ライフサイエンスコーパス: PSD95

1 DZ protein, postsynaptic density protein 95 (PSD95).

2 SAP102) and postsynaptic density protein 95 (PSD95).

3 AP25 and the post-synaptic proteins NR2b and PSD95.

4 lg family, including Dlg1/SAP97, SAP102, and PSD95.

5 rane protein complex that includes Veli3 and PSD95.

6 d not interact with the other PDZ domains of PSD95.

7 tein complex with NMDA receptor subunits and PSD95.

8 idual spines where it colocalized with SAP90/PSD95.

9 oted in the postsynaptic scaffolding protein PSD95.

10 ompetitive inhibition of binding of ErbB4 to PSD95.

11 disrupt the association of KV1 channels with PSD95.

12 including synaptophysin, NMDAR1, CaMKII, and PSD95.

13 plex, decreased soluble Abeta, and increased PSD95.

14 MTAP1A to postsynaptic density molecule 95 (PSD95), a core component in the cytoarchitecture of syna

15 ncodes for post-synaptic density protein 95 (PSD95), a major synaptic protein that clusters glutamate

16 h increased the colocalization of GIRK2 with PSD95, a dendritic spine marker.

17 e hippocampal neurons and codistributes with PSD95, a major scaffolding protein of the excitatory pos

18 We also found that the PDZ1 domain of PSD95, a postsynaptic scaffolding protein, interacted wi

19 of transfected neurons expressing GFP-tagged PSD95, a prominent PSD protein, revealed that up to 40%

20 response element binding protein (CREB) and PSD95 after TBI.

21 and KA1-2 subunits of the kainate receptor; PSD95), all but two (GluR4 and KA1) were expressed at qu

22 Loss of PSD95 also depolarized cVSMCs in pressurized cerebral ar

23 hanging PSD95 palmitoylation in PSDs altered PSD95 and AMPAR levels but did not affect NMDAR levels.

24 LBP is colocalized with the synaptic marker PSD95 and is found in close proximity to processes of mi

25 ue-Dawley rats were isolated for analysis of PSD95 and K(V)1 channel proteins.

26 lored whether a specific interaction between PSD95 and KV1 channels enables protein kinase A phosphor

27 nded to reduced coimmunoprecipitation of the PSD95 and KV1 proteins without altering surface expressi

28 ohistochemistry and had higher expression of PSD95 and mGluR6 and less GFAP expression compared with

29 The subsequent decline in PSD95 and mGluR6 between 1 and 12 months in Rs1-KO retin

30 PSD95 and mGluR6 levels were normal at 1 month on Wester

31 1, the OPL was disrupted in Rs1-KO, and some PSD95 and mGluR6 was mislocalized in the outer nuclear l

32 Levels of PSD95 and mGluR6 were determined by quantitative Western

33 PSD95 and mGluR6 were juxtaposed in the OPL of the Rs1-K

34 tatory synapses that lack SHANK3 but contain PSD95 and N-methyl-D-aspartate (NMDA) receptors with fas

35 roteins can accelerate palmitate turnover on PSD95 and N-Ras.

36 a unique PDZ-PDZ domain interaction between PSD95 and nNOS.

37 ing from a protein complex of NMDA receptor, PSD95 and nNOS.

38 The synaptic proteins PSD95 and NR2B were reduced in dendritic fields and redi

39 Here, we measured PSD95 and SAP97 conformation in vitro and in postsynapti

40 Within PSDs, PSD95 and SAP97 were largely in the extended conformatio

41 he immunoreactivity of postsynaptic markers (PSD95 and spinophilin) and a presynaptic marker (syntaxi

42 he neuronal marker MAP2 and synaptic markers PSD95 and synaptophysin in AD mice was significantly rev

43 O neurons, including the scaffolding protein PSD95 and the NMDA receptors along with the known CPEB3

44 reases in the levels of post-synaptic marker PSD95 and the pre-synaptic marker synaptophysin.

