ライフサイエンスコーパス: Pyrococcus furiosus

1 lloproteins from an exemplary microorganism (Pyrococcus furiosus).

2 (NfnI) from the hyperthermophillic archaeon Pyrococcus furiosus.

3 nditions from the hyperthermophilic archaeon Pyrococcus furiosus.

4 NA circles in the hyperthermophilic archaeon Pyrococcus furiosus.

5 ucture of the full-length RAD51 homolog from Pyrococcus furiosus.

6 purified from the hyperthermophilic archaeon Pyrococcus furiosus.

7 e from the hyperthermophilic archaebacterium Pyrococcus furiosus.

8 to 1.2 A from the hyperthermophilic archaeon Pyrococcus furiosus.

9 frame (ORFs) from the genome of the archaeon Pyrococcus furiosus.

10 P-free Rad50 catalytic domain (Rad50cd) from Pyrococcus furiosus.

11 n compared to that from the hyperthermophile Pyrococcus furiosus.

12 ba histolytica and from the archaebacterium, Pyrococcus furiosus.

13 sing Bacillus subtilis, Escherichia coli and Pyrococcus furiosus.

14 purified from the hyperthermophilic archaeon Pyrococcus furiosus.

15 lfovibrio gigas, Desulfovibrio vulgaris, and Pyrococcus furiosus.

16 cohol dehydrogenase (AdhA) into the archaeon Pyrococcus furiosus.

17 ss spectrometer to profile the proteome from Pyrococcus furiosus.

18 bus solfataricus, Sulfolobus islandicus, and Pyrococcus furiosus.

19 to those of V-type ATPases, namely that from Pyrococcus furiosus.

20 nosine, from the hyperthermophilic archaeon, Pyrococcus furiosus.

21 ssed, using the family-B DNA polymerase from Pyrococcus furiosus.

22 -keto reductase activity native to AdhD from Pyrococcus furiosus.

23 und low-spin FeIII forms of the 1Fe SOR from Pyrococcus furiosus.

24 d a surrogate protein system using RadA from Pyrococcus furiosus.

25 utagenesis in the hyperthermophilic archaeon Pyrococcus furiosus.

26 sing the thermostable protease isolated from Pyrococcus furiosus.

27 ophilum (optimal growth at 55 degrees C) and Pyrococcus furiosus (100 degrees C) are homo-dimeric enz

28 e archaeal transcriptional regulator SurR of Pyrococcus furiosus, a hyperthermophilic anaerobe.

29 We have microinjected Box C/D RNAs from Pyrococcus furiosus, a hyperthermophilic archaeon, into

30 l extracts of the hyperthermophilic archaeon Pyrococcus furiosus: a beta-glucosidase, corresponding t

31 e we show that purified Mre11 and Rad50 from Pyrococcus furiosus act cooperatively with HerA and NurA

32 found that the hyperthermophilic archaeaon, Pyrococcus furiosus, actively incorporates DNA fragments

33 This is not the case with Pyrococcus furiosus, an archaeon that grows optimally ne

34 urified from the hyperthermophilic archaeon, Pyrococcus furiosus, an organism that grows optimally at

35 50 homologues from the thermophilic archaeon Pyrococcus furiosus and demonstrate that the two protein

36 and human clamp loaders, and the two protein Pyrococcus furiosus and Methanobacterium thermoautotroph

37 Divergence of the hyperthermophilic Archaea, Pyrococcus furiosus and Pyrococcus horikoshii, was asses

38 Pyrococcus furiosus and Pyrococcus woesei grow optimally

39 mate dehydrogenase from the hyperthermophile Pyrococcus furiosus and the comparison of this structure

40 bredoxin from the hyperthermophilic archaeon Pyrococcus furiosus and the mesophilic bacterium Clostri

41 te dehydrogenases from the hyperthermophiles Pyrococcus furiosus and Thermococcus litoralis whose opt

42 In the Euryarchaeota species Pyrococcus furiosus and Thermococcus litoralis, phosphog

43 egion between two hyperthermophilic Archaea, Pyrococcus furiosus and Thermococcus litoralis.

