ライフサイエンスコーパス: PKC-epsilon

1 erentiated cells (PKB alpha, Stat3, Src, and PKC epsilon).

2 CK1 inhibitory peptide, but it does not bind PKC epsilon.

3 ble with that required for the inhibition of PKC epsilon.

4 delta and only slightly activated by that of PKC epsilon.

5 diacylglycerol to stimulate actin binding to PKC epsilon.

6 in vitro test here by a peptide inhibitor of PKC epsilon.

7 n of the cyclin D1 promoter by PKC-alpha and PKC-epsilon.

8 ovel protein kinase C isozymes PKC-delta and PKC-epsilon.

9 itor, also had no effect, likely implicating PKC-epsilon.

10 alcium-independent novel PKCs, in particular PKC-epsilon.

11 by dominant negative mutants of PKC-alpha or PKC-epsilon.

12 ssing PKC-delta and those that overexpressed PKC-epsilon.

13 f ESO is mediated by the calcium-independent PKC-epsilon.

14 ated form of PKC-alpha and an 86-kDa form of PKC-epsilon.

15 hondrial membrane by a pathway that includes PKC-epsilon.

16 s was significantly less than that of intact PKC-epsilon.

17 The membrane PKC epsilon (41.7 +/- 4.9 versus 18.7 +/- 0.3 pmol/mg/mi

18 pmol/mg/min, four experiments, p < 0.05) and PKC epsilon (42.8 +/- 3.1 versus 19.1 +/- 3.9 pmol/mg/mi

19 wn of PKC-alpha (a classical PKC isoform) or PKC-epsilon (a novel isoform) does not inhibit these TPA

20 Protein kinase C epsilon (PKC epsilon), a member of a family of serine/threonine p

21 tified and determined the mechanism by which PKC-epsilon, a novel PKC isoform, modulates drug resista

22 pase C (PI-PLC)-gamma1, and PI-PLC-gamma2 in PKC-epsilon accumulation was assessed.

23 bitor U73122 decreased both phagocytosis and PKC-epsilon accumulation.

24 NKs, not ERK1/2; 3) PKC-zeta and -theta, not PKC-epsilon, act upstream of JNKs, ERK1/2, and NF-kappaB

25 nstitutively active mutants of PKC-alpha and PKC-epsilon activated the transactivation domain of c-Ju

26 nstitutively active mutants of PKC-alpha and PKC-epsilon activated the transactivation domain of Elk-

27 ipase C, which resulted in PI hydrolysis and PKC epsilon activation in part by stimulation of the loc

28 ponse to PMA stimulation, demonstrating that PKC epsilon activity is required for MAPK activation by

29 We have previously shown that PKC epsilon acts upstream of Akt to inhibit receptor-ini

30 Protein kinase C epsilon (PKC epsilon ) acts as an antiapoptotic protein and inhib

31 vealed an increase in myofilament-associated PKC-epsilon after PHE or ET exposure of WT preparations.

32 The particulate fraction of PKC epsilon also increased, after four 5'O/10'R cycles,

33 PKC epsilon also stimulated Raf kinase in baculovirus-in

34 nstitutively active mutants of PKC-alpha and PKC-epsilon also activated c-fos, c-jun, and cyclin E pr

35 Deletion of PKC-epsilon amino acids 222-230, encompassing a putative

36 localization, kinetics, and reversibility of PKC-epsilon anchoring in permeabilized rat cardiac myocy

37 , n = 5) prevented the translocation of both PKC epsilon and eta induced by ischemic PC, whereas the

38 that an isoform specific interaction between PKC epsilon and filamentous actin may serve as a necessa

39 creased with a conditional overexpression of PKC epsilon and increased with its knock-out by small in

40 croscopy demonstrated the co-localization of PKC epsilon and integrin beta 1 on the vesicular membran

41 We hypothesized that PKC epsilon and mitoK(ATP) interact directly to form fun

42 ent, functional association of mitochondrial PKC epsilon and mitoK(ATP).

