ライフサイエンスコーパス: RBE

1 RBE of ~1.48 in the SOBP and ~1 in the plateau were meas

2 RBE showed more effective inhibition of polyphenol oxida

3 RBE thus acts to maintain two different types of spatial

4 Both ch-19 RBE and trs elements have been shown to be essential for

5 Rep discriminate between these and the ch-19 RBE sequence?

6 Co-transfection of the pTZU6+27 RBE(apt) plasmid with HXBDeltaBgl, or pNL4-3, at a weigh

7 were observed on all substrates containing a RBE sequence, but never on DNA lacking an RBE.

8 Additionally, WT G, but not a RBE epitope mutant, could dissociate from F upon ephrinB

9 e AAV2 inverted terminal repeat or the AAVS1 RBE sequence elements neither enhances nor severely comp

10 a RBE sequence, but never on DNA lacking an RBE.

11 restingly, Rep interactions with the RBE and RBE' during nicking seem to be functionally distinct.

12 investigate the contribution of the RBE and RBE' during this process.

13 iral effect of an HIV-1 Rev-binding aptamer [RBE(apt)] could be enhanced by a ribozyme directed again

14 Although the 22-bp RBE contains the GAGC motifs that have been found in all

15 .1, EMBL accession number ) or the rat brain RBE-2 (NCX1.5, GenBank(TM) accession number ) Na(+)-Ca(2

16 The anti-HIV activity of the CMV RBE(apt) construct was non-specific, because co-transfec

17 y of linear substrates containing a complete RBE with hairpinned substrates and found that linear sub

18 We find that the concatenated RBEs can facilitate the cytoplasmic transport of viral m

19 ancies between experimental data and current RBE models.

20 emonstrate that, early in petal development, RBE represses the transcription of a suite of CIN-TCP ge

21 RABBIT EARS (RBE) encodes a C2H2 zinc finger transcriptional represso

22 The RABBIT EARS (RBE) gene has been identified as a regulator of petal de

23 criptional repressor encoded by RABBIT EARS (RBE) regulates the expression of all three miR164 genes.

24 al muscle known as the repeated-bout effect (RBE).

25 Relative biological effectiveness (RBE) factors for surrogate cancer endpoints in cell cult

26 Relative biological effectiveness (RBE) is the ratio of the effects generated by high LET r

27 amage and relative biological effectiveness (RBE) of a clinically relevant antiproton beam.

28 , and the relative biological effectiveness (RBE) of carbon-ion beams over X-rays.

29 reported relative biological effectiveness (RBE).

30 in their Relative Biological Effectiveness (RBE).

31 n, 50 Gy (relative biological effectiveness [RBE]) versus 70 Gy (RBE), on visual outcomes was analyze

32 Both a Rep DNA binding element (RBE) and a nicking site essential for AAV replication pr

33 An AAV Rep binding element (RBE) and terminal resolution site (trs) identical to the

34 e high affinity Rev protein binding element (RBE) has been determined at 2.1-A resolution.

35 pete with the wild-type Rev-binding element (RBE) in vitro, it was not known whether they would be ab

36 The p5 Rep binding element (RBE) is essential in RMSSI and Rep-dependent replication

37 es based on the minimal Rev Binding Element (RBE) of HIV-1.

38 Two Rev binding element (RBE) RNA oligonucleotides were used as model systems in

39 ide which contained the Rep binding element (RBE) within the A stem of the TR.

40 sequence elements, the Rep binding element (RBE), a small palindrome that comprises a single tip of

41 ence composed of an AAV Rep binding element (RBE), a spacer, and a nicking site.

42 ear 22-bp sequence, the Rep binding element (RBE), that is within both the terminal repeat (TR) and t

43 hesized concatemers of Rev-binding elements (RBEs) that fold to form multiple, discrete, high-affinit

44 e of several redundant Rep binding elements (RBEs) within the p5 promoter or within the terminal repe

45 al entry, and its receptor binding-enhanced (RBE) epitope was temperature-dependent, suggesting that

46 t that a new species, Roussin's Black Ester (RBE), may be formed, in which one or more of the sulfide

47 onstrated that AAV4 Rep68 bound the expanded RBE with a sixfold-greater affinity than the human RBE.

