ライフサイエンスコーパス: PVX

1 PVX can also infect Arabidopsis ago2 mutants, albeit les

2 PVX RNA-host protein complex formation occurs in vitro a

3 re tobacco plants co-expressing HC-Pro and a PVX/GUS amplicon accumulate GUS to approximately 3% of t

4 f gene silencing in seven lines expressing a PVX RNA that was unable to replicate.

5 s inoculated with transcripts derived from a PVX cDNA clone or from clones containing mutations in th

6 tivates expression of a reporter gene from a PVX subgenomic promoter.

7 expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of g

8 el of beta-glucuronidase (GUS) activity in a PVX/GUS line is similar to that in some transgenic lines

9 while PVX is normally virulent on tomato, a PVX derivative expressing avrPto was only capable of inf

10 transcriptional gene silencing (PTGS) with a PVX amplicon carrying a gene encoding L1, and targeting

11 t expression of HopPtoN via infection with a PVX viral vector enabled tomato and N. benthamiana plant

12 , extreme strain-specific resistance against PVX, low and non-uniform GUS expression (in the PVX/GUS

13 with anaemia + PVX (28 +/- 4%) and anaemia + PVX + hypoxia (46 +/- 6%) and decreased with polycythaem

14 a + plasma volume expansion (PVX), anaemia + PVX + hypoxia, polycythaemia, polycythaemia + hyperoxia

15 However, LBF increased with anaemia + PVX (28 +/- 4%) and anaemia + PVX + hypoxia (46 +/- 6%)

16 LBF and Q with anaemia + PVX + hypoxia (8.0 +/- 0.5 and 15.8 +/- 0.7 l min(-1), r

17 nt for the coat protein gene (PVX/GUS/CP and PVX/GUS respectively).

18 n-uniform GUS expression (in the PVX/GUS and PVX/GUS/CP plants) and suppression of transiently expres

19 amplicons, were the intact genome of PVX and PVX constructs modified to carry the beta-glucuronidase

20 ssembly on DNA in planta unless activated by PVX.

21 aking interneuron PVY and its auxiliary cell PVX.

22 ppressor of silencing that appears to enable PVX to infect Arabidopsis.

23 anaemia, anaemia + plasma volume expansion (PVX), anaemia + PVX + hypoxia, polycythaemia, polycythae

24 he degradation of viral RNA as was found for PVX carrying the complete gfp cDNA.

25 ze class of siRNA produced and, at least for PVX, was not dependent on the presence of the virus-enco

26 ular type induced by TGBp2 are necessary for PVX plasmodesmata transport.

27 s thaliana), which is normally a nonhost for PVX, if coinfected with a second virus, Pepper ringspot

28 We suggest that primary siRNAs produced from PVX and PPV in the absence of RDR6 may not be good effec

29 as a replacement for the coat protein gene (PVX/GUS/CP and PVX/GUS respectively).

30 infected with tobacco mosaic virus, and (ii) PVX virus-induced gene silencing of WIPK attenuated N ge

31 elf, is sufficient to induce the increase in PVX pathogenicity and that both P1 and P3 coding sequenc

32 ontaining sequences typical of the two major PVX subgenomic RNAs.

33 1/HC-Pro region prolongs the accumulation of PVX (-) strand RNA and transactivates expression of a re

34 increased pathogenicity and accumulation of PVX are mediated by the expression of potyviral 5' proxi

35 ures of SL1 are critical for accumulation of PVX plus-strand RNA.

36 In the case of PVX-potyviral synergism, we show that the expression of

37 nly expression of HC-Pro, the enhancement of PVX (-) strand RNA accumulation in protoplasts is signif

38 Unlike the synergistic enhancement of PVX pathogenicity, which requires only expression of HC-

39 Expression of PVX TGBp3 from a heterologous vector induces the same se

40 here as amplicons, were the intact genome of PVX and PVX constructs modified to carry the beta-glucur

41 Steady-state levels of PVX replicase and TGBp2 (which reside in the ER) protein

42 ar upstream elements; NUEs) in the 3' NTR of PVX RNA were more important for plus-strand RNA accumula

43 compartmentalize the viral gene products of PVX infection.

