ライフサイエンスコーパス: PTPase 1B

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1 PTPase 1B overexpression in transfected cells was verifi

2 to insulin, protein-tyrosine phosphatase 1B (PTPase 1B) dephosphorylates 95- and 160-180-kDa tyrosine

3 cal role of protein-tyrosine phosphatase 1B (PTPase 1B) in insulin and insulin-like growth factor-I (

4 lso inhibited in cells overexpressing active PTPase 1B and enhanced in cells containing C215S PTPase

5 orylation sites within both the receptor and PTPase 1B.

6 Anti-PTPase 1B antibodies coprecipitated a 95-kDa PY protein

7 The overexpression of wild type and C215S PTPase 1B had no effects on intrinsic receptor kinase ac

8 se 1B and enhanced in cells containing C215S PTPase 1B.

9 ut was unaltered in all fractions from C215S PTPase 1B-containing cells.

10 ressing wild type or inactive mutant (C215S) PTPase 1B in cells overexpressing insulin (Hirc) or IGF-

11 To identify PTPase 1B tyrosine (Tyr) residues that are phosphorylate

12 ated cells expressing catalytically inactive PTPase 1B (CS) were immunoadsorbed and subsequently immu

13 ied by immunoblot analysis with a monoclonal PTPase 1B antibody.

14 1B and increased in cells expressing mutant PTPase 1B, in comparison with parental cells.

15 The effects of PTPase 1B overexpression on cellular protein tyrosine ph

16 ing various combinations of phosphotyrosine, PTPase 1B, and insulin receptor (IR) antibodies.

17 i-IR antibodies coprecipitated the 50-kDa PY-PTPase 1B protein from insulin-treated cells.

18 153 significantly reduced insulin-stimulated PTPase 1B phosphotyrosine content, as well as its associ

19 These data strongly suggest that PTPase 1B acts as a negative regulator of insulin and IG

20 These results suggest that PTPase 1B complexes with the autophosphorylated insulin

21 IR autophosphorylation is necessary for the PTPase 1B-IR interaction.

22 We also found proteolytic cleavage of the PTPase 1B (PTP1B) in Jurkat cells after contact with ame

23 inhibited in cells overexpressing wild type PTPase 1B and increased in cells expressing mutant PTPas

24 1 fraction of cells overexpressing wild type PTPase 1B was 3-6-fold higher than parental cells but wa

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