48 with the guanine nucleotides of the expanded RBE, thus providing a biochemical basis for the increase

49 ays to determine the effect of this expanded RBE sequence on the Rep-RBE interaction and AAV targeted

50 nd commercially defatted rice bran extracts (RBE and CDRBE) were evaluated for their ability to inhib

51 dictated by differential Rep affinities for RBE sites.

52 uble mutants supports an additional role for RBE in organ separation.

53 -thirds (70.4%) of patients receiving 50 Gy (RBE) and nearly half (45.1%) of patients receiving 70 Gy

54 A radiation dose reduction from 70 to 50 Gy (RBE) did not seem to increase the proportion of patients

55 ly half (45.1%) of patients receiving 70 Gy (RBE) retained 20/200 or better vision 5 years after trea

56 iological effectiveness [RBE]) versus 70 Gy (RBE), on visual outcomes was analyzed.

57 On the other hand, RBE' contacts seem to be required primarily for TR unwin

58 base damage, which contributes to the higher RBE of high LET radiation-induced cell killing.

59 -1 cells, functions analogously to the human RBE and provide further evidence for a developing model

60 th a sixfold-greater affinity than the human RBE.

61 Recently, identical RBE sequences have been identified at other locations in

62 odel system which found that ferulic acid in RBE and p-coumaric acid in CDRBE were active in enzymati

63 Five phenolic compounds in RBE and CDRBE (protocatechuic acid, vanillic acid, p-cou

64 ally, two Rep mutants that were deficient in RBE binding and transactivation but positive for p5 repr

65 During the early phase of petal initiation, RBE regulates a microRNA164-dependent pathway that contr

66 as also shown to be predictive of carbon ion RBE (R (2) = 0.77).

67 nd others demonstrated that the cell-killing RBE is involved in the interference of high LET radiatio

68 C cells, but not KRAS-mutant cells, show low RBE.

69 erature-dependent, suggesting that the Mab45 RBE epitope on G may be involved in triggering F.

70 The Mab45 RBE epitope was mapped to the base of the globular domai

71 targeted integration; specifically, how many RBE sequences are in the human genome?

72 We also show that the role of RBE in sepal and petal development is mediated in part t

73 These results indicate that one role of RBE is to fine-tune miR164 expression to regulate the CU

74 In the development of second-whorl organs, RBE acts in the same pathway and downstream of UNUSUAL F

75 for ch-19 is not distinct compared to other RBEs in the human genome when utilizing naked DNA.

76 In the absence of the TR, the p5 RBE and the p19 Sp1 site at position -50 are essential f

77 ss this possibility, we replaced both the p5 RBE and the p19 Sp1 site with GAL4 binding sites.

78 is suggested that the primary role of the p5 RBE and the p19 Sp1 sites was to act as a scaffold for b

79 d that in the presence of adenovirus, the p5 RBE represses p5 transcription while the RBE in the TR a

80 However, both the TR RBE and the p5 RBE transactivate the p19 and p40 promoters.

81 acetyltransferase constructs in which the p5 RBE was inserted at different locations upstream or down

82 replication; however, replacement of the p5 RBE with either the AAV2 inverted terminal repeat or the

83 Rep repression of p5 is specific for the p5 RBE, as other p5 promoter elements do not support this a

84 The fact that the p5 RBE-Rep complex can transactivate p19 and p40 while repr

85 p are present to interact with all potential RBE sites.

86 To understand how the Rep-RBE complex within p5 activated p19, we considered the p

87 ect of this expanded RBE sequence on the Rep-RBE interaction and AAV targeted integration.

88 to determine the characteristics of the Rep-RBE interaction.

89 ticulum-retained mutants, CsA did not rescue RBE-2/F913-->Stop, an endoplasmic reticulum-retained fun

90 or gene therapy reagents that target the Rev:RBE interaction.

91 ar factors do not directly influence the Rev:RBE interaction.

92 Evaluation of stilbene-containing RNA RBE sequences of varying length for their ability to bin

93 addition of the anti-env ribozyme to the RSV RBE(apt) did not enhance its antiviral activity.

