ライフサイエンスコーパス: RNA transcript

1 II), synthesizing a complementary messenger RNA transcript.

2 gested by a 10.4-fold reduction in L-protein RNA transcript.

3 f the beta subunit, blocking the path of the RNA transcript.

4 ds to the 3'-untranslated region of the pgdA RNA transcript.

5 change on specific nucleotide sequence in an RNA transcript.

6 predicted to alter the splicing of the DOK7 RNA transcript.

7 gene within sequences of BGL3, an intergenic RNA transcript.

8 aplotype and located in the 5' region of the RNA transcript.

9 t induces alternative splicing of a cellular RNA transcript.

10 imately 45 degrees upon synthesis of an 8-nt RNA transcript.

11 on when a DNA duplex is invaded by a nascent RNA transcript.

12 coordinate strand annealing and the nascent RNA transcript.

13 the highly controlled release of the nascent RNA transcript.

14 lls, SUV39H1 associates with alpha-satellite RNA transcripts.

15 hput sequencing of DNA and its corresponding RNA transcripts.

16 events, gene fusions and other variations in RNA transcripts.

17 o acids increase hepatic cyclin D1 messenger RNA transcripts.

18 esholds in deep-sequencing datasets of short RNA transcripts.

19 le, leading to the release of short abortive RNA transcripts.

20 NA molecules that are complementary to known RNA transcripts.

21 hundreds or even thousands of protein-coding RNA transcripts.

22 tially reduces the levels of Pol V-dependent RNA transcripts.

23 aB signaling, and heat shock protein pathway RNA transcripts.

24 have revealed a large number of un-annotated RNA transcripts.

25 ion of expressed genes and reconstruction of RNA transcripts.

26 hancers, MyoD1-binding events, and noncoding RNA transcripts.

27 using a series of full-length prequantified RNA transcripts.

28 oteins to repress the stability of messenger RNA transcripts.

29 rand-specific representation of nonribosomal RNA transcripts.

30 ial for the accumulation of HDV RNA-directed RNA transcripts.

31 t in trans to regulate the activity of other RNA transcripts.

32 eins in the regulation of a diverse array of RNA transcripts.

33 in punctate nuclear foci and binds AGO4 and RNA transcripts.

34 rs and were profiled for greater than 22,000 RNA transcripts.

35 over a few hours in the form of unprocessed RNA transcripts.

36 n the spliceosome-mediated splicing of viral RNA transcripts.

37 by alternative splicing of the primary HIV-1 RNA transcripts.

38 tified a number of other likely, novel small RNA transcripts.

39 anism's transcriptome, the sum of all of its RNA transcripts.

40 pendent messenger RNA levels and splicing of RNA transcripts.

41 promoter controlling the expression of novel RNA transcripts.

42 agments do not lead to observable changes in RNA transcripts.

43 ions to produce genome- and subgenome-length RNA transcripts.

44 ons as single units from precursor messenger RNA transcripts.

45 identification of protein-coding regions in RNA transcripts.

46 ulation of both spliced and nonspliced viral RNA transcripts.

47 se-pairing of associated siRNAs with nascent RNA transcripts.

48 late for RNA polymerase V-mediated noncoding RNA transcripts.

49 TAP in the nuclear export of gammaretroviral RNA transcripts.

50 a that intronic RNAs can reverse splice into RNA transcripts, a crucial step for an influential model

51 become the assay of choice for interrogating RNA transcript abundance and diversity.

