ライフサイエンスコーパス: PPO

1 fied as substrates of the polyphenoloxidase (PPO).

2 this family, [Rh(chrysi)(phen)(PPO)](2+) (Rh-PPO).

3 is a very rich source of polyphenol oxidase (PPO).

4 sue browning are called polyphenol oxidases (PPOs).

5 belong to the family of polyphenol oxidases (PPOs).

6 enzyme, rendering it an ideal model to study PPO.

7 negative bacteria lack this oxygen-dependent PPO.

8 o be a latent precursor of the active 48 kDa PPO.

9 atant, accompanied by a 7-fold activation of PPO.

10 x by quenching the intrinsic fluorescence of PPO.

11 0min resulted in the partial inactivation of PPO.

12 he role of the 'substrate selector' in plant PPOs.

13 ylase reaction is a general functionality of PPOs.

14 The Ppo-155 beta-YAC was used to directly lipofect MEL 585 c

15 ve complex in this family, [Rh(chrysi)(phen)(PPO)](2+) (Rh-PPO).

16 ted group 1 ToPPO-2 and group 2 ToPPO-6 into PPO-2-I244R and PPO-6-R254I, respectively, and expressed

17 ibitors showed relatively weak inhibition of PPO (21.8-27.6%), even at as high as 2.0mM concentration

18 ation may be a physiological requirement for PPO-6 stability and function in vivo and raise new quest

19 We analyzed a tetrameric PPO isoenzyme (PPO-6) from dandelion (Taraxacum officinale) heterologou

20 O-2 and group 2 ToPPO-6 into PPO-2-I244R and PPO-6-R254I, respectively, and expressed them in E. coli

21 This enantio-specificity for a PPO, a representative of a widespread class of enzymes,

22 and stimulated the expression of Drosophila PPO-A1 and PPO3 in S2 cell line.

23 zymatic browning through polyphenol oxidase (PPO) action.

24 1 allows us to propose a model for localized PPO activation in insects.

25 es could be due to beta-glucosidase, POX and PPO activities changes during olive ripening and storage

26 Enzyme stability was higher than PPO activities found in other natural sources, since abo

27 nes exhibit catalase and polyphenol oxidase (PPO) activities.

28 CS decreased PPO activity and browning index of fresh cut apples and

29 Native gel stained for PPO activity in control samples showed two isoforms.

30 ns and A. fumigatus ppo mutants, implicating Ppo activity in generating PGs.

31 functionalised with thiol groups, to inhibit PPO activity in the model system and apple juice.

32 at all pressures studied caused irreversible PPO activity increase with a maximum of 6.1 fold increas

33 MPa, 60 degrees C) also caused up to 3 fold PPO activity increase.

34 PPO activity increased with ripeness and was always high

35 In Escherichia coli, PPO activity is known to be linked to respiration and th

36 her than 76 degrees C, monotonic decrease in PPO activity occurred at 0.1 MPa and pressures higher th

37 g index increased in stored eggplant fruits, PPO activity reduced in four out of eight cultivars stud

38 mutant, indicating that this region confers PPO activity to the flavodoxin.

39 PPO activity was correlated with a 48 kDa polypeptide.

40 Losses of PPO activity were favoured by the presence of EPI in mod

41 BABA-treated lettuce three bands visualising PPO activity were observed.

42 Fruit IB had positive correlation with the PPO activity, but negative correlation with TP, AC and A

43 If there is a high PPO activity, Mal d 1 could be reduced even if the total

44 e bands up to 60kDa displayed only very weak PPO activity, supporting the hypothesis that the C-termi

45 Sodium dodecyl sulphate (SDS) enhanced PPO activity, with pulp showing a stronger increase than

46 lines that display less than 5% of wild-type PPO activity.

47 n can circumvent browning problems caused by PPO activity.

48 and sodium azide showed mixed inhibition of PPO activity.

49 knockout of hemG causes a loss of measurable PPO activity.