43 The results indicate the RACK1 binds PKC epsilon and NHERF1, thus serving as a scaffold prote

44 oreactive amounts of cytosolic PKC alpha and PKC epsilon and of membrane PKC zeta were significantly

45 rotocols caused significant translocation of PKC epsilon and PKC eta isoforms from the cytosolic to t

46 PKC epsilon and PKC zeta were unchanged in KB-V1 cells;

47 owed an isoform-specific interaction between PKC epsilon and sterols, suggesting that sterols may dir

48 was enhanced by overexpression of wild-type PKC-epsilon and abolished by a dominant negative PKC-eps

49 is required for the physical association of PKC-epsilon and actin.

50 In contrast, only PKC-epsilon and conventional PKCs mediate increases in p

51 l PKC-delta isozyme, together with the novel PKC-epsilon and conventional PKCs, contributed to the ba

52 r, hepatic insulin resistance, activation of PKC-epsilon and JNK1, and defects in insulin signaling.

53 y stimulating gluconeogenesis and activating PKC-epsilon and JNK1, which may interfere with tyrosine

54 e changes were associated with activation of PKC-epsilon and JNK1.

55 ctions in diacylglycerol content and reduced PKC-epsilon and PKC-theta activity in liver and muscle r

56 signals through S1P(1) and G(i) to activate PKC-epsilon and, subsequently, a PLD2-PKC-zeta-Rac1 casc

57 pathway involving phospholipase C (PKC alpha/PKC epsilon) and intracellular calcium (PKC alpha) may c

58 ated activation of protein kinase C epsilon (PKC epsilon) and that the major PKC epsilon target is th

59 ion of protein kinase B and translocation of PKC epsilon, and it increased NO production, and these e

60 vely active mutants of PKC-alpha, PKC-delta, PKC-epsilon, and PKC-zeta to determine the roles of indi

61 expression of PKC-alpha and -delta, but not PKC-epsilon, and prevented the Ca2+-spiking responses to

62 geal cells, contraction was inhibited by the PKC-epsilon antiserum but not by antisera against other

63 erentiation (beating), while upregulation of PKC epsilon appeared to amplify differentiation (beating

64 PKC-epsilon appeared specific for mechano-sensitive ERK1

65 WT-PKC delta and WT-PKC epsilon are highly phosphorylated at activation loop

66 C-alpha, PKC-beta, PKC-gamma, PKC-delta, and PKC-epsilon are found within the cerebellum.

67 s proposed to account for a new function for PKC epsilon as part of a sterol-sensitive signal transdu

68 of phospholipase C-gamma1 and activation of PKC-epsilon as evidenced by its translocation from solub

69 activation and establish a new function for PKC-epsilon as part of a mechano-sensitive signal transd

70 ciation of phosphorylated Bad with PKC-mu or PKC-epsilon, as shown by immunoprecipitation, indicated

71 ith phorbol esters completely down-regulated PKC-epsilon, as shown by Western blots, and abolished th

72 xtent of phagocytosis, their accumulation of PKC-epsilon at the phagosome, and their sensitivity to U

73 d norepinephrine-induced phosphorylations of PKC epsilon (at the C-terminal hydrophobic motif) and PK

74 On PKC inhibition, integrin beta 1 and PKC epsilon become reversibly trapped in a tetraspanin (

75 Here we show that PKC-alpha and PKC-epsilon become associated with 14-3-3 zeta when the

76 Overexpression of PKC epsilon, but not PKC alpha, -gamma, -delta, or -zeta

77 epsilon and abolished by a dominant negative PKC-epsilon, but it was not affected by wild-type or dom

78 Constitutively active mutants of PKC-epsilon, but not of other PKC isozymes, cooperated w

79 lot analysis demonstrates that expression of PKC-epsilon, but not other PKC isoforms, is associated w

80 PKC-delta and PKC-epsilon, but not PKC-alpha or -beta, translocated fr

81 Expression of dominant-negative PKC-epsilon, but not PKC-alpha, completely inhibited VEG

82 Full-length recombinant PKC-epsilon, but not PKC-betaII, -delta, -eta, or -zeta,

83 porter plasmid, indicated that PKC-alpha and PKC-epsilon, but not PKC-delta or PKC-zeta, mediate SRE

84 Blocking PKC-epsilon, but not PKC-zeta, activity attenuated S1P-m

85 mediated PLD stimulation, demonstrating that PKC-epsilon, but not PKC-zeta, was upstream of PLD.