44 nd AGO5 are required for full restriction of PVX infection in systemic tissues of Arabidopsis.

45 Furthermore, we find that restriction of PVX on Arabidopsis also depends on AGO2, suggesting that

46 rsions of Rx are resistant to the strains of PVX and the PopMV that previously caused trailing necros

47 d within the 3' untranslated region (UTR) of PVX genomic RNA and that an 8-nucleotide U-rich sequence

48 PVY/PVX preferentially activate the backward, and not forwar

49 Unlike many sex-shared pathways, PVY/PVX regulate the command cells via cholinergic, rather t

50 HRs triggered by the gene-for-gene pairs Rx1/PVX-CP and Sto1/IpiO1.

51 as LRR mutants protect against both a second PVX strain and the distantly related poplar mosaic virus

52 U-rich region from the 3' UTR of a sensitive PVX reporter virus that carries the luciferase gene in p

53 us X (PVX) RNA is identical in all sequenced PVX strains and contains sequences that are conserved am

54 supported by covariation analysis of several PVX strains, the functional significance of this structu

55 n indicated that both minus- and plus-strand PVX RNAs were detectable at 0.5 h postinoculation.

56 ry function because their failure to support PVX movement could be complemented by heterologous suppr

57 n inoculated leaves was reduced and systemic PVX accumulation was altered.

58 is AGO proteins have the potential to target PVX lacking its viral suppressor of RNA silencing (VSR),

59 These data demonstrate that PVX amplicons present a novel approach for the consisten

60 We also show that PVX is able to infect Arabidopsis Dicer-like mutants, in

61 s disease resistance gene, Gpa2, showed that PVX recognition localized to the C-terminal half of the

62 The PVX TGBp2 protein induces vesicles of the granular type

63 e genome of potato virus X (PVX) allowed the PVX RNA to be localized.

64 ted in GFP:TGBp2 studies were induced by the PVX TGBp2 protein.

65 X (PVX) TGBp2 gene, inserted into either the PVX infectious clone or pRTL2 plasmids, and used to stud

66 CC-NBS-LRR protein Rx and its elicitor, the PVX coat protein (CP), results in a rapid HR.

67 in transgenic tobacco leaves expressing the PVX coat protein elicitor of Rx2-mediated resistance.

68 infected cells following expression from the PVX vector.

69 These caps harbored the PVX replicase and nonencapsidated vRNA and represented P

70 hese results identify a novel element in the PVX TGBp2 protein which determines vesicle morphology.

71 Short gfp sequences in the PVX vector provided as effective a target for the degrad

72 , low and non-uniform GUS expression (in the PVX/GUS and PVX/GUS/CP plants) and suppression of transi

73 the same mutation, when introduced into the PVX reporter virus, eliminates viral multiplication.

74 carries the luciferase gene in place of the PVX coat protein gene results in a more than 70,000-fold

75 e leucine rich repeats in recognition of the PVX coat protein.

76 Optical sectioning of the PVX VRCs revealed that one of the viral movement protein

77 Here, we show that TGB1 organizes the PVX "X-body," a virally induced inclusion structure, by

78 These data indicate that the PVX 3' NTR contains multiple, overlapping elements that

79 Unlike TMV, the PVX RNA was concentrated in distinctive 'whorls' within

80 n fluorescent protein (GFP) was fused to the PVX TGBp2 coding sequence and inserted into the viral ge

81 med in the nuclei of cells infected with the PVX vector containing the N gene.

