ライフサイエンスコーパス: RACE

1 RACE (Rapid Amplification of cDNA Ends) identified a 1.5

2 RACE (rapid amplification of cDNA ends) PCR is useful fo

3 RACE analysis extended the sequence and identified this

4 RACE and RT-PCR identified a splice variant of FKBP8 lac

5 RACE describes all alveoli that visibly change volume wi

6 RACE experiments then mapped the 3' terminus of the upst

7 RACE is 55-330 times faster and 2-5 times more accurate

8 RACE is easy to use, as it requires adjustment of only t

9 RACE mapping revealed that lpiA/acvB were co-transcribed

10 RACE processed these terabyte-sized datasets on a single

11 RACE was used to identify one major and two minor transc

12 3' RACE provided evidence for variability in the 3' UTR due

13 Real-time PCR and 5' RACE and 3' RACE experiments revealed that this locus encodes two tr

14 for the QQS mRNA was identified by 5' and 3' RACE experiments.

15 were obtained by assembling their 5' and 3' RACE polymerase chain reaction products.

16 cloned from nodule mRNA by the RT-PCR and 3' RACE procedures.

17 hain reaction (RT-PCR) followed by 5' and 3' RACE showed that Sh-NOS is a protein of 1,517 amino acid

18 lable immunoglobulin sequences and 5' and 3' RACE to clone and sequence heavy and light chain immunog

19 some 2 were designed, and, through 5' and 3' RACE, clones from 506 genes were sequenced and cDNA sequ

20 nce, reverse transcription-PCR and 5' and 3' RACE.

21 bout 6 kb through conventional RT-PCR and 3' RACE.

22 gene structures were delineated by 5' and 3' RACE.

23 and polyadenylation sites were defined by 3' RACE.

24 In 3' RACE, the poly(A) tail functions as a non-specific tag a

25 o nt 7299 or 7307, respectively, by using 3' RACE.

26 Examination of mGL50 cDNA transcripts by 3'RACE revealed an alternatively spliced form, mGL50-B, th

27 Using 3'RACE, we confirmed expression of two distinct forms of t

28 In addition, using 3'RACE, we identified three previously unknown mGlu1 recep

29 5'-and 3'-RACE experiments have subsequently identified a 900-bp c

30 5'- and 3'-RACE experiments identified a total of 11 R. reniformis

31 T47D breast carcinoma cells by RT-PCR and 3'-RACE PCR and identified a novel extended form of QSOX1 t

32 e use of these conditions yielded 5'- and 3'-RACE products that were approximately 80% GC over 213 an

33 108-158 nt) through genomic analysis and 3'-RACE technique, which was confirmed by RNA blot analysis

34 As an initial step, we performed 5'- and 3'-RACE with SULT1C1 cDNA.

35 ed and verified using RPE/choroid 5'- and 3'-RACE.

36 uence of MIH, in conjunction with 5'- and 3'-RACE.

37 RNA-sequencing data, followed by 5'- and 3'-RACE.

38 ctive polyadenylation sites identified by 3'-RACE are conserved in human, mouse, and chicken SCN8A.

39 from the one for UT-A2 and identified by 3'-RACE new transcripts of UT-A1, UT-A2, and UT-A3, charact

40 5'-methylguanosine-capped RNA followed by 3'-RACE sequencing.

41 amplification of complementary DNA ends (3'-RACE) polymerase chain reaction, we identified a chimeri

42 We used 5'-or 3'-RACE, GenomeWalking, reverse transcriptase polymerase ch

43 Subsequent 3'-RACE, 5'-RACE and high fidelity PCR were then used to pr

44 d RNA-Seq procedures, as well as a 1200 bp 5 RACE product coupled with PACBio sequencing that can ide

45 Using 5"-RACE we have identified two novel transcripts of utrophi

46 5' RACE analysis revealed the presence of a previously unde

47 5' RACE analysis revealed the presence of the transcription

48 5' RACE analysis was performed to determine the functional

49 5' RACE analysis, promoter-reporter assays, and semiquantit

50 5' RACE carried out with CWR22Rv1 and HepG2 cells detected

51 5' RACE indicates a transcription start site for HYDIN2 out

52 5' RACE studies localized the initiation sites to a <100 bp

53 l clones in mediating tumor regression, a 5' RACE technique was used to determine the distribution of

54 Real-time PCR and 5' RACE and 3' RACE experiments revealed that this locus en

55 validation using RNA cleavage assays, and 5' RACE identified the prooncogenic basic helix-loop-helix

56 obtained by further rounds of RT-PCR and 5' RACE procedures.