52 Messenger RNA transcript abundance did not reliably predict protei

53 R) to measure relative fecal IL-8 and CXCL-5 RNA transcript abundances, and quantitative PCR to enume

54 These RNA transcripts act as competing endogenous RNAs (ceRNAs

55 s systematic nucleotide insertion throughout RNA transcripts, altering previous views that RNA editin

56 e cyclin T variants, is in the elongation of RNA transcripts, although functions related to the initi

57 Due to the large sizes and abundance of the RNA transcripts, an efficient and accurate RNA structure

58 d allow alteration and imaging of endogenous RNA transcripts analogous to CRISPR/Cas-based genomic to

59 RNA transcript analyses of erythroid cells from controls

60 d from Salmonella 5'-leader of the ribosomal RNA transcript and has a 'stem' structure-containing pre

61 lie within the sequence or structure of the RNA transcript and it has been proposed that G-quadruple

62 reduced to two steps, namely release of the RNA transcript and release of the DNA template.

63 on by promoting both the dissociation of the RNA transcript and the closing of the transcription bubb

64 that persistent hybrids between the nascent RNA transcript and the template DNA strand at CTG.CAG tr

65 in the length and sequence at the 5' end of RNA transcripts and can be important for gene regulation

66 l of HIV, and with levels of HLA-C messenger RNA transcripts and cell-surface expression, but the mec

67 lternative splicing expands the diversity of RNA transcripts and plays an important role in tissue-sp

68 g technologies facilitate discovery of novel RNA transcripts and protein isoforms, applications rangi

69 Interestingly, messenger RNA transcripts and proteins involved in chlorophyll bre

70 ) for the simultaneous detection of specific RNA transcripts and proteins, greatly enhancing the spat

71 he absence of viral early and late messenger RNA transcripts and proteins.

72 lymphocytes triggers the return of XIST/Xist RNA transcripts and some chromatin marks (H3K27me3, ubiq

73 hanced the cytoplasmic accumulation of viral RNA transcripts and the expression of viral proteins wit

74 dertake an unbiased, total genome screen for RNA transcripts and their protein products that affect a

75 the DNA strand of origin for any particular RNA transcript, and (ii) quantify the number of sense an

76 eotide, catalyze its addition to the growing RNA transcript, and move stepwise along the DNA until a

77 -elongation transition, the synthesis of the RNA transcript, and the release of core RNAP and nascent

78 ly 16,000) barcoded sequences, production of RNA transcripts, and analysis of transcript ends and tra

79 smic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of gammaretro

80 sequencing (NGS) methodologies by targeting RNA transcripts, and therefore detecting only replicatin

81 y binding complementary regions in messenger RNA transcripts, and they have been broadly implicated i

82 teractions between RNAP subunits F/E and the RNA transcript are pivotal to the molecular mechanisms o

83 RNA transcripts are bound and regulated by RNA-binding p

84 ctor family 2 (TFF2) protein, TFF2 messenger RNA transcripts are concentrated in cells above the neck

85 Probes for RNA transcripts are designed to bind single transcripts.

86 RNA transcripts are subject to posttranscriptional gene

87 Recent studies have identified RNA transcripts arising from mammalian telomeres with th

88 that the Escherichia coli replisome uses the RNA transcript as a primer to continue leading-strand sy

89 y binding both to loop structures within the RNA transcript as well as to eIF4AI.

90 We have termed such RNA transcripts as competing endogenous RNAs (ceRNAs).

91 with this assay and enables the detection of RNA transcripts at rates comparable to workflows not inc

92 this suggests that the retention of nascent RNA transcripts at their site of expression represents a

93 eins, where sequence-specific binding to two RNA transcripts, ATPH: and PSAJ, HAS BEEN DEMONSTRATED T

94 assessed the effectiveness of a whole-blood RNA transcript-based model as a prognostic biomarker in

95 repressed by a conserved noncoding antisense RNA transcript, BDNF-AS.

96 re not dormant but were generating unspliced RNA transcripts before treatment was interrupted.

97 s and release of short (2 to 15 nucleotides) RNA transcripts-before productive initiation.

