ライフサイエンスコーパス: Pichia pastoris

1 garicus meleagris recombinantly expressed in Pichia pastoris .

2 se by PCR, and the mutants were expressed in Pichia pastoris.

3 ation of human ABC transporters in the yeast Pichia pastoris.

4 strate membrane insertion in both E.coli and Pichia pastoris.

5 ylase has now been successfully expressed in Pichia pastoris.

6 pexophagy of methanol-induced peroxisomes in Pichia pastoris.

7 mbinant protein expressed in and secreted by Pichia pastoris.

8 secretion pathway of the methylotropic yeast Pichia pastoris.

9 es domain 5 alone (Dom5His) was expressed in Pichia pastoris.

10 sed as a recombinant proprotein in the yeast Pichia pastoris.

11 d at discrete tER sites in the budding yeast Pichia pastoris.

12 ase (nXGase) were produced heterologously in Pichia pastoris.

13 and expressed recombinant F2 in E. coli and Pichia pastoris.

14 ne and cytoplasmic domains, was expressed in Pichia pastoris.

15 sed recombinant human clusterin in the yeast Pichia pastoris.

16 ified recombinant Epo (R103A) from the yeast Pichia pastoris.

17 albumin 8 (SFA8) in the methylotrophic yeast Pichia pastoris.

18 1, PH2, and PH4 proteins after expression in Pichia pastoris.

19 a soluble, secretory protein using the yeast Pichia pastoris.

20 ADE1, and URA3-from the methylotrophic yeast Pichia pastoris.

21 nds retinal after heterologous expression in Pichia pastoris.

22 inant form of AChE C was highly expressed by Pichia pastoris.

23 amples of human growth hormone secreted from Pichia pastoris.

24 ve (pex) mutants of the methylotrophic yeast Pichia pastoris.

25 acterization of the FLD1 gene from the yeast Pichia pastoris.

26 Z2 formed single rings in cells of the yeast Pichia pastoris.

27 deletion mutants were expressed in the yeast Pichia pastoris.

28 n both chinese hamster ovary (CHO) cells and Pichia pastoris.

29 uired for peroxisome biogenesis in the yeast Pichia pastoris.

30 and the recombinant protein was expressed in Pichia pastoris.

31 ed 50% of the total supernatant protein from Pichia pastoris.

32 C-terminal half of VCP has been expressed in Pichia pastoris.

33 hate dehydrogenase gene (GAP) from the yeast Pichia pastoris.

34 in and secreted by the methylotrophic yeast Pichia pastoris.

35 roxisome assembly (pas) mutants in the yeast Pichia pastoris.

36 aised to the recombinant protein produced in Pichia pastoris.

37 equired for peroxisome assembly in the yeast Pichia pastoris.

38 milar cleavage-secretion of ACE in the yeast Pichia pastoris.

39 isome biogenesis in the methylotrophic yeast Pichia pastoris.

40 ytic domain of CA IX in methylotrophic yeast Pichia pastoris.

41 myces boulardii, Saccharomyces paradoxus, or Pichia pastoris.

42 We tested these ideas using the yeast Pichia pastoris.

43 mh2 was heterologously produced in the yeast Pichia pastoris.

44 roduced in 293T cells, Escherichia coli, and Pichia pastoris.

45 llus subtilis xylanase A (XynA) expressed in Pichia pastoris.

46 duced by recombinant expression in the yeast Pichia pastoris.

47 r protein expression in Escherichia coli and Pichia pastoris.

48 The abf3 gene was thus expressed in Pichia pastoris.

49 iogenesis genes in the methylotrophic yeast, Pichia pastoris.

50 ence selected for heterologous expression in Pichia pastoris.

51 CD4 chimeric proteins in mammalian cells and Pichia pastoris.

52 g dominant-negative mutant forms of Sar1p in Pichia pastoris.

53 ltration of recombinant proteins produced by Pichia pastoris.

54 In this work, nicaTf was expressed in Pichia pastoris.

55 Human IgG1-Fc was first overproduced in Pichia pastoris.

56 ed in the methylotrophic yeast Komagataella (Pichia) pastoris.

