ライフサイエンスコーパス: PPO

1 PPO activity increased with ripeness and was always high

2 PPO activity was correlated with a 48 kDa polypeptide.

3 PPO from control plants demonstrated the highest affinit

4 PPO from Grenache grapes has recently been reported to d

5 PPO from plants elicited with BABA was also more sensiti

6 PPO inhibitors, from natural or synthetic sources, are u

7 PPO is the main enzyme involved in phenolic oxidation at

8 PPO showed a single fraction with k60 degrees C=1.58+/-0

9 PPO showed an optimum activity at pH 6.1-6.3 and 35 degr

10 PPO was completely inactivated in 20min at 85 degrees C,

11 PPO was completely inactivated in pasteurised juices, wh

12 ic isoleucine (I) at position HB2+1, group 2 PPOs exhibit a larger, positively charged arginine (R).

13 1.97 A, which is the initial structure for a PPO from the type 3 copper protein family.

14 This enantio-specificity for a PPO, a representative of a widespread class of enzymes,

15 Evaluation of a PPO-inhibitor-resistant A. tuberculatus biotype revealed

16 or studies indicated that in vitro activated PPO hydroxylates Tyr inefficiently.

17 In addition, PPO-silenced plants displayed massive (9-fold) increases

18 leased PSII membrane-associated catalase and PPO have been purified and characterized.

19 e dependent on both the phenolic content and PPO specific activity, whereas, total phenolic content p

20 characterize the red clover PPO enzymes and PPO-mediated inhibition of postharvest proteolysis in fo

21 O-2 and group 2 ToPPO-6 into PPO-2-I244R and PPO-6-R254I, respectively, and expressed them in E. coli

22 ivity, phenolic content, browning index, and PPO polymorphism.

23 s of multifunctional linear PEG (mf-PEG) and PPO structures accessible by copolymerization of EO or P

24 mphiphilic block copolymers based on PEO and PPO (Poloxamers and Pluronics) and advances in the area

25 nments of the TFA-modified titania, PEO, and PPO components of the hybrid were investigated.

26 nd LapA-AS lines had lower levels of Pin and PPO RNAs than wild-type controls.

27 es could be due to beta-glucosidase, POX and PPO activities changes during olive ripening and storage

28 manner, consistent with tissue softening and PPO inactivation.

29 nscriptomes and metabolomes of wild-type and PPO-silenced plants.

30 Plants expressing the antisense PPO gene presented growth alterations and their leaves s

31 Apple PPO was thermolabile, denaturing after 10min at 70 degre

32 ighest inhibitory effect on potato and apple PPO (p 0.05).

33 higher inhibitory effect on potato and apple PPO than 100 ppm citric acid.

34 The PSII membrane-associated PPO was purified by anion-exchange chromatography and wa

35 Unlike atactic PPO, isotactic PPO is semicrystalline with a melting tem

36 olymer (predominantly propylene oxide based, PPO/PEO) for polar solvents or water.

37 ened both enzymatic phenol oxidations before PPO deteriorated and the whole set of the chemical react

38 Blueberry PPO showed a Km of 15mM and Vmax of 2.57 DeltaA420nm/min

39 exclusion chromatography indicated that both PPOs occur as monomers with Mr of about 38kDa.

40 Buriti PPO showed optimum activity at pH 7.0 and 35 degrees C,

41 n can circumvent browning problems caused by PPO activity.

42 re, resulting from an enzymatic oxidation by PPO of the o-diphenolic compounds present in the fresh f

43 P + X + C + PPO treatment of the WrS blend increased the soluble non

44 In contrast, we have characterized PPOs from Dornfelder and Riesling grapes which display b

45 We also expressed the red clover PPO cDNAs under the control of a constitutive promoter i

46 We cloned three red clover PPO cDNAs, PPO1, PPO2, and PPO3, from a leaf cDNA librar

47 uence comparisons among the three red clover PPO clones indicated they are 87% to 90% identical at th

48 ystem to further characterize the red clover PPO enzymes and PPO-mediated inhibition of postharvest p

49 The expressed red clover PPO proteins were active in alfalfa extracts as evidence

50 Transgenic alfalfa expressing red clover PPO should prove an excellent model system to further ch

51 In Escherichia coli, PPO activity is known to be linked to respiration and th

52 mutant, indicating that this region confers PPO activity to the flavodoxin.

53 he catalytic site: whereas group 1 dandelion PPOs possess a hydrophobic isoleucine (I) at position HB

54 CS decreased PPO activity and browning index of fresh cut apples and

55 negative bacteria lack this oxygen-dependent PPO.

