ライフサイエンスコーパス: R. capsulatus

1 Using a R. capsulatus genetic system, the cyt c1 mutants M183K a

2 All R. capsulatus cycJ mutants studied so far excrete copiou

3 An R. capsulatus gene responsible for long-chain acyl-homos

4 Surprisingly, an R. capsulatus strain with the fnrL gene deleted grows li

5 ast to wild-type strains, R. sphaeroides and R. capsulatus fnrL mutants do not synthesize the anaerob

6 nesis in some gram-negative bacteria such as R. capsulatus.

7 ment of these natural promoters activated by R. capsulatus RNAP/sigma70 indicated a preference for th

8 Thus, an additional barrier to activation by R. capsulatus NtrC exists, probably a lack of the proper

9 on of high-GC promoters or for activation by R. capsulatus NtrC.

10 and Ccl2 (a soluble, truncated form of Ccl2) R. capsulatus proteins, respectively.

11 y phenotypes upon overproduction of the CcmF-R. capsulatus CcmH (CcmF-CcmH(Rc)) couple in a growth me

12 osed to act as an apocytochrome c chaperone, R. capsulatus does not have the ability to produce holoc

13 The complemented R. capsulatus strain contains a defined mutation in the

14 findings therefore demonstrate that, during R. capsulatus growth on minimal medium, the requirement

15 sion in an E. coli plsC(Ts) mutant of either R. capsulatus plsC316 or olsA gene products supported gr

16 r detergent dispersed chromatophore-embedded R. capsulatus bc(1) complex, we demonstrated that while

17 e of -10 promoter mutants did not facilitate R. capsulatus NtrC activation of the nifA1 promoter by t

18 ase (RNAP) that contains the sigma70 factor (R. capsulatus RNAP/sigma70) was purified and characteriz

19 f a peptide designed to serve as a model for R. capsulatus apocytochrome c(2) have also been carried

20 y of either olsA or plsC316 was required for R. capsulatus growth under the conditions tested.

21 cific transcripts were detected in vitro for R. capsulatus cytochrome c2 (cycA) and fructose-inducibl

22 Thus, no additional factors from R. capsulatus are necessary for the recognition of high-

23 made with large and small subunit genes from R. capsulatus and R. sphaeroides, also supported the unr

24 e of this non-phosphorus membrane lipid from R. capsulatus.

25 obacter sphaeroides, the form I RubisCO from R. capsulatus is a member of the green-like group and cl

26 In R. capsulatus, CcmI-null mutants are unable to produce c

27 utants affected in cyt c oxidase activity in R. capsulatus led to the isolation of at least five clas

28 og was identified 127 bp upstream of acxA in R. capsulatus, but this activator lacked key features of

29 is not required for expression of acxABC in R. capsulatus.

30 oters (nifA1, nifA2, glnB, mopA and anfA) in R. capsulatus which are transcriptionally activated by N

31 an operon essential for cyt c biogenesis, in R. capsulatus, it is located immediately downstream from

32 complementing them revealed that ccoNOQP in R. capsulatus is not flanked by the oxygen response regu

33 tic heme-apocytochrome c ligation complex in R. capsulatus.

34 of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR

35 either plsC316 nor plsC3498 was essential in R. capsulatus.

36 Membrane topology of CcdA was established in R. capsulatus using ccdA:phoA and ccdA :lacZ gene fusion

37 nsing signals to enhance genetic exchange in R. capsulatus.

38 e subunits (Bchl and BchN) were expressed in R. capsulatus as S tag fusion proteins that facilitated

39 no active CcoN-CcoO subcomplex was found in R. capsulatus.

40 ggesting that there is only one cbbP gene in R. capsulatus and that this gene is cotranscribed with c

41 We demonstrate that the native HelX in R. capsulatus is tethered to the cytoplasmic membrane vi

42 ssembly but also regulates Cu homeostasis in R. capsulatus.

43 s, R. sphaeroides cyt cy can act at least in R. capsulatus as an electron carrier between the cyt bc1

44 enoid and bacteriochlorophyll metabolites in R. capsulatus.

45 The nifR3-ntrB-ntrC operon in R. capsulatus codes for the nitrogen-sensing two compone

46 olysin, or an endogenous activity present in R. capsulatus, cleaves the hinge region of the Fe-S subu

47 sion carrying the G488A mutation produced in R. capsulatus over 30-fold higher beta-galactosidase act

48 prelude to studies of cbb gene regulation in R. capsulatus, the nucleotide sequence of a 4,537-bp reg

49 bR responded to the same metabolic signal in R. capsulatus SBI/II and mutant strain backgrounds.

50 equired for glycerophospholipid synthesis in R. capsulatus, while olsA acts as an alternative AGPAT t

51 This finding demonstrates that in R. capsulatus the dithiol:disulfide oxidoreductases DsbA

52 wn to be Ps(+) Nadi(+), establishing that in R. capsulatus the inactivation of dsbA suppresses the c-

53 ory or photosynthetic energy transduction in R. capsulatus.

54 in respiratory electron transfer, unlike its R. capsulatus counterpart, Cyt cyRc.

