ライフサイエンスコーパス: REDOR

1 REDOR indicated that phosphorus was still distant, but n

2 REDOR NMR and synthetic studies established the T-taxol

3 REDOR NMR studies of a 151 kDa complex of uniformly 15N-

4 REDOR revealed that the phosphorus of PEP was cleaved.

5 ge deformations upon ligand binding, 31P-19F REDOR measurements can also serve as an assay for comple

6 We have performed solid-state 31P-19F REDOR nuclear magnetic resonance (NMR) experiments to mo

7 These 31P-19F REDOR results demonstrate the ability of solid-state NMR

8 we present experimental results from 2H{19F} REDOR NMR that provide direct confirmation that paclitax

9 The 15N{31P} REDOR NMR spectra of three metabolically stable phosphon

10 sult suggests that the K3 dimers detected by REDOR at L/P = 20 are not on the surface of the bilayer

11 luorine and carbon nuclei were determined by REDOR.

12 ical distance has been precisely measured by REDOR solid-state NMR spectroscopy in the transmembrane

13 Interhelical (15)N...(13)C REDOR measurements between Gln664 side chains on opposin

14 enrichment, and measured it with (19)F-(13)C REDOR.

15 (15)N{(13)C} REDOR measurements are consistent with a single (13)C-(1

16 d heteronuclear spin pair under conventional REDOR.

17 NMR, CP rotational-echo double resonance (CP-REDOR) NMR, and heteronuclear correlation (HETCOR) NMR s

18 from a new solid-state NMR experiment, DANTE-REDOR, which can determine global secondary structure in

19 simulations of binding restrained by double REDOR-determined intramolecular C-F distances.

20 ble rotational-echo double resonance (double REDOR) has been used to investigate the bound conformati

21 ble rotational-echo double resonance (double REDOR) NMR was used to investigate the conformation of a

22 ar dynamics simulations restrained by double-REDOR-determined intramolecular (13)C-(19)F distances re

23 g a new solid-state NMR technique called DSQ-REDOR, are consistent with hydrogen bonds between side c

24 Full-echo REDOR spectra of cell-wall complexes from cells labeled

25 tance measurement approach, using (13)C(19)F REDOR, to measure a ligand-induced change of 1.0 +/- 0.3

26 sive peptide-headgroup contact, (13)C[(19)F] REDOR experiments on MLVs containing specifically (19)F-

27 Complementary (31)P[(19)F] REDOR experiments on these MLVs show an enhanced headgro

28 lely on results for epimer B, a (15)N[(19)F] REDOR NMR study was performed on the complex formed from

29 olar couplings were measured by (13)C[(19)F] REDOR.

30 obtained from (13)C{(19)F} and (15)N{(19)F} REDOR dephasing allow a correlation of structure and ant

31 The (13)C{(19)F} REDOR dephasing maximum indicates that the secondary bin

32 (15)N{(19)F} REDOR dephasing shows the proximity of the fluorine to t

33 The (13)C{(19)F} and (15)N{(19)F} REDOR spectra show that, in situ, DFPBV binds to the pep

34 yostatin analogues strategically labeled for REDOR NMR analysis.

35 ogues were synthesized, and, as required for REDOR analysis, all proved highly potent with PKC affini

36 From REDOR dipolar recoupling data, the structure and orienta

37 X-Pro peptide bonds in bR are assigned from REDOR difference spectra of pairwise labeled samples, an

38 he distance and orientation information from REDOR is consistent with a parallel (N-N) dimer structur

39 However, REDOR NMR requires strategically labeled, high affinity

40 ed question mark(19)F inverted question mark REDOR with natural-abundance background interferences re

41 ed question mark(15)N inverted question mark REDOR.

42 (17)O-(15)N REDOR curves are presented for glycine.(2)HCl; fits of t

43 ll-echo spectra were removed by (13)C[(15)N] REDOR.

44 In contrast, both (13)C{(15)N} REDOR and (13)C CPMAS are consistent with multiple (13)C

45 The (13)C{(15)N} REDOR experiments resonant at 165 ppm show an incomplete

46 ouble-resonance (REDOR) NMR and (13)C{(15)N} REDOR to determine the chemical identity of these produc

47 In the present study, solid-state NMR REDOR methods were applied for detection of oligomeric b

48 Comparisons of simulated and observed REDOR dephasing were used to deduce a closest approach d

49 f the His37 side chain based on the observed REDOR distance.

50 A combination of REDOR, rotational-echo double-resonance, and conventiona

51 Comparison of REDOR spectra on samples that were labeled at different

52 Based on REDOR results, we determined that cheatgrass (a plant th

53 This model is also consistent with six other REDOR-determined internuclear distances, most of which a

54 Our REDOR experiments indicate that alpha-helical Ala19-maga

55 ues (Ala(46) and Ala(49)) using (13)C{(31)P} REDOR and one lysine residue (Lys(52)) using (15)N{(31)P

56 lysine residue (Lys(52)) using (15)N{(31)P} REDOR.

57 We performed REDOR solid-state NMR experiments on lyophilized samples

58 measurements suggesting that the present PTX REDOR distances may not provide a precise model for bioa

59 restingly, Rotational Echo DOuble Resonance (REDOR) difference spectroscopy of [20-13C]retinal,[indol

60 1)B{(31)P} rotational echo double resonance (REDOR) experiments show systematic deviations from calcu

61 contrast, rotational echo double resonance (REDOR) NMR experiments revealed that the sequence Ala24-

62 using the rotational echo double resonance (REDOR) NMR method.

63 Rotational-echo double resonance (REDOR) NMR provided internuclear distances from the 19F

64 Rotational echo double resonance (REDOR) NMR spectroscopy could uniquely address this chal

65 17O-1H rotational echo double resonance (REDOR) NMR was applied to probe the O-H distances in zeo

66 DR-CT) and rotational echo double resonance (REDOR) solid-state NMR techniques, demonstrate that octa

67 DRAMA) and rotational-echo double resonance (REDOR) to determine intra- and interligand internuclear

68 technique, rotational-echo double resonance (REDOR), can be used to measure both intra- and intermole

69 3)C{(15)N} Rotational Echo DOuble Resonance (REDOR), the structure of the C-terminus was found to be

70 Rotational-echo double-resonance (REDOR) (13)C[(31)P] and (15)N[(31)P] experiments on the

71 Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been ob

72 3)C{(19)F} rotational-echo double-resonance (REDOR) dephasing for the cell-wall (13)C-labeled bridgin

73 sured in a rotational-echo double-resonance (REDOR) experiment performed on mixtures of differently l

74 Rotational-echo double-resonance (REDOR) experiments on [15N]Ser bound to a [1-13C]Phe-rec

75 3)C{(19)F} rotational-echo double-resonance (REDOR) experiments on whole cells enriched with l-[1-(13

76 13)C/(15)N rotational echo double-resonance (REDOR) measurements indicate an antiparallel organizatio

77 5)N{(13)C} rotational-echo double-resonance (REDOR) NMR and (13)C{(15)N} REDOR to determine the chemi

78 S-echo and rotational-echo double-resonance (REDOR) NMR experiments, employing a slow catalytic EPSP

79 1)P[(19)F] rotational-echo double-resonance (REDOR) NMR measurements, we establish that UDG partially

80 onance and rotational echo double-resonance (REDOR) NMR methods.

81 olid state rotational-echo double-resonance (REDOR) NMR was used to probe the internuclear distance b

82 ermined by rotational-echo double-resonance (REDOR) NMR.

83 The rotational-echo double-resonance (REDOR) pulse sequence was used to measure the internucle

84 -selective rotational-echo double-resonance (REDOR) solid-state NMR experiment to measure the concent

85 thors used rotational-echo double-resonance (REDOR) solid-state NMR to measure intermolecular and int

86 using the rotational-echo double-resonance (REDOR) technique under magic-angle spinning.

87 experiment, termed FSR (frequency selective REDOR), combines the REDOR pulse sequence with a frequen

88 sed on the interpretation of two solid-state REDOR (13)C-(19)F distances in a fluorinated PTX derivat

89 The REDOR results indicate a single fluorine dephasing cente

90 The REDOR spectra show that aromatic carbons from the polyst

91 The REDOR technique allows (19)F...(31)P distances to be mea

92 SR (frequency selective REDOR), combines the REDOR pulse sequence with a frequency selective spin-ech

93 ng restrained by distances inferred from the REDOR spectra suggests that all of the 6-fluorotryptopha

94 no significant dephasing was observed in the REDOR experiment in the dark or upon light activation.

95 In the REDOR measurements, observation of reduced (13)C intensi

96 at 165 ppm show an incomplete buildup of the REDOR data to approximately 90% of the expected maximum.

97 d torsions and a justifiable increase of the REDOR distance error to > or = +/-0.7 A readily resolves

98 This study demonstrates the utility of the REDOR NMR technique for the elucidation of the oligomeri

99 (13)C-(19)F separations compatible with the REDOR measurements suggesting that the present PTX REDOR

100 etastable state that are consistent with the REDOR NMR measurements.

101 odel of the binding site consistent with the REDOR results positions the vancomycin cleft around an u

102 ar dynamics simulations constrained by three REDOR-determined distances to His156.

103 We also use REDOR to measure dipolar interactions in [20-13C]retinal

104 roposed covalent intermediates, we have used REDOR to measure the same distances in enzyme-free and e

105 determine the single 31P-31P distance, while REDOR was used to determine one 31P-15N distance and fiv

106 and (31)P spins in the crystal surface with REDOR NMR show that, in the peptide fragment derived fro