50 extract browning and quantitative assays of PPO activity.

51 n was soluble and functional in an assay for PPO activity.

52 ith 2mM and 20mM ascorbic acid had a lowered PPO activity; compared to the control by 51% and 60%, re

53 effective inhibition of polyphenol oxidase (PPO) activity and browning in potato and apple as compar

54 s contain high levels of polyphenol oxidase (PPO) activity and o-diphenol substrates.

55 L-ascorbic acid (AA) and polyphenoloxidase (PPO) activity from Marie-Menard apple in pH 3.8 solution

56 ulp colour by inhibiting polyphenol oxidase (PPO) activity of the both tissues.

57 inia mangostana rind, on polyphenol oxidase (PPO) activity was investigated.

58 cts of Ataulfo exhibited polyphenol oxidase (PPO) activity with pyrogallol, 3-methylcatechol, catecho

59 wning Index) parameters, polyphenol oxidase (PPO) activity, ascorbic acid, gallic acid, ellagic acid,

60 lic compounds and polyphenol oxidase enzyme (PPO) activity.

61 al concentrations of herbicides that inhibit PPO also induced defence responses that conferred enhanc

62 in the green alga Chlamydomonas reinhardtii, PPO and FeC are each encoded by a single gene.

63 netic analysis indicates that C. reinhardtii PPO and FeC are most closely related to plant counterpar

64 blotting results suggest that C. reinhardtii PPO and FeC are targeted exclusively to the chloroplast,

65 s is not directly regulated by the levels of PPO and FeC in C. reinhardtii.

66 proposed that the multiplicity of genes for PPO and FeC in higher plants could be related to differe

67 The mixture of Fe2O3, PPO and glutaraldehyde was casted on the PEDOT-rGO elect

68 er perspective on the potential functions of PPO and its possible connection to cell death, we compar

69 Compared to IMC and MC, PPO and POD from OMC water showed the lowest thermal res

70 monocytogenes and maximizing inactivation of PPO and POD, with the greatest retention of bioactive co

71 ns and interfacial regions composed of mixed PPO and TFA-modified titania.

72 the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enz

73 he optimal inhibitors of polyphenol oxidase (PPO) and evaluated their effect on enzymatic browning, p

74 afety point of view while polyphenoloxidase (PPO) and peroxidase (POD) activities, total phenolic con

75 The effect of modifying polyphenol oxidase (PPO) and peroxidase (POX) activity during the extraction

76 nzymes beta-glucosidase, polyphenol oxidase (PPO) and peroxidase (POX), to determine the phenolic pro

77 le, both the activity of polyphenol oxidase (PPO) and polyphenol peroxidase (POD) in fresh-cut pineap

78 FAD-containing enzymes that is comprised of PPOs, animal MAOs, and PHDs.

79 In plants PPOs appear in gene families, and the corresponding isoe

80 The heat-released PPO appears to be identical to the enzyme previously iso

81 The posttranslationally processed PPO ( approximately 43 kDa) has a central role in the bi

82 Although plant PPOs are often discussed with regard to their role in de

83 ct by inhibiting protoporphyrinogen oxidase (PPO) are widely used to control weeds in a variety of cr

84 Prophenoloxidases (PPOs) are key enzymes of the melanization reaction, whic

85 In PPO assay, high concentrations ( 1.11 mM) of the four am

86 m metabisulfite were effective inhibitors of PPO at 1.0mM.

87 material SBA-15 adsorbs a larger quantity of PPO at pH 4.00 and offers an inhibition of enzymatic act

88 nd MCM-48) to immobilize polyphenol oxidase (PPO) at different pH has been tested.