57 ed as a non-covalent dimer from secretion in Pichia pastoris (115 mg/l) and was a potent inhibitor of

58 Mutant enzymes were expressed in Pichia pastoris, a methylotrophic yeast strain, and thei

59 ; EC1.6.6.1) were cytosolically expressed in Pichia pastoris, a methylotrophic yeast, using spinach (

60 CPTI and CPTII were separately expressed in Pichia pastoris, a yeast with no endogenous CPT activity

61 When Pichia pastoris adapts from methanol to glucose growth,

62 Expression of an intronless ckx1 in Pichia pastoris allowed production of large amounts of r

63 Recombinant Pichia pastoris AMA1-FVO (PpAMA1-FVO) and PpAMA1-3D7 are

64 bacter globiformis amine oxidase (AGAO), and Pichia pastoris amine oxidase (PPLO) have been examined.

65 gC2 and gD2 were produced in glycoengineered Pichia pastoris and administered intramuscularly as a bi

66 The proteins were expressed in Pichia pastoris and adsorbed on Alhydrogel.

67 1-15 domains were expressed in high yield in Pichia pastoris and baculovirus, respectively.

68 In this report, we described two systems (Pichia pastoris and baculovirus/Sf9 cells) for expressio

69 eport that MS is localized to the nucleus of Pichia pastoris and Candida albicans but is cytoplasmic

70 d N- and O-linked mannosylation in the yeast Pichia pastoris and compared them to their unglycosylate

71 is rat Kv1.2 which has been overexpressed in Pichia pastoris and crystallised.

72 tide, SDF-2, are conserved between the yeast Pichia pastoris and D. discoideum.

73 1 and -DBL5 recombinant proteins produced in Pichia pastoris and developed a panel of seven chondroit

74 from barley aleurone tissue was expressed in Pichia pastoris and Escherichia coli.

75 an in vitro cell-free ER-budding assay using Pichia pastoris and followed two endogenous PMPs, Pex11p

76 e redox enzyme was recombinantly produced in Pichia pastoris and homogeneously purified, and its gluc

77 d Rlm7, the AvrLm4-7 protein was produced in Pichia pastoris and its crystal structure was determined

78 GSA12 in Pichia pastoris and its Saccharomyces cerevisiae counter

79 tic properties of VKORC1L1 when expressed in Pichia pastoris and more particularly its susceptibility

80 and the former is additionally conserved in Pichia pastoris and Paracoccus denitrificans, suggesting

81 7Y-hGALE proteins were also overexpressed in Pichia pastoris and purified for analysis.

82 d Cys-less P-glycoproteins were expressed in Pichia pastoris and purified in high yield in detergent-

83 d this aquaporin in the methylotrophic yeast Pichia pastoris and purified the hexahistidine-tagged pr

84 ant human ABCG5 and ABCG8 genes in the yeast Pichia pastoris and purified the proteins to near homoge

85 Human MATN-1 was cloned and expressed in Pichia pastoris and purified to homogeneity.

86 .1) was produced in the methylotrophic yeast Pichia pastoris and purified to near-electrophoretic hom

87 d multiple Bla g 2 mutants were expressed in Pichia pastoris and purified.

88 a-amylase expressed in amylolytic strains of Pichia pastoris and Saccharomyces cerevisiae.

89 rexpressed the human cytosolic ISCS in yeast Pichia pastoris and showed that the cytosolic form of IS

90 t was expressed in both Escherichia coli and Pichia pastoris and shown to be active.

91 x Arabidopsis GT31 members were expressed in Pichia pastoris and tested for enzyme activity.

