ライフサイエンスコーパス: RNA cleavage

1 nucleobase general acid strategies to effect RNA cleavage.

2 plays an essential role in the catalysis of RNA cleavage.

3 ell-shaped pH versus k(cat)/K(M) profile for RNA cleavage.

4 ral elements within BLM that control DNA and RNA cleavage.

5 sses such as transcription, translation, and RNA cleavage.

6 loop (TL), for both nucleotide addition and RNA cleavage.

7 thermophilus, inhibits rather than activates RNA cleavage.

8 RISC assembly, or multiple rounds of target-RNA cleavage.

9 lex (RISC), which catalyzes target messenger RNA cleavage.

10 also associate with Ago2 for guiding target RNA cleavage.

11 kely catalyze the proton-relay mechanism for RNA cleavage.

12 ch catalyzes site specific, Pb(2+)-dependent RNA cleavage.

13 by a translational mechanism rather than by RNA cleavage.

14 e protein-siRNA complex that mediates target RNA cleavage.

15 entary sequences without inducing detectable RNA cleavage.

16 egies to achieve overall rate enhancement of RNA cleavage.

17 ysis of the wild-type ribozyme to facilitate RNA cleavage.

18 their ability to enhance enzyme catalysis of RNA cleavage.

19 oximity without involving Argonaute-mediated RNA cleavage.

20 hat are necessary for efficient catalysis of RNA cleavage.

21 which serves as an acid during catalysis of RNA cleavage.

22 mains generating a potential active site for RNA cleavage.

23 nation and, in some cases, cotranscriptional RNA cleavage.

24 tide 320) and that is essential for accurate RNA cleavage.

25 role, to act as an acid during catalysis of RNA cleavage.

26 termediate in factor-induced endonucleolytic RNA cleavage.

27 RNA; however, HDAgs are not required for HDV RNA cleavage.

28 y of HDAg is required for the enhancement of RNA cleavage.

29 -through and carry out SII-activated nascent RNA cleavage.

30 the cleaving site was required for efficient RNA cleavage.

31 mplicated in both sRNA maturation and target RNA cleavage.

32 ich enables rapid recovery from any depth by RNA cleavage.

33 kinetic competition between 1D diffusion and RNA cleavage.

34 Very few microRNAs are known to guide RNA cleavage.

35 ed RNAs is probably created by site-specific RNA cleavage.

36 eting, in vitro data have shown crRNA-guided RNA cleavage.

37 allowing piRNAs to spread beyond the site of RNA cleavage.

38 ributes to human diseases via stress-induced RNA cleavage.

39 rted to be the most efficient lanthanide for RNA cleavage.

40 cellular RISC machinery for inducing target RNA cleavage.

41 ganic phosphate as a nucleophile to catalyze RNA cleavage.

42 ecreted and endocytosed, but fails to induce RNA cleavage.

43 tic tetrad, thereby activating Argonaute for RNA cleavage.

44 ven mRNAs that are resistant to nsp1-induced RNA cleavage.

45 nificantly inhibited human RNase H-catalyzed RNA cleavage (80-90% inhibition) and that a similar inhi

46 entical to that emptied during SII-activated RNA cleavage, a process required for the resumption of e

47 and the distance between the pocket and the RNA cleavage active site in the RNaseIII domain correspo

48 ize the mechanisms of the type I and type II RNA cleavage activities harbored by the Flp (pronounced

49 cific DNA recombinase Flp shows two types of RNA cleavage activities on hybrid DNA-RNA substrates.

50 trand breakage and joining, and two types of RNA cleavage activities.

51 utation of lysine to glutamic acid abolished RNA cleavage activity in the absence of a divalent metal

52 In addition, Fap7 inhibits the RNA cleavage activity of Nob1, the endonuclease responsi

53 levels and activity correlated well with the RNA cleavage activity of the DNAzyme.

54 We present the evidence for the specific RNA cleavage activity of the engineered catalytic subuni

55 tively catalytic residues, has no detectable RNA cleavage activity on its own but is active upon mixi

56 t neither residue contributes to the type II RNA cleavage activity or to the strand-joining reaction

57 ently linked catalytic homodimer that had no RNA cleavage activity upon mixing with the structural su

58 Ribozyme-mediated RNA cleavage activity was detected in cell extracts.

59 ole in splicing involves RNA binding but not RNA cleavage activity.