98 as a standalone RNase that degrades invader RNA transcripts, but the mechanism linking invader sensi

99 tribution of N(6)-methyladenosine (m(6)A) in RNA transcripts by analyzing different subcellular fract

100 Massive parallel sequencing of RNA transcripts by next-generation technology (RNA-Seq)

101 Direct labeling of nascent RNA transcripts by the incorporation of a second fluoroc

102 by RNA polymerases in vitro or in vivo, the RNA transcript can thread back onto the template DNA str

103 Here, we demonstrate that protein-coding RNA transcripts can crosstalk by competing for common mi

104 e transcription and the technical failure of RNA transcript capture, often render traditional dimensi

105 ced tethering of CG9754 to nascent messenger RNA transcripts causes cotranscriptional silencing of th

106 TRBO-mediated expression of an RNA transcript consisting of an sgRNA adjoining a GFP pr

107 Whereas RNase E only poorly degrades RNA transcripts containing a 5' hydroxyl group, RNase J1

108 n has been facilitated by discovery of short RNA transcripts containing the DIS stem-loop 1 (SL1), wh

109 ccessfully identified thousands of mammalian RNA transcripts containing the modification, it is extre

110 Transcript slippage occurs when an RNA transcript contains a repetitive sequence that allow

111 bind directly to DNA or to a sense-stranded RNA transcript corresponding to the known promoter regio

112 wer of high-throughput sequencing to measure RNA transcript counts at an unprecedented accuracy.

113 Analysis of RNA transcripts detected fusion of the HPV/Myc genes, ar

114 ion of 5F-U or 5F-C analogues into HIV-2 TAR RNA transcripts does not significantly alter the RNA str

115 eq) to simultaneously probe all intermediate RNA transcripts during transcription by stalling elongat

116 t ( approximately 160-nucleotide) non-coding RNA transcript employed by adenoviruses to subvert the i

117 Specifically, abundance of RNA transcripts encoded by the DUX4 locus correlated to

118 of tandem sequences present in the 5' UTR of RNA transcripts encoding several tryptophan metabolism g

119 ends when a stop codon in a gene's messenger RNA transcript enters the ribosomal A site.

120 nd remarkably high levels of retrotransposon RNA transcripts, especially for some human endogenous re

121 Infectious viruses, replicons, and RNA transcripts expressed a p8 minicore containing the C

122 characterization of previously un-annotated RNA transcripts expressed by the spirochete during murin

123 MicroRNAs (miRNAs) are small noncoding RNA transcripts expressed throughout the brain that can

124 analysis was carried out on the IgA and IgG RNA transcripts expressing IGHV3 genes in all parotid bi

125 ric (FDM) identifies regions of differential RNA transcript expression between pairs of splice graphs

126 ity: nucleotide selection and incorporation, RNA transcript extension, and proofreading.

127 Because countless RNA transcripts feature single-stranded-dsRNA junctions

128 actively transcribed, as measured by primary RNA transcript FISH.

129 h, which correlated with an upregulation of RNA transcripts for ecm and a decrease in the levels of

130 s that flank the 16S region of the ribosomal RNA transcript, forming a complex with RNA polymerase th

131 of a virion RNA heterodimer comprised of an RNA transcript from an endogenous polytropic virus and a

132 t from an endogenous polytropic virus and an RNA transcript from an exogenous ecotropic MuLV RNA.

133 alternative splicing, result in two or more RNA transcripts from a DNA template.

134 ocess that leads to the creation of multiple RNA transcripts from a single gene.

135 20 also results in accumulation of noncoding RNA transcripts from centromeric cores, a feature common

136 splicing in Drosophila, we sequenced nascent RNA transcripts from Drosophila S2 cells as well as from

137 y(A) truncation cassette inserted to prevent RNA transcripts from elongation through KvDMR1.

138 was detected in Huh7 cells transfected with RNA transcripts from mutant hHVRd2, as evidenced by expr

139 kens intrahepatically inoculated with capped RNA transcripts from mutants aHVRd1 and aHVRd2 developed

140 oblasts with non-replicating, non-translated RNA transcripts from RNA viral genomes with differing de

141 nucleotide, which causes the majority of the RNA transcripts from SMN2 to lack exon 7.