86 ch largely depletes PKC-alpha, PKC-beta, and PKC-epsilon, but not PKC-zeta.

87 Forced expression of PKC-epsilon by retroviral transduction was insufficient

88 , these findings indicate that PKC-alpha and PKC-epsilon can enhance the activities of at least three

89 ression of PKC epsilon in MCF-7 cells (MCF-7/PKC epsilon ) caused a decrease in p53 and an increase i

90 Depletion of Akt from MCF-7/PKC epsilon cells resulted in an increase in p53 and Bid

91 on of the novel PKC isoform, PKC epsilon, in PKC epsilon(-/-) cells is shown here to stimulate direct

92 ming phagosome in Ca-replete cells, but only PKC epsilon colocalized with phagosomes in Ca2+-depleted

93 re treated with phorbol ester only wild-type PKC-epsilon colocalized with actin in zones of cell adhe

94 tivity in immunoprecipitated c-N-Ras.c-Raf-1.PKC epsilon complexes is stimulated by PMA and is inhibi

95 fections of a dominant-negative or wild-type PKC epsilon construct did not affect either mucin secret

96 Protein kinase C-epsilon (PKC-epsilon) contains a putative actin binding motif tha

97 gth enzyme, indicating that other domains in PKC-epsilon contribute to anchoring by prolonging the bo

98 The evidence presented indicates that PKC epsilon controls an internal traffic step that under

99 nstitutively active mutants of PKC-alpha and PKC-epsilon could also induce a mutant c-fos promoter wh

100 both the regulatory and catalytic domains of PKC-epsilon could independently induce NIH3T3 transforma

101 cks the redistribution of PKC alpha, but not PKC epsilon; D609 and BAPTA do not influence the partiti

102 PKC epsilon-deficient clones were found to have lower Rh

103 epsilon delta, cells that overexpressed the PKC-epsilon delta chimera, induced a dramatically increa

104 KC-delta and -epsilon (PKC-delta epsilon and PKC-epsilon delta) were constructed by exchanging regula

105 , blocked the TPA-induced differentiation of PKC-epsilon delta-overexpressing 32D cells.

106 alphaCT1 increases Cx43-pS368 in vitro in a PKC-epsilon-dependent manner and in the IBZ in vivo acut

107 However, down-regulation of PKC-epsilon did block factor-dependent DNA synthesis qua

108 As a result, PKC-epsilon did not show pronounced specificity for PS.

109 he functional cooperation between GATA-1 and PKC-epsilon displayed dependence on cellular milieu, as

110 onstitutively active mutants of PKC-alpha or PKC-epsilon displayed increased expression of endogenous

111 orders, whereas the novel PKCs, PKC-delta or PKC-epsilon, displayed little or no redistribution in mu

112 The change in PKC epsilon distribution and in TnI phosphorylation in d

113 n, pulldown and binding assays, we show that PKC epsilon does not bind to CFTR, but does bind to a re

114 (3) PKC-epsilon, due to the lack of Ca2+ binding, cannot spe

115 protein kinase C-delta (PKC-delta), but not PKC-epsilon, enables the mouse myeloid cell line 32D to

116 an 8-amino acid sequence in the V1 region of PKC epsilon, epsilon V1-2.

117 N-terminal 144-amino-acid variable region of PKC-epsilon (epsilonV1-GFP), but not an analogous N-term

118 uggest that one potential mechanism by which PKC epsilon exerts its oncogenic activity is through der

119 d by a significant reduction in apoptosis in PKC-epsilon-expressing cells.

120 that half-maximally repressed PKC alpha and PKC epsilon expression was approximately 0.5 nmol/L.

121 Finally, down-regulation of PKC-epsilon expression by the antisense cDNA in NSCLC ce

122 PKC-epsilon expression was not significantly changed.

123 All of these events are blocked by PKC-epsilon expression.

124 induces the redistribution of PKC alpha and PKC epsilon from the soluble to the particulate compartm

125 tituted in vitro system, the dissociation of PKC epsilon from these vesicles is shown to be dependent

126 Furthermore, inhibition of PKC epsilon function either by expressing PKC epsilon(KR

127 We now report that PKC-epsilon FVB/N transgenic mice (line 215) that overex

128 PKC-epsilon-GFP bound with a striated pattern that co-lo

129 the IL-8 promoter, whereas overexpression of PKC epsilon had minor effects.

