ライフサイエンスコーパス: R. etli

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1 hesized from B and D-1, respectively, by the R. etli 3-O-deacylase.

2 As in R. etli, a 4-carbon fatty acid, beta-hydroxybutyrate, is

3 size of nodules formed upon inoculation with R. etli and affected the progression of infection thread

4 hat is phosphorylated at positions 1 and 4', R. etli and R. leguminosarum lipid A consists of a mixtu

5 present in membranes of R. leguminosarum and R. etli but not in S. meliloti or Escherichia coli.

6 trate that membranes of R. leguminosarum and R. etli can convert B to D-1 in a reaction that requires

7 mpared with the LPS from laboratory-cultured R. etli CE3 and from cultures grown in the presence of a

8 plasmids that shared a 7.8-kb stretch of the R. etli CE3 lps genetic region alpha, even though this s

9 ferases, and a model for biosynthesis of the R. etli CE3 O antigen was proposed.

10 created a pagL(-) mutant strain derived from R. etli CE3.

11 3841 genomic DNA library in the host strain R. etli CE3.

12 abundance of galacturonosyl residues in the R. etli core might serve as a suitable functional replac

13 A putative pagL gene was identified in the R. etli genome sequence.

14 ndings, we propose a biosynthetic scheme for R. etli lipid A in which B is generated first by a varia

15 In addition, R. etli lipid A is reported to lack phosphate and acylox

16 All R. etli lipid A species lack phosphate groups.

17 However, the proposed R. etli lipid A structure is inconsistent with the abili

18 The R. etli lipid A subtypes each contain an unusual acyloxy

19 d A retains the unusual acylation pattern of R. etli lipid A, including the presence of a distal, ami

20 residue found in species D-1, D-2, and E of R. etli lipid A.

21 n to lacking both heptose and phosphate, the R. etli LPS core region differs substantially from the t

22 suggest that the unusual lipid A species of R. etli might be essential during symbiosis with legumin

23 bodies fail to recognize previously isolated R. etli mutant strain CE367, even in the absence of such

24 R. etli mutants lacking only the methylated terminal fuc

25 Two other R. etli mutants that had one-half or less of the normal

26 gh the characterization of the LPSs from two R. etli mutants.

27 f the carboxyltransferase domain reveal that R. etli PC occupies a symmetrical conformation in the ab

28 The unusual structural features shared with R. etli/R. leguminosarum lipid A may be essential for sy

29 ly influences expression of diverse genes in R. etli, some of which affect the cell surface and nodul

30 , we mutagenized a rosR mutant derivative of R. etli strain CE3 with a mini-Tn5 that contains a promo

31 enzyme(s) can be demonstrated in extracts of R. etli that convert (14)C-labeled B to D-1.

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