92 oduced in glycoengineered lines of the yeast Pichia pastoris and that antibody-mediated effector func

93 WCI5 and WCI2 were expressed in Pichia pastoris and the recombinant proteins were assaye

94 hesis, the candidate genes were expressed in Pichia pastoris and their activities measured with the b

95 Recombinant thaumatin was expressed in Pichia pastoris and through a co-expression strategy wit

96 Here, we expressed recombinant hSMVT in Pichia pastoris and used affinity chromatography to puri

97 S-7 cells and the methylotropic yeast strain Pichia pastoris and was shown capable of penetrating int

98 ing peptides were expressed recombinantly in Pichia pastoris and were tested for their ability to bin

99 of human fibrinogen were expressed in yeast (Pichia pastoris) and characterized as to their cross-lin

100 s of 27,653 Daltons, was expressed in yeast (Pichia pastoris) and purified by anion exchange column c

101 es were expressed in Escherichia coli and in Pichia pastoris, and analyzed for monoclonal antibody an

102 n domain 11, expressed these mutant forms in Pichia pastoris, and determined binding kinetics with hu

103 ic human cysteine protease, was expressed in Pichia pastoris, and its physicokinetic properties were

104 Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, and mammalian cell lines.

105 eu, or Trp, the mutant proteins expressed in Pichia pastoris, and purified to homogeneity.

106 he polypeptide was recombinantly produced in Pichia pastoris, and the three-dimensional structure was

107 genesis-defective (pex) mutants of the yeast Pichia pastoris, AOX fails to assemble into active octam

108 thylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choi

109 and a deglycosylated form, both expressed in Pichia pastoris, are investigated and compared as biocat

110 ast, especially Saccharomyces cerevisiae and Pichia pastoris, are major hosts employed in the express

111 The PKCI-1 protein was expressed in Pichia pastoris as a dimer of two 13.7-kDa polypeptides.

112 loned by RACE-PCR and expressed in the yeast Pichia pastoris as a secreted enzyme.

113 Our results demonstrate the utility of using Pichia pastoris as an efficient eukaryotic host to expre

114 We used the methylotrophic yeast Pichia pastoris as an expression host to produce a large

115 ry and expressed in the methylotrophic yeast Pichia pastoris at a secretion yield of approximately 10

116 lic forms of AMA1 that were both produced in Pichia pastoris at a sufficient economy of scale to be u

117 termined by autophagy receptors, such as the Pichia pastoris autophagy-related protein 30 (Atg30), wh

118 When expressed in Pichia pastoris, AxlA had activity comparable to the nat

119 and pH 8.0 was purified from the engineered Pichia pastoris broth to homogeneity by anion exchange c

120 unaffected in most pex mutants of the yeast Pichia pastoris but is severely reduced in pex4 and pex2

121 lization pathway in the methylotrophic yeast Pichia pastoris by binding to Mxr1p response elements (M

122 of a form of P. falciparum AMA1, produced in Pichia pastoris, by vaccinating Aotus vociferans monkeys

123 romyces cerevisiae, the methylotrophic yeast Pichia pastoris can assimilate amino acids as the sole s

124 peroxisomal matrix, respectively, in living Pichia pastoris cells and followed by fluorescence micro

125 binant staphylokinase (SakSTAR) expressed in Pichia pastoris cells have been determined.

126 G/E56D/L72Y], was generated and expressed in Pichia pastoris cells in yields exceeding 100 mg/liter.

127 aE chain, was expressed in and purified from Pichia pastoris cells.

128 The variants were expressed in Pichia pastoris cells.

129 GT43-4, TaGT47-13, TaGT75-3, and TaGT75-4 in Pichia pastoris confirmed that these proteins form a com

130 The budding yeast Pichia pastoris contains discrete tER sites and is, ther

131 The budding yeast Pichia pastoris contains ordered Golgi stacks next to di

132 Purified recombinant PHACS, expressed in Pichia pastoris, contains bound pyridoxal-5'-phosphate (

133 study of expression of the RK gene in yeast (Pichia pastoris), COS-1 cells and in an HEK293 stable ce

134 go selection.We have identified Cvt9 and its Pichia pastoris counterpart Gsa9.

135 enicity of the natural allergen, whereas the Pichia pastoris-derived glycosylation does not.

136 from the AOX1 (alcohol oxidase) promoter of Pichia pastoris, displacing the chromosomal AOX1 gene.

