ライフサイエンスコーパス: R. leguminosarum

1 R. leguminosarum lipid A is esterified with a peculiar l

2 R. leguminosarum lipid A lacks phosphate groups, but it

3 R. leguminosarum lipid A lacks the usual 1- and 4'-phosp

4 R. leguminosarum lipid A often contains an aminogluconic

5 R. leguminosarum LpxC therefore provides a useful contro

6 ing 1800 lysates of individual colonies of a R. leguminosarum 3841 genomic DNA library in the host st

7 We have identified an R. leguminosarum autoinducer that, together with RhiR, i

8 ly 4000 lysates of individual colonies of an R. leguminosarum 3841 genomic DNA library in the host st

9 lipid A precursor common to both E. coli and R. leguminosarum.

10 horylated at positions 1 and 4', R. etli and R. leguminosarum lipid A consists of a mixture of struct

11 The homologous interaction between R. leguminosarum bv viciae and its host, pea, was examin

12 crease in transcriptional activation in both R. leguminosarum and S. meliloti.

13 ant roots were even found to be colonized by R. leguminosarum bv viciae expressing S. meliloti nod ge

14 acidic exopolysaccharides (EPSs) produced by R. leguminosarum in a calcium-dependent manner, sustaini

15 ich the chromosomal lpxC gene is replaced by R. leguminosarum lpxC is resistant to CHIR-090 up to 100

16 ive amounts of AHLs synthesized over time by R. leguminosarum cells with and without the symbiosis pl

17 7 promoter results in the production of each R. leguminosarum glycosyltransferase in E. coli membrane

18 sual structural features shared with R. etli/R. leguminosarum lipid A may be essential for symbiosis.

19 t roots following inoculation with an Exo(-) R. leguminosarum bv viciae strain that produced S. melil

20 s in overproduction in vitro of the expected R. leguminosarum acyltransferase, which is C28-AcpXL-dep

21 Aap from R. leguminosarum expressed in E. coli also promoted effl

22 now describe a membrane-bound deacylase from R. leguminosarum that removes a single ester-linked beta

23 brane enzyme and a cytosolic acyl donor from R. leguminosarum, that transfers 27-hydroxyoctacosanoic

24 ae or is more widespread, cell extracts from R. leguminosarum, Rhizobium sp. strain NGR234, Rhizobium

25 n both membranes and a cytosolic factor from R. leguminosarum.

26 esis, a 12.5 kDa protein was identified from R. leguminosarum as a putative homolog of IHF subunit be

27 in the E. coli system when total lipids from R. leguminosarum 3841 or S. meliloti 1021 were added.

28 zorhizobium caulinodans may be secreted from R. leguminosarum bv. viciae in a prsD-dependent manner.

29 induction (ini) in response to signals from R. leguminosarum bv viciae.

30 In R. leguminosarum lipopolysaccharide, the inner core is m

31 icate that the synthesis of multiple AHLs in R. leguminosarum is regulated by complex mechanisms that

32 The mutation was created in R. leguminosarum bv. phaseoli strain 8002, which forms s

33 lyze any of the unique reactions detected in R. leguminosarum extracts.

34 proposed to represent a key early enzyme in R. leguminosarum core assembly.

35 ages of heptose in E. coli and of mannose in R. leguminosarum to Kdo are both alpha1-5.

36 and the absence of laurate and myristate in R. leguminosarum.

37 sessment of the role of the 1-phosphatase in R. leguminosarum symbiosis with plants.

38 coli generates the same products as seen in R. leguminosarum membranes.

39 membrane-associated glycosyl transferase in R. leguminosarum extracts that incorporates mannose into

40 osyl-(beta1-->4)-glucuronosyl transferase in R. leguminosarum.

41 f three novel GalA transferases from a 22-kb R. leguminosarum genomic DNA insert-containing cosmid (p

42 a hybrid cosmid (pMJK-1) containing a 25-kb R. leguminosarum 3841 DNA insert that directs the overex

43 he KM (4.8 microM) and the kcat (1.7 s-1) of R. leguminosarum LpxC with UDP-3-O-[(R)-3-hydroxymyristo

44 , or the nodulation-defective mutant 24AR of R. leguminosarum.

45 atty acid (VLCFA) is found in the lipid A of R. leguminosarum as well as in the lipid A of the medica

46 sters modulate the motility swimming bias of R. leguminosarum cells and that the che1 cluster is the

47 spite these differences, the biosynthesis of R. leguminosarum lipid A is initiated by the same seven

48 ort the purification and characterization of R. leguminosarum LpxE.

49 nvironmental cue(s) triggering chemotaxis of R. leguminosarum bv. viciae cells towards the roots of p

50 o-heptose (heptose), while the inner core of R. leguminosarum contains 2-keto-3-deoxy-D-manno-octulos

51 ram-negative bacteria, and the inner core of R. leguminosarum contains mannose and galactose in place

52 though PtdIns is not detected in cultures of R. leguminosarum/etli (CE3), PtdIns may be synthesized d

53 ve responses and the symbiotic efficiency of R. leguminosarum.

54 The GDP-mannose-dependent enzyme of R. leguminosarum may represent a functional equivalent o

55 ve previously been identified in extracts of R. leguminosarum and Rhizobium etli but not Sinorhizobiu

56 An especially striking feature of R. leguminosarum lipid A is that it lacks both the 1- an

57 The genome of R. leguminosarum bv. viciae 3841, a pea-nodulating endos

58 hat lipid A isolated by pH 4.5 hydrolysis of R. leguminosarum cells is more heterogeneous than previo

59 ntribution of chemotaxis to the lifestyle of R. leguminosarum, we have characterized the function of

60 60) phosphate-GalA as a minor novel lipid of R. leguminosarum 3841 and S. meliloti.

61 located exclusively in the outer membrane of R. leguminosarum as judged by sucrose gradient analysis.

62 taining precursor is present in membranes of R. leguminosarum and R. etli but not in S. meliloti or E

63 We now demonstrate that membranes of R. leguminosarum and R. etli can convert B to D-1 in a r

64 P-mannose and/or UDP-galactose, membranes of R. leguminosarum first transferred mannose and then gala

65 ation of CLOS also enabled a NodC- mutant of R. leguminosarum bv. trifolii to progress further in the

66 S. meliloti lpsB complements a mutant of R. leguminosarum defective in lpcC, but the converse doe

67 In this paper, a mutant of R. leguminosarum was created by placing a kanamycin resi

68 The double sitA mntH mutant of R. leguminosarum was unable to fix nitrogen (Fix(-) ) wi

69 the inducible acyl carrier protein (NodF) of R. leguminosarum.

70 ch with a partially sequenced gene (orf*) of R. leguminosarum.

71 which contains at least 20 kilobase pairs of R. leguminosarum DNA.

72 Rhizobium NGR234 and R. meliloti, and Psi of R. leguminosarum bv. phaseoli.

73 was introduced into the IHF binding site of R. leguminosarum dtA that reduced the affinity of the pr

74 it inhibits the growth of several strains of R. leguminosarum and was previously known as 'small bact

75 We hypothesize that R. leguminosarum bv. viciae 3841 contains an alternate m

76 ess waaC-waaF deletion mutant expressing the R. leguminosarum lpcC gene likewise generates a hybrid L

77 mediated transcriptional activation from the R. leguminosarum dctA promoter both in vivo and in vitro

78 mediated transcriptional activation from the R. leguminosarum dctA promoter.

79 The inner portion of the R. leguminosarum core contains mannose, galactose, and t

80 Purified CinS bound to the R. leguminosarum transcriptional regulator PraR, which r

81 In contrast to R. leguminosarum dctA, the Sinorhizobium meliloti dctA p

82 ed infection thread formation in response to R. leguminosarum bv viciae, but only when the bacteria e

83 solic acyl donor was purified from wild-type R. leguminosarum using the acylation of (Kdo)2-[4'-32P]-

84 ndary acyl chains attached to E. coli versus R. leguminosarum lipid A, specifically the presence of 2

85 and detoxification in plants inoculated with R. leguminosarum has particular relevance to PGPB enhanc

86 ance to Zn was associated predominantly with R. leguminosarum and was likely due to the coordination