142 -phage DNA (10(5)-10(7) starting copies) and RNA transcripts from the GAPDH housekeeping gene (5.45 n

143 uced by alternative splicing of long primary RNA transcripts from the Ig heavy chain (Igh) locus and

144 with in vitro-transcribed full-length capped RNA transcripts from the infectious clones of each singl

145 pigs intrahepatically inoculated with capped RNA transcripts from the mutants, whereas mutant sHVRd1

146 RNA transcripts from this cDNA and the viruses encoded b

147 drives the expression of a 7.9-kb noncoding RNA transcript (from the Dynamin-3 gene intron) that enc

148 After 14 days, 16S ribosomal RNA transcripts/genome declined 96%, indicating slow gro

149 Although these novel usHIV-RNA transcripts had exon structures that were different

150 generation sequencing, the ability to purify RNA transcripts has become a critical issue.

151 d, particularly when hybridized to a nascent RNA transcript, has been an enigma.

152 here more than 60% of cDNAs derived from K12 RNA transcripts have G at coordinate 117990, there is no

153 talled-transcription assay, we revealed that RNA transcripts helped to populate quadruplexes at the e

154 r the expression and localization of a model RNA transcript in live Escherichia coli cells.

155 s RNAP-RNA contacts that stabilize the short RNA transcript in the active site and demonstrates that

156 ts with elongating complexes and the nascent RNA transcript in ways that stimulate pausing and termin

157 r transfection agents and recognizing target RNA transcripts in a sequence-specific manner.

158 MBs can be used to image and quantify single RNA transcripts in a wide range of cell lines.

159 Thus, considering the abundance of RNA transcripts in cells, RNA may have a marked impact o

160 action (PCR) was performed to measure HLA-DR RNA transcripts in conjunctival cells taken by impressio

161 PRO-seq) and analyzed nascent mtDNA-encoded RNA transcripts in diverse human cell lines and metazoan

162 dance and localization of nascent and mature RNA transcripts in fixed, single cells.

163 sed a 13-fold increase in HERV-K (HML-2) gag RNA transcripts in Jurkat T cells and a 10-fold increase

164 re we present a strategy to visualize single RNA transcripts in living cells using molecular beacons

165 that RBMBs can also be used to image single RNA transcripts in living cells, when the target RNA is

166 Analysis of 5'-capped RNA transcripts in mutant cell lines identified the usag

167 e-copy DNA targets and corresponding nascent RNA transcripts in preimplantation embryos and during sp

168 and distribution of proteins and individual RNA transcripts in single cells.

169 cond layer of information is embedded in all RNA transcripts in the form of RNA structure.

170 The catalytic role of RNA transcripts in the GQ formation was strongly suggest

171 Most unwanted RNA transcripts in the nucleus of eukaryotic cells, such

172 acilitates the production of Pol V-dependent RNA transcripts in the RdDM pathway.

173 ory response for both genes and unique small RNA transcripts in the segmental dosage series we tested

174 developed based on digital gene counting of RNA transcripts in urine as a means to detect prostate c

175 Modular analyses of RNA transcripts in whole blood previously identified an

176 In budding yeast, a number of these unwanted RNA transcripts, including spliced-out introns, are firs

177 hase of EBV infection, noncoding EBV-encoded RNA transcripts induced cellular cytokine synthesis, and

178 Single-stranded RNA transcripts induced interferon-beta mediated though

179 to convert centromeric pre-small interfering RNA transcripts into small interfering RNAs, are defecti

180 When the G-rich portion of the RNA transcript is downstream from the 5' end of the tran

181 ases antitermination, showing that an intact RNA transcript is required to stabilize the interaction

182 Adenosine-to-inosine (A-to-I) editing of RNA transcripts is an increasingly recognized cellular s

183 Here we show that specific RNA transcript isoforms of alpha-synuclein with an exten

184 Eukaryotic genes generate multiple RNA transcript isoforms though alternative transcription

185 ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of tr

186 pairing to recognize sequences in messenger RNA transcripts, leading to translational repression and

187 50-fold when ADAR1 was coexpressed, whereas RNA transcript levels changed by less than 2-fold.