130 igonucleotides directed against PKC delta or PKC epsilon had no effect on LPL activity.

131 When compared to PKC-alpha, PKC-epsilon had lower binding affinity for PS-containing

132 Inhibition of PKC-epsilon had no effect.

133 Recently, protein kinase C epsilon (PKC epsilon) has been identified as a component of the m

134 Protein kinase C-epsilon (PKC-epsilon) has been shown to increase growth and cause

135 stitutively associated with both c-Raf-1 and PKC epsilon in a biochemically silent, but latent, signa

136 own IPC signaling components such as PKG and PKC epsilon in addition to the mitochondrial ATP-sensiti

137 in vitro, PKC alpha functioned similarly to PKC epsilon in both NIH 3T3 and COS cell assays.

138 In this study, we investigated the role of PKC epsilon in breast cancer development and progression

139 We report here that overexpression of PKC epsilon in factor-dependent human TF-1 cells extends

140 Overexpression of PKC epsilon in MCF-7 cells (MCF-7/PKC epsilon ) caused a

141 RNA interference of PKC epsilon in MDA-MB231 cells, an aggressive breast can

142 influence the partitioning of PKC alpha and PKC epsilon in normoxic myocytes.

143 lectively, these results identify a role for PKC epsilon in nPKC activation loop phosphorylations and

144 ha and PKC epsilon in the neonatal heart and PKC epsilon in the adult heart.

145 hormone specifically represses PKC alpha and PKC epsilon in the neonatal heart and PKC epsilon in the

146 Immunoblot analysis revealed the presence of PKC epsilon in the reconstitutively active fraction.

147 rotein interaction is sufficient to maintain PKC-epsilon in a catalytically active conformation.

148 n of PKC-zeta and increased the abundance of PKC-epsilon in carcinogen-induced adenomas.

149 evidence that the activation of PKC-alpha or PKC-epsilon in mouse fibroblasts can play an important r

150 Importantly, forced expression of PKC-epsilon in NCI-H82 human SCLC cells confers a signif

151 ession of either PKC-theta in MCF-7 cells or PKC-epsilon in R6 and NIH 3T3 fibroblasts had no detecta

152 , our findings suggest an important role for PKC-epsilon in regulating survival of lung cancer cells.

153 er reveals that the failure of expression of PKC-epsilon in the chemo-sensitive phenotype of small ce

154 een, lung) We suggest that overexpression of PKC-epsilon in the epidermis may lead to the induction o

155 Expression of the novel PKC isoform, PKC epsilon, in PKC epsilon(-/-) cells is shown here to

156 The particulate fraction of PKC epsilon increased in a dose-dependent fashion with t

157 Activated PKC-alpha but not PKC-epsilon induced marked phosphorylation of GDI in vit

158 PKC epsilon-induced LDL receptor transcription is indepe

159 PKC epsilon induces bcl-2 protein expression fivefold to

160 A dominant negative mutant of PKC epsilon inhibited both proliferation of NIH 3T3 cell

161 specific inhibitor peptides for PKC-delta or PKC-epsilon inhibited the DA-mediated increase in Na,K-A

162 goal of this study was to determine whether PKC epsilon interacts directly with CFTR.

163 To further establish that PKC epsilon is involved in the sterol regulation of LDL

164 ation loop but not the hydrophobic motif; DN-PKC epsilon is phosphorylated at the hydrophobic motif b

165 mma), representing 51% of this subgroup, and PKC epsilon is the most abundant among the nPKCs (delta,

166 We conclude that PKC-epsilon is neither necessary nor sufficient for Ca2+

167 med to represent activation of PKC alpha and PKC epsilon) is detectable by 1 h, sustained for up to 2

168 ious PKC inhibitors and by deficiency of the PKC epsilon isoform in a mutant T cell line.

169 In nondiabetic animals, 76% of the PKC epsilon isoform was located in the cytosol and 24% w

170 ate that up-regulation of c-myc requires the PKC-epsilon isoform and that this pathway is required fo

171 cess is mediated by both novel PKC-delta and PKC-epsilon isozymes and by conventional PKCs.