60 Argonaute2, being responsible for messenger RNA cleavage activity.

61 asmids and DNA viruses requires DNA, but not RNA, cleavage activity.

62 to achieve both enhanced DNA and diminished RNA-cleavage activity.

63 ely 100-fold increase in the ratio of DNA to RNA-cleavage activity.

64 each its catalytic potential as a biomimetic RNA cleavage agent.

65 ating enhanced biomimetic, sequence-specific RNA cleavage agents.

66 The results of RNA cleavage analyses suggest that KHSO5- or magnesium m

67 ns C-terminal RNase III domains that mediate RNA cleavage and an N-terminal helicase motif, whose fun

68 rn2 attacks the 5'PO4-end exposed by nascent RNA cleavage and chases down the RNA polymerase.

69 mes have the potential to perform successive RNA cleavage and joining reactions, resulting in their m

70 The catalysis of site-specific RNA cleavage and ligation by the hairpin ribozyme requir

71 ellite (VS) ribozyme catalyzes site-specific RNA cleavage and ligation reactions.

72 function of CPSF in mediating PAS-dependent RNA cleavage and polyadenylation.

73 e, which shows the conformation required for RNA cleavage and proximity of the 2'-hydroxyl group to t

74 lt of loss of the 3'-UTR due to ASO-mediated RNA cleavage and retention of the last intron.

75 ns on the surface of ER membranes to promote RNA cleavage and ribonucleoprotein (RNP) removal.

76 rent segments of the Rne protein to catalyze RNA cleavage and to bind RNA, we found that the N-termin

77 When TFIIS is present, RNA cleavage and transcriptional restart pathways are su

78 They can catalyze site-specific RNA cleavage, and as a result, they have relevance in ge

79 d Cmr5), each Cmr4 subunit mediates a target RNA cleavage, and Cmr1 and Cmr6 mediate an essential int

80 ly 28 s(-1)) was comparable with that of net RNA cleavage ( approximately 27 nucleotides(s)).

81 t the functions of the TL and Gre factors in RNA cleavage are conserved in various species, with impo

82 demonstrate that miRNA processing and target-RNA cleavage are coupled.

83 At the pause sites, the burst amplitudes of RNA cleavage are larger than the corresponding reaction

84 consistent with a two-metal ion mechanism of RNA cleavage as previously suggested for a number of pol

85 CV John Cunningham 1/AAG mutant and in vitro RNA cleavage assay demonstrated that MCPIP1 could direct

86 In vitro RNA cleavage assay demonstrated that the ribozyme select

87 Here, we used an RNA cleavage assay to show that the PEG and Ficoll crowd

88 RNA cleavage assays showed that S. thermophile Dicer-1 (

89 a- and XBP1-depleted cells, validation using RNA cleavage assays, and 5' RACE identified the prooncog

90 appeared functionally equivalent in in vitro RNA cleavage assays.

91 enzymatic activity of DICER1 using in vitro RNA cleavage assays.

92 ch activity was detected and increased total RNA cleavage at high Mg(2+) concentrations sufficient to

93 ace of Fe(2+) in supporting the catalysis of RNA cleavage at neutral pH, but not at lower pH.

94 for efficient termination, cotranscriptional RNA cleavage at the poly(A) site is not.

95 I, and that loss of EF-RNA interactions upon RNA cleavage at the polyadenylation site triggers disass

96 P23 are essential for early pre-ribosomal (r)RNA cleavages at sites A0, A1/1 and A2/2a in yeast and h

97 Plant microRNAs (miRNAs) typically mediate RNA cleavage, but examples of miRNA-mediated translation

98 ORF17 protein induced RNA cleavage, but to a substantially lesser extent than

99 ozymes have been identified for catalysis of RNA cleavage by 2'-hydroxyl transesterification, forming

100 tify the smallest RNA that can direct target RNA cleavage by 3' tRNase.

101 small interfering RNAs that guide transgene RNA cleavage by AGO1.