57 that gene was then cloned by both 3' and 5' RACE.

58 tained by exon amplification, RT-PCR, and 5' RACE.

59 ' end of the K12 transcript was mapped by 5' RACE (rapid amplification of cDNA ends) and S1 nuclease

60 ranscription start site was identified by 5' RACE (rapid amplification of complementary DNA ends).

61 criptional start sites were determined by 5' RACE analysis.

62 y and late transcripts were identified by 5' RACE and primer walking techniques.

63 ha were mapped within the third intron by 5' RACE and S1 nuclease protection.

64 transcript initiation sites identified by 5' RACE is located 159 nucleotides upstream of the putative

65 onic promoter of miR-9-5 was validated by 5' RACE PCR.

66 ptional initiation site was identified by 5' RACE techniques using total RNA from NIH 3T3 cells.

67 This was followed by sequencing of cloned 5' RACE products and of products re-amplified from excised

68 We employed 5' RACE to isolate the 5' end of the fusion transcript and

69 ed by rapid amplification of 5' cDNA end (5' RACE), RT-PCR analysis and genome sequence analyses.

70 r to rapid amplification of 5' cDNA ends (5' RACE) for HIV-1 RNA and quantitative reverse transcripta

71 and 5' rapid amplification of cDNA ends (5' RACE) to be located 25 nt upstream of the ATG in exon 1.

72 and rapid amplification of 5' cDNA ends (5' RACE), we have cloned three previously unrecognized endo

73 The classical scheme for 5' RACE described here is simple, suffices in many instance

74 led with library screening and a modified 5' RACE-PCR strategy, resulted in the identification and ch

75 ys, and independently with inverse PCR of 5' RACE clones, common mRNA initiation sites were identifie

76 By means of 5' RACE cloning methodology, a novel putative full-length h

77 The 3000 BP upstream of 5' RACE product were subcloned into a pGL3 luciferase repor

78 Interestingly, analysis of 5' RACE products for antisense transcripts and the GenBank

79 The approach was based on 5' RACE with [32P]-labelled primers and separation of produ

80 combination of reverse transcription-PCR, 5' RACE, and genomic library screening was used to clone th

81 genomic structure of BMPR1A, we performed 5' RACE from lymphoblastoid cell lines and normal colon tis

82 We performed 5' RACE on mRNA isolated from murine chondroprogenitor cell

83 le in mediating tumor regression and that 5' RACE analysis may provide an important tool for the anal

84 The 5' RACE analysis of the human testis mRNA revealed the exis

85 f the sequence variation defined from the 5' RACE products.

86 with yeast or human DBR1 enzyme prior to 5' RACE and qRT-PCR.

87 rt sites of 69 rpoH-dependent genes using 5' RACE (5' rapid amplification of cDNA ends), which allowe

88 Using 5' RACE (rapid amplification of cDNA ends) analysis, multip

89 rame (ORF) of 3.5 kb was elucidated using 5' RACE and genomic sequence.

90 upstream coding region was obtained using 5' RACE methods.

91 e 5' end of the murine Rad51l2 cDNA using 5' RACE technique as well as by sequencing the genomic regi

92 MS1 proximal promoter, initially by using 5' RACE to map transcription start sites.

93 Using 5' RACE we have now identified a novel BCL-X non-coding exo

94 Using 5' RACE, two transcription initiation sites were identified

95 Using 5' RACE, we have isolated four additional exons of the mu o

96 Using 5' RACE, we identified three promoters, designated A, B and

97 Using 5' RACE, we identified three transcription start sites and

98 rt sites in human MTHFR were mapped using 5' RACE.

99 5' -RACE and primer extension identified a long 5' -untransl

100 5'RACE followed by functional promoter analysis in multipl

101 cer-testis Ag NY-ESO-1 were cloned using a 5'RACE method from RNA isolated from a CTL generated by in

102 Bioinformatic and 5'RACE (rapid amplification of cDNA ends) analyses of the

103 RT-PCR and 5'RACE experiments confirm the presence of four additional

104 The results of RT-PCR and 5'RACE suggest that only PRB is inactivated, although PRA

105 results obtained using global RNA-Seq and 5'RACE.