44 have applied directed evolution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate

45 cale applications on Caenorhabditis elegans, Pyrococcus furiosus and three cyanobacterial genomes are

46 se and two proteins of unknown function from Pyrococcus furiosus and Vibrio parahaemolyticus.

47 l element for the hyperthermophilic archaeon Pyrococcus furiosus, and many of its iron-containing enz

48 hia coli, Artemisia tridentata (sage brush), Pyrococcus furiosus, and Methanobacter thermautotrophicu

49 cus litoralis, Thermococcus sp. strain ES-1, Pyrococcus furiosus, and Pyrococcus sp. strain ES-4 cont

50 he metabolite was isolated from the organism Pyrococcus furiosus, and structurally characterized thro

51 cryoEM), we have solved the structure of the Pyrococcus furiosus archaellum filament at 4.2 A resolut

52 Pyrococcus horikoshii (PH1704) and PfpI from Pyrococcus furiosus are members of a class of intracellu

53 ironment, such as hyperthermophilic archaea (Pyrococcus furiosus), are significantly more compact and

54 stal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution.

55 protein-translocating channel SecYEbeta from Pyrococcus furiosus at 3.1-A resolution suggests a mecha

56 ation cofactor, S-adenosyl-L-methionine from Pyrococcus furiosus, at 2.7 A.

57 ulfolobus solfataricus N-terminal domain and Pyrococcus furiosus ATPase domain.

58 protein from the hyperthermophilic archaeon Pyrococcus furiosus belongs to the Lrp/AsnC family of tr

59 l activator, PF1088, which was identified in Pyrococcus furiosus by a bioinformatic approach.

60 systems, Cmr eliminates plasmid invaders in Pyrococcus furiosus by a mechanism that depends on trans

61 l extracts of the hyperthermophilic archaeon Pyrococcus furiosus by its ability to hydrolyze N-acetyl

62 the proteolytic, hyperthermophilic archaeon Pyrococcus furiosus by multistep chromatography.

63 dase A, but instead resembles neurolysin and Pyrococcus furiosus carboxypeptidase--zinc metallopeptid

64 Here we identified Pyrococcus furiosus Cas6 as a novel endoribonuclease tha

65 CESI-top-down MS analysis of Pyrococcus furiosus cell lysate identified 134 proteins

66 Using reconstituted Pyrococcus furiosus Cmr complexes, we found that each of

67 from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9

68 the proteolytic, hyperthermophilic archaeon Pyrococcus furiosus contain high specific activity (11 U

69 annotation of the hyperthermophilic archaeon Pyrococcus furiosus contained 2,065 open reading frames

70 The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterot

71 ve shown that the hyperthermophilic archaeon Pyrococcus furiosus contains four distinct cytoplasmic 2

72 (7.5 kDa) of the hyperthermophilic archaeon, Pyrococcus furiosus, contains a single [4Fe-4S]1+,2+ clu

73 The thermostable DNA polymerase derived from Pyrococcus furiosus designated Pfu has the highest fidel

74 The metal-ion-dependent enzyme from Pyrococcus furiosus DSM 3638 showed a relatively high de

75 icus P2), and an anaerobic hyperthermophile (Pyrococcus furiosus DSM 3638).

76 The genome of the hyperthermophile archaeon Pyrococcus furiosus encodes two transcription factor B (

77 information has been available only for the Pyrococcus furiosus enzyme (PfMre11), the conserved and

78 se extensions of stabilizing interactions in Pyrococcus furiosus Fd, however, lead to strong destabil

79 The crystal structure of Pyrococcus furiosus FEN-1, active-site metal ions, and m

80 Pyrococcus furiosus ferredoxin (Fd) contains a single [F

81 ication of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

82 the nature of the residue at position 14 in Pyrococcus furiosus ferredoxin is an important determina

83 e conformationally dynamically heterogeneous Pyrococcus furiosus ferredoxin with an intact disulfide