82 hat the 8-nucleotide U-rich motif within the PVX 3' UTR is important for some aspect of viral multipl

83 The dependence of TMV, PVX, and TBSV on intact microfilaments for intercellular

84 these sequence and/or structural elements to PVX RNA accumulation was further analyzed by inoculation

85 erminal deletion mutant showed resistance to PVX infection, while transgenic plants expressing very h

86 nsible for Arabidopsis nonhost resistance to PVX.

87 encing compromises Rx-mediated resistance to PVX.

88 active site mutant PAP were not resistant to PVX.

89 Taken together, PVX TGBp3-induced ER stress leads to up-regulation of bZ

90 y AGO2 and AGO5 are able to target wild-type PVX.

91 In addition, by using PVX-induced gene silencing, we demonstrated that the sup

92 nthesis of four major, high-molecular-weight PVX RNA products (R1 to R4).

93 We found that while PVX is normally virulent on tomato, a PVX derivative exp

94 of RNA levels in protoplasts inoculated with PVX clones containing deletions or an insertion in their

95 In protoplasts and plants inoculated with PVX-GFP:TGBp2 or transfected with pRTL2-GFP:TGBp2, fluor

96 t combining data mining using PexFinder with PVX-based functional assays can facilitate the discovery

97 of Nicotiana tabacum (NT-1) protoplasts with PVX transcripts containing mutations in the 3' NTR.

98 Protoplasts transfected with PVX-GFP:TGBp2 or pRTL2-GFP:TGBp2 were treated with cyclo

99 utation analysis of the Potato Potexvirus X (PVX) silencing suppressor P25, we provide evidence that

100 s goal was the production of potato virus X (PVX) "amplicon" lines: transgenic lines that encode a re

101 sequences into the genome of potato virus X (PVX) allowed the PVX RNA to be localized.

102 For the potexvirus Potato virus X (PVX) and the potyvirus Plum pox virus (PPV), the efficie

103 Here we show that Potato virus X (PVX) can infect Arabidopsis (Arabidopsis thaliana), whic

104 expression of a replicating potato virus X (PVX) construct (termed an 'amplicon') reproducibly and c

105 Potato virus X (PVX) does not systemically infect Arabidopsis thaliana C

106 s binary vector carrying the potato virus X (PVX) genome.

107 site within the 3' region of potato virus X (PVX) genomic RNA.

108 Infection with Potato virus X (PVX) in Nicotiana benthamiana plants leads to increased

109 cting sequences required for potato virus X (PVX) replication.

110 Potato virus X (PVX) requires three virally encoded proteins, the triple

111 n-translated region (NTR) of potato virus X (PVX) RNA is identical in all sequenced PVX strains and c

112 83), within the 5' 230 nt of potato virus X (PVX) RNA.

113 ne was a cDNA of replicating potato virus X (PVX) RNA.

114 (GFP) gene was fused to the potato virus X (PVX) TGBp2 gene, inserted into either the PVX infectious

115 dually from the heterologous potato virus X (PVX) vector, both proteins preferentially accumulated in

116 Using a recombinant potato virus X (PVX) vector, we investigated the relationship between th

117 usceptible plant lines via a potato virus X (PVX) vector.

118 h the plus-strand RNA virus, potato virus X (PVX), supported synthesis of four major, high-molecular-

119 co leaves in the presence of potato virus X (PVX), the C-terminal deletion mutant had antiviral activ

120 which confers resistance to Potato virus X (PVX), to investigate the function of the ARC region.

121 [tobacco mosaic virus (TMV), potato virus X (PVX), tomato bushy stunt virus (TBSV)], is inhibited by

122 e against a single strain of potato virus X (PVX), whereas LRR mutants protect against both a second

123 potato confers resistance to potato virus X (PVX).

124 ene block (TGB), typified by Potato virus X (PVX).

125 2 confers resistance against potato virus X (PVX).

126 not in plants infected with potato virus X (PVX).

127 In potato virus X (PVX)/potyviral synergism, increased pathogenicity and ac