56 ivity, suggesting the existence of different PPO isoforms.

57 and stimulated the expression of Drosophila PPO-A1 and PPO3 in S2 cell line.

58 Three genes predicted to encode PPO were identified in A. tuberculatus.

59 Sodium dodecyl sulphate (SDS) enhanced PPO activity, with pulp showing a stronger increase than

60 lic compounds and polyphenol oxidase enzyme (PPO) activity.

61 The mixture of Fe2O3, PPO and glutaraldehyde was casted on the PEDOT-rGO elect

62 The PEDOT-rGO-Fe2O3-PPO biosensor was stable for at least 75 days when store

63 chol biosensor, based on the PEDOT-rGO-Fe2O3-PPO composite modified glassy carbon (GC) electrode.

64 MPa, 60 degrees C) also caused up to 3 fold PPO activity increase.

65 n was soluble and functional in an assay for PPO activity.

66 proposed that the multiplicity of genes for PPO and FeC in higher plants could be related to differe

67 ation may be a physiological requirement for PPO-6 stability and function in vivo and raise new quest

68 metabolic changes point to a direct role for PPO in the metabolism of tyrosine and in the biosynthesi

69 Native gel stained for PPO activity in control samples showed two isoforms.

70 g index increased in stored eggplant fruits, PPO activity reduced in four out of eight cultivars stud

71 In this study, Cape gooseberry PPO was isolated and biochemically characterized.

72 If there is a high PPO activity, Mal d 1 could be reduced even if the total

73 ence, two cultivars, which displayed highest PPO specific activity, differed in the 38 amino acid str

74 In PPO assay, high concentrations ( 1.11 mM) of the four am

75 her than 76 degrees C, monotonic decrease in PPO activity occurred at 0.1 MPa and pressures higher th

76 ignificant level of homology was observed in PPO nucleotide and conceptually translated protein seque

77 al concentrations of herbicides that inhibit PPO also induced defence responses that conferred enhanc

78 functionalised with thiol groups, to inhibit PPO activity in the model system and apple juice.

79 performance when immobilising and inhibiting PPO in model systems, and support topology is a main fac

80 ted group 1 ToPPO-2 and group 2 ToPPO-6 into PPO-2-I244R and PPO-6-R254I, respectively, and expressed

81 at all pressures studied caused irreversible PPO activity increase with a maximum of 6.1 fold increas

82 We analyzed a tetrameric PPO isoenzyme (PPO-6) from dandelion (Taraxacum officinale) heterologou

83 Unlike atactic PPO, isotactic PPO is semicrystalline with a melting temperature of app

84 In this paper, hydroxy-telechelic isotactic PPO is synthesized from racemic propylene oxide with con

85 re used to give hydroxy-telechelic isotactic PPO of varying functionality and structure.

86 ctical route to hydroxy-telechelic isotactic PPO using racemic propylene oxide as the monomer.

87 o be a latent precursor of the active 48 kDa PPO.

88 1 allows us to propose a model for localized PPO activation in insects.

89 ith 2mM and 20mM ascorbic acid had a lowered PPO activity; compared to the control by 51% and 60%, re

90 rmation of o-quinones of EPI (QEPI) lowering PPO stability.

91 Compared to IMC and MC, PPO and POD from OMC water showed the lowest thermal res

92 knockout of hemG causes a loss of measurable PPO activity.

93 ecific activity of mouse liver mitochondrial PPO was measured as 0.043 nmol h-1/mg mitochondria, demo

94 ns and interfacial regions composed of mixed PPO and TFA-modified titania.

95 NT binding motif in the promoter of mosquito PPO genes and stimulated the expression of Drosophila PP

96 stand the conformational changes of mushroom PPO, the secondary structural change of the enzyme durin

97 ain multiple copies of the introduced mutant PPO gene.

98 atant, accompanied by a 7-fold activation of PPO.

99 g applications resulted in lower activity of PPO & POD, higher DPPH radical scavenging activity, high

100 sults further indicated that the activity of PPO is more important than the content of total phenols

101 in browning of leaf tissues from activity of PPO on the o-diphenols.

102 al phenol content as well as the activity of PPO were determined.

103 n) as pre-heating treatments and addition of PPO inhibitors (citric acid, oxalic acid, and sodium bor

104 extract browning and quantitative assays of PPO activity.

105 gs reveal a previously unidentified class of PPO enzymes that do not utilize oxygen as an electron ac

106 growing in the dark, whereas the content of PPO does not significantly differ in light- and dark-gro

107 x by quenching the intrinsic fluorescence of PPO.