89 We find that Rh-PPO binding induces a lesion that triggers the DNA damag

90 The PEDOT-rGO-Fe2O3-PPO biosensor was stable for at least 75 days when store

91 om its inactive precursor, prophenoloxidase (PPO), by specific proteolysis via a serine protease casc

92 Physiological parameters, SOD, POD, PPO, CAT activity, free proline, soluble protein and MDA

93 Polyphenol oxidase (PPO) catalyses oxidation of phenolics, which results in

94 Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the c

95 Protoporphyrinogen IX oxidase (PPO) catalyzes the last common step in chlorophyll and h

96 The enzyme polyphenol oxidase (PPO) catalyzes the oxidation of phenolic compounds into

97 Silencing of PPO caused major alterations in the metabolism of phenol

98 We also expressed the red clover PPO cDNAs under the control of a constitutive promoter i

99 We cloned three red clover PPO cDNAs, PPO1, PPO2, and PPO3, from a leaf cDNA librar

100 uence comparisons among the three red clover PPO clones indicated they are 87% to 90% identical at th

101 trong up-regulation of a polyphenol oxidase (PPO) coding transcript in MusaSAP1 overexpressing plants

102 nments of the TFA-modified titania, PEO, and PPO components of the hybrid were investigated.

103 chol biosensor, based on the PEDOT-rGO-Fe2O3-PPO composite modified glassy carbon (GC) electrode.

104 to firm texture and high polyphenol oxidase (PPO) content.

105 oly(ethylene oxide) (PEO-PPO-PEO) or PPO-PEO-PPO copolymer with molecular weights (MWs) from 2700 to

106 Polymerization of PPO-derived quinones causes the postharvest browning of

107 ened both enzymatic phenol oxidations before PPO deteriorated and the whole set of the chemical react

108 growing in the dark, whereas the content of PPO does not significantly differ in light- and dark-gro

109 metric assay for protoporphyrinogen oxidase (PPO, EC 1.3.3.4) activity has been developed using a 96-

110 Protoporphyrinogen oxidase (PPO, EC 1.3.3.4) catalyzes the six-electron oxidation of

111 ed within the TFA-modified titania, and that PPO environments encompass both microphase separated reg

112 of phenolic compounds and possesses a single PPO enzyme, rendering it an ideal model to study PPO.

113 ystem to further characterize the red clover PPO enzymes and PPO-mediated inhibition of postharvest p

114 gs reveal a previously unidentified class of PPO enzymes that do not utilize oxygen as an electron ac

115 ive (peroxydase, POX, and polyphenoloxydase, PPO) enzymes during olive ripening and storage and to de

116 ic isoleucine (I) at position HB2+1, group 2 PPOs exhibit a larger, positively charged arginine (R).

117 temperature range tested, polyphenoloxidase (PPO) exhibited the optimum activity at approximately 50

118 nd to obtain multiple events tolerant to the PPO family of herbicides.

119 y to physicians in each specialty by private PPOs for intermediate office visits with established pat

120 s is related to lower prices paid by private PPOs for office visits.

121 Here, we report the crystal structure of PPO from a lepidopteran insect at a resolution of 1.97 A

122 he highest affinity toward catechol, whereas PPO from BABA-elicited lettuce showed the highest affini

123 PPO from control plants demonstrated the highest affinit

124 more sensitive to the tested inhibitors than PPO from control plants.

125 PPO from Grenache grapes has recently been reported to d

126 PPO from plants elicited with BABA was also more sensiti

127 1.97 A, which is the initial structure for a PPO from the type 3 copper protein family.

128 In contrast, we have characterized PPOs from Dornfelder and Riesling grapes which display b

129 Activity of polyphenol oxidase (PPO) from "Rojo Brillante" persimmon (Diospyros kaki L.)

130 iochemical parameters of polyphenol oxidase (PPO) from lettuce leaves caused by dl-beta-amino-n-butyr

131 These findings suggest that RUNX4 regulates PPO gene expression under the control of the Toll pathwa

132 Plants expressing the antisense PPO gene presented growth alterations and their leaves s

133 ain multiple copies of the introduced mutant PPO gene.

134 only identified a single polyphenol oxidase (PPO) gene (JrPPO1).

