ライフサイエンスコーパス: RNA cap

1 TbCMT1 did not affect parasite growth or SL RNA capping.

2 at are essential to virus replication and to RNA capping.

3 gation but also in stimulating nascent viral RNA capping.

4 is is the first observation of ATP-dependent RNA capping.

5 the 5' RNA cap: G0pppAG-RNA --> (m7)G0pppAG-RNA ("cap-0")-->(m7)G0pppAm2'-O-G-RNA ("cap-1").

6 m7)G0pppAG-RNA ("cap-0")-->(m7)G0pppAm2'-O-G-RNA ("cap-1").

7 D6; mRNA capping enzyme subunits D1 and D12; RNA cap 2'-O-methyltransferase; A18 DNA helicase; DNA-de

8 quent modifications characteristic of the SL RNA cap 4 were added successively in a 5' to 3' directio

9 oded methyltransferases (MTases) involved in RNA capping, a guanine-N7-MTase and a ribose-2'-O-MTase.

10 e in complex with guanosine triphosphate and RNA cap analog.

11 establish NAD(+) as an alternative mammalian RNA cap and DXO as a deNADding enzyme modulating cellula

12 ein 5 (NS5) contains a methyltransferase for RNA capping and a polymerase for viral RNA synthesis.

13 replication protein 1a, which has N-terminal RNA capping and C-terminal helicase domains.

14 al surfaces of two of its proteins; that the RNA capping and export apparatus is a hollow cylinder, w

15 ation factors 1a, with domains implicated in RNA capping and helicase functions, and 2a, with a centr

16 RNA replication proteins: 1a, which contains RNA capping and helicase-like domains, and 2a, which is

17 RNA replication proteins: 1a, which contains RNA capping and helicase-like domains, and the polymeras

18 1a, which encodes RNA capping and helicase-like domains, localizes to endo

19 esults extend our understanding of nidovirus RNA capping and methylation beyond coronaviruses and als

20 will end with a summary of novel findings in RNA capping and the questions these findings pose.

21 and an N-terminal domain implicated in viral RNA capping, and 2a contains a central polymerase-like d

22 icase-like domain and a domain implicated in RNA capping, and 2a, which contains a polymerase-like do

23 Here, using an improved oligo-RNA capping assay with the VSV L protein, we showed that

24 Like the RNA-binding activity, RNA capping assays performed with open cores indicates t

25 our understanding of the mechanism of viral RNA cap binding we performed a detailed structural and b

26 dc42 effector, pp70 S6 kinase, stimulate the RNA cap-binding activity of the CBC.

27 Here we identify a component of the nuclear RNA cap-binding complex (CBC), Ars2, that is important f

28 effects on pre-mRNA splicing is the nuclear RNA cap-binding complex (CBC), which plays a key role in

29 tion is followed by formation of a messenger RNA cap-binding complex that includes the initiation fac

30 protein 2 (ARS2), a component of the nuclear RNA CAP-binding complex that is crucial for biogenesis o

31 Our data support the idea that coronavirus RNA capping could be targeted for development of antivir

32 h mTOR is known as a controller of messenger RNA cap-dependent translation initiation, new advances i

33 a multifunctional protein with an N-terminal RNA capping domain and a C-terminal helicase-like domain

34 on, RNA-dependent RNA polymerase (RdRp), and RNA capping domains.

35 The 1a protein has putative helicase and RNA-capping domains, whereas 2a contains a polymerase-li

36 RNA capping enzyme (CE) is recruited specifically to RNA

37 t of the RNA polymerase and also encodes the RNA capping enzyme guanylyltransferase.

38 -molecule inhibitors of the dengue virus NS5 RNA capping enzyme.

39 anylyltransferase activity of the flavivirus RNA capping enzyme.

40 We have examined the RNA-capping enzyme activities of bluetongue virus (BTV)

41 Recent structural studies have shown that RNA capping enzymes and DNA ligases have similar protein

42 (+)-dependent DNA ligases, and GTP-dependent RNA capping enzymes are members of a covalent nucleotidy

43 t contemporary DNA ligases, RNA ligases, and RNA capping enzymes evolved by the fusion of ancillary e

44 Novel applications of RNA capping enzymes in the discovery of new RNA species

45 at comprise the active sites of DNA ligases, RNA capping enzymes, and T4 RNA ligase 2.