108 er perspective on the potential functions of PPO and its possible connection to cell death, we compar

109 . regia genome sequence, a second homolog of PPO (JrPPO2) was discovered.

110 Minimizing the impact of PPO with heat was critical to the extraction and recover

111 monocytogenes and maximizing inactivation of PPO and POD, with the greatest retention of bioactive co

112 0min resulted in the partial inactivation of PPO.

113 ibitors showed relatively weak inhibition of PPO (21.8-27.6%), even at as high as 2.0mM concentration

114 and sodium azide showed mixed inhibition of PPO activity.

115 m metabisulfite were effective inhibitors of PPO at 1.0mM.

116 One isoform of PPO was 259-fold purified using standard chromatographic

117 s is not directly regulated by the levels of PPO and FeC in C. reinhardtii.

118 Losses of PPO activity were favoured by the presence of EPI in mod

119 Polymerization of PPO-derived quinones causes the postharvest browning of

120 material SBA-15 adsorbs a larger quantity of PPO at pH 4.00 and offers an inhibition of enzymatic act

121 We generated a series of PPO-silenced transgenic walnut lines that display less t

122 Silencing of PPO caused major alterations in the metabolism of phenol

123 Here, we report the crystal structure of PPO from a lepidopteran insect at a resolution of 1.97 A

124 FAD-containing enzymes that is comprised of PPOs, animal MAOs, and PHDs.

125 ylase reaction is a general functionality of PPOs.

126 t, but the native physiological functions of PPOs in undamaged, intact plant cells are not well under

127 leven known dandelion (Taraxacum officinale) PPOs were shown to separate into two different phylogene

128 pact of the presence of CG, EPI and/or AA on PPO thermostability.

129 Theta-conditions, e.g., PS in cyclohexane or PPO/PEO in water.

130 to induce Pin (Ser proteinase inhibitor) or PPO (polyphenol oxidase) transcripts in nonwounded leave

131 oxide)-poly(ethylene oxide) (PEO-PPO-PEO) or PPO-PEO-PPO copolymer with molecular weights (MWs) from

132 commercial preferred provider organization (PPO) insurance, Medicare, or Medicaid.

133 Protoporphyrinogen IX oxidase (PPO) catalyzes the last common step in chlorophyll and h

134 lumioxazin, a protoporphyrinogen IX oxidase (PPO) inhibitor.

135 uce levels of protoporphyrinogen IX oxidase (PPO), the last common enzyme of the biosynthesis of the

136 zymatic browning through polyphenol oxidase (PPO) action.

137 nes exhibit catalase and polyphenol oxidase (PPO) activities.

138 effective inhibition of polyphenol oxidase (PPO) activity and browning in potato and apple as compar

139 s contain high levels of polyphenol oxidase (PPO) activity and o-diphenol substrates.

140 ulp colour by inhibiting polyphenol oxidase (PPO) activity of the both tissues.

141 inia mangostana rind, on polyphenol oxidase (PPO) activity was investigated.

142 cts of Ataulfo exhibited polyphenol oxidase (PPO) activity with pyrogallol, 3-methylcatechol, catecho

143 wning Index) parameters, polyphenol oxidase (PPO) activity, ascorbic acid, gallic acid, ellagic acid,

144 he optimal inhibitors of polyphenol oxidase (PPO) and evaluated their effect on enzymatic browning, p

145 The effect of modifying polyphenol oxidase (PPO) and peroxidase (POX) activity during the extraction

146 nzymes beta-glucosidase, polyphenol oxidase (PPO) and peroxidase (POX), to determine the phenolic pro

147 le, both the activity of polyphenol oxidase (PPO) and polyphenol peroxidase (POD) in fresh-cut pineap

148 nd MCM-48) to immobilize polyphenol oxidase (PPO) at different pH has been tested.

149 Polyphenol oxidase (PPO) catalyses oxidation of phenolics, which results in

150 The enzyme polyphenol oxidase (PPO) catalyzes the oxidation of phenolic compounds into

151 trong up-regulation of a polyphenol oxidase (PPO) coding transcript in MusaSAP1 overexpressing plants

152 to firm texture and high polyphenol oxidase (PPO) content.

153 Activity of polyphenol oxidase (PPO) from "Rojo Brillante" persimmon (Diospyros kaki L.)

154 iochemical parameters of polyphenol oxidase (PPO) from lettuce leaves caused by dl-beta-amino-n-butyr

155 only identified a single polyphenol oxidase (PPO) gene (JrPPO1).