135 ndegraded during ensiling, presumably due to PPO-generated o-quinone inhibition of leaf proteases.

136 n addition to altering spore ratios, loss of ppo genes affect natural product biosynthesis and seed c

137 NT binding motif in the promoter of mosquito PPO genes and stimulated the expression of Drosophila PP

138 The mosquito Aedes aegypti has ten PPO genes in the genome, four of which (PPO1, PPO3, PPO5

139 ould substitute functionally for A. nidulans ppo genes.

140 olation of plant protoporphyrinogen oxidase (PPO) genes and the isolation of herbicide-tolerant mutan

141 The data indicated that PPO had the highest substrate affinity for chlorogenic a

142 alysis of the current data base reveals that PPO has significant sequence similarities to mammalian m

143 leased PSII membrane-associated catalase and PPO have been purified and characterized.

144 or studies indicated that in vitro activated PPO hydroxylates Tyr inefficiently.

145 characteristics with higher influence on the PPO immobilization.

146 performance when immobilising and inhibiting PPO in model systems, and support topology is a main fac

147 metabolic changes point to a direct role for PPO in the metabolism of tyrosine and in the biosynthesi

148 t, but the native physiological functions of PPOs in undamaged, intact plant cells are not well under

149 y assessment showed that polyphenol oxidase (PPO) in all samples was more heat resistant than peroxid

150 ially purified blueberry polyphenol oxidase (PPO) in Mcllvaine buffer (pH=3.6, typical pH of blueberr

151 manner, consistent with tissue softening and PPO inactivation.

152 The first weed to evolve resistance to PPO-inhibiting herbicides was Amaranthus tuberculatus, a

153 oli grown in the presence and absence of the PPO inhibitor lactofen.

154 Treatment with the PPO inhibitor tropolone produced a twofold increase in t

155 lumioxazin, a protoporphyrinogen IX oxidase (PPO) inhibitor.

156 Evaluation of a PPO-inhibitor-resistant A. tuberculatus biotype revealed

157 n) as pre-heating treatments and addition of PPO inhibitors (citric acid, oxalic acid, and sodium bor

158 he gene, which may explain why resistance to PPO inhibitors has been rare.

159 PPO inhibitors, from natural or synthetic sources, are u

160 commercial preferred provider organization (PPO) insurance, Medicare, or Medicaid.

161 is grandiflora (AUS1) is a specialized plant PPO involved in the anabolic pathway of aurones.

162 Manduca sexta PPO is a heterodimer consisting of 2 homologous polypept

163 FTIR analysis indicated that PPO is an alpha-helix dominating enzyme.

164 Sequence analysis demonstrate that PPO is closely related to bacterial PHDs and more distan

165 sults further indicated that the activity of PPO is more important than the content of total phenols

166 Significantly, the lesion induced by Rh-PPO is not repaired in MMR-deficient cells, resulting in

167 Unlike atactic PPO, isotactic PPO is semicrystalline with a melting temperature of app

168 In this paper, hydroxy-telechelic isotactic PPO is synthesized from racemic propylene oxide with con

169 PPO is the main enzyme involved in phenolic oxidation at

170 novel catalytic reaction mechanism for plant PPOs is proposed.

171 Hydroxy-telechelic poly(propylene oxide) (PPO) is widely used industrially as a midsegment in poly

172 We analyzed a tetrameric PPO isoenzyme (PPO-6) from dandelion (Taraxacum officina

173 ns regarding distinct functions for specific PPO isoenzymes in plants.

174 code both plastid- and mitochondria-targeted PPO isoforms, allowing a mutation in a single gene to co

175 ivity, suggesting the existence of different PPO isoforms.

176 Unlike atactic PPO, isotactic PPO is semicrystalline with a melting tem

177 e first crystal structures of a latent plant PPO, its mature active and inactive form, caused by a su

178 . regia genome sequence, a second homolog of PPO (JrPPO2) was discovered.

