ライフサイエンスコーパス: PRDX6

1 eroxidase and phospholipase A2 activities of Prdx6.

2 nd the glial expression of Prdx1, Prdx4, and Prdx6.

3 t the effect requires the PLA(2) activity of Prdx6.

4 Peroxiredoxin 6 (Prdx6), a bifunctional enzyme with glutathione peroxidas

5 Peroxiredoxin 6 (Prdx6), a bifunctional protein with phospholipase A2 (ai

6 We identified peroxiredoxin 6 (Prdx6), a host factor that contributes to pulmonary surf

7 ound that uterine levels of peroxiredoxin-6 (PRDX6), a unique antioxidant, are significantly lower in

8 PRDX6 activation generates reactive oxygen species via N

9 ur data point to the potential usefulness of Prdx6 activators or inhibitors for controlling different

10 lack the phospholipase A2 activity (PLA2) of Prdx6; addition of either lysophosphatidylcholine (LPC)

11 Peroxiredoxin 6 (PRDX6) (also called antioxidant protein 2, or AOP2) is a

12 ld-type cells resulted in phosphorylation of Prdx6 and its subsequent translocation from the cytosol

13 se beads, a pull-down assay using His-tagged Prdx6 and Ni(2) -chelating beads, co-immunoprecipitation

14 Recombinant human Prdx6 and SP-A isolated from human alveolar proteinosis

15 ings implicate miR-371-3p as a suppressor of PRDX6 and suggest that co-targeting of peroxiredoxin 6 o

16 se 2 and 3 (GPx2 and GPx3), peroxiredoxin 6 (Prdx6), and sulfyhydryl oxidase Q6 (Qscn6).

17 1-cysteine peroxiredoxin (1-Cys Prx, Prx VI, Prdx6, and AOP2).

18 Prdx1, Prdx2, and to a lesser extent Prdx4, Prdx6, and Trx1 are localized mainly in the nucleus of n

19 ated levels of ROS and reduced expression of PRDX6, and underwent apoptosis.

20 ioredoxins, and glutaredoxins between normal Prdx6-/- and Prdx6+/+ mice and those injected with paraq

21 e (Prdx6+/+) macrophages, and of paraquat on Prdx6-/- and Prdx6+/+ mice.

22 rogen peroxide, and t-butyl hydroperoxide on Prdx6-/- and wild-type (Prdx6+/+) macrophages, and of pa

23 We identified Prdx6 as an important player in different phases of skin

24 e showed calcium-independent SP-A binding to Prdx6 at pH 4.0 and partial Ca(2+) dependence of binding

25 Interaction of SP-A and Prdx6 at pH 7.4 was shown by Prdx6-mediated inhibition o

26 Pla cleaves Prdx6 at three distinct sites, and these cleavages disru

27 ow that the transduction of Peroxiredoxin 6 (PRDX6) attenuates TNF-alpha- and glutamate-induced RGC d

28 Selective inhibition of PRDX6 blocks Galphai depalmitoylation, prevents the enha

29 tute for Ser for the enzymatic activities of Prdx6 but not for its targeting to LB.

30 x6 null cells with wild-type and C47S mutant Prdx6, but not with mutants of the PLA(2) active site (S

31 ism for regulation of the PLA(2) activity of Prdx6 by SP-A.

32 MJ33 inhibited the PRDX6 Ca(2+)-iPLA2 activity and reduced these parameters

33 In conclusion, the inhibition of the PRDX6 Ca(2+)-iPLA2 activity promotes an oxidative stress

34 da and fusion with the oolemma were lower in Prdx6 (-/-) capacitated spermatozoa than WT capacitated

35 rf2 activator, Sulforaphane (SFN), augmented Prdx6, catalase and GSTpi expression in dose-dependent f

36 llectively, our results suggest that loss of Prdx6 caused by dysregulation of ARE/Nrf2 can be attenua

37 effect on cells or lungs from Prdx6-null or Prdx6-D140A-knock-in mice that lack the phospholipase A2

38 with either wild-type or Deltapla Y. pestis, Prdx6-deficient mice exhibit no differences in bacterial

39 also inactivates dopamine D2 receptors in a PRDX6-dependent manner.

40 PRDX6 expression is associated with poor prognosis in ca

41 mportantly, knockdown of Prdx6 revealed that Prdx6 expression was prerequisite for SFN-mediated cytop

42 load during aging was linked to a decline in Prdx6 expression.

