ライフサイエンスコーパス: PtdIns 4-kinase

1 PtdIns 4-kinase activity is conserved from yeast to high

2 , namely the Sec14p homolog PstB2p/Pdr17p; a PtdIns 4-kinase, Stt4p; and a C2 domain of Psd2p.

3 Overexpression of Pik1p, a PtdIns 4-kinase, suppressed the sec14-1 meiosis and spor

4 Specificity for CD151 and PtdIns 4-kinase association resided in the extracellular

5 5-kinase Mss4 (but not the Golgi-associated PtdIns 4-kinase Pik1).

6 s function of the plasma membrane-associated PtdIns 4-kinase Stt4 and the plasma membrane-associated

7 rylation of phosphatidylinositol (PtdIns) by PtdIns 4-kinases is the first step in the synthesis of p

8 he PH domain in the insect cells compromised PtdIns 4-kinase activity and caused mislocalization of t

9 We have recently identified a cytosolic PtdIns 4-kinase (cPI4K) in the bovine adrenal cortex tha

10 that we have cloned the previously described PtdIns 4-kinase that is responsible for regulating the s

11 pressing PIK1, encoding one of two essential PtdIns 4-kinases.

12 e cloning of a novel, ubiquitously expressed PtdIns 4-kinase (PI4Kbeta).

13 Here we demonstrate a role for PtdIns 4-kinases and PtdIns4P 5-kinases in selective and

14 OLPH3 family proteins are effectors of Golgi PtdIns 4-kinases.

15 Recently we isolated a distinct human PtdIns 4-kinase gene, named PI4Kbeta, that encodes an en

16 hese isoforms accounts for the major type II PtdIns 4-kinase activity detected in the lysosomes and p

17 ties to those of the membrane-bound, type II PtdIns 4-kinase activity of the adrenal cortex and the t

18 inase, and neither compound affected type II PtdIns 4-kinase at concentrations that inhibited cPI4K.

19 led that mammalian cells contain two type II PtdIns 4-kinase isoforms, now termed PI4KIIalpha and PI4

20 ound, Ca2+- and adenosine-sensitive, type II PtdIns 4-kinase.

21 indicated that type III rather than type II PtdIns 4-kinases are responsible for the maintenance of

22 The type III PtdIns 4-kinase prepared from bovine brain exhibited sim

23 the WT-sensitive adrenal cPI4K as a type III PtdIns 4-kinase that is distinct from the tightly membra

24 pport the idea that the Arabidopsis type III PtdIns 4-kinases are responsible for distinct phosphoino

25 and specific antibodies recognize a 110-kDa PtdIns 4-kinase in cell lysates.

26 of associated phosphatidylinositol-4-kinase (PtdIns 4-kinase) activity, most of which was removed upo

27 Phosphatidylinositol 4-kinases (PtdIns 4-kinases or PI4Ks) are at the apex of the phosph

28 that is distinguished from previously known PtdIns 4-kinases by its sensitivity to the PtdIns 3-kina

29 and characterization of the first mammalian PtdIns 4-kinase named PI4Kalpha.

30 Multiple isoforms of mammalian PtdIns 4-kinase have been purified, and the activities h

31 sed on enzymatic differences, two classes of PtdIns 4-kinase have been distinguished termed Types II

32 hila cells resulted in a severe reduction of PtdIns 4-kinase activity, suggesting the in vivo importa

33 and Pik1p act as the major, if not the only, PtdIns 4-kinases in yeast and produce distinct pools of

34 specific because overexpression of the other PtdIns 4-kinase gene (STT4) or a PtdIns 3-kinase gene (V

35 Phosphatidylinositol (PtdIns) 4-kinase catalyzes the synthesis of PtdIns-4-P,

36 Phosphatidylinositol (PtdIns) 4-kinases catalyze the conversion of PtdIns to P

37 Phosphatidylinositol (PtdIns) 4-kinases catalyze the synthesis of PtdIns-4-P,

38 two genes that encode phosphatidylinositol (PtdIns) 4-kinases, STT4 and PIK1.

39 e Arabidopsis type III phosphatidylinositol (PtdIns) 4-kinases, AtPI4Kalpha1 and AtPI4Kbeta1, in Spod

40 (PtdIns) 4-phosphate (PtdIns4P) by the Pik1 PtdIns 4-kinase and that modulation of the levels and ce

41 allele and one of two temperature-sensitive PtdIns 4-kinase genes, stt4(ts) or pik1(ts), we have dem

42 n sac1 mutant cells is generated by the Stt4 PtdIns 4-kinase, and not Pik1p.

43 the action of the Pik1p, but not the Stt4p, PtdIns 4-kinase.

44 The PtdIns 4-kinase Pik1 is involved in Atg9 trafficking thr

45 In Saccharomyces cerevisiae, the PtdIns 4-kinase Stt4 catalyzes the synthesis of PtdIns4P

46 Golgi targeting requires the activity of the PtdIns 4-kinase Pik1p but not phosphorylation of PtdIns