102 eletions of the TL strongly impair intrinsic RNA cleavage by all three RNAPs and eliminate the inters

103 a protein structural change that accelerates RNA cleavage by another subunit.

104 Here, we report that RNA cleavage by Argonaute3 initiates production of most

105 se function of HIV-1 RT and then to abrogate RNA cleavage by HIV-1 RNaseH.

106 investigated the ability of DNA to catalyze RNA cleavage by hydrolysis rather than transesterificati

107 We demonstrate that NusG slows RNA cleavage by inhibiting backtracking.

108 The value of k(cat)/K(M) for catalysis of RNA cleavage by ribonuclease (RNase) A can exceed 10(9)

109 s12 and His119 are critical for catalysis of RNA cleavage by ribonuclease A (RNase A).

110 molecules: the light-regulation of catalytic RNA cleavage by RISC and the light-regulation of seed re

111 RNA cleavage by RNA polymerase (RNAP) is the central ste

112 a universal bacterial factor that stimulates RNA cleavage by RNA polymerase (RNAP), the functions of

113 This incongruity indicates that catalysis of RNA cleavage by RNase A is limited by the rate of substr

114 RNA cleavage by RNase H requires the presence of divalen

115 eopure ASOs, 3'-SpSpRp, that promotes target RNA cleavage by RNase H1 in vitro and provides a more du

116 slation initiation factor eIF2 and stimulate RNA cleavage by RNase L.

117 sm for Pol I termination: co-transcriptional RNA cleavage by Rnt1 provides an entry site for the 5'-3

118 RNA cleavage by some endoribonucleases and self-cleaving

119 group I and group II intron RNAs, as well as RNA cleavage by the aI5gamma-derived D135 ribozyme.

120 y and the molecular mechanism that underlies RNA cleavage by the RNase E/G family.

121 of thymidine 5'-p-nitrophenyl phosphate and RNA cleavage by the RNase P ribozyme.

122 GreA factors activate RNA cleavage by wild-type RNAPs to similar levels.

123 MS) to monitor the kinetics and products of RNA cleavage, by use of a program designed to mass-match

124 RNA cleavage can also be stimulated by universal Gre fac

125 enomena of termination and cotranscriptional RNA cleavage can be uncoupled, and the efficiency of bot

126 ts (5' fragments) produced by miRNA-mediated RNA cleavage can be uridylated in plants and animals.

127 in the reactions of nucleotide addition and RNA cleavage catalyzed by RNAP.

128 /mol reduction in activation free energy for RNA cleavage catalyzed by the HDV ribozyme.

129 Reciprocal cycles of piRNA-directed RNA cleavage--catalyzed by the PIWI proteins Aubergine (

130 Despite the conserved RNA cleavage chemistry and a similar enzyme assembly, cu

131 Measurement of the steady state rates of RNA cleavage confirms that all substrates dissociate slo

132 ide was released from the complex by nascent RNA cleavage, demonstrating that this interaction takes

133 We combine a general RNA cleavage domain with a series of Pumilio/fem-3-bindi

134 The RNA cleavage efficiency was found in all cases to be spe

135 in vivo reveals a second co-transcriptional RNA cleavage event at T1 which provides Pol I with an al

136 RNA-mediated RNA cleavage events are being increasingly exploited to

137 Thus, RNA cleavage events catalyzed by RNase L are required fo

138 doplasmic reticulum unfolded protein load to RNA cleavage events that culminate in the sequence-speci

139 les of the poly(A) signal, cotranscriptional RNA cleavage events, and 5'-3' exonucleolytic RNA degrad

140 subunit Rpb9p, and the pol II elongation and RNA cleavage factor, TFIIS, respectively.

141 pproach to study the requirements of hairpin RNA cleavage for sugar and base moieties in residues of

142 in Saccharomyces cerevisiae does not rely on RNA cleavage for termination but instead terminates via

143 aG), which serves as the attacking group for RNA cleavage, forms a coplanar base triple with the G264

144 oth the full-length, and all of the possible RNA cleavage fragments that resulted from the combinatio

145 We used an nsp1 mutant, nsp1-CD, lacking the RNA cleavage function, to delineate the mechanism of nsp

146 t the role of metal ions in the mechanism of RNA cleavage has not been resolved.