179 The Rh-PPO mechanism is reminiscent of DNA repair enzymes that

180 characterize the red clover PPO enzymes and PPO-mediated inhibition of postharvest proteolysis in fo

181 phenotypes compared to those of other Delta ppo mutants and the wild type, including altered conidiu

182 Here, Delta ppo mutants were characterized to elucidate the role of

183 n both Aspergillus nidulans and A. fumigatus ppo mutants, implicating Ppo activity in generating PGs.

184 ignificant level of homology was observed in PPO nucleotide and conceptually translated protein seque

185 exclusion chromatography indicated that both PPOs occur as monomers with Mr of about 38kDa.

186 re, resulting from an enzymatic oxidation by PPO of the o-diphenolic compounds present in the fresh f

187 re used to give hydroxy-telechelic isotactic PPO of varying functionality and structure.

188 Polyphenoloxidases (PPO) of the type-3 copper protein family are considered

189 In plants, PPOs often occur as families of isoenzymes which are dif

190 in browning of leaf tissues from activity of PPO on the o-diphenols.

191 ighest inhibitory effect on potato and apple PPO (p 0.05).

192 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) copolymers and poly(ethylene glycol), was explo

193 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) copolymers.

194 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) or PPO-PEO-PPO copolymer with molecular weights

195 propylene glycol)-poly(ethylene glycol) (PEO-PPO-PEO) triblock copolymers (Pluronic)] could only disp

196 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymers, and their subsequent fabri

197 oxide)-poly(ethylene oxide) (PEO-PPO-PEO) or PPO-PEO-PPO copolymer with molecular weights (MWs) from

198 Theta-conditions, e.g., PS in cyclohexane or PPO/PEO in water.

199 olymer (predominantly propylene oxide based, PPO/PEO) for polar solvents or water.

200 of MusaSAP1 in biotic stress responses where PPOs perform major functions in multiple defense pathway

201 ome bioactive compounds, polyphenol oxidase (PPO), peroxidase (POX), and superoxide dismutase (SOD) e

202 acterial MAOs are no more closely related to PPOs, PHDs, and animal MAO's than they are to the unrela

203 Overall, these results suggest that PPO plays a novel and fundamental role in secondary meta

204 g applications resulted in lower activity of PPO & POD, higher DPPH radical scavenging activity, high

205 mphiphilic block copolymers based on PEO and PPO (Poloxamers and Pluronics) and advances in the area

206 ivity, phenolic content, browning index, and PPO polymorphism.

207 to induce Pin (Ser proteinase inhibitor) or PPO (polyphenol oxidase) transcripts in nonwounded leave

208 PS more than 40wt.% increased significantly PPO/POP content.

209 All the data reported indicate that PPO/POP was the major component of primary nucleus devel

210 he catalytic site: whereas group 1 dandelion PPOs possess a hydrophobic isoleucine (I) at position HB

211 nomenon that has rarely been shown for plant PPOs previously.

212 The PPO primarily engenders the enantio-specific conversion

213 We propose that Ppo products, PG, and/or other oxylipins may serve as ac

214 The expressed red clover PPO proteins were active in alfalfa extracts as evidence

215 rated fat content, but different major TAGs (PPO-, PSO-, SSO-, POP- and SOS-rich blends) were evaluat

216 nd LapA-AS lines had lower levels of Pin and PPO RNAs than wild-type controls.

217 due was identified that influences the plant PPO's acceptance or rejection of tyramine.

218 single amino acid significantly changed the PPO's substrate specificity.

219 f A. tumefaciens-mediated transformation via PPO selection enabled us to obtain single-copy transgeni

220 Transgenic alfalfa expressing red clover PPO should prove an excellent model system to further ch

221 Blueberry PPO showed a Km of 15mM and Vmax of 2.57 DeltaA420nm/min

222 PPO showed a single fraction with k60 degrees C=1.58+/-0

223 PPO showed an optimum activity at pH 6.1-6.3 and 35 degr

224 Buriti PPO showed optimum activity at pH 7.0 and 35 degrees C,

225 The A. fumigatus triple-ppo-silenced mutant was hypervirulent in the invasive pu

226 Strikingly, the PPO-silenced plants developed spontaneous necrotic lesio

227 In addition, PPO-silenced plants displayed massive (9-fold) increases

228 nscriptomes and metabolomes of wild-type and PPO-silenced plants.