137 thod is demonstrated on a flux experiment of Pichia pastoris employing two different tracers, i.e., 1

138 The recombinant enzyme expressed in Pichia pastoris established a 1.3:1 equilibrium between

139 otein obtained by heterologous expression in Pichia pastoris exhibited greater XET activity against x

140 All three Na-GSTs, when expressed in Pichia pastoris, exhibited low lipid peroxidase and glut

141 a streamlined workflow for the generation of Pichia pastoris expression strains, reducing the timelin

142 eine-rich domain of PyP140/RON4 by using the Pichia pastoris expression system and characterized the

143 alone or domain 9 alone were expressed in a Pichia pastoris expression system and tested for their a

144 erologous expression of this cDNA clone in a Pichia pastoris expression system led to the secretion i

145 The Pichia pastoris expression system offers economy, ease o

146 To investigate this hypothesis, we used a Pichia pastoris expression system to produce large amoun

147 binant human MD-2 (rhMD-2) was produced in a Pichia pastoris expression system, and the interaction b

148 Using the Pichia pastoris expression system, we show that cleavage

149 engineering of the DNA encoding VCP into the Pichia pastoris expression system, were used to localize

150 , were produced using Escherichia coli and a Pichia pastoris expression system.

151 ase after its heterologous expression in the Pichia pastoris expression system.

152 Active NR protein was produced in a Pichia pastoris expression system.

153 ession by small-scale fermentation using the Pichia pastoris expression system.

154 nt maize chitinase, rChiA, was purified from Pichia pastoris extracellular medium by differential pre

155 ernal standardization by fully (13)C labeled Pichia pastoris extracts enabled absolute quantification

156 is exemplified using unclarified broth from Pichia pastoris fermentation as feedstock.

157 in product (LeMir) was produced in the yeast Pichia pastoris for generation of antibodies.

158 CS proteins expressed in Escherichia coli or Pichia pastoris for their ability to induce immunity and

159 we report that, when expressed in the yeast Pichia pastoris, full-length ataxin-3 enabled almost nor

160 However, FBP1 when expressed in Pichia pastoris generated H2O2 using cysteine at pH 7.2,

161 coprotein (Pgp; mouse MDR3) was expressed in Pichia pastoris, grown in fermentor culture, and purifie

162 Recombinant EhCP5 expressed in Pichia pastoris had kinetic properties similar to those

163 lasminogen (amino acids 93-470) expressed in Pichia pastoris had physical properties (molecular size,

164 The methylotrophic yeast, Pichia pastoris, has been genetically engineered to prod

165 Because yeasts such as Pichia pastoris have been shown to O-glycosylate some pr

166 man bile salt-stimulated lipase expressed in Pichia pastoris, hen ovalbumin, bovine fetuin, bovine th

167 Finally, we demonstrate that the Pichia pastoris homologue Gsa7p that is required for per

168 protein expressed in the methylotropic yeast Pichia pastoris indicate that it catalyzes the 4-epimeri

169 lypeptide ABC transporter TAPL, expressed in Pichia pastoris, into lipid vesicles (liposomes) and per

170 Pichia pastoris is a methylotrophic yeast that has been

171 The methylotrophic yeast Pichia pastoris is a popular host for the production of

172 Pichia pastoris is a simple and powerful expression plat

173 Pichia pastoris is a yeast capable of expressing large a

174 The pas2 mutant of the methylotrophic yeast Pichia pastoris is characterized by a deficiency in pero

175 r methanol assimilation are synthesized when Pichia pastoris is grown in methanol.

176 nd NOP-1 protein heterologously expressed in Pichia pastoris is labeled by using all-trans [3H]retina

177 The yeast Pichia pastoris is used extensively as the host cell for

178 A. fumigatus Cu,Zn SOD has been expressed in Pichia pastoris, is enzymatically active, and has bioche

179 a water transporting aquaporin of the yeast Pichia pastoris, is suggested to be gated by chemo-mecha

180 (AOXI) promoter of the methylotrophic yeast, Pichia pastoris, is used widely for the production of re

181 pecies were synthesized in the yeast species Pichia pastoris: K195M, K199M, F211V, W214L, R218M, R222

182 phytochrome cDNA in the methylotrophic yeast Pichia pastoris led to time-dependent formation of photo

183 (EPAO), Pisum sativum amine oxidase (PSAO), Pichia pastoris lysyl oxidase (PPLO), bovine plasma amin

184 , Escherichia coli amine oxidase (ECAO), and Pichia pastoris lysyl oxidase (PPLO).