188 demonstrated different (P < 0.05) messenger RNA transcript levels in 148 genes.

189 tial and altitudinal variations in messenger RNA transcript levels of ReCHSs correlating positively w

190 Early studies looking at RNA transcript levels seemed to suggest overexpression i

191 RNA transcript levels were assessed using whole genome m

192 eQTLs are genetic loci that correlate with RNA transcript levels.

193 muscle transcriptome also revealed 461 novel RNA transcripts, likely muscle-expressed long non-coding

194 40 microRNAs and their host long non-coding RNA transcript (lnc-MGC) are coordinately increased in t

195 we report that CCAT2, a novel long noncoding RNA transcript (lncRNA) encompassing the rs6983267 SNP,

196 iously unannotated intergenic long noncoding RNA transcripts (lncRNA) or isoforms that are associated

197 In this study, we demonstrated that RNA transcripts made in vitro from the full-length genom

198 ing NF-Y, p53, and sp1, indicating that such RNA transcripts may function as decoy targets or traps f

199 osed packets can transfer specific proteins, RNA transcripts, microRNAs, and even DNA to target cells

200 here we present evidence that mitochondrial RNA transcripts (mtRNA) are not limited to policystronic

201 that in Schizosaccharomyces pombe telomerase RNA transcripts must be processed to generate functional

202 which we named myosin heavy-chain-associated RNA transcripts (Myheart, or Mhrt), are cardiac-specific

203 cently uncovered a large number of noncoding RNA transcripts (ncRNAs) in eukaryotic organisms, and th

204 we have identified new sense and anti-sense RNA transcripts, novel mRNAs and mi/siRNA-sized RNA frag

205 se single-genome sequencing to find that the RNA transcripts observed following LRA administration ar

206 third chimpanzee (CH1579) was infected from RNA transcripts of a consensus cDNA of HC-TN (pHC-TN).

207 ith its host, it is necessary to analyze the RNA transcripts of both the host and pathogen throughout

208 psies after chemotherapy displayed increased RNA transcripts of genes associated with CSCs and TGF-be

209 The RNA transcripts of genes controlled by this mechanism co

210 In Gram-positive bacteria, the RNA transcripts of many amino acid biosynthetic and amin

211 RNA transcripts of mutant poliovirus cDNA clones were tr

212 approach to discover new oncogene-regulated RNA transcripts of potential clinical relevance in cance

213 ructure of approximately 640-nucleotide (nt) RNA transcripts of the C-rich and G-rich strands of mamm

214 the amounts of both full-length and internal RNA transcripts of U3-minus vectors are reduced in the n

215 Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in

216 accumulations of both spliced and unspliced RNA transcripts of XMRV and MLV, resulting in their nucl

217 2 of its CTD is detected at the same time as RNA transcripts on nascent DNA.

218 of the 8 internal viral proteins, the viral RNA transcripts, or the host response to these molecules

219 cent work, however, has shown that noncoding RNA transcripts overlap chromosomal DNA.

220 incorporation of synthetic nucleosides into RNA transcripts, particularly at or near the promoter.