156 y assessment showed that polyphenol oxidase (PPO) in all samples was more heat resistant than peroxid

157 ially purified blueberry polyphenol oxidase (PPO) in Mcllvaine buffer (pH=3.6, typical pH of blueberr

158 Polyphenol oxidase (PPO) was extracted and characterized from high-bush blue

159 Polyphenol oxidase (PPO) was extracted and characterized from ripe fruit of

160 Polyphenol oxidase (PPO) was extracted and purified from Turkish Alyanak apr

161 An enantio-specific polyphenol oxidase (PPO) was purified approximately 1,700-fold to apparent h

162 ome bioactive compounds, polyphenol oxidase (PPO), peroxidase (POX), and superoxide dismutase (SOD) e

163 onformational changes of polyphenol oxidase (PPO), which is a food quality related enzyme, after ther

164 is a very rich source of polyphenol oxidase (PPO), which negatively affects its quality upon cutting

165 is a very rich source of polyphenol oxidase (PPO).

166 ct by inhibiting protoporphyrinogen oxidase (PPO) are widely used to control weeds in a variety of cr

167 olation of plant protoporphyrinogen oxidase (PPO) genes and the isolation of herbicide-tolerant mutan

168 Protoporphyrinogen oxidase (PPO), the penultimate enzyme of the heme biosynthetic pa

169 metric assay for protoporphyrinogen oxidase (PPO, EC 1.3.3.4) activity has been developed using a 96-

170 Protoporphyrinogen oxidase (PPO, EC 1.3.3.4) catalyzes the six-electron oxidation of

171 Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the c

172 Polyphenol oxidases (PPOs) such as tyrosinase and laccase catalyze the enzyma

173 sue browning are called polyphenol oxidases (PPOs).

174 belong to the family of polyphenol oxidases (PPOs).

175 catalyst of phenols to polyphenylene oxide (PPO) with O(2) as the terminal oxidant.

176 Hydroxy-telechelic poly(propylene oxide) (PPO) is widely used industrially as a midsegment in poly

177 lar polymers based on poly(propylene oxide) (PPO), thymine (Thy), and diaminotriazine (DAT).

178 ), whereas phytase + polyphenol oxidase (P + PPO) treatment only showed improvement in the TwS blend.

179 ialysable iron fraction (6.7%) more than P + PPO treatment (3.9%).

180 propylene glycol)-poly(ethylene glycol) (PEO-PPO-PEO) triblock copolymers (Pluronic)] could only disp

181 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) copolymers and poly(ethylene glycol), was explo

182 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) copolymers.

183 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) or PPO-PEO-PPO copolymer with molecular weights

184 y(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymers, and their subsequent fabri

185 oly(ethylene oxide) (PEO-PPO-PEO) or PPO-PEO-PPO copolymer with molecular weights (MWs) from 2700 to

186 ve complex in this family, [Rh(chrysi)(phen)(PPO)](2+) (Rh-PPO).

187 e first crystal structures of a latent plant PPO, its mature active and inactive form, caused by a su

188 is grandiflora (AUS1) is a specialized plant PPO involved in the anabolic pathway of aurones.

189 due was identified that influences the plant PPO's acceptance or rejection of tyramine.

190 Although plant PPOs are often discussed with regard to their role in de

191 novel catalytic reaction mechanism for plant PPOs is proposed.

192 nomenon that has rarely been shown for plant PPOs previously.

193 he role of the 'substrate selector' in plant PPOs.

194 In plants PPOs appear in gene families, and the corresponding isoe

195 In plants, PPOs often occur as families of isoenzymes which are dif

196 Physiological parameters, SOD, POD, PPO, CAT activity, free proline, soluble protein and MDA

197 L-ascorbic acid (AA) and polyphenoloxidase (PPO) activity from Marie-Menard apple in pH 3.8 solution

198 ge enzymes, proteases and polyphenoloxidase (PPO), were determined from isothermal heat treatments of

199 temperature range tested, polyphenoloxidase (PPO) exhibited the optimum activity at approximately 50

200 fied as substrates of the polyphenoloxidase (PPO).

201 afety point of view while polyphenoloxidase (PPO) and peroxidase (POD) activities, total phenolic con

202 Polyphenoloxidases (PPO) of the type-3 copper protein family are considered

203 ive (peroxydase, POX, and polyphenoloxydase, PPO) enzymes during olive ripening and storage and to de

204 y to physicians in each specialty by private PPOs for intermediate office visits with established pat

205 s is related to lower prices paid by private PPOs for office visits.