172 of PKC epsilon function either by expressing PKC epsilon(KR) or by small interfering RNA (siRNA)-medi

173 we further showed that growth inhibition by PKC epsilon(KR) required the function of p21/Cip1.

174 ase-inactive, dominant-negative PKC epsilon, PKC epsilon(KR), led to a significant inhibition of prol

175 In response to serum stimulation, PKC epsilon(KR)-expressing cells showed a prolonged G(1)

176 essive breast cancer cell line with elevated PKC epsilon levels, resulted in a cell phenotype that wa

177 ERK/MAPK signaling occurred independently of PKC-epsilon levels and correlated more closely with mega

178 T7-Tag-PKC epsilon, like native PKC epsilon, transactivated the

179 es PI-PLC-gamma1 as the enzyme that supports PKC-epsilon localization and phagocytosis.

180 epsilonV1 domain is important in determining PKC-epsilon localization and translocation kinetics in c

181 s DAG that binds to epsilonC1B, facilitating PKC-epsilon localization to phagosomes for efficient IgG

182 inhibition did not alter target ingestion or PKC-epsilon localization.

183 PKC epsilon (mainly membrane associated) and PKC zeta (b

184 ta induced by AOM, as well as to up-regulate PKC-epsilon, may underlie its ability to prevent adenoma

185 n vivo tumor growth of small interfering RNA-PKC epsilon MDA-MB231 clones was retarded by a striking

186 Interestingly, PKC epsilon-mediated induction was found to be sterol re

187 , we have examined the involvement of p53 in PKC epsilon-mediated TRAIL resistance.

188 These results suggest that PKC epsilon mediates TRAIL resistance by Akt-mediated ph

189 he trigger of this process by activating the PKC epsilon-MEK-1/2-p44/42 mitogen-activated protein kin

190 ern blot analysis revealed a 70% increase in PKC epsilon mRNA, indicating that the regulatory effects

191 WT) and dominant negative (DN) PKC delta and PKC epsilon mutants were introduced into cardiomyocyte c

192 of PKD induced by thrombin, whereas neither PKC epsilon nor PKC zeta affects thrombin-induced PKD ac

193 Scrambled PKC-epsilon oligonucleotides and antisense PKC-alpha and

194 no-sensitive ERK1/2 activation, as antisense PKC-epsilon oligonucleotides did not inhibit ERK1/2 acti

195 Knockdown of PKC epsilon or c-Myc expression using siRNA led to induc

196 PMA and PKC alpha, but not PKC epsilon or dominant negative PKC alpha, increased Ni

197 onV1.2, a peptide translocation inhibitor of PKC epsilon or PKC epsilon siRNA inhibited PMA action.

198 Removal of PKC epsilon or PKC zeta, on the other hand, did not affe

199 Overexpression of dominant negative (dn) PKC-epsilon or -zeta, but not PKC-alpha or -delta, block

200 es, but this mechanism cannot be employed by PKC-epsilon or other PKC isoforms lacking a Ca2+-binding

201 When NIH 3T3 cells overexpressing either PKC-epsilon or the deletion mutant of this isozyme were

202 Expression of PKC delta, PKC epsilon, or PKC eta causes a 10-fold increase in hIN

203 e knockdown of Bad, Bid, Akt1, Akt2, PKC-mu, PKC-epsilon, or PKC-theta was achieved by transient tran

204 utively active mutants of both PKC alpha and PKC epsilon overcame the inhibitory effects of dominant

205 und that PKC-theta but neither PKC-alpha nor PKC-epsilon participates in JNK activation, whereas all

206 Inhibition of the PKC epsilon pathway using a kinase-inactive, dominant-ne

207 acetate, hydrogen peroxide, and the specific PKC epsilon peptide agonist, psi epsilonRACK, each activ

208 prevented by chelerythrine, by the specific PKC epsilon peptide antagonist, epsilonV(1-2), and by th

209 PKC) inhibitors chelerythrine, Ro318220, and PKC-epsilon peptide antagonist epsilonV(1-2).

210 lly inhibited by transfection with antisense PKC-epsilon phosphorothioate oligonucleotides (1,000 nM

211 lly inhibited by transfection with antisense PKC epsilon phosphorothionate oligonucleotides.

212 ous actin is capable of directly stimulating PKC-epsilon phosphotransferase activity.