106 H1 and vnfH transcriptional start sites by 5'RACE (5' rapid amplification of cDNA ends) revealed that

107 DNA sequence analysis of cDNA generated by 5'RACE from CSF1R coding sequences identified a novel fusi

108 ' rapid amplification of cDNA ends method (5'RACE), and immunohistochemical staining.

109 Rapid amplification of 5'-cDNA ends-PCR (5'RACE-PCR) revealed at least three novel forms of the unt

110 o alternative promoter was observed by RLM-5'RACE PCR and reverse transcriptase PCR analyses during e

111 amplification of 5' cDNA ends by PCR (RLM-5'RACE PCR) analysis of C. cellulovorans RNA identified a

112 Additionally, sequence analysis of the 5'RACE-PCR products revealed multiple transcriptional star

113 Using 5'RACE, we demonstrate that Giardia promoters are a source

114 in vitro DNA-binding assays combined with 5'RACE, that BrlR binds to its own promoter, likely via a

115 5'-RACE (rapid amplification of cDNA ends) data indicate th

116 5'-RACE analysis defined transcript start sites for sbi, ef

117 5'-RACE analysis of mouse embryo cDNA targeting five candid

118 5'-RACE identified p53 mRNA transcribed from a novel start

119 5'-RACE mapped the transcription start site for one of the

120 d several alternative splice sites with a 5'-RACE approach.

121 rmed by RNA-Sequencing, and extended by a 5'-RACE assay and Northern blotting, showing that meiotic c

122 A 5'-RACE study determined the transcriptional start site of

123 This prompted additional 5'-RACE experiments that established a second, TATA box-con

124 Database analysis and 5'-RACE (rapid amplification of cDNA ends) identified a 419

125 RT-PCR and 5'-RACE analysis revealed that cdr and bb0729 are co-transc

126 ive RT-PCR and used for Northern blot and 5'-RACE analysis.

127 scripts produced from the dnaK operon and 5'-RACE mapped 5' termini of multiple dnaK transcripts with

128 RT-PCR and 5'-RACE mapping showed that the 5' UTR has a length of 1.94

129 ere cloned from a skin library by 3'- and 5'-RACE reactions.

130 cDNA library screening and 5'-RACE revealed that the major transcript encoded an open-

131 ning strategy (RT-PCR followed by 3'- and 5'-RACE) to clone from Y-organs of the blue crab (Callinect

132 enes by using a combination of RT-PCR and 5'-RACE.

133 cleavage of a subset of these targets by 5'-RACE analysis.

134 anscriptional start of human CRLR cDNA by 5'-RACE and cloned the proximal 5'-flanking region of the g

135 by comparing the repertoires generated by 5'-RACE PCR and multiplex PCR.

136 tes of all three genes were determined by 5'-RACE revealing large leader sequences for each transcrip

137 Transcript analysis by 5'-RACE suggested a mechanism for allelic differences: an o

138 d associated promoter (P1), was mapped by 5'-RACE to a region 19 kb upstream of the ZFP106 translatio

139 l cDNA amplification strategy followed by 5'-RACE, we have identified several gene products whose exp

140 nt upstream of the ATG initiation site by 5'-RACE.

141 Rapid amplification of 5'-cDNA ends (5'-RACE) analysis demonstrated exclusive use of the CBS -1b

142 5'-rapid amplification of cDNA ends (5'-RACE) and computational analyses were used to identify c

143 Rapid amplification of 5'-cDNA ends (5'-RACE) and reverse transcription-PCR assays identified sh

144 Rapid amplification of cDNA 5'-ends (5'-RACE) identified additional upstream exon 1 sequence tha

145 and 5'-rapid amplification of cDNA ends (5'-RACE) revealed five major ADAR1 transcriptional start si

146 Rapid amplification of cDNA ends (5'-RACE) was used to isolate the CDT6 promoter from an adap

147 cs analysis, rapid analysis of cDNA ends (5'-RACE), and reverse transcription coupled with qPCR using

148 ing 5' random amplification of cDNA ends (5'-RACE), and the binding sites for purified HlyU were disc

149 ing rapid amplification of the cDNA ends (5'-RACE), we identified one transcription start site (TSS).

150 entary deoxyribonucleic acid (cDNA) ends (5'-RACE).