206 The posttranslationally processed PPO ( approximately 43 kDa) has a central role in the bi

207 om its inactive precursor, prophenoloxidase (PPO), by specific proteolysis via a serine protease casc

208 Prophenoloxidases (PPOs) are key enzymes of the melanization reaction, whic

209 the kinetic characterization of recombinant PPOs and the detection of low concentrations of this enz

210 These findings suggest that RUNX4 regulates PPO gene expression under the control of the Toll pathwa

211 netic analysis indicates that C. reinhardtii PPO and FeC are most closely related to plant counterpar

212 blotting results suggest that C. reinhardtii PPO and FeC are targeted exclusively to the chloroplast,

213 in the green alga Chlamydomonas reinhardtii, PPO and FeC are each encoded by a single gene.

214 The heat-released PPO appears to be identical to the enzyme previously iso

215 this family, [Rh(chrysi)(phen)(PPO)](2+) (Rh-PPO).

216 Significantly, the lesion induced by Rh-PPO is not repaired in MMR-deficient cells, resulting in

217 We find that Rh-PPO binding induces a lesion that triggers the DNA damag

218 The Rh-PPO mechanism is reminiscent of DNA repair enzymes that

219 Manduca sexta PPO is a heterodimer consisting of 2 homologous polypept

220 PS more than 40wt.% increased significantly PPO/POP content.

221 of phenolic compounds and possesses a single PPO enzyme, rendering it an ideal model to study PPO.

222 ns regarding distinct functions for specific PPO isoenzymes in plants.

223 enzyme, rendering it an ideal model to study PPO.

224 rated fat content, but different major TAGs (PPO-, PSO-, SSO-, POP- and SOS-rich blends) were evaluat

225 code both plastid- and mitochondria-targeted PPO isoforms, allowing a mutation in a single gene to co

226 The mosquito Aedes aegypti has ten PPO genes in the genome, four of which (PPO1, PPO3, PPO5

227 We analyzed a tetrameric PPO isoenzyme (PPO-6) from dandelion (Taraxacum officina

228 Enzyme stability was higher than PPO activities found in other natural sources, since abo

229 more sensitive to the tested inhibitors than PPO from control plants.

230 ed within the TFA-modified titania, and that PPO environments encompass both microphase separated reg

231 Sequence analysis demonstrate that PPO is closely related to bacterial PHDs and more distan

232 All the data reported indicate that PPO/POP was the major component of primary nucleus devel

233 The data indicated that PPO had the highest substrate affinity for chlorogenic a

234 FTIR analysis indicated that PPO is an alpha-helix dominating enzyme.

235 alysis of the current data base reveals that PPO has significant sequence similarities to mammalian m

236 Overall, these results suggest that PPO plays a novel and fundamental role in secondary meta

237 The PPO primarily engenders the enantio-specific conversion

238 The PPO was found to be a 112 kDa homodimer.

239 single amino acid significantly changed the PPO's substrate specificity.

240 oli grown in the presence and absence of the PPO inhibitor lactofen.

241 characteristics with higher influence on the PPO immobilization.

242 Strikingly, the PPO-silenced plants developed spontaneous necrotic lesio

243 nd to obtain multiple events tolerant to the PPO family of herbicides.

244 on of antibodies to B. burgdorferi using the PPO triplex test (rP100 + PepVF + rOspC-K, AUC of 0.844)

245 Fruit IB had positive correlation with the PPO activity, but negative correlation with TP, AC and A

246 Treatment with the PPO inhibitor tropolone produced a twofold increase in t

247 The same study proposed that the PPOs' substrate specificity is controlled by one specifi

248 vars of eggplant were characterised by their PPO specific activity, phenolic content, browning index,

249 ndegraded during ensiling, presumably due to PPO-generated o-quinone inhibition of leaf proteases.

250 he gene, which may explain why resistance to PPO inhibitors has been rare.

251 The first weed to evolve resistance to PPO-inhibiting herbicides was Amaranthus tuberculatus, a

252 acterial MAOs are no more closely related to PPOs, PHDs, and animal MAO's than they are to the unrela

253 lines that display less than 5% of wild-type PPO activity.

254 f A. tumefaciens-mediated transformation via PPO selection enabled us to obtain single-copy transgeni

255 BABA-treated lettuce three bands visualising PPO activity were observed.

256 e bands up to 60kDa displayed only very weak PPO activity, supporting the hypothesis that the C-termi

257 of MusaSAP1 in biotic stress responses where PPOs perform major functions in multiple defense pathway

258 he highest affinity toward catechol, whereas PPO from BABA-elicited lettuce showed the highest affini

259 , which results in processing of the zymogen PPO to PO.