213 Overexpression of PKC alpha, but not PKC epsilon, PKC delta or PKC zeta isoforms, increased R

214 y using a kinase-inactive, dominant-negative PKC epsilon, PKC epsilon(KR), led to a significant inhib

215 ediated cytosol-to-membrane translocation of PKC-epsilon, PKC-delta, and PKA-alpha, -gamma, and -IIal

216 uniquely involved in cross-regulation, while PKC-epsilon, PKC-eta, and PKC-mu are not.

217 Taken together, these results revealed that PKC epsilon plays a critical and causative role in promo

218 Protein kinase C-epsilon (PKC-epsilon) plays a central role in cardiac cell signal

219 These results suggest that PKC-epsilon prevents cells from undergoing apoptosis thr

220 Antisense treatment decreased endogenous PKC epsilon protein levels and completely blocked induct

221 Down-regulation of PKC epsilon protein levels by antisense oligodeoxyribonu

222 selective 60% increase in the expression of PKC epsilon protein that corresponded to an increase in

223 nsity tissue microarray analysis showed that PKC epsilon protein was detected in 73.6% (106 of 144) o

224 mouse lines that overexpress (8- or 18-fold) PKC-epsilon protein in basal epidermal cells and cells o

225 Antisense treatment decreased PKC-epsilon protein levels by 80 +/- 13% after 72 h and

226 erexpress in epidermis approximately 18-fold PKC-epsilon protein more than their wild-type littermate

227 224) that expresses approximately eightfold PKC-epsilon protein more than their wild-type littermate

228 ed the association of Src with PKC alpha and PKC epsilon, Pyk2, and the EGF receptor.

229 ocyte hypertrophy, whereas dominant negative PKC epsilon reduced cellular viability.

230 Levels of PKC epsilon remained relatively constant during the peri

231 of two distinct PKC isozymes, PKC alpha and PKC epsilon, respectively.

232 B7-2 deficiency significantly diminished the PKC-epsilon response in APCs upon CD28-Ig stimulation.

233 lls that expressed PKC-deltaepsilon retained PKC-epsilon's full potency of tumorgenicity when injecte

234 ion studies, the isolated variable domain of PKC-epsilon selectively blocked exogenous activation of

235 mationally hidden actin binding motif in the PKC-epsilon sequence becomes exposed upon activation of

236 Also, PKC-epsilon showed reduced penetration into PS-containin

237 , these results reveal an important role for PKC epsilon signaling in lung cancer and suggest that on

238 tivating ETA receptor-phospholipase C-beta 1-PKC-epsilon signaling complexes preferentially localized

239 nduced translocation/activation of PKC alpha/PKC epsilon, since the response is slower in onset, slow

240 de translocation inhibitor of PKC epsilon or PKC epsilon siRNA inhibited PMA action.

241 Similarly, the PKC epsilon-specific activator, DCP-LA, effectively prev

242 PKC-delta- and PKC-epsilon-specific peptide agonists increased Na,K-ATP

243 In aggregate, the data suggest that PKC-epsilon specifically participates in megakaryocytic

244 Increasing PKC epsilon staining intensity was associated with high

245 Conversely, overexpression of active PKC epsilon stimulated Raf kinase activity in COS cells

246 aximally stimulated PKC enzyme activity with PKC epsilon substrate peptide (epsilon pep) but not with

247 e C epsilon (PKC epsilon) and that the major PKC epsilon target is the myristoylated, alanine-rich C-

248 n in immunoreactivity for both PKC alpha and PKC epsilon that was associated with significant reducti

249 rminus and the other to the COOH terminus of PKC-epsilon, that epsilon is present in both ferret port

250 In contrast to PKC epsilon, the translocation of PKC eta was independen

251 The binding of PKC epsilon to actin required that the kinase be activat

252 assays demonstrate dose-dependent binding of PKC epsilon to RACK1 which is inhibited by an 8-amino ac

253 te but not an inactive congener translocated PKC epsilon to the particulate fraction and produced a d

254 Ang II translocated PKC epsilon to the particulate fraction.

255 Mechanical stretch also translocated PKC epsilon to the particulate fraction; however, this w

256 timulated the translocation of PKC-alpha and PKC-epsilon to the plasma membrane.