55 Anaerobically in the light, R. capsulatus requires cytochrome bc1 and other c-type c

56 ted in the hinge region (positions 43-49) of R. capsulatus Fe-S subunit was not essential per se for

57 mediator ADP had no effect on the ability of R. capsulatus LPS to stimulate NO production but signifi

58 oteins either greatly reduced the ability of R. capsulatus to support growth or had little effect, re

59 The addition of R. capsulatus sigma(70) to the E. coli core RNA polymera

60 A comparative analysis of R. capsulatus and other alpha-proteobacterial promoters

61 on of the heme-Cu-containing subunit CcoN of R. capsulatus cbb(3)-Cox proceeds independently of that

62 epitope-tagged and functional derivative of R. capsulatus cyt c(y) was purified from intracytoplasmi

63 e that some OlsA enzymes, like the enzyme of R. capsulatus, are bifunctional and involved in both mem

64 s shown here that the ccl1 and ccl2 genes of R. capsulatus are required for the synthesis of all c-ty

65 e previously known cyt c biogenesis genes of R. capsulatus.

66 upports efficiently photosynthetic growth of R. capsulatus in the absence of cyt c2 because it can me

67 was able to support photosynthetic growth of R. capsulatus in the absence of the cyt c(y) and c2.

68 sm during anaerobic photosynthetic growth of R. capsulatus.

69 able to support the photosynthetic growth of R. capsulatus.

70 rved previously in a bc(1) complex mutant of R. capsulatus.

71 he DsbA-null and DsbB-null single mutants of R. capsulatus are Ps(+) and produce c-type cytochromes,

72 indicated that the cbbI and cbbII operons of R. capsulatus are within separate CbbR regulons.

73 ng the biogenesis of the cyt cbb3 oxidase of R. capsulatus.

74 recently proposed phylogenetic placement of R. capsulatus form I RubisCO.

75 ences between the RNAPs and the promoters of R. capsulatus and E. coli are discussed.

76 In specific mutant strains of R. capsulatus, expression of both the Calvin-Benson-Bass

77 To facilitate studies of R. capsulatus transcription, we cloned and overexpressed

78 wever, R. sphaeroides cyt cy, unlike that of R. capsulatus, is unable to function as an efficient ele

79 . sphaeroides are more complex than those of R. capsulatus.

80 s NtrC exists, probably a lack of the proper R. capsulatus NtrC-E. coli RNA polymerase (protein-prote

81 ls of in vitro transcription by the purified R. capsulatus RNAP/sigma70 enzyme.

82 mation and function showed that the purified R. capsulatus sigma N protein is distinct in activity co

83 uted in vitro to form an active, recombinant R. capsulatus RNA polymerase with properties mimicking t

84 It is proposed that RcNtrC recruits R. capsulatus sigma 70-RNA polymerase to the promoter th

85 the R. capsulatus bchM mutant via the strong R. capsulatus puc promoter was shown to support nearly w

86 ents confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotati

87 molecular genetic studies, we inferred that R. capsulatus CcmF, CcmH, and CcmI interact with each ot

88 The R. capsulatus enzyme is the smallest of the IPP isomeras

89 The R. capsulatus form I enzyme was found to be subject to a

90 The R. capsulatus HelX and Ccl2 proteins are predicted to fu

91 NtrC enhancer-binding protein activates the R. capsulatus housekeeping RNA polymerase but not the Es

92 but contact of certain promoter bases by the R. capsulatus sigma N protein and its response to core R

93 A cosmid carrying the R. capsulatus reg locus was capable of complementing an

94 In contrast, the R. capsulatus ccdA was homologous to the cyt c biogenesi

95 Depletion of manganese from the R. capsulatus growth medium resulted in inhibition of ac

96 expression of the Synechocystis gene in the R. capsulatus bchM mutant via the strong R. capsulatus p

97 bic growth on DMSO is not synthesized in the R. capsulatus fnrL mutant.

98 rol of the CBB pathway and regulation of the R. capsulatus cbb genes were studied by using a combinat

99 taI, resulted in decreased production of the R. capsulatus gene transfer agent, and gene transfer age

100 . autotrophicus and sequence analysis of the R. capsulatus genome and were found to be clustered in s

101 sm was used to evaluate the structure of the R. capsulatus protein and revealed differences in the te

102 protein that activates transcription of the R. capsulatus sigma 70 RNA polymerase, but does not acti

103 To test the -35 recognition pattern, the R. capsulatus nifA1 promoter, which exhibits only three

104 facile genetic manipulation, we purified the R. capsulatus form I enzyme and determined its basic kin

105 These results suggest that the R. capsulatus alpha subunit is not important for RcNtrC

106 Therefore, the R. capsulatus NtrB and NtrC proteins form a two-componen

107 constraints on how RcNtrC interacts with the R. capsulatus RNA polymerase.

108 CO-containing bacterium and a predecessor to R. capsulatus.

109 A kinetic isotope study of the wild-type R. capsulatus enzyme indicates that, as previously deter

110 DNase I footprint analyses, using R. capsulatus RegA*, a constitutively active mutant vers

111 We utilized R. capsulatus:E. coli hybrid RNA polymerases assembled i

112 nisms that govern the diverse means by which R. capsulatus maintains redox poise during photoheterotr

113 These results are consistent with R. capsulatus cytochrome c2 stabilizing the complex thro