185 uman pancreatic alpha-amylase, concanavalin, Pichia pastoris lysyl oxidase, and Klebsiella pneumoniae

186 We isolated a Pichia pastoris mutant that was unable to grow on the pe

187 ressed this gene heterologously in the yeast Pichia pastoris, obtaining a relatively high yield of 2.

188 Growth of the yeast Pichia pastoris on methanol induces the expression of ge

189 ood yields cultivating the heterologous host Pichia pastoris on the 5L bioreactor scale (reUmChlE; 45

190 uffy binding-like (DBL) domains expressed in Pichia pastoris or var2csa plasmid DNA and sera were scr

191 the interaction between Pex19p and all known Pichia pastoris Pex proteins by the yeast two-hybrid ass

192 mport, we examined the behavior of PMPs in a Pichia pastoris pex17 mutant.

193 Pichia pastoris PEX17 was cloned by complementation of a

194 e report the cloning and characterization of Pichia pastoris PEX19 by complementation of a peroxisome

195 We show that Pichia pastoris Pex8p (PpPex8p) enters the peroxisome ma

196 The Pichia pastoris pexophagy receptor Atg30 is recruited to

197 hains was tested with human LAL expressed in Pichia pastoris (phLAL) and CHO cells (chLAL), respectiv

198 ng mannose terminated human LAL expressed in Pichia pastoris (phLAL), purified, and administered by t

199 Pichia pastoris (Pp) and Hansenula polymorpha (Hp) are m

200 Pichia pastoris (Pp) Pex8p, the only known intraperoxiso

201 been co-produced in the methylotrophic yeast Pichia pastoris (Pp).

202 s produced in both H. jecorina (HjCel3A) and Pichia pastoris (Pp-HjCel3A).

203 olded (EcCSP) or in the methylotrophic yeast Pichia pastoris (PpCSP) for structural analyses.

204 We show that the ScPex11p homologue in Pichia pastoris (PpPex11p) is phosphorylated at serine 1

205 Furthermore, we also demonstrated that Pichia pastoris produces XynCDBFV with higher catalytic

206 in of rat neural agrin (AgG3z8) expressed in Pichia pastoris promoted AChR clustering on surface of C

207 We show how a Pichia pastoris protein, PpAtg30, mediates peroxisome se

208 e effects of bile acids on ATP hydrolysis in Pichia pastoris purified ABCG5/G8 and found that they st

209 alpha2beta1, alpha2beta2, and alpha2beta3 in Pichia pastoris, purified the complexes, and compared th

210 d recombinant PI8 in the methylotropic yeast Pichia pastoris, purified the inhibitor to homogeneity,

211 ssed different variants of MDR3 in the yeast Pichia pastoris, purified the proteins via tandem affini

212 se mutation in a cysteine protease, G79E, in Pichia pastoris resulted in an unstable precursor protei

213 ologous expression of this gene in the yeast Pichia pastoris resulted in the production of a beta-1,4

214 n was expressed recombinantly in E. coli and Pichia pastoris, resulting in unglycosylated and mannosy

215 11GlcNAc species from invertase expressed in Pichia pastoris showed three and five peak fractions, re

216 P2, sLRP3, and sLRP4) have been expressed in Pichia pastoris SMD1168 with constitutive coexpression o

217 inant Tum-5 produced in Escherichia coli and Pichia Pastoris specifically inhibited proliferation and

218 In Komagataella phaffii (formerly called Pichia pastoris) specifically, the indirect traffic of P

219 ructosidase (BfrA) secreted by a recombinant Pichia pastoris strain was optimally immobilised on Glyo

220 oped a platform using genetically engineered Pichia pastoris strains designed to secrete multiple pro

