ライフサイエンスコーパス: R. typhi

1 LipoP, SignalP, and Phobius, a total of 191 R. typhi proteins were predicted to contain signal pepti

2 f PI(3,4,5)P3 and PI(3)P, negatively affects R. typhi infection.

3 ot analyses of ELB, with typing sera against R. typhi indicate that ELB surface antigens are more clo

4 d LDH release demonstrated that R. akari and R. typhi caused negligible cytotoxicity in mouse periton

5 more, macrophage infection with R. akari and R. typhi resulted in differential synthesis and expressi

6 cytokines was observed between R. akari- and R. typhi-infected macrophages, which may correlate with

7 Both the R. prowazekii Breinl and R. typhi Wilmington metK genes complemented at a level c

8 . typhi, 15 are found only in R. conorii and R. typhi, and 24 are unique to R. typhi.

9 occurred after the typhus (R. prowazekii and R. typhi) and spotted fever (R. conorii) groups diverged

10 enes: 23 are found only in R. prowazekii and R. typhi, 15 are found only in R. conorii and R. typhi,

11 ettsiae, including Rickettsia prowazekii and R. typhi, produce visible plaques on primary chick embry

12 The cloned lepB genes from R. rickettsii and R. typhi have been demonstrated to possess signal peptid

13 the cloned lepB genes from R. rickettsii and R. typhi shows open reading frames of 801 and 795 nucleo

14 Accordingly, we assessed R. typhi-mediated flea IMD pathway activation in vivo us

15 s are more closely related to the flea-borne R. typhi than to the mite-borne R. akari.

16 Complementation assay by R. typhi groESL in a temperature sensitive Escherichia c

17 ar mechanism underlying erythrocyte lysis by R. typhi has not been defined.

18 RARP-1 was secreted by R. typhi into the host cytoplasm during in vitro infecti

19 The availability of the cloned R. typhi tlyC will permit further characterization and d

20 ctive was to characterize such Sec-dependent R. typhi proteins in the context of a mammalian host cel

21 Secreted early during R. typhi infection, RalF localizes to the host plasma me

22 of growth suggests its potential role during R. typhi infection of host cells.

23 sponding to the requirement of PI(4,5)P2 for R. typhi entry.

24 -specific restimulation of spleen cells from R. typhi(GFPuv)-infected BALB/c mice elicits gamma inter

25 of the gene (tlyC) encoding a hemolysin from R. typhi.

26 netic complementation, recombinant lspA from R. typhi significantly restores the growth of temperatur

27 RT0776, and (ii) native OmpBs purified from R. typhi and R. prowazekii strains Breinl, RP22, and Mad

28 We identified 877 genes in R. typhi encoding 3 rRNAs, 33 tRNAs, 3 noncoding RNAs, a

29 ich is close to the replication terminus, in R. typhi, compared to R. prowazekii and R. conorii.

30 Knockdown of Relish and Imd increased R. typhi infection levels, implicating the IMD pathway a

31 In addition, we found a 124-kb R. typhi-specific inversion, starting 19 kb from the ori

32 DNA sequence analysis of R. typhi tlyC revealed an open reading frame of 912 bp,

33 es of Rickettsia prowazekii RP027-028 and of R. typhi RT0101 and to monomethyltransferases of R. prow

34 yltransferases of R. prowazekii RP789 and of R. typhi RT0776, and (ii) native OmpBs purified from R.

35 Importantly, expression of R. typhi tolC in the E. coli tolC mutant restored the se

36 bed at all stages of intracellular growth of R. typhi in Vero cells.

37 Overexpression of R. typhi lspA in Escherichia coli confers increased glob

38 al transcript abundance at various phases of R. typhi intracellular growth.

39 ntified it as a secreted effector protein of R. typhi.

40 g the IMD pathway as a critical regulator of R. typhi burden in C. felis These data suggest that targ

41 e we present the complete genome sequence of R. typhi (1,111,496 bp) and compare it to the two publis

42 The deduced amino acid sequence of R. typhi GroES and GroEL shows a high degree of identity

43 the IMD pathway could minimize the spread of R. typhi, and potentially other human pathogens, vectore

44 t of the putative type 1 secretion system of R. typhi is involved in the secretion process of RARP-1.

45 ere for the first time the transformation of R. typhi with the pRAM18dRGA plasmid that originally der

46 of BA6163) transformed with R. typhi tlyC or R. typhi GFPuv-tlyC constructs.

47 a genome sequence, little is known regarding R. typhi biology in flea vectors that, importantly, do n

48 Typhus group rickettsiae (R. typhi and R. prowazekii) adhere to and lyse human and

49 se to R. typhi Initially, we determined that R. typhi infects Drosophila cells and increases antimicr

50 To associate the R. typhi tlyC gene product with hemolytic activity, we p

51 l analysis indicated that at least 54 of the R. typhi extracytoplasmic proteins undergo active gene e

52 n functionally characterized; therefore, the R. typhi Sec apparatus represents a mechanism for the se

53 ection, with GTP-bound Arf6 localized to the R. typhi entry foci.

54 Thus, R. typhi(GFPuv) bacteria are a novel, potent tool to stu

55 importantly, do not suffer lethality due to R. typhi infection.

56 ion in the R. prowazekii genome, relative to R. typhi and R. conorii, which appears to have occurred

57 ocephalides felis) innate immune response to R. typhi Initially, we determined that R. typhi infects

58 . conorii and R. typhi, and 24 are unique to R. typhi.

59 Transformed R. typhi (R. typhi(GFPuv)) bacteria are viable, replicat

60 tection of GFPUV expression, and transformed R. typhi were isolated in a fluorescence-activated cell

61 to those for animals infected with wild-type R. typhi and develop comparable pathology and bacterial

62 with kinetics similar to those of wild-type R. typhi in cell culture, and stably maintain the plasmi

63 Transformed R. typhi (R. typhi(GFPuv)) bacteria are viable, replicate with kin

64 Native OmpB from virulent R. typhi contains mono- and trimethyllysines at location

65 single cluster in OmpB from mildly virulent R. typhi.

66 CB17 SCID mice infected with R. typhi(GFPuv) succumb to the infection with kinetics s

67 a HpmA(-) mutant of BA6163) transformed with R. typhi tlyC or R. typhi GFPuv-tlyC constructs.