43 32 has been identified as the active site in Prdx6 for aiPLA2 activity, and this activity was abolish

44 Results provide a rationale for use of PRDX6 for blocking ROS-mediated pathophysiology in glauc

45 Thus, while Pla is able to disrupt Prdx6 function in vitro and reduce Prdx6 levels in vivo,

46 mouse model carrying an S32T mutation in the Prdx6 gene but were absent from isolated LB.

47 Thus, PRDX6 has a critical role in the protection of the mouse

48 Prdx6 has been localized to both cytosol and lamellar bo

49 reduce Prdx6 levels in vivo, the cleavage of Prdx6 has little detectable impact on the progression or

50 ith a decrease in the levels of catalase and PRDX6 in exosomes derived from HIV-1-infected cells.

51 uamous cell carcinomas, indicating a role of Prdx6 in human skin carcinogenesis.

52 Finally, we found strong expression of PRDX6 in keratinocytes of normal human skin and in the t

53 kin tumorigenesis, whereas overexpression of Prdx6 in keratinocytes of transgenic mice had the opposi

54 Loss of Prdx6 in mice enhanced the susceptibility to skin tumori

55 ning of rat retina disclosed the presence of PRDX6 in RGCs, and Western and real-time PCR analysis re

56 ese results confirm an important role for LB Prdx6 in the degradation and remodeling of lung surfacta

57 estis reduces the abundance of extracellular Prdx6 in the lungs compared to that after infection with

58 urrent study, we aimed to define the role of PRDX6 in the pathophysiology of type 2 diabetes (T2D) us

59 tapla Y. pestis, suggesting that Pla cleaves Prdx6 in the pulmonary compartment.

60 and lower in WT spermatozoa treated with the PRDX6 inhibitor.

61 In conclusion, we demonstrated that PRDX6 is a key mediator of overt hyperglycemia in T2D gl

62 Our study provides in vivo evidence that PRDX6 is a unique non-redundant antioxidant that functio

63 We now demonstrate that Prdx6 is required for agonist-induced NOX2 activation in

64 Peroxiredoxin 6 (Prdx6) is a "moonlighting" protein with both GSH peroxid

65 Peroxiredoxin 6 (PRDX6) is a key regulator of cellular redox balance, wit

66 The antioxidant enzyme peroxiredoxin 6 (Prdx6) is a key regulator of the cellular redox balance,

67 Peroxiredoxin 6 (Prdx6) is essential for activation of NADPH oxidase type

68 wing opioid administration, peroxiredoxin 6 (PRDX6) is recruited to the opioid receptor complex by c-

69 )activity of phosphorylated peroxiredoxin 6 (Prdx6) is required for activation of NADPH oxidase (NOX2

70 thophysiology of type 2 diabetes (T2D) using PRDX6 knockout (-/-) mice.

71 dx6 transgenic mice and the higher levels in Prdx6-knockout mice than in control animals.

72 We propose that Prdx6 LB targeting facilitates its role in the metabolis

73 ffect in tumor prevention, overexpression of Prdx6 led to an acceleration of malignant progression of

74 d that Fkbp52(-/-) mice with reduced uterine PRDX6 levels are susceptible to paraquat-induced oxidati

75 o disrupt Prdx6 function in vitro and reduce Prdx6 levels in vivo, the cleavage of Prdx6 has little d

76 erine OS in Fkbp52(-/-) females with reduced PRDX6 levels induces implantation failure even in the pr

77 at Fkbp52 deficiency associated with reduced PRDX6 levels promotes H(2)O(2)-induced cell death, which

78 inishes the threshold against OS by reducing PRDX6 levels.

79 tyl hydroperoxide on Prdx6-/- and wild-type (Prdx6+/+) macrophages, and of paraquat on Prdx6-/- and P

80 Prdx6-/- macrophages had higher hydrogen peroxide levels

81 Prdx6 (-/-) male mice are subfertile, and the deficiency

82 ion of SP-A and Prdx6 at pH 7.4 was shown by Prdx6-mediated inhibition of SP-A binding to agarose bea

83 We demonstrated that PRDX6(-/-) mice developed a phenotype similar to early-s

84 Impaired insulin signaling was present in PRDX6(-/-) mice, leading to reduction of muscle glucose

85 nd glutaredoxins between normal Prdx6-/- and Prdx6+/+ mice and those injected with paraquat.