147 nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency al

148 templates that are resistant to nsp1-induced RNA cleavage, implying the validity of using nsp1-CD to

149 aPKR, for their ability to effect target PKR RNA cleavage in a cell-free and in an intact cell assay,

150 de a cynosure for understanding catalysis of RNA cleavage in a system of high medicinal relevance.

151 o significantly affect the rate of intrinsic RNA cleavage in a TL-dependent manner.

152 experimental measurement on the spontaneous RNA cleavage in an in vitro evolved ATP aptamer motives

153 promoter complexes whereas GreB facilitates RNA cleavage in arrested elongation complexes (ECs).

154 e angiogenin acts unidirectionally to induce RNA cleavage in astroglia, while the ALS-associated K40I

155 Rho- or Mfd-mediated RNA release or nascent RNA cleavage in backtracked complexes, the regulatory ta

156 her the TL nor GreA can efficiently activate RNA cleavage in certain types of backtracked transcripti

157 s like RNase H and is responsible for target RNA cleavage in RNA interference.

158 d deglycoBLM analogues were shown to mediate RNA cleavage in the absence of added Fe(2+).

159 In vitro, NoV B2 inhibits Dicer-mediated RNA cleavage in the absence of any other host factors an

160 essures, such as the requisite of catalyzing RNA cleavage in the absence of Mg(2+) or Mn(2+).

161 ex DNA, sequence-selective DNA cleavage, and RNA cleavage in the presence and absence of a metal ion

162 self-cleaving RNA that can be engineered for RNA cleavage in trans and has potential as a therapeutic

163 The requirement for two modes of RNA cleavage in viral replication and the unexpected req

164 ed AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required fo

165 mechanism for the differential regulation of RNA cleavages in E. coli.

166 at the RNA binding property of VapC-mt4, not RNA cleavage, initiates toxicity.

167 d a 6-base recognition sequence, UACAUA, for RNA cleavage instead of the 5-base sequence, UACAU, for

168 We also demonstrate that CRISPR-based RNA cleavage is effective for regulation in bacteria, ar

169 Substrate RNA cleavage is gRNA directed and occurs 3' to the uridy

170 It is not clear whether RNA cleavage is sufficient to trigger nuclear RNA degrad

171 tion as a possibly important intermediate in RNA cleavage, its structure has been captured in various

172 riety of chemical transformations, including RNA cleavage, ligation, and synthesis, as well as alkyla

173 9, RPP30 and L7Ae-EDTA-Fe) revealed specific RNA cleavages, localizing the binding sites of L7Ae to t

174 ity to down-regulate gene expression through RNA cleavage makes the hammerhead ribozyme a candidate f

175 We suggest that SymE promotion of RNA cleavage may be important for the recycling of RNAs

176 w that a lincRNA-specific co-transcriptional RNA cleavage mechanism acts to induce premature terminat

177 We describe a new RNA cleavage motif, found in the hammerhead ribozyme.

178 cleotide incorporation, sequential rounds of RNA cleavage occurred each time after approximately 6 nu

179 In accord with earlier studies with model RNAs, cleavage occurs only in the presence of manganese

180 Furthermore, our analysis showed that the RNA cleavage pathway is also present in human cells but

181 y cap binding to the PB2 subunit, from which RNA cleavage preferentially occurs at the 12th nt downst

182 rived from the HIV-1 genome, six predominant RNA cleavage products are found during DNA synthesis cat

183 rast, both intrinsic and TFIIS-induced small RNA cleavage products are very similar when produced fro

184 line, Hey1b, resulted in specific ribosomal RNA cleavage products coinciding with JNK activation.

185 The RNA cleavage products consisted of a 3' phosphate and 5'

186 -linked oligoadenylate (2-5A) produces small RNA cleavage products from self-RNA that initiate IFN pr

187 RNA cleavage products generated by RNase L enhance IL-1b

188 fter WNV infection and the patterns of viral RNA cleavage products generated were similar in both typ

189 Enzymatic analysis of RNA cleavage products has suggested that human immunodef

190 mass-match observed MS peaks with predicted RNA cleavage products.

