ライフサイエンスコーパス: RAPD

1 RAPD analysis demonstrated a 1.4-kb band in all O19 stra

2 RAPD and PFGE showed that the 24 strains were a genetica

3 RAPD as measured in log units significantly correlated w

4 RAPD fingerprints obtained from primary culture plate co

5 RAPD fingerprints were much more informative than the pl

6 RAPD genotyping significantly correlated with resistance

7 RAPD marker analysis is more reliable and provides highe

8 RAPD markers cosegregated into 12 different S. brevidens

9 RAPD markers showed an overall bias toward vitripennis a

10 RAPD size correlated significantly with both RNFL thickn

11 RAPD type A was also isolated from the rubber liners of

12 RAPD was measured by the swinging flashlight method usin

13 RAPD-PCR demonstrated identical patterns among M. chimae

14 RAPD-PCR polymorphisms at 57 presumptive loci were used

15 RAPD-PCR polymorphisms were used to examine breeding str

16 RAPD-SSCP analysis revealed segregation of codominant al

17 RAPDs indicate that L. robustum ssp. walkeri in Sri Lank

18 in A aygpti, 94 markers were mapped with 10 RAPD primers in five weeks.

19 etailed map was constructed that includes 10 RAPDs.

20 ChxA locus (cycloheximide resistance) and 11 RAPDs (randomly amplified polymorphic DNAs).

21 n B. hebetor, 79 markers were mapped with 12 RAPD primers in six weeks; in A aygpti, 94 markers were

22 by the RAPD fingerprinting method alone, 14 RAPD subtypes were revealed.

23 quence-tagged site markers was initiated: 18 RAPD markers were cloned and sequenced, and single-stran

24 Polymorphisms were scored at 47-48 RAPD loci in each species.

25 Genotypes were also scored at 57 RAPD-SSCP loci, 5 (TAG)(n) microsatellite loci, and 6 se

26 The map includes 71 RAPD markers and one phenotypic marker, blonde.

27 ntains 79 markers (six microsatellite and 73 RAPD markers) in 21 linkage groups and spans over 953.1

28 y nullisomic mapping, does show linkage to a RAPD not yet assignable to chromosomes by nullisomic map

29 AFLP-, RAPD-, and RFLP-derived markers were used to saturate th

30 A total of 347 loci (AFLPs, RAPDs, and protein-coding loci) were mapped using an int

31 An RAPD was observed in 9% to 82% of patients with glaucoma

32 ERIC-PCR revealed 21 and RAPD-PCR revealed 18 distinct patterns of isolates with

33 between South American classes 5 and 6, and RAPD-PCR showed 18 distinct genetic fingerprints in 20 i

34 0.320), demonstrating the value of AFLP and RAPD analyses in the characterization of disease-causing

35 Both AFLP and RAPD separated the isolates into two distinct groups, bu

36 c linkage map composed of 841 SSR, AFLP, and RAPD markers and phenotypic data from 310 progeny were u

37 on of Fst estimates across both allozyme and RAPD loci.

38 ation of bulked segregant analysis (BSA) and RAPD techniques.

39 or a close relative of CGA (by ERIC2 PCR and RAPD analysis, respectively) and yielded a positive assa

40 PFGE and RAPD fingerprinting are best suited to addressing small-

41 r of the methods was variable, with PFGE and RAPD typing having a higher index of discrimination than

42 ethods employed, including PT, RT, PFGE, and RAPD analysis.

43 presence of DNase, antibiotic resistance and RAPD profiles indicated that transfer was unidirectional

44 . coli with similar levels of resistance and RAPD-genotypes on days 0 and 42.

45 A total of 125 RFLP and RAPD markers were mapped into 117 different loci on nine

46 Ribotyping and RAPD clearly demonstrated the household transmission of

47 ere indistinguishable by both ribotyping and RAPD.

48 were less discriminatory than ribotyping and RAPD.

49 d M. szulgai strain with a gene sequence and RAPD pattern identical to those of the pseudoepidemic st

50 CYP51A sequencing and RAPD genotyping was performed.

51 e as dominant alleles, (2) that genotypes at RAPD loci are in Hardy-Weinberg proportions, (3) identit

52 Relationships between RAPD and RNFL thickness difference/ratio between the two

53 Both RAPD and ERIC genotyping methods had no correlation with

54 shown within each of these 11 pairs by both RAPD-PCR and AFLP analyses.

55 suming (1) that genomic regions amplified by RAPD-PCR segregate as dominant alleles, (2) that genotyp

56 dom association between loci demonstrated by RAPD analysis and MLEE provide evidence for strong linka

57 The changes detected by RAPD-PCR and AFLP indicate that genetic drift occurs wit

58 f one of the three clusters discriminated by RAPD analysis, MLEE, and Ca3 fingerprinting, supporting

59 concordant with the genotypes identified by RAPD analysis, MLEE, and Ca3 Southern hybridization, res

60 ith a mixture of seven strains identified by RAPD-polymerase chain reaction, H. pylori Lewis expressi

61 breast tissues from the same individuals by RAPD (random amplified polymorphic DNA)/AP-PCR (arbitrar

62 olates closely resembled chicken isolates by RAPD and PFGE analysis.

63 that were of low intensity were observed by RAPD analysis, making interpretation more difficult.

64 Thirty types were observed by RAPD analysis.

65 rogram prepared from the results obtained by RAPD analysis, the isolates from the United States and E

66 were generally different from each other by RAPD analysis.

67 he large number of polymorphisms revealed by RAPD-PCR allowed the distribution of FST and linkage dis

68 mportant Aspergillus species was screened by RAPD-PCR to identify section- or species-specific amplic

69 , and inconclusive when comparing strains by RAPD-PCR.

70 PFGE gave evaluable patterns when studied by RAPD-PCR, with isolate clustering being consistent with

71 re resolved into three different subtypes by RAPD analysis.

72 early distinguished from Indian Ligustrum by RAPDs.

73 The process of converting RAPD markers to sequence-tagged site markers was initiat

74 study demonstrates the efficacy of coupling RAPD and HRS for a rapid and efficient screening of the

75 fluences the relative afferent pupil defect (RAPD).

76 cause a relative afferent pupillary defect (RAPD).

77 entified similar subtle genomic differences, RAPD analysis provided only 77% of the detail provided b

78 However, the differentiated RAPDs and mtDNA were concordant in dividing the populati

79 A total of 62 distinct RAPD types were categorized from the five genera studied

80 th data from random amplified polymeric DNA (RAPD) and Penner serotyping analyses.

81 re done by random amplified polymorphic DNA (RAPD) analysis and amplified fragment length polymorphis

82 s based on random amplified polymorphic DNA (RAPD) analysis and multilocus enzyme electrophoresis (ML

83 gens using random amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFG

84 Randomly amplified polymorphic DNA (RAPD) analysis generated DNA profiles that clustered the

85 obes using random amplified polymorphic DNA (RAPD) analysis revealed genetic similarities and differe

86 , based on random amplified polymorphic DNA (RAPD) analysis was developed for its efficient and accur

87 C2 PCR and random amplified polymorphic DNA (RAPD) analysis, a PCR assay incorporating the new primer

88 bjected to random amplified polymorphic DNA (RAPD) analysis, and the data were analysed phenetically.

89 Randomly amplified polymorphic DNA (RAPD) analysis, multilocus enzyme electrophoresis (MLEE)

90 g methods: random amplified polymorphic DNA (RAPD) analysis, sequence-specific DNA primer (SSDP) anal

91 ugh random amplification of polymorphic DNA (RAPD) analysis.

92 AFLP), and random amplified polymorphic DNA (RAPD) analysis.

93 at couples Random Amplified Polymorphic DNA (RAPD) and a high-resolution capillary electrophoresis sy

94 ysed using random amplified polymorphic DNA (RAPD) and mtDNA RFLPs markers.

95 other by randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism analy

96 CR-based randomly amplified polymorphic DNA (RAPD) assay as well as to pulsed-field gel electrophores

97 an 810-bp random amplified polymorphic DNA (RAPD) band in the F1 and backcross generations of a Sile

98 of eight randomly amplified polymorphic DNA (RAPD) fingerprint types from Calcutta, India, that were

99 A random amplified polymorphic DNA (RAPD) fingerprinting method has been developed to differ

100 Randomly amplified polymorphic DNA (RAPD) fingerprints generated from a single primer correc

101 mined with randomly applied polymorphic DNA (RAPD) markers in a second backcross population derived f

102 f the nine randomly applied polymorphic DNA (RAPD) markers we examined were more strongly differentia

103 s, using randomly amplified polymorphic DNA (RAPD) markers, conclusively showed that somatically comp

104 s of 122 randomly amplified polymorphic DNA (RAPD) markers, six molecular markers representing identi

105 cted using random amplified polymorphic DNA (RAPD) markers.

106 kers and randomly amplified polymorphic DNA (RAPD) markers.

107 y of the randomly amplified polymorphic DNA (RAPD) method to uncover genetic variation in this highly

108 A Randomly Amplified Polymorphic DNA (RAPD) method was developed for efficient detection of ad

109 reas the randomly amplified polymorphic DNA (RAPD) patterns of all nonpigmented strains were identica

110 s by using random amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) cloning and the TaqMan LS50B fluoro

111 PFGE by randomly amplified polymorphic DNA (RAPD) PCR, including isolates from eight nosocomial outb

112 Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Te

113 iii) the randomly amplified polymorphic DNA (RAPD) technique, and (iv) pulsed-field gel electrophores

114 and random amplification of polymorphic DNA (RAPD) type-specific differences in gluconate production,

115 inting and random amplified polymorphic DNA (RAPD) typing.

116 sis (MEE), random amplified polymorphic DNA (RAPD), and single-strand conformation polymorphism-were

117 ping (EK), random-amplified polymorphic DNA (RAPD), and the CNRE-1 DNA probe, MICs of amphotericin B,

118 sed random amplification of polymorphic DNA (RAPD), have already been utilized as powerful tools to d

119 ted by the random amplified polymorphic DNA (RAPD), multilocus enzyme electrophoresis (MLEE), and Ca3

120 ing random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE), and DNA-

121 by Random Amplification of Polymorphic DNA (RAPD)-PCR fingerprinting was used to group the genotypes

122 ared using random amplified polymorphic DNA (RAPD)-PCR typing.

123 by random amplification of polymorphic DNA (RAPD)-polymerase chain reaction (PCR).

124 (ERIC) and Random Amplified Polymorphic DNA (RAPD).

125 as random amplification of polymorphic DNA (RAPD)] and isozyme mapping resulted in the production of

126 CR-based randomly amplified polymorphic DNA (RAPD-PCR) method.

127 A, and IS605; random arbitrarily primed DNA (RAPD)-PCR and amplified fragment length polymorphism (AF

128 LPs) and randomly amplified polymorphic DNA (RAPDs), the methods of data analysis can be split into t

129 arkers and random amplified polymorphic DNA (RAPDs).

130 E], random amplification of polymorphic DNA [RAPD], and amplified fragment length polymorphism [AFLP]

131 a genetically diverse population with eight RAPD profiles.

132 ted primer sets are identified and employed, RAPD analysis provides a simple, rapid, and powerful sub

133 ica, from Mexico to Panama, and analysed for RAPD DNA variation.

134 ncordance of the results is demonstrated for RAPD analysis, MLEE, and Southern blot hybridization wit

135 The methodologies for RAPD typing, ribotyping, and PFGE typing of C. diphtheri

136 two known, noninbred parents was scored for RAPD and AFLP markers, in order to develop a linkage map

137 anisocoria produced a significant change in RAPD from baseline (mean = 1.60 dB in the miotic eye, P

138 A total of 32decamer primers were used in RAPD analysis; 19 of them provided at least one polymorp

139 To test EstA and AcpA for linkage to known RAPD loci on their respective chromosomes, a panel of Ro

140 markers were consistent with those of linked RAPD markers.

141 specificity and the lack of codominance make RAPD markers less practical for mapping than microsatell

142 ci are employed (eg 50 loci, typical of many RAPD studies).

143 rase chain reaction-based molecular markers (RAPDs).

144 the samples were screened with eight 10-mer RAPD primers, a total of 12 polymorphic bands were detec

145 Moreover RAPD markers have been applied in order to describe thei

146 Moreover, RAPDs showed that introduced material in La Reunion has

147 nalyzed by agarose gel electrophoresis, most RAPD-PCR markers segregate as dominant alleles, reducing

148 d several weeks later was caused by multiple RAPD types, which rules out contagious transmission and

149 Nevertheless, RAPD-PCR allowed recording a relevant genotypic biodiver

150 conformation polymorphism (SSCP) analysis of RAPD markers to generate linkage maps in a haplodiploid

151 The analysis of RAPD products with HRS showed differences between the pr

152 SSCP analysis of RAPD-PCR markers offers a rapid and inexpensive means of

153 The discriminatory power of RAPD analysis was slightly lower (D = 0.971), whereas SS

154 poor postoperative outcome were presence of RAPD (p = .014), wound extending into zone III (p = 0.02

155 Better detection of RAPDs may be one way to identify persons with glaucoma.

156 With the OPZ19 RAPD primer a 1,264-bp product was amplified from all A.

157 No changes in MEE or RAPD subtypes were detected in serial isolates from any

158 ither testing on CHROMagar Candida plates or RAPD fingerprinting.

159 ne-third as many distinct subtypes as REA or RAPD analysis; the Singaporean isolates were distributed

160 to interpret than those obtained by RFLP or RAPD analysis.

161 integration genes were present in two other RAPD types.

162 of all 4 coincided with those of the parent RAPD markers.