94 The RSV RBE(apt) plasmid co-transfected with either HIV clone, a

95 However, the simian RBE is expanded, having five perfect directly repeated G

96 In vivo, integration targeted to the simian RBE was demonstrated by PCR analysis of latently infecte

97 these experiments establish that the simian RBE, identified in CV-1 cells, functions analogously to

98 n of footprints on both the human and simian RBEs revealed nearly identical protection; however, MI a

99 , no such changes were detected with soluble RBE epitope mutants or short-stalk G mutants.

100 tagenesis analysis suggested that the A-stem RBE contains only a single Rep binding site rather than

101 to efficient nicking at the trs: the A-stem RBE, the secondary structure element which consists of t

102 ive to sequence manipulations of the p5 TATA/RBE/YY1+1 core structure in a manner that reflects the f

103 Furthermore, we demonstrate that RBE directly interacts with the promoter of MIR164c and

104 utations on petal lamina growth suggest that RBE is also required to regulate later developmental eve

105 The RBE acted like a repressor element at most positions in

106 The RBE by itself or in combination with the YY1+1 initiator

107 The RBE(apt) and anti-env ribozyme genes were inserted into

108 of repression increased dramatically as the RBE was inserted closer to the p19 promoter.

109 the G-G base pair is required to assume the RBE conformation present in the NMR model of the complex

110 in the NMR model of the complex between the RBE and the Rev peptide.

111 model that proposes individual roles for the RBE and the spacer and nicking site elements.

112 e Rev responsive, it may be possible for the RBE to readily mutate in response to drugs or gene thera

113 Finally, since sequences distinct from the RBE are found to be Rev responsive, it may be possible f

114 termine if ECM remodeling is involved in the RBE, we conducted a second study by use of a repeated-bo

115 d that ephrinB2 binding to WT G, but not the RBE-epitope mutants, could trigger F.

116 Here we investigate the contribution of the RBE and RBE' during this process.

117 al, with nucleotides at the periphery of the RBE having the least effect on binding affinity and thos

118 terations in the polarity or position of the RBE relative to the trs greatly inhibit Rep nicking.

119 ents in which two adjacent base pairs of the RBE were substituted simultaneously with nucleotides tha

120 Thus, the effect of the RBE(apt) was strongly dependent on the promoter of the t

121 w and was independent of the presence of the RBE(apt).

122 tripartite cleavage signal comprised of the RBE, the RBE', and the trs.

123 Rev-responsive element (RRE) in place of the RBE.

124 te cleavage signal comprised of the RBE, the RBE', and the trs.

125 ey would be able to functionally replace the RBE in vivo.

126 We concluded that the RBE by itself was not a conventional upstream activation

127 ur data indicate that Rep is tethered to the RBE in a specific orientation during trs nicking.

128 e p19 Rep proteins, which do not bind to the RBE, can eliminate repression of the p5 promoter by Rep7

129 ve activity that appear to contribute to the RBE.

130 p5 RBE represses p5 transcription while the RBE in the TR activates p5.

131 Interestingly, Rep interactions with the RBE and RBE' during nicking seem to be functionally dist

132 Rep contacts with the RBE appear necessary for both the DNA helicase and trs c

133 lowing specific nucleotide contacts with the RBE' and directing nicking to the trs.

134 rence patterns on the two strands within the RBE and the relative contributions of the individual bas

135 Substitutions within the RBE' also reduce Rep specific activity, but to a lesser

136 focus on the roles of these proteins and the RBEs in controlling transcription during a productive in

137 within this region that did not exhibit this RBE epitope were also non-fusogenic despite their abilit

138 However, both the TR RBE and the p5 RBE transactivate the p19 and p40 promote

139 le tip of an internal hairpin within the TR (RBE'), and the trs.

140 amers that were different from the wild-type RBE in terms of both primary sequence and secondary stru

141 level by 35%, while the expression of the U6 RBE(apt) did not affect p24 production.

142 In contrast to the NMR model of the unbound RBE, an asymmetric G-G pair with N2-N7 and N1-O6 hydroge