1 R. eutropha phaC PHA synthase and phaP phasin gene repla
2 R. eutropha strains with phaR, phaC, and/or phaP deletio
3 binant PhaP, antibodies against PhaP, and
an R. eutropha phaP deletion strain have been generated for
4 tributed to the beta-ketothiolase encoded
by R. eutropha phbA.
5 ion of candidate PHB depolymerase genes
from R. eutropha, namely, phaZ2 and phaZ3, and their characte
6 of conserved cysteine residues in HypD
from R. eutropha was investigated.
7 bktB, which encodes a beta-ketothiolase
from R. eutropha that is capable of forming beta-ketovaleryl-
8 f PhaZ2 and PhaZ3 was examined by
generating R. eutropha H16 deletion strains (Delta phaZ1, Delta pha
9 tion of HoxR protein from its original
host,
R. eutropha, revealed only low protein amounts.
10 is the limiting step in PHB biosynthesis
in R. eutropha H16 during nitrogen-limited growth on fructo
11 ding these enzymatic steps was discovered
in R. eutropha and has been well studied.
12 ent physiological and ecological function
in R. eutropha.
13 lglycerol and fatty acid catabolism genes
in R. eutropha.
14 tify two new candidate depolymerase genes
in R. eutropha: phaZ2 and phaZ3.
15 ntly created genus Ralstonia, which
includes R. eutropha, R. pickettii, and R. solanacearum.
16 s permit uptake of radiolabeled sucrose
into R. eutropha cells.
17 Moreover, engineering
of R. eutropha enabled production of the fusel alcohol isop
18 compare whole-cell gene expression levels
of R. eutropha H16 during growth and polyhydroxyalkanoate p
19 Analysis of a phcA-null mutant
of R. eutropha showed that phcA (and probably Phc) positive
20 le beta-oxidation operon deletion mutants
of R. eutropha could grow using palm oil or crude palm kern
21 We also demonstrate that
some R. eutropha produce an extracellular factor that complem
22 Although our data strongly suggest
that R. eutropha has a functional Phc-like system and support
23 The results suggest
that R. eutropha has evolved a regulatory mechanism that can
24 n blot hybridization analysis suggested
that R. eutropha harbours other Phc components, such as PhcB
25 I synthases revealed similar residues in
the R. eutropha synthase.
26 Sequencing and analysis of
the R. eutropha genome revealed putative isologs for each of
27 Transfer of
the R. eutropha phaR, phaP, and PHA biosynthesis (phaCAB) ge
28 These primers and PCR methods on
the R. eutropha genome were used to identify two new candida
29 Similarly,
the R. eutropha phcA mutant was unaffected in siderophore pr
30 These studies reveal that
the R. eutropha synthase possesses an essential catalytic dy