221 In the Pichia pastoris system, the protease domain was expresse

222 mutant) were overproduced recombinantly in a Pichia pastoris system, they displayed the dual inhibito

223 e domain (amino acids 724-1429) in the yeast Pichia pastoris that for the first time exhibits a compl

224 lus nidulans) and in a methylotrophic yeast (Pichia pastoris), the latter expression system producing

225 common with a glycosylated form expressed in Pichia pastoris, the [(15)N,(1)H]-correlation spectra of

226 In Pichia pastoris, the orientation of a 138-kb invertible

227 In Pichia pastoris, the peroxisomal targeting signal 2 (PTS

228 in and expressed in the methylotrophic yeast Pichia pastoris to obtain a post-translationally modifie

229 ed and expressed in the methylotrophic yeast Pichia pastoris to probe for the proposed phosphatidylch

230 Here, we established an expression system in Pichia pastoris to recombinantly produce and purify Cx43

231 on of the glycosylation pathway in the yeast Pichia pastoris to secrete a human glycoprotein with uni

232 xpanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with full

233 AT2) heterodimers overexpressed in the yeast Pichia pastoris, together with docking analysis and cros

234 Here we successfully generated a Pichia pastoris transformant expressing and secreting ap

235 almitoyltransferase I (L-CPT I) expressed in Pichia pastoris, two contiguous discrete sequences withi

236 into the genome of the methylotrophic yeast Pichia pastoris under the control of an AOX promoter and

237 s expressed as a secreted soluble protein in Pichia pastoris under the regulation of alcohol oxidase

238 s nivalis agglutinin; GNA) were expressed in Pichia pastoris using native signal peptides, or the Sac

239 cts were expressed in a methyltrophic yeast, Pichia pastoris, using the alpha-mating factor secretion

240 er liter levels in the methylotrophic yeast, Pichia pastoris, using the methanol oxidase promoter.

241 s were observed against both rh-Endo and the Pichia pastoris vector, but no allergic reactions were o

242 The istk gene was expressed in Pichia pastoris vectors.

243 xpression system in the methylotrophic yeast Pichia pastoris was developed.

244 e recombinant protein expressed in the yeast Pichia pastoris was found to have activity against the i

245 The secretory pathway of Pichia pastoris was genetically re-engineered to perform

246 herapy, an Fv-p53 fusion protein produced in Pichia pastoris was tested on CT26.CL25 colon cancer cel

247 In-cell NMR in the yeast Pichia pastoris was used to study the influence of metab

248 A binding domain [BoNT/A(Hc)], expressed in Pichia pastoris, was developed as a vaccine candidate fo

249 me of 2964 base pairs and, when expressed in Pichia pastoris, was found to encode an enzyme that coul

250 f human recombinant cathepsin F, produced in Pichia pastoris, was processed to its active mature form

251 ress this, we expressed human CFH mutants in Pichia pastoris We found that recombinant I62-CFH (prote

252 ion assays of BdCSLF6 expressed in the yeast Pichia pastoris, we also demonstrate that the catalytic

253 e isolation of peroxisomal import mutants in Pichia pastoris, we have isolated a mutant (pex7) that i

254 e full-length enzyme (expressed in the yeast Pichia pastoris) were quantified.

255 e this idea, we examined two budding yeasts: Pichia pastoris, which has coherent Golgi stacks, and Sa

256 ACC synthase is now expressed in Pichia pastoris with an improved yield of 10 mg/L.

257 m freezing damage, we transformed the yeast, Pichia pastoris, with an inducible DEA1 construct.

258 ed in overexpressing PfCRT to high levels in Pichia pastoris yeast by synthesizing a codon-optimized

259 uced in single Escherichia coli bacteria and Pichia pastoris yeast cell in the current study.

260 sma membrane of Saccharomyces cerevisiae and Pichia pastoris yeast.

261 hancer, we expressed recombinant alpha2AP in Pichia pastoris yeast.

262 roteins from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic ch

263 Chy1 was expressed in Pichia pastoris yielding an enzyme with a chymotrypsin s