45 Other palmitoylated proteins (e.g. PSD95 and Wnt) are not substrates for Hhat, and Porcupin

46 ecting the post-synaptic density protein 95 (PSD95) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepro

47 ound higher postsynaptic density protein 95 (PSD95) and lower glutamate decarboxylase 67 (GAD67) expr

48 Postsynaptic density protein 95 (PSD95) and synapse-associated protein 97 (SAP97) are hom

49 s an increase in postsynaptic density GluR1, PSD95, and actin proteins; while postsynaptic density mG

50 to a critical synaptic scaffolding molecule, PSD95, and leads it to degradation via ubiquitination.

51 sociated postsynaptic density proteins NF-L, PSD95, and SAP102 was also detected in the thalamus of s

52 d postsynaptic density proteins NF-L, PSD93, PSD95, and SAP102.

53 PSD95 appears to function as a critical 'dilator' scaffo

54 ating that the genes encoding neuroligin and PSD95 are altered in autism patients, suggest that a mol

55 We show that EO and PSD95 are necessary for SC NMDA receptor (NMDAR)-depende

56 Therefore, our study suggests that CREB and PSD95 are novel substrates of PERK, so inhibition of PER

57 reveals that copies of the synaptic protein PSD95 are synthesized in response to local activation of

58 Conclusively, this work validates PSD95 as a key player in memory and establishes epigenet

59 explored the possibility that cVSMCs express PSD95 as a scaffold to promote K(V)1 channel expression

60 the third PDZ domains of DLG1/SAP97 and DLG4/PSD95 as interaction partners for the PDZ binding motif

61 PSD95 associated with AMPARs (via transmembrane AMPAR re

62 P4 (SAPAP4), a key post-synaptic density 95 (PSD95) associated gene, truncated by the chromosome tran

63 SAP90/PSD95-associated protein 3 (SAPAP3; also known as DLGAP3

64 g phenotype caused by dysregulation of Sap90/Psd95-associated protein 3 (Sapap3; also known as Dlgap3

65 Loss of CD3zeta in brain decreased GluN2A-PSD95 association and GluN2A synaptic localization.

66 a photo-oxidizing TimeSTAMP tag reveals new PSD95 at developing dendritic structures of immature neu

67 These scaffold proteins, such as PSD95, bind to Kir2.1 channels via a PDZ-binding motif (

68 uA1 and GluA2 subunits were colocalized with PSD95 but not synapsin I, suggesting a postsynaptic loca

69 the scaffolding postsynaptic density protein PSD95, but the mode of control appears to be different f

70 young brain, and that even though SAP102 and PSD95 can bind the same NMDARs, only PSD95 enables SC sy

71 Palmitoylation of PSD95 changed its conformation from a compact to an exte

72 Treatment increased PSD95 co-immunoprecipitating with NR2B and, independent

73 The AKAP79/150-PSD95 complex is disrupted in hippocampal neurons during

74 lar and functional heterogeneity in synaptic PSD95 complexes and reveal critical roles for L27 domain

75 ns can induce the assembly and clustering of PSD95-containing postsynaptic complexes, displaying a no

76 The expression of PSD95 correlated with the expression of NR1, NR2A, NR2B,

77 th augmented association of the 5-HT2AR with PSD95 differentiated HI from LI rats.

78 response element binding protein (CREB) and PSD95 directly at the S129 and T19 residues, respectivel

79 The mechanism by which SNX27 and PSD95 discriminate these channels was previously unclear

80 his study, we report that the conserved PDZ (PSD95, Discs large, ZO-1) domain-containing protein PATJ

81 1 (NHERF-1) by binding the first of two PDZ (psd95, discs-large, ZO-1) domains.

82 ite by binding of the AQP4 C terminus to the PSD95-Discs large-ZO1 (PDZ) domain of syntrophin, a comp

83 PDZ (PSD95/Discs large/ZO-1) domains are ubiquitous protein i

84 pical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, prot

85 nduction of the wild-type protein but not of PSD95, Dlg, ZO-1 (PDZ), or leucine rich repeat domain mu

86 HtrA1 is localized to microtubules in a PDZ (PSD95, Dlg, ZO1) domain-dependent, nocodazole-sensitive

87 at contains leucine-rich repeats (LRR) and a PSD95-Dlg-Zol (PDZ) domain and that interacts specifical

88 To date, Mpdz, which encodes the multiple PSD95/DLG/ZO-1 (PDZ) domain protein (MPDZ), is the only

89 y terminus, which contains a C-terminal PDZ (PSD95/Dlg/ZO-1) binding motif; 2) blocking PDZ binding b

90 fic sequence motifs of PLC-beta and the PDZ (PSD95/Dlg/ZO-1) domains of Par proteins.