191 he first of their kind in terms of their DNA-RNA cleavage properties, and they may have important bio

192 However, the major function of His119 in RNA cleavage, protonation of the 5'-O leaving group, is

193 apsid-specific antiserum eliminated specific RNA cleavage provides further evidence that the virus ca

194 6P is used by the ribozyme as a coenzyme for RNA cleavage, rather than an allosteric effector.

195 The RNA cleavage reaction catalyzed by the hairpin ribozyme

196 The hairpin ribozyme catalyzes a reversible RNA cleavage reaction that participates in processing in

197 hepatitis delta virus ribozyme catalyzes an RNA cleavage reaction using a catalytic nucleobase and a

198 site captured the pre-catalytic state of the RNA cleavage reaction, illustrating the unexpected Pb(2+

199 n ribozyme, catalyzes a multistep reversible RNA cleavage reaction, which comprises two structural tr

200 lge backbones that are poised for an in-line RNA cleavage reaction.

201 combination active site, exhibits the type I RNA cleavage reaction.

202 uses histidine as an active component for an RNA cleavage reaction.

203 emplate arrest sites by activating a nascent RNA cleavage reaction.

204 ulate the free energy surface underlying the RNA-cleavage reaction and characterize its mechanism.

205 oordination emerge as central factors in the RNA-cleavage reaction.

206 activity was detected during activation and RNA cleavage reactions with human RNase L.

207 ns as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression.

208 llance and how it carries out crRNA-mediated RNA cleavage remain unclear.

209 e contributions of the TL and Gre factors to RNA cleavage reportedly vary between RNAPs from differen

210 little or no influence over single-stranded RNA cleavage, RI evasion or cytotoxicity.

211 In addition, RNA cleavage site choice by the full polymerase is also

212 However, the two enzymes showed identical RNA cleavage site preferences with an mRNA as substrate.

213 These major RNA cleavage sites correlate well with the pause sites s

214 related to vertebrate and yeast primary pre-RNA cleavage sites, respectively.

215 The RNA cleavage ("Slicer") activity of Argonaute has been i

216 AGO1-catalyzed RNA cleavage (slicing) represses miRNA targets, but curr

217 RNA cleavage specificity for these VapCs mapped to motif

218 der oligomer species that possesses distinct RNA cleavage specificity from that of previously charact

219 We show that this RNA cleavage step is essential for assembly of the Csy p

220 ic activities, including DNA polymerization, RNA cleavage, strand transfer, and strand displacement s

221 of the FL and TL mutations on GreA-assisted RNA cleavage suggest that the FL-dependent TL transition

222 the resulting enzymes are more efficient at RNA cleavage than most Mg(2+)-dependent nucleic acid enz

223 RNase A is a far more efficient catalyst of RNA cleavage than ONC but is not cytotoxic.

224 y-detailed and semi-quantitative analysis of RNA cleavage that should be widely applicable.

225 Analysis of donor RNA cleavage, the acceptor invasion site and R homology

226 hough it is dispensable for 5'-end-dependent RNA cleavage, the carboxy-terminal half of RNase E signi

227 from shallow backtracks by 1D diffusion, use RNA cleavage to recover from intermediary depths, and ar

228 in RNA interference (RNAi) pathways to guide RNA cleavage, translational repression, or methylation o

229 ities of cross-linked ribozymes to carry out RNA cleavage under single turnover conditions were compa

230 e describe the molecular mechanism of target RNA cleavage using affinity-purified minimal RISC from h

231 To confirm that ribozyme-catalyzed RNA cleavage was actually needed for inhibition, we perf

232 The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based

233 RNA cleavage was observed only in the absence of zinc.

234 by its ability to induce an endonucleolytic RNA cleavage, was separable from its translation inhibit

235 hat might be responsible for double-stranded RNA cleavage, we analysed csp41a and csp41b knock-out mu

236 Using nucleotide modifications that inhibit RNA cleavage, we show that R- but not L-sshRNAs require

237 Sites of RNA cleavage were mapped by sequencing reactions.

238 rovements in efficiency at sequence-specific RNA cleavage when compared with analogous o-phenanthroli

239 ontaining complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency o