as a po

25 Here, we report that PKC-delta, a novel PKC isoform, plays a role in EGF-depe

26 to TGF-beta stimulation, and the blockade of PKC delta abrogated both the phosphorylation and acetyla

27 on as an additional mechanism that regulates PKC delta actions in cardiomyocytes.

28 o use FTY720 as a scaffold to develop potent PKC delta-activating agents for HCC therapy.

29 tor protected Huh7 cells from FTY720-induced PKC delta activation and caspase-3-dependent apoptosis.

30 stimulates tumor development mainly through PKC-delta- activation of p62.

31 orylation were significantly enhanced by the PKC delta activator bryostatin 1.

32 ne N-benzyladriamycin-14-valerate (AD198), a PKC-delta activator, to investigate the mechanism of PLS

33 Decreasing PKC-delta activity (pharmacological, dominant-negative,

34 We observed increased PKC-delta activity and an increase in the number of acel

35 icidal activity by LTs was also dependent on PKC-delta activity.

36 a; TGF-beta stimulates, while IL-7 inhibits, PKC-delta activity.

37 manner dependent on protein kinase C-delta (PKC-delta) activity.

38 nsfection of a wild-type construct increased PKC delta and enhanced mucin secretion and MARCKS phosph

39 immunoblot analysis with antibodies to total PKC delta and PKC delta-pY(311), we demonstrate that PKC

40 Particularly, differentiation-promoting PKC delta and PKC eta elicit Pyk2 activation.

41 rmore, we showed that shear stress activates PKC delta and that the PKC delta peptide antagonist, del

42 We identified PKC delta and varepsilon as required and sufficient to a

43 PKC-delta and -zeta predictions have been validated rigo

44 A novel assay in which the kinase domain of PKC-delta and its substrate (a fusion protein of PKC sub

45 OAG prevented the ability of OAG to activate PKC-delta and lower glucose production.

46 o drug treatment relies on the activation of PKC-delta and NF-kappaB-activating kinase (NAK), indepen

47 Furthermore, PKC-delta and nSMase2 did not coimmunoprecipitate, sugge

48 Thus, these data reveal an important role of PKC-delta and PI3K/Akt pathways in activating mTOR as an

49 not apoptotic to the cells, suggesting that PKC-delta and PKC-alpha/beta function oppositely to faci

50 e recovery process is mediated by both novel PKC-delta and PKC-epsilon isozymes and by conventional P

51 also induced the association between HuR and PKC-delta and promoted the phosphorylation of HuR.

52 nclude that Lyn-mediated phosphorylations of PKC-delta and SHIP-1 and their associations negatively r

53 ation promotes physical interactions between PKC-delta and Sp1 resulting in phosphorylation and nucle

54 Rho protein inhibitor), suggesting that the PKC-delta and the Rho/ROK pathways are necessary for MAR

55 his pathway required protein kinase C-delta (PKC-delta) and the guanine nucleotide-exchange factor Ra

56 tated with NKCC1 and protein kinase C-delta (PKC-delta) and was pulled down by a recombinant N termin

57 n of PKC-delta, but not the kinase-defective PKC-delta, and AD198 treatment enhanced threonine phosph

58 Additional studies revealed that PKC-alpha, PKC-delta, and p47Phox knockdown significantly abrogated

59 ol-to-membrane translocation of PKC-epsilon, PKC-delta, and PKA-alpha, -gamma, and -IIalphareg in Eol

60 RasGRP3 activation occurs via PKC delta- and varepsilon-dependent phosphorylation and

61 and its downstream target p70 S6 kinase in a PKC-delta- and PI3K/Akt-dependent manner.

62 erent PKC isoforms (PKC-alpha, -beta1/2, and PKC-delta) are linked to the development of pathologies

63 ults also implicate targeted interruption of PKC-delta as a potential therapeutic option in asbestos-

64 Taken together, these data implicate PKC-delta as a regulator of nSMase2 and, for the first t

65 mmunoprecipitation studies demonstrated that PKC delta associated with retinoic acid receptor-alpha a

66 The activated PKC-delta associates with and phosphorylates p73 to init

67 tes, which have selective down-regulation of PKC-delta at both protein and mRNA levels, had significa

68 on (at the C-terminal hydrophobic motif) and PKC delta (at the activation loop) as events that accomp

69 studies demonstrated that upon TCR ligation, PKC delta becomes rapidly phosphorylated on the activati

70 Inhibiting PKC delta blocked both basal transcription of the human

71 Moreover, a dominant-negative mutant of PKC-delta blocked albumin-induced apoptosis in RPTC cell

72 ibitor lapatinib, as well as by knockdown of PKC-delta but not that of PKC-alpha.

73 Overexpression of PKC-delta, but not the kinase-defective PKC-delta, and A

74 983 and bisindolylmaleimide, or depletion of PKC delta by siRNA had no effect on the down-regulation

75 that p38-MAPK is required for activation of PKC-delta by EGF while inhibition of PKC-delta had no di

76 Inhibition of PKC-delta by pharmacological and genetic approaches prev

77 Re-expression of PKC-delta by retrovirus transduction caused an increase

78 Inhibition of PKC-delta by rottlerin (a relatively specific inhibitor

79 n a 16HBE14o- cell line inducibly expressing PKC delta-CAT under the tet-off system.

80 ively active catalytic subunit of PKC delta (PKC delta-CAT), and treatment with bryostatin 1, an acti

81 e studies demonstrate that generation of the PKC delta catalytic fragment is a critical step for comm

82 nt with a G1 arrest, increased expression of PKC-delta caused rapid and significant downregulation of

83 esults from Src activation and increased Src-PKC delta complex formation.

84 gh siRNA or dominant-negative expression) of PKC-delta confirm a role for this PKC isoform in EGF-dep

85 A dominant-negative PKC delta construct (pEGFP-N1/PKC delta K376R) transfect

86 tional and novel PKC isozymes and that novel PKC-delta contributes specifically to the maintenance of

87 The growth inhibition induced by PKC-delta could be partially reversed by Bcl-x(L) expres

88 LR2 by 61% (P < 0.05), whereas inhibition of PKC-delta decreased TLR4 under high glucose by 63% (P <

89 This checkpoint was impaired in PKC-delta-deficient mice, which developed B cell autoimm

90 mmatory DC responses and indeed, blocking of PKC-delta degradation by the autophagolysosomal inhibito

91 In this study, we further characterized the PKC-delta dependent signaling pathways involved in these

92 enhanceAM microbicidal activity through the PKC-delta-dependent activation of NADPH oxidase.

93 Thus, PMA stimulates a PKC-delta-dependent, TORC2-independent signaling cascade

94 d not increase in vitro N-SMase activity and PKC-delta did not regulate TNF-induced N-SMase activity.

95 sosomes in CD8(+) CTL and demonstrating that PKC delta directly transduces TCR signals leading to pol

96 ly, we show that kinase negative full-length PKC delta does not translocate to the nucleus in apoptot

97 mulated p38 MAPK activation, indicating that PKC-delta does not act through p38 MAPK in regulating nS

98 Unlike other protein kinase Cs (PKC), PKC-delta does not require phosphorylation of its activa

99 grossly alter the kinetics of PMA-dependent PKC delta down-regulation.

100 e of 90% in the SCCs tested, consistent with PKC-delta down-regulation at the protein level.

101 e of MARCKS in NT secretion was regulated by PKC-delta downstream of the Rho/ROK pathway.

102 treatment with bryostatin 1, an activator of PKC delta, each increased transcription from the IL-8 pr

103 e observed phosphorylation and activation of PKC-delta early during treatment of RPTC cells with albu

104 rsistently activates protein kinase C-delta (PKC-delta, encoded by Prkcd) and p38alpha mitogen-activa

105 Overexpression of a functional PKC-delta enhanced COX-2 expression indicating that PKC-

106 Here we show that PKC-delta-expressing central amygdala neurons are essent

107 To better understand how PKC delta facilitates granule movement, here we studied

108 tool to probe the role of the C1b domain in PKC delta function, where the response to the DAG-lacton

109 r this unusual capacity and its relevance to PKC-delta function in intact cells.

110 Together, these results indicate that PKC-delta gene expression is suppressed in human SCCs, p

111 A from nine of the same tumors revealed that PKC-delta gene was deleted in only one tumor.

112 all cells, whereas the catalytic fragment of PKC delta, generated by caspase cleavage, is only presen

113 were analyzed, and the result revealed that PKC-delta genes comprise 12, 18, 19, and 18 exons for C.

114 tion of PKC-delta by EGF while inhibition of PKC-delta had no discernible effects on p38-MAPK activat

115 , whereas rottlerin, a specific inhibitor of PKC delta, had no effect.

116 These data show that modulation of PKC-delta has multiple effects on peribronchiolar cell p

117 by the novel protein kinase C (PKC) isoform PKC delta in 16HBE14o- human airway epithelial cells, fo

118 PCPH knockdown; and (c) forced expression of PKC delta in cells with knocked down PCPH reverted all c

119 ed dynamics of intracellular localization of PKC delta in living CD8(+) CTL.

120 Here, we further investigated the role of PKC delta in mucin hypersecretion using both primary hum

121 at PLS3 is a critical downstream effector of PKC-delta in AD198-induced apoptosis.

122 e an important tumor suppressor function for PKC-delta in colonic carcinogenesis.

123 Furthermore, overexpression of PKC-delta in human SCC lines and mouse epidermis is suff

124 PKC-delta in particular showed a different pattern of tr

125 ntial regulation of dense granule release by PKC-delta in platelets.

126 In vivo, we observed activated PKC-delta in proteinuric kidneys of streptozotocin-induc

127 Overexpressing active PKC-delta in Tam-sensitive MCF-7 cells (PKC-delta/MCF-7)

128 ta-/-) mice to demonstrate multiple roles of PKC-delta in the development of cell proliferation and i

129 We determined the role of PKC-delta in the regulation of pulmonary fibroblast coll

130 These results place Syk downstream of PKC-delta in transmitting thrombin-activated signaling i

131 3 but abolished threonine phosphorylation by PKC-delta in vitro and AD198-induced PLS3 phosphorylatio

132 f phenylalanines (Phe-500/Phe-527) unique to PKC-delta in/near the activation loop.

133 enes requires active protein kinase C-delta (PKC-delta) in addition to Smads (1).

134 the activation signature of one PKC isozyme, PKC delta, in cells, revealing unique spatial and regula

135 iRNA implicated the novel PKCs, specifically PKC-delta, in both TNF and PMA-stimulated nSMase2 transl

136 In addition, overexpression of PKC-delta increased binding of cdk inhibitor p27(Kip1) t

137 rin induces down-regulation of caspase-2 via PKC delta-independent but ubiquitin proteasome-mediated

138 gression into S phase, an effect mediated by PKC delta-induced up-regulation of the cell cycle inhibi

139 g that apoptosis was in part responsible for PKC-delta-induced growth inhibition.

140 red for apoptosis, as an uncleavable form of PKC delta induces apoptosis when retained in the nucleus

141 These studies establish that AD198-activated PKC-delta induces phosphorylation of mitochondrial PLS3

142 Finally, down-regulation of PKC-delta inhibited induction of vascular cell and inter

143 We have previously shown that the PKC delta inhibitor rottlerin protects against cisplatin

144 Coadministration of hypothalamic PKC-delta inhibitor rottlerin with OAG prevented the abi

145 These results indicate that PKC-delta inhibits transformed keratinocyte growth by in

146 tor agonist UTP induces the translocation of PKC delta into the nucleus by a mechanism that depends o

147 uring that sustained nuclear accumulation of PKC delta is coupled to caspase activation.

148 t regulation of the proapoptotic function of PKC delta is critical for cell survival.

149 Nuclear PKC delta is either cleaved by caspase 3, resulting in a

150 Full-length PKC delta is found in all cells, whereas the catalytic f

151 Nuclear accumulation of PKC delta is necessary for caspase cleavage, as mutants

152 PKC delta is recovered from the soluble fraction of H(2)

153 riments showed that in response to TGF-beta, PKC delta is recruited to the collagen promoter to phosp

154 These data provide the first evidence that PKC-delta is a critical link between p38-MAPK and Sp1-de

155 PKC-delta is a serine/threonine kinase that mediates div

156 Here we report that PKC-delta is lost in human SCCs at the transcriptional l

157 ta enhanced COX-2 expression indicating that PKC-delta is not only necessary but also sufficient to r

158 To determine if PKC-delta is one of the major alternate signaling pathwa

159 Further studies indicate that PKC-delta is required for calcium and green tea polyphen

160 A second pathway involving PKC-delta is required for neuregulin (NRG) cleavage, and

161 In this apoptotic process, PKC-delta is upregulated and translocated from the cytos

162 trated that protein kinase Cdelta (PKCdelta; PKC delta) is an oxidative stress-sensitive kinase that

163 Protein kinase C-delta (PKC-delta) is expressed in platelets and activated downs

164 Blocking the PKC delta isoform with either rottlerin, a selective ant

165 n of calcineurin blocks translocation of the PKC-delta isoform.

166 pathway, specifically involving PKC zeta and PKC delta isoforms.

167 specific inhibitors, we found that the novel PKC-delta isozyme, together with the novel PKC-epsilon a

168 inant-negative PKC delta construct (pEGFP-N1/PKC delta K376R) transfected into human bronchial epithe

169 Rather, tyrosine phosphorylation regulates PKC delta kinase activity.

170 We found that the T507A mutant of the PKC-delta kinase domain resembled the corresponding wild

171 delta levels relative to control cells; (b) PKC delta knockdown in LNCaP cells recapitulated all cha

172 Moreover, PKC-delta knockdown enhanced cell proliferation ( approx

173 PKC-delta knockdown reduces TPA-activated involucrin pro

174 recycling compartment was also impaired upon PKC-delta knockdown.

175 s in renal tubules, which was less severe in PKC-delta-knockout mice.

176 (a) PCPH knockdown in LNCaP cells decreased PKC delta levels relative to control cells; (b) PKC delt

177 PKC-delta levels are significantly higher in Tam-resista

178 ntly high levels of both total and activated PKC-delta levels compared to sensitive cells.

179 s II-4 cells that have significantly reduced PKC-delta levels.

180 Strikingly, we found that PKC delta localizes to the secretory lysosomes and polar

181 We evaluated the significance of PKC-delta loss in transformed human keratinocytes using

182 ocyte growth by inducing apoptosis, and that PKC-delta may function as a tumor suppressor in human SC

183 Thus, PKC-delta may play a critical role in the pathogenesis o

184 ults demonstrate that MMP-9 and PKC-alpha or PKC-delta may provide putative therapeutic targets for t

185 Thus, activation loop phosphorylation of PKC-delta may regulate its function in cells in a novel

186 tive PKC-delta in Tam-sensitive MCF-7 cells (PKC-delta/MCF-7) led to Tam resistance both in vitro and

187 identified in this study allow for distinct PKC delta-mediated phosphorylation events and responses

188 In this study, we show that PKC-delta-mediated activation of protein-tyrosine kinase

189 In cells PKC-delta mediates both apoptosis and transcription regu

190 Protein kinase C delta (PKC delta) mediates apoptosis downstream of many apoptot

191 omoter region of the mouse gene suggest that PKC-delta might be involved in spermatogenesis, embryoge

192 Our data suggest that CEl PKC-delta(+) neurons constitute an important node that m

193 We found that CEl PKC-delta(+) neurons in mice were activated by diverse a

194 Electrical silencing of PKC-delta(+) neurons in vivo suggests that they correspo

195 nd cell-specific viral tracing indicate that PKC-delta(+) neurons inhibit output neurons in the media

196 lso make reciprocal inhibitory synapses with PKC-delta(-) neurons in CEl.

197 In PKC-delta-null murine platelets, convulxin-induced SHIP-

198 HIP-2 or Shc, preferentially associated with PKC-delta on stimulation of platelets with a GPVI agonis

199 xpression of PKC alpha, but not PKC epsilon, PKC delta or PKC zeta isoforms, increased Ro3582-induced

200 ression of the constitutively active form of PKC-delta or Akt was sufficient to induce NF-kappaB acti

201 activation and ICAM-1 expression induced by PKC-delta or Akt.

202 Moreover, either knockout of PKC-delta or knockdown of p62 by small interfering RNA i

203 ear luminal borders, whereas the novel PKCs, PKC-delta or PKC-epsilon, displayed little or no redistr

204 ottlerin (a relatively specific inhibitor of PKC-delta) or siRNA significantly inhibited estrogen- an

205 We also observed PKC-delta, p38alpha MAPK and SHP-1 activation in brain p

206 mice with targeted disruption of PKC-delta (Pkc-delta(-)(/)(-)), p85alpha and p85beta subunits of th

207 a and PKC delta-pY(311), we demonstrate that PKC delta partitions between soluble and particulate fra

208 hear stress activates PKC delta and that the PKC delta peptide antagonist, delta V1-1, significantly

209 However, caspase cleavage of PKC delta per se is not required for apoptosis, as an un

210 In vitro PKC delta phosphorylation by Src also increases lipid-in

211 IL-7 inhibits TGF-beta-induced PKC-delta phosphorylation at Ser-645 and Thr-505.

212 for neuregulin (NRG) cleavage, and, indeed, PKC-delta phosphorylation of serine 286 in the NRG cytos

213 Here we examine two candidates for PKC-delta phosphorylation sites in the human voltage-gat

214 esentative Ser/Thr (PKC alpha, PKC betaIota, PKC delta, Pim2, Akt1, MK2, and PKA) as well as receptor

215 e constitutively active catalytic subunit of PKC delta (PKC delta-CAT), and treatment with bryostatin

216 blasts from mice with targeted disruption of PKC-delta (Pkc-delta(-)(/)(-)), p85alpha and p85beta sub

217 ptosis and that nuclear import and export of PKC delta plays a key role in regulating the survival/de

218 The results suggest that PKC delta plays an important role in mucin secretion by

219 his approach, we show that the C1b domain of PKC delta plays the predominant role in the translocatio

220 Taken together, these data suggest that PKC-delta plays a major role in antiestrogen resistance

221 independent confirmation of the direct role PKC-delta plays in cell growth and cell cycle regulation

222 Protein kinase C-delta (PKC delta) plays an important role in DNA damage-induced

223 iously reported that protein kinase C-delta (PKC-delta) plays a major role in estrogen (E2)-mediated

224 Protein kinase C-delta (PKC-delta) plays a pivotal role in mediating thrombin-in

225 We have previously shown that PKC-delta positively regulates PAR-mediated dense granul

226 as a scaffold for binding and association of PKC-delta, PP2A, and STE20-related proline-alanine-rich

227 gulation of nuclear activity; a construct of PKC delta prelocalized to the nucleus continues to be ac

228 NA levels, had significantly repressed human PKC-delta promoter activity.

229 er, these results suggest that activation of PKC-delta promotes tubular cell injury and death during

230 ic inhibition or shRNA-mediated knockdown of PKC delta protected FTY720-treated Huh7 cells from caspa

231 relevant control oligonucleotides, inhibited PKC-delta protein by more than 80% in Caco-2 cells.

232 l human epidermis and 14 human SCCs with low PKC-delta protein.

233 Protein kinase C delta (PKC-delta) protein levels are frequently low in chemical

234 regulation of MARCKS activity by Rho/ROK and PKC-delta proteins, in stimulated gut peptide secretion.

235 rogated the effect of FTY720 on facilitating PKC delta proteolysis.

236 evealing novel intracellular localization of PKC delta, providing the first example of colocalization

237 oth PMA- and H(2)O(2)-dependent increases in PKC delta-pY(311) are mediated by Src family kinases, bu

238 H(2)O(2) also increases PKC delta-pY(311) in association with its release from m

239 The PMA-dependent increase in PKC delta-pY(311) results from a lipid cofactor-induced

240 The H(2)O(2)-dependent increase in PKC delta-pY(311) results from Src activation and increa

241 lysis with antibodies to total PKC delta and PKC delta-pY(311), we demonstrate that PKC delta partiti

242 istinct tyrosine kinase activation pathways (PKC-delta/PYK2/JAK2 and metalloprotease/HB-EGF/EGF recep

243 PKC-delta re-expression suppressed the tumorigenicity of

244 expression by more than 50%, indicating that PKC-delta regulates apoptosis through Bax.

245 phorylation-acetylation cascade triggered by PKC delta represents the primary mechanism whereby TGF-b

246 ces TLR2 and -4 expression via PKC-alpha and PKC-delta, respectively, by stimulating NADPH oxidase in

247 tive reverse transcription-PCR revealed that PKC-delta RNA was reduced an average of 90% in the SCCs

248 (vi) The specific inhibitor for PKC delta, rottlerin, significantly blocked the inhibiti

249 was significantly attenuated by rottlerin, a PKC delta-selective inhibitor.

250 ly demonstrated that protein kinase C delta (PKC delta) selectively regulates TCR-induced lytic granu

251 The stimulus-specific modes for PKC delta signaling identified in this study allow for d

252 the plasma membrane (the site of most robust PKC delta signaling), Golgi, and mitochondria that is in

253 expression of a previously unknown target of PKC-delta signaling, Src homology-2 domain-containing ph

254 , as nontargeted PKC-zeta was not changed by PKC-delta siRNA oligonucleotides.

255 MARCKS phosphorylation was inhibited by PKC-delta siRNA, ROKalpha siRNA, and C3 toxin (a Rho pro

256 ibitors, MMP-9 antibody, PKC-alpha siRNA, or PKC-delta siRNA.