91 f the "b" splice type contain predicted PDZ (PSD95/Dlg/ZO-1) interaction domains.

92 with mutations that ablate a potential PDZ (PSD95/dlg/ZO-1) interaction motif and a putative tyrosin

93 and contains a single consensus sequence in PSD95/DLG/zo-1, which implies cross-linking of PP1 to tr

94 anger regulatory factor-2 and CFTR through a PSD95/Dlg/ZO-1-based interaction.

95 NAD is a scaffolding protein containing five PSD95/dlg/zonular occludens-1 (PDZ) domains that tether

96 tween aa 475-589, which is separate from the PSD95/dlg/zonular occludens-1 (PDZ) interacting domain.

97 ly distinct class of ligand specificity in a PSD95, DLG1, ZO-1 (PDZ) domain preferentially occurs thr

98 cling of transmembrane cargos that contain a PSD95, Dlg1, zo-1 (PDZ)-binding motif.

99 gh-risk alpha types target a select group of PSD95/DLG1/ZO1 (PDZ) domain-containing proteins by using

100 se findings show a coordinated regulation of PSD95/Dlg4 mRNA by FMRP and FXR2P that ultimately affect

101 e of FXR2P leads to decreased translation of PSD95/Dlg4 mRNA in the hippocampus, implying a role for

102 with FMRP in binding to the 3'-UTR of mouse PSD95/Dlg4 mRNA.

103 teins, such as postsynaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), z

104 102 and PSD95 can bind the same NMDARs, only PSD95 enables SC synaptic maturation.

105 We analysed the Dlg4/PSD95 epigenetic landscape in hippocampal tissue and des

106 but not ventral, striatum, cocaine decreases PSD95 expression and phosphorylation of cortactin, a cyt

107 ore, DNMT3L overexpression increased APP and PSD95 expression in differentiating neurons, whereas DNM

108 PSD95 expression level regulates AMPAR nanodomain size a

109 critical histone marks and subsequently Dlg4/PSD95 expression, which, importantly, impacted several h

110 th specificity with different members of the PSD95 family of SAPs.

111 CAPON competes with PSD95 for interaction with nNOS, and overexpression of C

112 50 (AKAP79/150) and postsynaptic density 95 (PSD95) form a complex that controls the opposing actions

113 Coimmunoprecipitation of Ggamma13 and PSD95 from brain and of Ggamma13 and SAP97 from taste ti

114 notypes of which are consistent with loss of PSD95 function.

115 ters of the scaffolding proteins gephyrin or PSD95 fused to GFP.

116 to express Post Synaptic Density 95 protein (PSD95) fused to either eGFP or mEos2 and imaged with two

117 ere transfected with a vector encoding a GFP-PSD95 fusion protein.

118 tripartite requirement of GluN2B, PSD93 and PSD95 gate the incorporation of receptors into approxima

119 pe in hippocampal tissue and designed a Dlg4/PSD95 gene-targeting strategy: a Dlg4/PSD95 zinc finger

120 port that N-terminal alternative splicing of PSD95 generates an isoform, PSD95beta that contains an a

121 PSD95-GFP fluorescent puncta represented functional syna

122 Immunoelectron microscopy confirmed that PSD95-GFP predominantly localized to ultrastructurally i

123 ment with gp120 IIIB decreased the number of PSD95-GFP puncta by 37 +/- 4%.

124 Tat (24 h) decreased the number of PSD95-GFP puncta by 50 +/- 7%.

125 ed the Tat-induced decrease in the number of PSD95-GFP puncta.

126 -gephyrin puncta and decreased the number of PSD95-GFP puncta.