84 The hyperthermophilic archaeon Pyrococcus furiosus genome encodes three proteasome comp

85 in the AT-rich Methanococcus jannaschii and Pyrococcus furiosus genomes efficiently detects both kno

86 The anaerobic archaeon Pyrococcus furiosus grows by fermenting carbohydrates pr

87 The hyperthermophilic archaeon Pyrococcus furiosus grows optimally at 100 degrees C by

88 The archaeon Pyrococcus furiosus grows optimally at 100 degrees C by

89 The model archaeon Pyrococcus furiosus grows optimally near 100 degrees C o

90 Pyrococcus furiosus grows optimally near 100 degrees C u

91 ave recently shown that the hyperthermophile Pyrococcus furiosus has an extraordinarily high capacity

92 PF0610, a protein from the hyperthermophile Pyrococcus furiosus, has homologues only in other archae

93 s of SOR from the hyperthermophilic archaeon Pyrococcus furiosus have been determined in the oxidized

94 of the archaeal family-B DNA polymerase from Pyrococcus furiosus have been investigated, illuminating

95 protein from the hyperthermophilic archaeon Pyrococcus furiosus, have been determined in the native

96 r-2 gene from the hyperthermophilic archaeon Pyrococcus furiosus having homology to bacterial and euk

97 hyperthermophiles Pyrococcus horikoshii and Pyrococcus furiosus; however, the form(s) of sulfur that

98 e with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase.

99 The crystal structure of GalK from Pyrococcus furiosus in complex with MgADP and galactose

100 structure-specific 5' flap endonuclease from Pyrococcus furiosus in its complex with DNA.

101 determined the crystal structure of PGI from Pyrococcus furiosus in native form and in complex with t

102 Pyrococcus furiosus is a hyperthermophilic archaeon that

103 Pyrococcus furiosus is a hyperthermophilic archaeon whic

104 rredoxin from the hyperthermophilic archaeon Pyrococcus furiosus is a monomeric protein (7.5 kDa) tha

105 Pyrococcus furiosus is a strictly anaerobic archaeon (ar

106 The archaeon Pyrococcus furiosus is a strictly anaerobic heterotroph

107 nase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NADP(H)-dependent heterotetram

108 Rubredoxin from the hyperthermophile Pyrococcus furiosus is the most thermostable protein cha

109 irst characterized from the hyperthermophile Pyrococcus furiosus, it is unique to the archaeal order

110 Pyrococcus furiosus LrpA forms a homodimer mainly throug

111 tors: Ptr-H10, fusing the effector domain of Pyrococcus furiosus LrpA, and Ptr-H16, fusing the P. fur

112 We determined the crystal structure of the Pyrococcus furiosus MCM N-terminal domain hexamer bound

113 f the type-I (Escherichia coli) and type-II (Pyrococcus furiosus) MetAPs in the presence of the react

114 hermus thermophilus, Archaeoglobus fulgidus, Pyrococcus furiosus, Methanococcus jannaschii, and Metha

115 in DNA double-strand break repair, we report Pyrococcus furiosus Mre11 crystal structures, revealing

116 scattering (SAXS) and crystal structures of Pyrococcus furiosus Mre11 dimers bound to DNA with mutat

117 X-ray crystallography of Pyrococcus furiosus Mre11 indicates that an analogous mu

118 M N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in the hexameric

119 2025) is highly upregulated during growth of Pyrococcus furiosus on elemental sulfur (S(0)).

120 s) that have been annotated in the genome of Pyrococcus furiosus (optimal growth temperature, 100 deg

121 ng enzyme have been previously purified from Pyrococcus furiosus (optimum growth temperature, 100 deg

122 cluster in the D14C variant ferredoxin from Pyrococcus furiosus (Pf D14C Fd).

123 of the rubredoxins from the hyperthermophile Pyrococcus furiosus (Pf) and the mesophile Clostridium p

124 s of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Cl

125 se (POR) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) catalyzes the final oxidative s

126 xin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) have been characterized by (1)H

127 xin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) possesses several unique proper

128 distance to the [Fe(SCys)(4)] center in the Pyrococcus furiosus (Pf) Rd crystal structure compared t

129 Amide exchange rates were measured for Pyrococcus furiosus (Pf) rubredoxin substituted with eit

130 ine monophosphate dehydrogenase (IMPDH) from Pyrococcus furiosus (Pf), a hyperthermophillic archeon.

131 ter of the hyperthermophilic marine archaea, Pyrococcus furiosus (Pf), we have determined the locatio

132 in reduced and oxidized rubredoxin (Rd) from Pyrococcus furiosus (Pf).

133 The Argonaute of the archaeon Pyrococcus furiosus (PfAgo) belongs to a different branc

134 ferritin from the hyperthemophilic bacterium Pyrococcus furiosus (PfFt), have been used as models for

135 nding studies with a mutant of ferritin from Pyrococcus furiosus (PfFtn) in which self-assembly was a

136 cMetAP-I) and the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II) was investigated.

137 coli (EcMetAP-I) and the type II MetAP from Pyrococcus furiosus (PfMetAP-II).

138 eptidase from the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II; EC 3.4.11.18) has been

139 structures of apo- and holorubredoxins from Pyrococcus furiosus (PfRd) and Clostridium pasteurianum

140 rks in rubredoxins from the hyperthermophile Pyrococcus furiosus (PfRd), and its mesophilic analogue

141 luble [NiFe]-hydrogenase: hydrogenase I from Pyrococcus furiosus (PfSHI), which grows optimally near

142 the beta-subunit of tryptophan synthase from Pyrococcus furiosus (PfTrpB).

143 of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus ( Pfu) using conventional and parama

144 he thermostable family B DNA polymerase from Pyrococcus furiosus (Pfu Pol) contains sensitive determi

145 on the POP of the hyperthermophilic archaeon Pyrococcus furiosus (Pfu) 85 degrees C in both H(2)O and

146 P holoenzyme from the thermophilic archaeon Pyrococcus furiosus (Pfu) and furthered our understandin

147 f-replication (CSR), we evolved a version of Pyrococcus furiosus (Pfu) DNA polymerase that tolerates

148 Pyrococcus furiosus (Pfu) harbors three CRISPR-Cas immun

149 ng strategy to pinpoint the binding sites of Pyrococcus furiosus (Pfu) L7Ae on its cognate RNase P RN

150 ronounced unwinding of primer-templates with Pyrococcus furiosus (Pfu) polymerase-DNA complexes conta

151 We investigated Pyrococcus furiosus (Pfu) RNase P, an archaeal RNP that

152 urthermore, an N-terminal deletion mutant of Pyrococcus furiosus (Pfu) RPP29 that is defective in ass

153 plification utilized the DNA polymerase from Pyrococcus furiosus (Pfu) which, unlike Taq, does not in

154 ered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR

155 mutants of the family B DNA polymerase from Pyrococcus furiosus (Pfu-Pol), with superb performance i

156 A maltodextrin-binding protein from Pyrococcus furiosus (PfuMBP) has been overproduced in Es

157 maltose binding protein from the thermophile Pyrococcus furiosus (PfuMBP).

158 Pyrococcus furiosus phosphoglucose isomerase (PfPGI) is

159 Pyrococcus furiosus phosphoglucose isomerase (PfPGI), an

160 Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three prot

161 The hyperthermophilic archeon Pyrococcus furiosus produces an extracellular alpha-amyl

162 x cleaves an endogenous complementary RNA in Pyrococcus furiosus, providing direct in vivo evidence o

163 In Pyrococcus furiosus, psiRNAs occur in two size forms tha

164 Here we design structure-based mutations in Pyrococcus furiosus Rad50 to alter protein core plastici

165 Based on the recent Pyrococcus furiosus Rad51 structure, we have used homolo

166 ics of rubredoxins from the hyperthermophile Pyrococcus furiosus (RdPf) and the mesophile Clostridium

167 he small iron-sulfur protein rubredoxin from Pyrococcus furiosus (RdPf) at pH 2.

168 of operons in the hyperthermophilic archaeon Pyrococcus furiosus represents an important step to unde

169 obus fulgidus, Methanococcus jannaschii, and Pyrococcus furiosus, respectively.

170 the 2.3 A crystal structure of mre11-3 from Pyrococcus furiosus revealed an active site structure wi

171 re of the protein-protein complex comprising Pyrococcus furiosus RPP21 and RPP29.

172 on Zn(II)-, Ga(III)-, and Ge(IV)-substituted Pyrococcus furiosus rubredoxin demonstrate that the log

173 (Trp) solvation dynamics in water and in the Pyrococcus furiosus rubredoxin protein, including the na

174 static solvent-accessible amide hydrogens of Pyrococcus furiosus rubredoxin range from near the diffu

175 l-atom molecular-dynamics simulations of the Pyrococcus furiosus SecYE, which was determined to be in

176 identification and characterization of SurR, Pyrococcus furiosus sulphur (S(0)) response regulator.

177 perties of the oxidized and reduced forms of Pyrococcus furiosus superoxide reductase (SOR) as a func

178 ction at the mononuclear iron active site of Pyrococcus furiosus superoxide reductase (SOR) through t

179 he site of base exchange; truncated forms of Pyrococcus furiosus TGT retain their specificity for gua

180 ed a gene from the archaeal hyperthermophile Pyrococcus furiosus that reduces O(2)(-).

181 gen exchange results for the rubredoxin from Pyrococcus furiosus, the acidity of these amides was cal

182 etAP from E. coli and the type-II MetAP from Pyrococcus furiosus, the type-I MetAP can be selectively

183 the N-terminal DNA binding domain of Phr of Pyrococcus furiosus, these results suggest that HSR1 and

184 in from the hyperthermophilic archebacterium Pyrococcus furiosus to understand the unusual temperatur

185 cted 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30

186 (Topt = 37 degrees C) and hyperthermophilic Pyrococcus furiosus (Topt = 95 degrees C) were recorded

187 n initiation complexes have been formed with Pyrococcus furiosus transcription factors (TBP and TFB1)

188 cations with larger quantities (100 ng) of a Pyrococcus furiosus tryptic digest, but with mass-limite

189 The hyperthermophilic archaeon Pyrococcus furiosus uses carbohydrates as a carbon sourc

190 eductase from the hyperthermophilic anaerobe Pyrococcus furiosus uses electrons from reduced nicotina

191 r E17 to Q in the soluble hydrogenase I from Pyrococcus furiosus using site directed mutagenesis.

192 Pyrococcus furiosus utilizes starch and its degradation

193 gene rgy from the hyperthermophilic archaeon Pyrococcus furiosus was cloned and sequenced.

194 n from the hyperthermophilic archaebacterium Pyrococcus furiosus was examined by a hydrogen exchange

195 c activity in the hyperthermophilic archaeon Pyrococcus furiosus was found to be a homomultimer consi

196 cosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escher

197 ock protein (sHSP) from the hyperthermophile Pyrococcus furiosus was specifically induced at the leve

198 lucanase from the hyperthermophilic archaeon Pyrococcus furiosus, was cloned and expressed in Escheri

199 The hyperthermophilic archaeon, Pyrococcus furiosus, was grown on maltose near its optim

200 Herein, the model hyperthermophile, Pyrococcus furiosus, which grows optimally near 100 degr

201 hat confers on a microorganism (the archaeon Pyrococcus furiosus, which grows optimally on carbohydra

202 ned the structures of a PurP orthologue from Pyrococcus furiosus, which is functionally unclassified,

203 amily B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures approxim

204 tryptophan synthase beta-subunit (TrpB) from Pyrococcus furiosus, which mimics the activation afforde

205 l-II family DNA polymerase from the archaeon Pyrococcus furiosus with the aim of improving ddNTP util

206 gineered subunit of tryptophan synthase from Pyrococcus furiosus, yielding (2S,3S)-beta-methyltryptop