229 We generated a series of PPO-silenced transgenic walnut lines that display less t

230 ence, two cultivars, which displayed highest PPO specific activity, differed in the 38 amino acid str

231 vars of eggplant were characterised by their PPO specific activity, phenolic content, browning index,

232 e dependent on both the phenolic content and PPO specific activity, whereas, total phenolic content p

233 rmation of o-quinones of EPI (QEPI) lowering PPO stability.

234 s of multifunctional linear PEG (mf-PEG) and PPO structures accessible by copolymerization of EO or P

235 The same study proposed that the PPOs' substrate specificity is controlled by one specifi

236 Polyphenol oxidases (PPOs) such as tyrosinase and laccase catalyze the enzyma

237 higher inhibitory effect on potato and apple PPO than 100 ppm citric acid.

238 uce levels of protoporphyrinogen IX oxidase (PPO), the last common enzyme of the biosynthesis of the

239 Protoporphyrinogen oxidase (PPO), the penultimate enzyme of the heme biosynthetic pa

240 stand the conformational changes of mushroom PPO, the secondary structural change of the enzyme durin

241 pact of the presence of CG, EPI and/or AA on PPO thermostability.

242 lar polymers based on poly(propylene oxide) (PPO), thymine (Thy), and diaminotriazine (DAT).

243 , which results in processing of the zymogen PPO to PO.

244 ialysable iron fraction (6.7%) more than P + PPO treatment (3.9%).

245 P + X + C + PPO treatment of the WrS blend increased the soluble non

246 ), whereas phytase + polyphenol oxidase (P + PPO) treatment only showed improvement in the TwS blend.

247 on of antibodies to B. burgdorferi using the PPO triplex test (rP100 + PepVF + rOspC-K, AUC of 0.844)

248 ctical route to hydroxy-telechelic isotactic PPO using racemic propylene oxide as the monomer.

249 One isoform of PPO was 259-fold purified using standard chromatographic

250 PPO was completely inactivated in 20min at 85 degrees C,

251 PPO was completely inactivated in pasteurised juices, wh

252 The PPO was found to be a 112 kDa homodimer.

253 In this study, Cape gooseberry PPO was isolated and biochemically characterized.

254 ecific activity of mouse liver mitochondrial PPO was measured as 0.043 nmol h-1/mg mitochondria, demo

255 The PSII membrane-associated PPO was purified by anion-exchange chromatography and wa

256 Apple PPO was thermolabile, denaturing after 10min at 70 degre

257 Polyphenol oxidase (PPO) was extracted and characterized from high-bush blue

258 Polyphenol oxidase (PPO) was extracted and characterized from ripe fruit of

259 Polyphenol oxidase (PPO) was extracted and purified from Turkish Alyanak apr

260 An enantio-specific polyphenol oxidase (PPO) was purified approximately 1,700-fold to apparent h

261 al phenol content as well as the activity of PPO were determined.

262 Three genes predicted to encode PPO were identified in A. tuberculatus.

263 leven known dandelion (Taraxacum officinale) PPOs were shown to separate into two different phylogene

264 ge enzymes, proteases and polyphenoloxidase (PPO), were determined from isothermal heat treatments of

265 onformational changes of polyphenol oxidase (PPO), which is a food quality related enzyme, after ther

266 is a very rich source of polyphenol oxidase (PPO), which negatively affects its quality upon cutting

267 Minimizing the impact of PPO with heat was critical to the extraction and recover

268 catalyst of phenols to polyphenylene oxide (PPO) with O(2) as the terminal oxidant.