86 macrophages, and of paraquat on Prdx6-/- and Prdx6+/+ mice.

87 n peroxide levels, and lower survival rates; Prdx6-/- mice had significantly lower survival rates, mo

88 nctions, we generated Prdx6-targeted mutant (Prdx6-/-) mice, confirmed the gene disruption by Souther

89 Prdx6 mRNA was also expressed in every tissue examined.

90 ed in perfused lungs and isolated PMVEC from Prdx6 null mice.

91 after Ang II treatment in wild-type but not Prdx6 null PMVEC.

92 had much less effect on cells or lungs from Prdx6-null or Prdx6-D140A-knock-in mice that lack the ph

93 We elucidate the impact of the lack of PRDX6 or inhibition of its calcium-independent phospholi

94 sphorylated p67(phox) did not bind to either Prdx6 or phosphoPrdx6.

95 We identify PRDX6 (peroxiredoxin 6) as a key target of miR-371-3p in

96 strongly to phosphoPrdx6 but bound poorly to Prdx6; phosphorylated p67(phox) did not bind to either P

97 us, agonist-induced MAPK activation leads to Prdx6 phosphorylation and translocation to the cell memb

98 MJ33, an inhibitor of Prdx6 PLA(2) activity, blocked agonist-induced PLA(2) ac

99 y PMVECs required Prdx6-PLA2 We propose that Prdx6-PLA2 modulates NOX2 activation by generation of LP

100 Thus, Prdx6-PLA2 modulates NOX2 activation through generation

101 The generation of LPC by PMVECs required Prdx6-PLA2 We propose that Prdx6-PLA2 modulates NOX2 act

102 reduction in Nrf2/ARE binding (-357/-349) of Prdx6 promoter.

103 A supply of PRDX6 protected RGCs from glutamate and TNF-alpha induce

104 l-time PCR analysis revealed an abundance of PRDX6 protein and mRNA.

105 Prdx6 protein expression and aiPLA2 activity were normal

106 ohistochemistry, we have determined that the PRDX6 protein was widely expressed in every tissue exami

107 These findings establish JNK-dependent PRDX6 recruitment and oxidation-induced Galphai depalmit

108 Rather, endogenous and overexpressed Prdx6 reduced oxidative stress as reflected by the lower

109 Importantly, knockdown of Prdx6 revealed that Prdx6 expression was prerequisite fo

110 Target genes include HuR for miR-139-3p and Prdx6, Runx1, and Suz12 for miR-199a-3p.

111 , and lung LB was increased significantly in Prdx6-S32T mutant lungs, whereas degradation of internal

112 This study shows that FKBP52-PRDX6 signaling protects pregnancy from overt OS.

113 ilization and blastocyst rates were lower in Prdx6 (-/-) spermatozoa than in C57BL/6J wild-type (WT)

114 The widespread expression of Prdx6 suggested that its functions were quite important.

115 e addition of NAC or by forced expression of PRDX6, suggesting that Fkbp52 deficiency diminishes the

116 To determine these functions, we generated Prdx6-targeted mutant (Prdx6-/-) mice, confirmed the gen

117 nion (O2(* horizontal line )) were higher in Prdx6 (-/-) than in WT spermatozoa (p </= 0.05).

118 e that SP-A regulates the PLA(2) activity of Prdx6 through direct protein-protein interaction.

119 sections demonstrated the inability of S32T Prdx6 to bind to the chaperone protein, 14-3-3, that is

120 y was unaffected by mutation of serine 32 in Prdx6 to threonine (S32T).

121 eceptor activation recruits peroxiredoxin 6 (PRDX6) to the receptor-Galphai complex by c-Jun N-termin

122 phospholipids in the protumorigenic skin of Prdx6 transgenic mice and the higher levels in Prdx6-kno

123 recipitation of p67(phox) and phosphorylated Prdx6 was demonstrated with lysates of mouse pulmonary m

124 eraction of p67(phox) with nonphosphorylated Prdx6 was relatively weak.

125 Indeed, Prdx1, Prdx4, and Prdx6, which are expressed in glial cells in the adult C

126 dicate a direct interaction between SP-A and Prdx6, which provides a mechanism for regulation of the

127 The tumor-preventive effect of Prdx6, which was observed in a human papilloma virus 8-i

128 We investigated the interaction of Prdx6 with p67(phox) and its effect on NOX2 activity.