127 PSD95-GFP was confirmed as a marker of excitatory input

128 otein 95 fused to green fluorescent protein (PSD95-GFP) enabled visualization of synaptic sites.

129 d a GFP-tagged postsynaptic density protein (PSD95-GFP) to visualize dendritic morphology and postsyn

130 otein 95 fused to green fluorescent protein (PSD95-GFP).

131 naptic density 95-green fluorescent protein (PSD95-GFP).

132 nsity 95 fused to green fluorescent protein (PSD95-GFP).

133 plasmid coding for the postsynaptic protein PSD95-GFP.

134 postsynaptic density (PSD) scaffold protein, PSD95, identified large (>1 microm) and irregularly shap

135 However, PSD95 immunolabeling intensities were substantially lowe

136 ying dendrites express postsynaptic density (PSD95) immunoreactive puncta suggesting that they receiv

137 opic glutamate receptor (iGluR) subunits and PSD95 in 5 precisely defined and dissected thalamic subd

138 Moreover, decrease in the loss of PSD95 in cbs(+/-) mice results in improvement of IL-1bet

139 els is enabled by a dynamic association with PSD95 in cerebral arteries and suggest that a disruption

140 However, the physiological role of PSD95 in mediating molecular signaling in cVSMCs is unkn

141 This interaction between ApoEr2 and PSD95 in neurons was modulated by NMDA receptor activati

142 The physical interaction of Ggamma13 with PSD95 in the cellular milieu was confirmed in pull-down

143 n of the synaptic proteins synaptophysin and PSD95 in the hippocampus, and prevented BCCAO-induced lo

144 of Ggamma13 abolished its interactions with PSD95 in two-hybrid and pull-down assays.

145 ts (GluR1, GluR2), or PSD protein of 95 kDa (PSD95) in either brain region.

146 Full-length PSD95 increased cell surface levels of ApoEr2 and its cl

147 all visual brain regions examined, synaptic PSD95 increases rapidly following simultaneous eyelid op

148 We also found that the PDZ2 domain of PSD95 interacted with the NR2A and NR2B subunits of NMDA

149 y of GluA1-containing AMPARs through a SAP97-PSD95 interaction.

150 AMPAR subunit delivery again through a SAP97-PSD95 interaction.

151 licated by the GPER agonist G1: G1 increased PSD95-IR in strata oriens, lucidum, and radiatum of CA3

152 Our findings provide initial evidence that PSD95 is expressed in cVSMCs, and the K(V)1 channel is o

153 Finally, we provide evidence that PSD95 is one of the substrates of ZDHHC8.

154 Postsynaptic density-95 (PSD95) is a 95 kDa scaffolding molecule in the brain tha

155 Postsynaptic density-95 (PSD95) is a scaffolding protein that associates with vol

156 Furthermore, miniature NMDAR currents after PSD95 KD show an activity-triggered calcineurin sensitiv

157 PSD95 knockdown (KD) in vivo blocks this LTP, but not lo

158 Conversely, phosphorylation of PSD95 leads to its downregulation in pericontusional cor

159 PLC is also necessary for decreases in spine PSD95 levels and AMPAR internalization.

160 PSD95 levels are diminished in ageing and neurodegenerat

161 mutant of PERK (PERK-K618A) rescues BDNF and PSD95 levels in the pericontusional cortex by reducing p

162 with internalization of AMPARs, decreases in PSD95 levels, and loss of AKAP79/150 and PKA from spines

163 tamatergic synapses, the scaffolding protein PSD95 links the neuronal isoform of nitric-oxide synthas

164 n interactions (eg, postsynaptic density 95 (PSD95)), may drive the predisposition to inherent impuls

165 While PSD95-mediated clustering was subunit independent, selec

166 These data identify PSD95 nanoclusters as a basic structural unit, or buildi

167 sub-regions revealed they comprised discrete PSD95 nanoclusters that were spatially organised into si

168 pathway of PDZ3, the third PDZ domain of the PSD95 neuronal protein, is populated by a trimeric beta-

169 ting the interaction of tau with Fyn and the PSD95-NMDA receptor complex.