151 ngest 5'-untranslated region derived from 5'-RACE and apparently generated by the distal promoter has

152 Here we report the isolation by genomic 5'-RACE PCR and in vitro analysis of the mouse PIASgamma pr

153 oth endothelial cells and liver; however, 5'-RACE analysis (rapid amplification of cDNA ends) identif

154 We obtained evidence by modified 5'-RACE for a MYB mRNA cleavage product guided by miR828 in

155 RNA, whose cleavage was shown by modified 5'-RACE.

156 question, we conducted deep sequencing of 5'-RACE products of the Igh repertoire in pro-B cells, ampl

157 he neuronal channel SCN8A, we carried out 5'-RACE (rapid amplification of cDNA ends) with RNA from hu

158 We also performed 5'-RACE experiments on 62 novel regions, and 76% of the reg

159 Subsequent 3'-RACE, 5'-RACE and high fidelity PCR were then used to produce a f

160 eight were further confirmed with the RLM 5'-RACE experiments.

161 uently full-length cDNA was cloned by the 5'-RACE (rapid amplification of cDNA ends) technique and se

162 The 5'-RACE analysis of RARbeta2 mRNA in these cells demonstrat

163 , both the RNase protection assay and the 5'-RACE assay detected endogenous pim-1 transcripts with sh

164 d" antibody repertoires by sequencing the 5'-RACE PCR products of B-cell transcripts from IAVI donor

165 By using 5'-RACE, alignment of EST sequences, and a luciferase-repor

166 o be mediated by an RNAi mechanism, using 5'-RACE.

167 s in vitro and murine enterocytes in vivo.5'-RACE identified two novel exons, 1A and 1B, which encode

168 Using Primer walking, 5'-RACE PCR and in silico analyses, we characterized nine J

169 Additional RACE and Northern analysis revealed that at least five d

170 end-polymerase chain reaction amplification (RACE-PCR).

171 Reverse transcription-PCR amplification and RACE were used to acquire the former menthone:(-)-(3R)-m

172 Reverse transcription-PCR amplification and RACE were used to acquire the remaining 5'-sequence from

173 which 6 were confirmed by northern blot and RACE.

174 This work demonstrates Raman-DIP and RACE are effective culture-independent approach for rapi

175 was obtained and verified through RT-PCR and RACE (rapid amplification of cDNA ends).

176 The gene was amplified by RT-PCR and RACE of the poly(A)+RNA isolated from the mouse melanoma

177 s of this novel gene using interexon PCR and RACE technologies.

178 ction of oligonucleotide primers for PCR and RACE-derived cDNAs from which the complete sequence of f

179 novel sequences by using degenerate PCR and RACE.

180 We apply RACE-Seq to 398 human lncRNA genes in seven tissues, lea

181 orporating other transcriptomic data such as RACE, CAGE, and EST into its model to further increase i

182 yosuroides hydrolase (Amgdsh1) was cloned by RACE-PCR and expressed in the yeast Pichia pastoris as a

183 sequence of the DCAL-1 gene was confirmed by RACE-PCR; however, based on sequence alignment with geno

184 All corresponding cDNAs were confirmed by RACE.

185 using bioinformatic predictions, followed by RACE, RT-PCR, and sequencing.

186 We then cloned the novel gene by RACE-PCR, and analysis of the deduced cDNA sequence foun

187 ons of the mouse OR genes were identified by RACE-PCR.

188 ouse ovarian adapter-ligated cDNA library by RACE-PCR, and a unique 2043-bp open reading frame was de

189 s or siRNA and analyzed cleavage products by RACE.

190 ate Control Versus Electrical Cardioversion (RACE) trials that anticoagulation should not be disconti

191 Current RACE methods often produce a high background of nonspeci

192 e extremely GC-rich sequences, using current RACE methods.

193 nfarction in Carolina Emergency Departments (RACE) project, transported via emergency medical service

194 Here we describe RACE-Seq, an experimental workflow designed to address t

195 Many different RACE methods have been developed to meet various require

196 Raman activated cell ejection (RACE) was applied to isolate single AMR bacteria for the

197 5' Random amplification of cDNA ends (RACE) analyses identified two distinct promoters, p1 and

198 5' rapid amplification of cDNA ends (RACE) analyses of RNAs prepared from G50DblKo and wild-t

199 3'-Rapid amplification of cDNA ends (RACE) analyses of the terminated RNA products allowed pr

200 5' rapid amplification of cDNA ends (RACE) and deletion analysis were used to identify the di

201 erns using rapid amplification of cDNA ends (RACE) and full-length cDNA sequencing, revealed four ind

202 such as 5'-Rapid Amplification of cDNA Ends (RACE) and inverse polymerase chain reaction (PCR).