257 of the epsilon isoform of protein kinase C (PKC-epsilon) to the membrane of a small fraction of fres

258 T7-Tag-PKC epsilon, like native PKC epsilon, transactivated the transcription of a NF-ka

259 2 to 96 hours after cytokine withdrawal, the PKC epsilon transfectants remain distributed in all phas

260 The parental and PKC-delta- and PKC-epsilon-transfected 32D cells underwent apoptosis wi

261 oportional to the level of expression of the PKC-epsilon transgene.

262 the resultant accumulation of putrescine in PKC epsilon transgenic mice are linked to growth and mai

263 Severe hair loss observed in PKC epsilon transgenic mice on DFMO during skin tumor pr

264 escine levels approximately 3-4-fold more in PKC epsilon transgenic mice than their wild-type litterm

265 DFMO treatment-associated hair loss in PKC epsilon transgenic mice was accompanied by a decreas

266 thracene (100 nmol)-TPA (5 nmol) protocol in PKC epsilon transgenic mice was completely prevented by

267 r, DFMO treatment led to marked hair loss in PKC epsilon transgenic mice.

268 neutrophils in the circulating blood in both PKC-epsilon transgenic lines.

269 ether there was an imbalance of cytokines in PKC-epsilon transgenic mice (line 215), resulting in abe

270 However, peripheral blood analyses of PKC-epsilon transgenic mice indicated significant increa

271 The susceptibility of PKC-epsilon transgenic mice to the induction of SCC and

272 oproliferative-like disease (MPD) in 100% of PKC-epsilon transgenic mice.

273 Complete pathological analysis of the second PKC-epsilon transgenic mouse (line 224) that expresses a

274 arcinoma (mSCC) in protein kinase C epsilon (PKC epsilon) transgenic mice was determined.

275 d PKC-betaII, -gamma, and -epsilon, and only PKC-epsilon translocated to the membrane fraction in res

276 Protein kinase C-epsilon (PKC-epsilon) translocates to phagosomes and promotes upt

277 ch-mediated inositol phosphate accumulation, PKC epsilon translocation is not prevented by AT1 recept

278 Moreover, PKC-epsilon translocation is not a diffusion-controlled

279 The proportion of neurones in which PKC-epsilon translocation was observed increased to arou

280 , DAG binding to epsilonC1B is necessary for PKC-epsilon translocation.

281 hexadecyl-2-acetyl glycerol (EI-150) blocked PKC-epsilon translocation.

282 topoietic cells of protein kinase C-epsilon (PKC-epsilon) up-regulation and of extracellular signal-r

283 immunohistochemistry that overexpression of PKC epsilon was detected in the vast majority (>90%) of

284 This effect of PKC epsilon was prevented by chelerythrine, by the speci

285 We demonstrated previously that PKC epsilon was required for cAMP-dependent CFTR functio

286 Kaplan-Meier analyses showed that PKC epsilon was significantly associated with poorer dis

287 LDL receptor gene transcription, endogenous PKC epsilon was specifically inhibited by transfection w

288 In contrast, myofilament-associated PKC-epsilon was decreased after PHE or ET treatment in T

289 The transgene PKC-epsilon was not detected in any of the affected orga

290 PKC-epsilon was specifically inhibited by transfection w

291 Protein kinase C-epsilon (PKC-epsilon) was previously implicated in this process b

292 the combination of both domains, as found in PKC-epsilon, was the most active form.

293 ted, whereas PKC-gamma, pT514-PKC-gamma, and PKC-epsilon were downregulated.

294 nstitutively active mutants of PKC-alpha and PKC-epsilon were the most potent activators of this repo

295 azoxide-pretreated hearts, and PKC-alpha and PKC-epsilon were translocated to sarcolemma and intercal

296 oxia-induced redistribution of PKC alpha and PKC epsilon, whereas chelation of intracellular calcium

297 n in K562 cells of protein kinase C-epsilon (PKC-epsilon), which has recently been implicated in regu

298 Artificial down-regulation of PKC-epsilon with antisense oligodeoxynucleotides blocked

299 s actin is a principal anchoring protein for PKC epsilon within intact nerve endings.

300 C-alpha, PKC-beta, PKC-gamma, PKC-delta, and PKC-epsilon) within major cerebellar cell types as well