257 eviously demonstrated that overexpression of PKC-delta slowed the G1 progression of Caco-2 colon canc

258 lylmaleimide, and Rottlerin), PKC-alpha, and PKC-delta small interfering (si)RNAs but not by hispidin

259 y vector (EV) transfected cells, whereas the PKC-delta specific inhibitor rottlerin (3 microM) or kno

260 PKC-delta specific siRNA oligonucleotides inhibited Bax

261 PKC-delta specific siRNA oligonucleotides, but not irrel

262 ing that tyrosine phosphorylation fine-tunes PKC delta substrate specificity.

263 Rottlerin, a pharmacologic inhibitor of PKC-delta, suppressed albumin-induced Bax translocation,

264 tes to the substrate selective defect of the PKC-delta T507A mutant in cells.

265 t TGF-beta and IL-7 have opposing effects on PKC-delta; TGF-beta stimulates, while IL-7 inhibits, PKC

266 ecessary for caspase cleavage, as mutants of PKC delta that do not translocate to the nucleus are not

267 identify the nucleus as a signaling hub for PKC delta that is driven by receptor-mediated signaling

268 ucidate two critical mechanisms regulated by PKC-delta that inhibit cell cycle progression and enhanc

269 ffects mitochondrial function independent of PKC delta, thereby sensitizing cells to TRAIL, and that

270 MIE gene activation coincides with PKC-delta Thr505 phosphorylation.

271 We showed that FTY720 activated PKC delta through two distinct mechanisms: phosphorylati

272 similar patterns of expression for PCPH and PKC delta, thus strongly suggesting their likely coregul

273 The sufficiency of PKC delta to induce NF-kappa B nuclear translocation and

274 the predominant role in the translocation of PKC delta to the membrane in the presence of DAG.

275 o trigger the re-expression of pro-apoptotic PKC-delta to induce apoptosis in SCCs.

276 l pathway in which Syk signals downstream of PKC-delta to mediate thrombin induced ICAM-1 expression

277 ment where it colocalized with PKC-alpha and PKC-delta together with the endocytic recycling regulato

278 a cyclin-dependent kinase (cdk) inhibitor in PKC-delta transfectants compared with empty vector (EV)

279 Consistent with accelerated apoptosis in PKC-delta transfectants, compared to EV cells, PKC-delta

280 Here we show that full-length PKC delta transiently accumulates in the nucleus in resp

281 apoptosis, activated protein kinase C-delta (PKC-delta) translocates to mitochondria and phosphorylat

282 orbol 12-myristate 13-acetate (PMA) promotes PKC delta translocation to membranes and phosphorylation

283 Concomitantly, compared to EV control cells, PKC-delta upregulation decreased cyclin D1 and cyclin E

284 C-delta transfectants, compared to EV cells, PKC-delta upregulation increased proapoptotic regulator

285 Finally, we found that the inhibition of PKC-delta using a dominant negative plasmid significantl

286 essor actions in Caco-2 cells overexpressing PKC-delta using a Zn2+ inducible expression vector.

287 h and cell cycle regulation, we knocked down PKC-delta using specific siRNA oligonucleotides.

288 ppression of p53 expression by inhibition of PKC delta was caused by the inhibition of p53 synthesis,

289 The phosphorylation of PKC-delta was inhibited by C3 toxin, demonstrating that

290 uctures of C. elegans, mouse, rat, and human PKC-delta were analyzed, and the result revealed that PK

291 phorylations on Y-1020 of SHIP-1 and Y311 of PKC-delta were inhibited.

292 PKC-betaII, and PKC-delta, were unchanged.

293 in situ) or SCC cases had absent or reduced PKC-delta when compared to the surrounding normal epider

294 sis of Sox-based probes for PKC betaIota and PKC delta, which were previously unattainable using the

295 CsA promoted the association of PKC zeta and PKC delta with the transcription factor Sp1 as observed

296 e extent of association of the C1b domain of PKC-delta with lipids, compared with PMA or the physiolo

297 Vps34 augments the association of PKC-delta with p62 for its phosphorylation at Serine 349

298 Inhibition of PKC-delta with specific small inhibitory RNA restores TG

299 Moreover, overexpression of PKC-delta, without co-suppression of PKC-alpha and beta,

300 PKC delta-Y(311) phosphorylation does not grossly alter