203 ations, 3' rapid amplification of cDNA ends (RACE) and polymerase chain reactions (PCR) were performe

204 luding the rapid amplification of cDNA ends (RACE) and tiling array technologies that was used to fur

205 ted by the rapid amplification of cDNA ends (RACE) approach.

206 5' and 3' rapid amplification of cDNA ends (RACE) experiments and findings of novel splicing events

207 ction, and rapid amplification of cDNA ends (RACE) experiments indicate the presence of multiple tran

208 m adapting rapid amplification of cDNA ends (RACE) for large-scale structural transcript annotation.

209 ies and by rapid amplification of cDNA ends (RACE) from liver, spleen and lung RNA.

210 sis and 3' rapid amplification of cDNA ends (RACE) in placenta confirmed the existence of distal intr

211 Rapid amplification of cDNA ends (RACE) is widely used to determine the 5'- and 3'-termina

212 mRNA using rapid amplification of cDNA ends (RACE) PCR as long as part of the mRNA sequence is known;

213 sults from rapid amplification of cDNA ends (RACE) PCR suggest that there are multiple transcriptiona

214 t using 5' rapid amplification of cDNA ends (RACE) PCR.

215 3' rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR) from bone marrow m

216 The rapid amplification of cDNA ends (RACE) procedure is a widely used PCR-based method to clo

217 5'- and 3'-rapid amplification of cDNA ends (RACE) product and assembling the sequences, we generated

218 5'- and 3'-Rapid Amplification of cDNA Ends (RACE) revealed IGH/CHST11 as well as CHST11/IGH fusion R

219 have used rapid amplification of cDNA ends (RACE) to identify multiple transcription initiation and

220 Rapid amplification of cDNA ends (RACE) was performed on RNA isolated from human RPE cells

221 Rapid amplification of cDNA ends (RACE) was used to obtain potentially full-length transcr

222 method of rapid amplification of cDNA ends (RACE) was used to obtain their cDNA sequences from 11 cD

223 ned using random amplification of cDNA ends (RACE), and promoter regions were compared with orthologu

224 encing, 3' rapid amplification of cDNA ends (RACE), and tiled microarray analyses.

225 (RT-PCR), rapid amplification of cDNA ends (RACE), and transient expression of minigene constructs.

226 ed with 5' rapid amplification of cDNA ends (RACE), in vitro transcription assays, real-time quantita

227 etected by rapid amplification of cDNA ends (RACE), primer extension, and ribonuclease protection ass

228 Using rapid amplification of cDNA ends (RACE), reverse-transcription polymerase chain reaction (

229 , using 5' rapid amplification of cDNA ends (RACE), two transcriptional start sites (TSSs) and two pu

230 Using 3' rapid amplification of cDNA ends (RACE), we mapped the 3' end of the N and NSs mRNAs, show

231 y using 5' rapid amplification of cDNA ends (RACE), we mapped two HPV18 transcription start sites (TS

232 Rapid amplification of cDNA ends (RACE)-PCR extension of expressed sequence-tag sequences

233 solated by rapid amplification of cDNA ends (RACE).

234 loning and rapid amplification of cDNA ends (RACE).

235 scripts by rapid amplification of cDNA ends (RACE).

236 T-PCR) and rapid amplification of cDNA ends (RACE).

237 y by using rapid amplification of cDNA ends (RACE).

238 ermined by rapid amplification of cDNA ends (RACE).

239 ssed by 5' Rapid Amplification of cDNA Ends (RACE).

240 pid amplification of complementary DNA ends (RACE), chemical inhibition experiments, and genetic disr

241 pid amplification of complementary DNA ends (RACE), two transcriptional start sites were identified.

242 repetitive alveolar collapse and expansion" (RACE).

243 the Real-time Accurate Cell-shape Extractor (RACE), a high-throughput image analysis framework for au

244 We describe here an improved RACE procedure using circular cDNA templates and demonst

245 Thus, the term "collapse" in RACE refers to a visibly obvious collapse of the alveolu

246 by the development of alveolar instability (RACE) and the increase in alveolar size at peak inspirat

247 We furthermore integrated RACE with our framework for automated cell lineaging and

248 We have used RNA ligase mediated RACE and in silico analyses to locate two sets of transc

249 tokine termed IL-17D was cloned using nested RACE PCR.

250 kine, termed IL-17F, was cloned using nested RACE PCR.

