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1                                              R. sphaeroides CheAs exhibit two interesting differences
2                                              R. sphaeroides CheYs did not affect E. coli lacking CheY
3                                              R. sphaeroides DMSOR expressed in a mobA(-) Escherichia
4                                              R. sphaeroides has a single, plain filament whose confor
5                                              R. sphaeroides moves in a series of runs and stops produ
6                                              R. sphaeroides mutants which lacked either cyt c2 or cyt
7                                              R. sphaeroides possesses a phosphorelay cascade compatib
8                                              R. sphaeroides sigma37 controls genes that function duri
9                                         In a R. sphaeroides mutant lacking RsAgo, expression of plasm
10                                Additionally, R. sphaeroides CbbR was shown to bind to the region betw
11 re as E. coli CheA and can phosphorylate all R. sphaeroides chemotaxis response regulators.
12                                           An R. sphaeroides transposon mutant in this gene, bchP, pos
13 us reg locus was capable of complementing an R. sphaeroides regA-deficient mutant to phototrophic gro
14                              For example, an R. sphaeroides deltaRpoH mutant is not generally defecti
15 and RoxR enabled photosynthetic growth of an R. sphaeroides PrrA mutant.
16 nction during environmental stress, since an R. sphaeroides deltaRpoH mutant is approximately 30-fold
17    In heterologous complementation analysis, R. sphaeroides PrrA rescued the growth defect of a P. ae
18 d small subunit genes from R. capsulatus and R. sphaeroides, also supported the unrelatedness of thes
19 avior as wild-type oxidase with horse Cc and R. sphaeroides Cc(2), showing that these residues are no
20 onstructed and expressed in both E. coli and R. sphaeroides.
21      However, their reactions with horse and R. sphaeroides Cc are different, as expected from the di
22                           Both lipid IVA and R. sphaeroides lipid A inhibited the effects of LPS and
23  be present in the nondenitrifying bacterium R. sphaeroides 2.4.1.
24 larity and evolutionary relationship between R. sphaeroides and mitochondria, our data suggest that t
25 B (prrB) gene, previously isolated from both R. sphaeroides and Rhodobacter capsulatus and shown to r
26  inactive, trapped P(+)Q(B)(-) state in both R. sphaeroides and B. viridis RCs that does not recombin
27 was cloned, sequenced, and expressed in both R. sphaeroides and Escherichia coli.
28 ute for the assimilation of this molecule by R. sphaeroides.
29                           AdoCbl produced by R. sphaeroides was identified and quantified by high-per
30 roidenone, are differentially synthesized by R. sphaeroides, depending on the growth conditions.
31  gene expression are the major means used by R. sphaeroides in adaptation to diverse conditions.
32  of the cbbXYZ gene products, we constructed R. sphaeroides strains in which the genes were inactivat
33 om an overexpressing variant of denitrifying R. sphaeroides 2.4.3 was investigated by proton, nitroge
34 er with increased activity when using either R. sphaeroides RNA polymerase or Escherichia coli Esigma
35                                  To evaluate R. sphaeroides transcriptome flexibility, expression pro
36 .3 x 10(4) s-1 and k-1 = 1.7 x 10(4) s-1 for R. sphaeroides cytochrome oxidase in the resting state.
37  species but, unlike these, is essential for R. sphaeroides chemotaxis to all compounds tested.
38 ructure was modeled using the structures for R. sphaeroides dimethyl sulfoxide reductase, Rhodobacter
39                                    AcuI from R. sphaeroides catalyzes the NADPH-dependent acrylyl-CoA
40 second operon (groESL2) was also cloned from R. sphaeroides, using a groEL1 gene fragment as a probe;
41              Cytochrome c oxidase (COX) from R. sphaeroides contains one Ca(2+) ion per enzyme that i
42 this conserved glutamate in the oxidase from R. sphaeroides (E101(II)I,A,C,Q,D,N,H) and residues in t
43 3 in subunit II of cytochrome c oxidase from R. sphaeroides) is not involved in the dominant tunnelin
44 ted mutagenesis using the cbb3 oxidases from R. sphaeroides and Vibrio cholerae.
45 ion of a 24-kDa IF3-like protein (PifC) from R. sphaeroides, we used nested PCR to clone and characte
46 its/nmol molybdenum for enzyme purified from R. sphaeroides.
47 e by crotonyl-CoA carboxylase/reductase from R. sphaeroides was attributed to the fact that the enzym
48                                     However, R. sphaeroides cyt cy, unlike that of R. capsulatus, is
49                     To test this hypothesis, R. sphaeroides mutants expressing His6-tagged bc1 comple
50 d in the vicinity of the recently identified R. sphaeroides 2.4.1 homolog of the anaerobic regulatory
51                                           In R. sphaeroides, synthesis of a novel homoserine lactone
52 lographic structures (His-217 and Asp-252 in R. sphaeroides).
53   The conserved tyrosine residue (Tyr-288 in R. sphaeroides, Tyr-244 in the bovine enzyme) that is ad
54 hese results suggest a model where Cyt c' in R. sphaeroides 2.4.3 may shuttle NO to the membrane, whe
55                      Inactivation of cerI in R. sphaeroides results in mucoid colony formation on aga
56 own that the expression of chemoreceptors in R. sphaeroides varies with growth conditions.
57 f proteins required for normal chemotaxis in R. sphaeroides is all the proteins encoded by cheOp2 and
58 A homologues are essential for chemotaxis in R. sphaeroides under laboratory conditions.
59 , and CheA(4) is essential for chemotaxis in R. sphaeroides.
60  catalytic mechanism of the bc(1) complex in R. sphaeroides.
61 S, which are utilized for denitrification in R. sphaeroides 2.4.3.
62 nt transcription termination is essential in R. sphaeroides.
63 rried on multicopy plasmids was expressed in R. sphaeroides under the direction of its own promoter,
64              Because ppsR gene expression in R. sphaeroides 2.4.1 appears to be largely independent o
65 n change the specific activity of GSH-FDH in R. sphaeroides extracts.
66 s of the primary pathway for CO2 fixation in R. sphaeroides, regB was also found to be required for t
67 ep in enabling one to model electron flow in R. sphaeroides 2.4.1.
68 ple chemosensory protein homologues found in R. sphaeroides are not redundant.
69  the molybdenum as compared to that found in R. sphaeroides DMSO reductase demonstrated the presence
70 rificans promoters were shown to function in R. sphaeroides, resulting in high levels of cbbL cbbS an
71 ded by two differentially regulated genes in R. sphaeroides 2.4.1: hemA and hemT.
72 acement of the wild-type chemotaxis genes in R. sphaeroides with their corresponding fluorescent prot
73 li Tsr, MCP-like proteins were identified in R. sphaeroides WS8N.
74 onfirming its role and function as an IF3 in R. sphaeroides.
75 ters were consistent with prior knowledge in R. sphaeroides and/or other bacteria.
76 odel in which the multiple different MCPs in R. sphaeroides are contained within a polar chemorecepto
77 the regulation of tetrapyrrole metabolism in R. sphaeroides.
78                       Each of the mutants in R. sphaeroides, with an exception at only one position,
79 nerally correlated with the data obtained in R. sphaeroides and support the computer predictions.
80 een the first two genes of the cco operon in R. sphaeroides 2.4.1, which encodes a cytochrome c termi
81 203 identified a second chemotaxis operon in R. sphaeroides that contains homologues of cheY, cheA an
82 r and multiple sensory signaling pathways in R. sphaeroides generate the same swimming response as se
83 bacteriochlorophyll biosynthetic pathways in R. sphaeroides in response to the availability of molecu
84 he existence of two chemosensory pathways in R. sphaeroides, a feature that so far is unique in bacte
85        Overexpression of full-length PcrZ in R. sphaeroides affects expression of a small subset of g
86 II) produced a chemotaxis minus phenotype in R. sphaeroides, suggesting that cheA(II) forms part of a
87  the assembly of a functional photosystem in R. sphaeroides, a model organism for the study of struct
88 biosynthesis of all tetrapyrroles present in R. sphaeroides 2.4.1.
89 olve the interacting redox chains present in R. sphaeroides under diverse growth conditions, and many
90 r either of the cyt c oxidases is present in R. sphaeroides.
91            Additionally, the FnrL protein in R. sphaeroides is required for anaerobic growth in the d
92  may act as a signal-transduction protein in R. sphaeroides, it may have an unusual role in controlli
93  multiple copies of chemosensory proteins in R. sphaeroides.
94 pression of genes encoding DMSO reductase in R. sphaeroides and identify the DorS-DorR proteins as a
95 m with a central role in redox regulation in R. sphaeroides.
96 blished for hemA transcription regulation in R. sphaeroides.
97 stribution of members of the NnrR regulon in R. sphaeroides revealed patterns of coselection of struc
98 f these findings for cobamide remodelling in R. sphaeroides and in other CbiZ-containing microorganis
99                     The lethality of rho' in R. sphaeroides together with our inability to obtain a n
100 ting that CcmG has another important role in R. sphaeroides.
101  promoters that respond to (1)O(2) stress in R. sphaeroides.
102 nd biochemical evidence we conclude that, in R. sphaeroides, the activity of the cobyric acid-produci
103                                Therefore, in R. sphaeroides, the global PrrBA system regulates photos
104  Rhodobacter sphaeroides and introduced into R. sphaeroides by using a broad-host-range vector.
105 stance to nitrite when it was mobilized into R. sphaeroides strain 2.4.1 containing nirK.
106          The polymorphic ability of isolated R. sphaeroides filaments was tested in vitro by varying
107 moters were experimentally validated to nine R. sphaeroides sigma(E)-dependent promoters that control
108                                 Nonetheless, R. sphaeroides cyt cy can act at least in R. capsulatus
109 ional strains (ATCC 17019 and ATCC 17025) of R. sphaeroides have been sequenced.
110 trol mechanism involved in the adaptation of R. sphaeroides to changes in light intensity and oxygen
111   Finally, we found that the same aspects of R. sphaeroides ChrR needed for a response to (1)O(2) are
112 t parameter selection produces assemblies of R. sphaeroides comparable to or exceeding the quality of
113 e tethered photosynthetically grown cells of R. sphaeroides by their flagella and measured the respon
114 an ancient partnership between CI and CII of R. sphaeroides 2.4.1.
115      Aside from the size variation of CII of R. sphaeroides, variation in sequence lengths of the CII
116 m) are localized in separate compartments of R. sphaeroides.
117 rations of spermidine in growing cultures of R. sphaeroides gave rise to a twofold increase in the ex
118 oli delta cheW mutants, in-frame deletion of R. sphaeroides cheW did not affect either swimming behav
119                                Expression of R. sphaeroides cheW in an E. coli delta cheW chemotaxis
120                                Expression of R. sphaeroides cheW in Escherichia coli showed concentra
121 opionyl-CoA was detected in cell extracts of R. sphaeroides grown with 3-hydroxypropionate, and both
122 er region were detected in crude extracts of R. sphaeroides.
123  transcription of the cbb(I) operon genes of R. sphaeroides.
124                                The genome of R. sphaeroides 2.4.1 has been completely sequenced and f
125 ne for Surf1p was deleted from the genome of R. sphaeroides.
126  constitutive level throughout the growth of R. sphaeroides 2.4.3.
127 ion is critical for photosynthetic growth of R. sphaeroides.
128 nome restriction maps (EcoRI and HindIII) of R. sphaeroides strain 2.4.1 were constructed using shotg
129 at the aspartic residue D442 (a homologue of R. sphaeroides D485) may be the second Na(+) binding sit
130 phoresis, isolated recombinant subunit IV of R. sphaeroides cytochrome b-c1 complex.
131 pothesis that the photosynthetic membrane of R. sphaeroides 2.4.1 contains a significant number of he
132 diffusion of quinones in native membranes of R. sphaeroides are discussed.
133 ctly, the levels of TspO in the membranes of R. sphaeroides.
134 1914 or yhdH into a DeltaacuI::kan mutant of R. sphaeroides on a plasmid complemented 3-hydroxypropio
135 aging of membranes prepared from a mutant of R. sphaeroides, DPF2G, that synthesizes only the LH2 com
136                                   Mutants of R. sphaeroides deficient in the biosynthesis of the beta
137 tudy, however, we found that PrrA mutants of R. sphaeroides were capable of chemoautotrophic growth,
138 gths of sequence 5' to the cbb(II) operon of R. sphaeroides CAC.
139               In contrast, overexpression of R. sphaeroides cheW in wild-type R. sphaeroides inhibite
140 enzymes of coproporphyrinogen III oxidase of R. sphaeroides, provided in trans to the wild type strai
141 e respiratory electron transport pathways of R. sphaeroides are more complex than those of R. capsula
142  light of our knowledge of the physiology of R. sphaeroides under aerobic and photosynthetic growth c
143      Three mutants at the Asp407 position of R. sphaeroides cytochrome oxidase, Asp407Ala, Asp407Asn,
144                            The properties of R. sphaeroides cells containing translational fusions be
145  of photosystem biosynthesis, regB (prrB) of R. sphaeroides is intimately involved in the positive re
146  Rhodobacter capsulatus, a close relative of R. sphaeroides.
147            In characterizing the response of R. sphaeroides to heat, we found that its growth tempera
148  we present the annotated genome sequence of R. sphaeroides WS8N.
149 hromosome II (CII)-specific DNA sequences of R. sphaeroides have rapidly evolved, while CI-specific D
150                                  A series of R. sphaeroides mutants with replacements of the E54, Q61
151 ation of the copper binding stoichiometry of R. sphaeroides Cox11 led to the finding that an apparent
152 ural genes of the cbb regulon in a strain of R. sphaeroides capable of aerobic CO2-dependent growth i
153 iosynthetic pathway) resulted in a strain of R. sphaeroides that would not grow on acetate in the abs
154                  2.4.3 is the only strain of R. sphaeroides with norEF, even though all four of the s
155 e occurrence of norEF in the 2.4.3 strain of R. sphaeroides, which can reduce nitrate to nitrous oxid
156  used to examine approximately 25 strains of R. sphaeroides in an effort to assess the occurrence of
157 ir high sequence divergence among strains of R. sphaeroides suggest the involvement of CII in the evo
158 omosomal sequences from the three strains of R. sphaeroides were aligned, using Mauve, to examine the
159 ary relationships among different strains of R. sphaeroides.
160 s accumulated by resting cell suspensions of R. sphaeroides, we demonstrated that TspO negatively reg
161                               The termini of R. sphaeroides flagellin are predicted to have a lower p
162 s study illustrates the potential utility of R. sphaeroides and the coxII promoter for heterologous e
163                       A truncated version of R. sphaeroides Rho (Rho') is toxic to a bacterium relate
164 verexpression of PgsARs in either E. coli or R. sphaeroides did not have dramatic effects on the phos
165 n all high-resolution structures of oxidized R. sphaeroides cytochrome c oxidase, a water molecule is
166 nd MreB in aerobic and in photoheterotrophic R. sphaeroides cells using fluorescence microscopy.
167  in vitro transcription assays with purified R. sphaeroides core RNA polymerase and sigma(E), we show
168   In common with other ALASs the recombinant R. sphaeroides HemA requires pyridoxal 5'-phosphate (PLP
169                                        Since R. sphaeroides harbors both a cbb3-Cox and an aa3-type c
170 G are sufficient for TTQ biosynthesis, since R. sphaeroides cannot synthesize TTQ.
171 bility of each sigma factor to recognize six R. sphaeroides promoters (cycA P1, groESL(1), rpoD P(HS)
172                                   While some R. sphaeroides proteins restore tumbling to smooth-swimm
173  fraction of another representative species, R. sphaeroides, but it was completely unaffected by anti
174 the three classes of tRFs for eight species: R. sphaeroides, S. pombe, D. melanogaster, C. elegans, X
175            In contrast to wild-type strains, R. sphaeroides and R. capsulatus fnrL mutants do not syn
176                                Free-swimming R. sphaeroides was examined by both differential interfe
177        Using this assay, we demonstrate that R. sphaeroides can utilize 3-hydroxypropionate as the so
178                 Our results demonstrate that R. sphaeroides exhibits persistence over the course of a
179 o acid sequence comparisons demonstrate that R. sphaeroides RpoH(II) belongs to a phylogenetically di
180              Despite this, and the fact that R. sphaeroides is widely used for the study of structure
181    DNase I footprint analyses indicated that R. sphaeroides CbbR binds to the cbb(I) promoter between
182                          Here we report that R. sphaeroides strain 2.4.1 synthesizes AdoCbl de novo a
183 ree-dimensional swimming pattern showed that R. sphaeroides changed speed while swimming, sometimes d
184 y NMR spectroscopy cumulatively suggest that R. sphaeroides GSH-FDH can play a critical role in forma
185                                          The R. sphaeroides groESL1 operon contains a putative hairpi
186         The range of transitions made by the R. sphaeroides filament differs from that reported for S
187      When expressed in Escherichia coli, the R. sphaeroides rho gene relieves Rho-dependent polarity
188 Most of the DNA sequence duplications in the R. sphaeroides genome occurred early in species history,
189 over the history of gene duplications in the R. sphaeroides genome, 44 gene duplications were sampled
190 vels of cbbL cbbS and cbbM expression in the R. sphaeroides host.
191 me a to oxyferryl heme a3 is the same in the R. sphaeroides K362M CcO mutant as in wild-type CcO, ind
192 y inactive enzyme in the set examined in the R. sphaeroides oxidase are in R52, a residue that, along
193 ons N338T and N338A were introduced into the R. sphaeroides protein by site-directed mutagenesis to d
194 ications occurred prior to the origin of the R. sphaeroides 2.4.1 lineage.
195                          The sequence of the R. sphaeroides fliC gene, which encodes the flagellin pr
196 sion levels of one-fifth to one-third of the R. sphaeroides ORFs were significantly different in cell
197 trating that the alpha and beta forms of the R. sphaeroides oxidase exist at room temperature; theref
198                      The N139D mutant of the R. sphaeroides oxidase was further characterized by exam
199 eaction was studied using two mutants of the R. sphaeroides oxidase, K362M and D132N, that block, res
200 produced by integral membrane enzymes of the R. sphaeroides photosynthetic apparatus.
201                             Knowledge of the R. sphaeroides response to (1)O(2) and its regulator sig
202                 To relate the context of the R. sphaeroides trp genes to those of other bacteria, the
203 under the direction of its own promoter, the R. sphaeroides rrnB promoter, and the E. coli lac promot
204           The genetic region surrounding the R. sphaeroides rho gene has been determined and found to
205       We present data demonstrating that the R. sphaeroides genome possesses an extensive amount of e
206 on by using translational lac fusions to the R. sphaeroides cbb(I) and cbb(II) promoters.
207 ata from the literature, thus validating the R. sphaeroides genechip as a powerful and reliable tool
208                                  Whereas the R. sphaeroides signal sequence prevents formation of act
209 1(DE3) cells in its mature form and with the R. sphaeroides or E. coli N-terminal signal sequence.
210 , we found a fourth cheY and expressed these R. sphaeroides proteins in E. coli.
211 rimental validation of predictions from this R. sphaeroides TRN model showed that high precision and
212 ide (DMSO) or trimethylamine N-oxide (TMAO), R. sphaeroides 2.4.1T utilizes DMSO or TMAO as the termi
213 , Paracoccus denitrificans, and is lethal to R. sphaeroides.
214 ho (Rho') is toxic to a bacterium related to R. sphaeroides, Paracoccus denitrificans, and is lethal
215 logue, found in a species closely related to R. sphaeroides, than to its duplicate, counterpart allel
216 th increased information content relative to R. sphaeroides TRN models built via other approaches.
217 e chromatin immunoprecipitation technique to R. sphaeroides.
218 pression of R. sphaeroides cheW in wild-type R. sphaeroides inhibited motility completely, the equiva
219 cyt c oxidase (aa3-Cox), we examined whether R. sphaeroides cyt cy can act as an electron carrier to
220 expected for a "scotophobic" response, while R. sphaeroides, which stops rather than reverses, accumu
221                         Possible reasons why R. sphaeroides maintains two distinct pathways for Cbi s
222             While RpoH(I) reconstituted with R. sphaeroides core RNA polymerase transcribed all six p
223 ain sigma(E)-dependent promoters both within R. sphaeroides and across the bacterial phylogeny.

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