163 ofile and random arbitrarily primed DNA PCR (RAPD-PCR) profiles of the progeny indicated that DNA tra

164 random amplification of polymorphic DNA-PCR (RAPD-PCR).

165 random amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) cloning and the TaqMan LS50B fluorogenic detec

166 Linkage analysis of AP-PCR/RAPD and isozyme markers resulted in the addition of 16

167 nal resolution and reproducibility of AP-PCR/RAPD generated markers.

168 horus gene map by linkage analysis of AP-PCR/RAPD markers in conjunction with isozyme polymorphisms s

169 Addition of 5 AP-PCR/RAPD markers to linkage group U6 containing the Tailspot

170 n randomly chosen and characterized by PFGE, RAPD, and AFLP.

171 ly chosen and subjected to analysis by PFGE, RAPD, and AFLP.

172 by a single strain of Klebsiella pneumoniae, RAPD type A, which was detected in milk from eight cows.

173 en decamer primers generated 102 polymorphic RAPD bands and pairwise distances were calculated betwee

174 phic DNA from the polymerase chain reaction (RAPD-PCR) allows efficient construction of saturated lin

175 d polymorphic DNA polymerase chain reaction (RAPD-PCR).

176 Six RAPD types and 15 PFGE patterns were identified among al

177 ast one, but not all, synteny group-specific RAPD markers.

178 We constructed a joint SSR/RAPD genetic linkage map of the Helianthus petiolaris ge

179 These findings suggest RAPD-PCR typing can distinguish unique CF isolates of S.

180 ) microsatellite loci, and 6 sequence-tagged RAPD loci.

181 PFGE with SpeI was more reproducible than RAPD and BOX-PCR fingerprinting.

182 This study demonstrates that RAPD-PCR can be used for genetic comparison of M. absces

183 We propose that RAPD and AFLP provide valuable tools for molecular typin

184 The RAPD data revealed within-section interspecies relations

185 The RAPD method appears useful for the construction of a gen

186 The RAPD profiles of four isolates obtained were again ident

187 The RAPD technique was adequate for biotype differentiation;

188 The RAPD-SCAR technique is here described, for the first tim

189 However, by the RAPD fingerprinting method alone, 14 RAPD subtypes were

190 ts minor differences were seen in either the RAPD genotype or the microsatellite allele composition d

191 All 10 primers used for the RAPD analyses can distinguish C. fetus strains of reptil

192 and Critical Care Center were involved, the RAPD assay revealed that all 20 isolates obtained from c

193 d in a shorter time, the introduction of the RAPD assay into hospital microbiology laboratories as a

194 Approximately a third of the RAPD markers analysed segregated in both the 87-25-1M x

195 the potential influence of anisocoria on the RAPD and also greater susceptibility of lightly pigmente

196 ll 25 linkage groups have been mapped on the RAPD zebrafish genetic map.

197 Since the RAPD assay could provide discriminatory potential and re

198 mmon source (the pseudoepidemic strain), the RAPD patterns of the other strains were varied.

199 Thus, our experience demonstrated that the RAPD assay is a useful and reliable tool for epidemiolog

200 electrophoretic karyotyping showed that the RAPD fingerprint was correct.

201 The main outcome measure was the RAPD, determined by computerized pupillography, at basel

202 he microsatellite-based genetic map with the RAPD map to identify markers most likely linked to the c

203 Molecular typing with the RAPD technique was useful in discriminating between pati

204 AcpA (on 3R), while unlinked to all the RAPDs assigned to chromosome 3 by nullisomic mapping, do

205 These RAPD data enabled us to pinpoint a possible source and t

206 These RAPD markers permit the PCR amplification of a DNA speci

207 This RAPD approach to S. enteritidis subtyping provided more

208 This RAPD-PCR and TaqMan assay offers promise as a nucleic ac

209 The bacterial DNA used in this RAPD protocol was obtained by boiling the bacterial samp

210 nd anthocyanin spotting (Rs)] were linked to RAPD loci, but only six of them could be placed on an ex

211 owed perfect amplification when subjected to RAPD, restriction digestion and amplification with DNA b

212 in (ctx) genes were found in isolates of two RAPD types, in each case embedded in CTXphi-like prophag

213 ntrogression in this tree was detected uisng RAPD markers.

214 First, a linkage map was produced using RAPD markers.

215 trains were isolated and characterized using RAPDs and sequencing of the almost complete 16S rRNA gen

216 .3% direct concordance with MLEE, 82.7% with RAPD analysis, and 86.2% with the Ca3 Southern hybridiza

217 No new cases with RAPD type A were observed after implementation of interv

218 However, anisocoria correlated with RAPD only in subjects with darkly pigmented irides (Pear