251 'New RACE' (rapid amplification of cDNA ends) PCR is a method

252 Thus, we developed a new RACE method that can be used for this purpose.

253 Although 'new RACE' can also be used to amplify 3' ends, only the prot

254 In 'new RACE', an anchor is ligated to the 5' end of the mRNA be

255 of effector action, the requirement for NON-RACE-SPECIFIC DISEASE RESISTANCE1 (NDR1) is shared.

256 Arabidopsis (Arabidopsis thaliana) NON-RACE-SPECIFIC DISEASE RESISTANCE1 (NDR1), a plasma membr

257 We obtained RACE data for approximately two-thirds of the examined t

258 Sequence analysis of RACE products showed that the truncation is due to a sin

259 We demonstrate the generality of RACE by extracting cell-shape information from entire Dr

260 By means of RACE PCR, three full-length cDNAs not reported previousl

261 l workflow designed to address this based on RACE (rapid amplification of cDNA ends) and long-read RN

262 ing 5' rapid amplification of cDNA ends-PCR (RACE-PCR), and strong sigma(54) and sigma(70) consensus

263 by PCR-rapid amplification of cDNA ends (PCR-RACE).

264 diated Rapid Amplification of cDNA Ends (PPM-RACE).

265 tion of cDNA ends polymerase chain reaction (RACE PCR).

266 ementary DNA ends-polymerase chain reaction (RACE-PCR) on patient RNA, rabaptin-5 was identified as a

267 Gene reporter, RACE, and chemical inhibitor studies demonstrated that t

268 from Hydra vulgaris using 3'- and 5'- (RLM) RACE approaches.

269 Experiments using 5'RLM-RACE demonstrated that the genes EpSPL1, 2, 3, 4, 7, 9,

270 transcriptome profiling combined with 5'-RLM-RACE analysis in transgenic plants confirmed that amiRNA

271 In the current work, we have utilized 5'-RLM-RACE to examine the influence of CGG repeat number on th

272 diated rapid amplification of cDNA ends (RLM-RACE) and RT-PCR to identify four transcription start si

273 diated rapid amplification of cDNA ends (RLM-RACE) between -61 and -32 bp from the translation initia

274 diated rapid amplification of cDNA ends (RLM-RACE) PCR analysis indicated that the single transcripti

275 diated rapid amplification of cDNA ends (RLM-RACE) reads, and 50,000 cap-trapped expressed sequence t

276 their 5' and 3' UTR were amplified using RLM-RACE.

277 e corrected proactively with our large-scale RACE platform.

278 otting, reverse transcription-PCR, and SMART-RACE analyses suggest that the dlt transcript begins 250

279 med systematic series of pseudogene-specific RACE analyses.

280 lve altered alveolar mechanics, specifically RACE and alveolar overdistension.

281 sted gene-specific primer and the 3' or 5' T-RACE primer results in specific amplification of cDNA en

282 Overall, we show that RACE-Seq is an effective tool to annotate an organism's

283 The RACE amplification technology was used on a novel CYP3A-

284 The RACE II (Rate Control Efficacy in Permanent Atrial Fibri

285 The sequences of the RACE sorted cells indicate that they were potential huma

286 In addition, high-throughput RACE successfully extended the 5' and/or 3' ends of >60%

287 sing this targeted approach, high-throughput RACE, we revealed numerous transcripts including many un

288 associated with the 5'-ends of at least two RACE products.

289 Using RACE PCR, reverse transcription-PCR (RT-PCR) and RNA-seq

290 Using RACE procedures, we cloned and sequenced the complete 4.

291 Using RACE, we automatically reconstructed cellular-resolution

292 sis of the 5'- and 3'-ends of the mRNA using RACE analysis determined that the ADH4 mRNA in C57BL/6 m

293 th cDNAs of each product were obtained using RACE-PCR.

294 nfirmed its 3' end nucleotide sequence using RACE-PCR.

295 of supporting evidence and tested them using RACE-PCR.

296 se results show that direct validation using RACE-PCR can be an important component of genome-wide va

297 simple, suffices in many instances in which RACE is needed and can be performed in 1-3 days.

298 thology, whereas lung tissue from areas with RACE mechanics demonstrated alveolar collapse, atelectas

299 RT-PCR sequencing in combination with RACE on ab initio gene predictions could be used to defi

300 ing strand-specific RT-PCR complemented with RACE and FISH.