170 stsynaptic sites where it interacts with the PSD95-NMDA receptor complex.

171 by regulating its ability to associate with PSD95/NMDA receptor complexes.

172 overexpression of CAPON results in a loss of PSD95/nNOS complexes in transfected cells.

173 , APP, apolipoprotein E, the synaptic marker PSD95 (now known as DLG4), the astrocyte marker GFAP, ot

174 out mice, with no change in GluA2/3, VGluT1, PSD95 or synaptophysin.

175 either the postsynaptic density (PSD) marker PSD95 or the presynaptic protein synaptophysin, as contr

176 ns, possessing either a palmitoylation site (PSD95) or an L27 domain (SAP97).

177 turnover on postsynaptic density protein 95 (PSD95) or N-Ras.

178 sms that (dys)regulate transcription of Dlg4/PSD95, or other plasticity genes, are largely unknown, l

179 PSD95 oriented perpendicular to the PSD membrane, with i

180 relatively conserved across all sub-regions, PSD95 packing into nanoclusters also varied between sub-

181 Changing PSD95 palmitoylation in PSDs altered PSD95 and AMPAR lev

182 e consistent with differential regulation of PSD95 palmitoylation in PSDs resulting from the clusteri

183 These results indicate that in PSDs, PSD95 palmitoylation, conformation, and its interactions

184 ino acids in the Kir2.1 tail associated with PSD95 PDZ1,2 by NMR spectroscopy, revealing that a stret

185 Delta414-424, completely disrupts binding to PSD95 PDZ1,2.

186 uffices to switch the binding specificity of PSD95(pdz3) quantitatively towards a class-switching lig

187 Using a PDZ domain (PSD95(pdz3)) model system, we show that sector positions

188 comprising postsynaptic density protein 95 (PSD95), PKA and its anchor AKAP150, and protein phosphot

189 structures was accompanied by appearance of PSD95-positive debris that colocalized with the processe

190 unofluorescent staining showed a 28% loss of PSD95-positive excitatory postsynaptic puncta in hippoca

191 der neurons as well as increased VGlut2- and PSD95-positive puncta, indicative of increased excitator

192 tment with PAFR antagonist BN52021 prevented PSD95-positive synapse loss in hippocampi of mice with E

193 receptor subunits GluR2 or GluR2/3 or of the PSD95 (postsynaptic density 95) family scaffolding prote

194 PSD95 (postsynaptic density protein), mGluR6 (metabotrop

195 Antisense-mediated knockdown of PSD95 profoundly reduced K(V)1 channel expression and su

196 with and stabilizes the scaffolding protein PSD95, promoting dendritic spine maturation.

197 in, TCF1, LEF1, Cyclin D1, c-myc, Wnt7a, and PSD95 protein levels 4 h later.

198 evidenced by augmented spine density, higher PSD95 protein levels, and larger miniature EPSCs.

199 We show that DLG4 (PSD95) protein is synthesised by microglia in immature m

200 DLG4 encodes postsynaptic density-95 (PSD95) protein, which plays critical roles in the format

201 DLG4, encoding the postsynaptic density-95 (PSD95) protein.

202 n conjunction with decreased neurite number, PSD95-protein levels and glutamate receptor expression.

203 rtex by reducing phosphorylation of CREB and PSD95 proteins after TBI.

204 Expression of Gad67, Nmdar1 and Psd95 proteins are also reduced.

205 In contrast, puncta levels of VGlut1 and PSD95 proteins were higher in postpubertal monkeys and p

206 or the NMDA receptor associated PSD proteins PSD95, PSD93, NF-L, and SAP102 in subjects with schizoph

207 The related MAGUKs SAP90/PSD95, PSD93/chapsyn-110, SAP97, and SAP102 all bound to

208 nrandom interval consistent with that of the PSD95 puncta on ganglion cells.

209 inor-to-major variant ratios predicted lower PSD95+ puncta density on PV interneurons.