46 at comprise the active sites of DNA ligases, RNA capping enzymes, and T4 RNA ligases 1 and 2.

47 f a shared structural basis for catalysis by RNA capping enzymes, DNA ligases, and RNA ligases, which

48 superfamily of RNA ligases, DNA ligases, and RNA capping enzymes.

49 anism and origin of RNA and DNA ligases, and RNA capping enzymes.

50 and V that are conserved in DNA ligases and RNA capping enzymes.

51 ped' by the addition of GMP to the 5" end by RNA capping enzymes.

52 polynucleotide ligases and the GTP-dependent RNA capping enzymes.

53 polynucleotide ligases and the GTP-dependent RNA capping enzymes.

54 xtend our current understanding of nidovirus RNA cap formation and methylation beyond the coronavirus

55 e, and the recently recognized Mesoniviridae RNA cap formation and methylation have been best studied

56 ping enzyme guanylyltransferase activity and RNA cap formation by transcription factor IIH-mediated C

57 sferase activity of human capping enzyme and RNA cap formation.

58 protein catalyzes two reactions involved in RNA cap formation.

59 ect evidence for the importance of the viral RNA capping function.

60 protein contains a helicase-like domain and RNA capping functions.

61 pAG-RNA), leading to the formation of the 5' RNA cap: G0pppAG-RNA --> (m7)G0pppAG-RNA ("cap-0")-->(m7

62 lates the N7 and 2'-O positions of the viral RNA cap (GpppA-RNA --> m(7)GpppA-RNA --> m(7)GpppAm-RNA)

63 7 and ribose 2'-OH methylations of the viral RNA cap (GpppA-RNA-->m(7)GpppAm-RNA).

64 ally catalyzes two methylations of the viral RNA cap, GpppA-RNA-->m(7)GpppA-RNA-->m(7)GpppAm-RNA, by

65 de for nsp14, a bifunctional enzyme carrying RNA cap guanine N7-methyltransferase (MTase) and 3'-5' e

66 RNA cap guanine-N2 methyltransferases such as Schizosacc

67 The smaller size of RNA capping guanylyltransferases from other organisms su

68 ifampicin-binding pocket, suggesting altered RNA capping in Rifampicin-resistant strains.

69 nd Caenorhabditis elegans we have found that RNA capping is also essential for metazoan viability.

70 the mRNA capping apparatus of VSV evolved an RNA capping machinery that functions in a sequence-speci

71 GFP) showed that sequences in the N-terminal RNA capping module of 1a mediate membrane association.

72 typically contains the adenosine 2'OH of the RNA-cap moiety.

73 ose a wide repertoire of potential bacterial RNA capping molecules, and provide mechanistic insights

74 nal enzyme bearing 3'-5' exoribonuclease and RNA cap N7-guanine methyltransferase activities involved

75 ties involved in replication fidelity and 5'-RNA capping, respectively.

76 By establishing a small RNA Cap-seq method that employs the cap-binding protein

77 tivity which catalyses the first step in the RNA-capping sequence.

78 ty and amino acid requirements typical of an RNA cap-specific, m(7)G-dependent N2 methyltransferase.

79 These results define an additional 5' RNA cap structure in eukaryotes and raise the possibilit

80 The SL RNA cap structure in Trypanosoma brucei is unique among

81 The only known RNA cap structure in unicellular protists is the unusual

82 e, which are involved in the modification of RNA cap structure.

83 yme is responsible for methylating the viral RNA cap structure.

84 el of how the flavivirus MTase protein binds RNA cap structures is presented.

85 s to both N7 and 2'-O positions of the viral RNA cap, the GTP-binding pocket functions only during th

86 Tgs1 is the enzyme that converts m(7)G RNA caps to the 2,2,7-trimethylguanosine (TMG) caps char

87 responsible for converting 7-methylguanosine RNA caps to the 2,2,7-trimethylguanosine cap structures

88 s initially identified as being required for RNA cap trimethylation in vivo in budding yeast.

89 ific binding of the methyltransferase to the RNA cap was demonstrated by UV cross-linking to [32P]GMP

90 The RNA capped with the m(7(LNA))G[5']ppp[5']G 3 cap analogu