210 Mean density of VGlut1+/PSD95+ puncta on PV+ neurons predicted the activity-depe

211 Mean density of VGlut1+/PSD95+ puncta on PV+ neurons was 18% lower in schizophre

212 lut1+) and postsynaptic density 95-positive (PSD95+) puncta, on PV interneurons was lower in postpube

213 nd postsynaptic density protein 95-positive [PSD95+] puncta) per surface area of parvalbumin-positive

214 Thus, the presence of a PSD95 punctum creates a nearby zone from which other inp

215 Veli-2, and the third PDZ domain of SAP97, a PSD95-related protein.

216 PSD95 remained tethered to presynaptic terminals in Veza

217 taining for the postsynaptic density protein PSD95 revealed that spines with high pPAK or pCofilin le

218 on; the most well-characterized being PSD93, PSD95, SAP102, and NF-L.

219 o the intercellular junction proteins dlg-A, PSD95/SAP90, SAP97, Z01, and Z02 and that contains DHR-,

220 s of mature spines and postsynaptic proteins PSD95, SAP97, GluA1, AMPAR-mediated basal synaptic trans

221 K(V)1) channel is one key binding partner of PSD95 scaffolds in neurons.

222 GluR2 and the postsynaptic protein PSD95 show progressive colocalization in tissue from P10

223 s: 1) the linear density of synaptic inputs (PSD95 sites/linear mum) varied surprisingly little and s

224 Analysis of >14,000 synapses (represented by PSD95-stained excitatory synapses) shows that there is a

225 eta metabolism, the regional distribution of PSD95 strongly correlated with the regional pattern of a

226 Members of the postsynaptic density-95 (PSD95)/synapse-associated protein-90 (SAP90) family of s

227 C1alpha and TFAM and synaptic-synaptophysin, PSD95, synapsin 1, synaptobrevin 1, neurogranin, GAP43 a

228 1alpha, TFAM (biogenesis) and synaptophysin, PSD95, synapsin 1, synaptobrevin 1, neurogranin, GAP43 a

229 Remarkably, mGluR-dependent increase of PSD95 synthesis is abolished in neurons lacking Fxr2.

230 sociation with BACE1 and neprilysin, but not PSD95 that regionally associated with full-length amyloi

231 ed specifically with the third PDZ domain of PSD95, the sole PDZ domain of Veli-2, and the third PDZ

232 e enzyme by an intermediary adaptor protein, PSD95, through a unique PDZ-PDZ domain interaction betwe

233 data indicate that young SC LTP arises from PSD95 unsilencing of silent synapses, that unsilencing i

234 MT3LshRNA could partially rescue the APP and PSD95 up-regulation in DS cells.

235 We identified PSD95, Veli-2, and other PDZ domain-containing proteins

236 ction of the artificial transcription factor PSD95-VP64 rescued memory deficits in aged and Alzheimer

237 PSD95 was detected in cVSMCs and it co-immunoprecipitate

238 ither CBS or IL-1R, IL-1beta-induced loss of PSD95 was rescued along with a decrease in the level of

239 teraction of Ggamma13 with the PDZ domain of PSD95 was via the C-terminal CAAX tail of Ggamma13 (wher

240 ErbB4 with postsynaptic density protein 95 (PSD95) was significantly reduced in the brains of BACE1(

241 Since alpha1.2 contains a binding motif for PSD95, we explored the possibility that cVSMCs express P

242 eir associated postsynaptic membrane protein PSD95 were both increased in schizophrenia in CA3 tissue

243 The synaptic genes synaptophysin and PSD95 were up-regulated, and mitochondrial function was

244 ies but reduced immunolabeling intensity for PSD95) were found in the somatosensory cortex.

245 F1, LEF1, Cyclin D1, c-myc, Wnt7a, Wnt1, and PSD95) were measured in the dorsal hippocampus 5 min or

246 DA receptor subunits NR2A and -B, as well as PSD95, were tethered to the complex.

247 alized with clusters of the scaffold protein PSD95, which are generally of larger size than AMPAR nan

248 iated guanylate kinases (MAGUKs), SAP102 and PSD95, which form a scaffold for the ion-passing glutama

249 a Dlg4/PSD95 gene-targeting strategy: a Dlg4/PSD95 zinc finger DNA-binding domain was engineered and