221 dence suggests that monitoring the noncoding RNA transcript PCA3 in urine may be useful in detecting

222 e consistent inability of cells to recognize RNA transcripts possessing GORS extended to downstream d

223 This variant alters RNA-transcript processing and results in a 222 bp in-fra

224 g of 'transcriptomes'--the collection of all RNA transcripts produced at a given time--from worms to

225 The toxic RNA transcripts produced from the mutant allele alter th

226 comparative genomic hybridization (CGH) and RNA transcript profiling, and we compared the genomic di

227 equence, including epigenetic modifications, RNA transcripts, proteins, and metabolites, which togeth

228 thin tens of milliseconds in the presence of RNA transcripts provided justification for the co-transc

229 n through direct incorporation into a target RNA transcript rather than through a traditional antisen

230 uential assembly of the human spliceosome on RNA transcripts regulates splicing across the human tran

231 In addition, approximately half of the RNA transcripts remain unspliced and either are used to

232 port of partially spliced or unspliced HIV-1 RNA transcripts requires binding of the viral protein re

233 s) are small sequences of DNA able to target RNA transcripts, resulting in reduced or modified protei

234 ng and increased destruction of nascent TERC RNA transcripts, resulting in telomerase deficiency and

235 studies using high-throughput sequencing of RNA transcripts (RNA-seq) of an isogenic DeltaicgR mutan

236 transcriptome (high-throughput sequencing of RNA transcripts [RNA-seq]) data reveals that few of the

237 This study investigates the ribosomal RNA transcript secondary structure in corals as confirme

238 ribed into RNA, and studies with a synthetic RNA transcript sequence demonstrated formation of a high

239 RNA transcript splicing was highly conserved between RFH

240 odel is the cotranscriptional folding of the RNA transcript, sterically inhibiting the extent of back

241 Non-coding RNA transcripts such as microRNAs (miRNAs) and long non-

242 alyzes both nucleotide addition required for RNA transcript synthesis and excision of incorrect nucle

243 rom Rtt103 reduces binding to Rrp6/Trf4, and RNA transcripts terminated by Nrd1(CID(Rtt103)) are pred

244 For some of the RNA transcripts tested, binding and splicing activity of

245 ntaining either a 7- or an 8-nucleotide (nt) RNA transcript that illuminate intermediate states along

246 These studies introduce a novel T-cassette RNA transcript that improves RNA display from a four-way

247 2a cluster, also known as 'oncomiR-1', is an RNA transcript that plays a pivotal regulatory role in c

248 us RNAs (ceRNAs) were recently introduced as RNA transcripts that affect each other's expression leve

249 uency in RSV-infected cells and yields small RNA transcripts that are heterogeneous in length but mos

250 ing and analyzing diverse classes of primary RNA transcripts that are of increasing biological intere

251 enome mutation, but also in the diversity of RNA transcripts that are packaged.

252 study at once the entire and complete set of RNA transcripts that are produced by the genome.

253 roRNAs (miRNAs) are small non-protein coding RNA transcripts that can regulate the expression of mess

254 While most gene transcription yields RNA transcripts that code for proteins, a sizable propor

255 9, are expressed in Wolbachia from noncoding RNA transcripts that contain precursors with stem-loop s

256 polyadenylation (ApA) to generate messenger RNA transcripts that differ in the length of their 3' un

257 t posttranscriptional modifications of viral RNA transcripts that do not change the nucleotide sequen

258 The identification of the presence of large RNA transcripts that do not code for proteins but that m

259 a sizable proportion of the genome generates RNA transcripts that do not code for proteins, but may h

260 iscovery of extensive transcription of large RNA transcripts that do not code for proteins, termed lo

261 transcriptionally active proteins as well as RNA transcripts that have been subjected to adenosine-to

262 MicroRNAs (miRNAs) are short non-coding RNA transcripts that have the ability to regulate the ex

263 Here, we identify RNA transcripts that overlap the cyclooxygenase-2 (COX-2

264 motifs in the untranslated regions (UTRs) of RNA transcripts that sense metabolite levels and modulat

265 In an RNA transcript, the 2'-OH group at the 3'-terminal nucle

266 is study, we demonstrate successful usage of RNA transcript therapy (RTT) as an exogenous human FXN s

267 Most protein coding genes generate multiple RNA transcripts through alternative splicing, variable 3

268 st-transcriptional level and use an extended RNA transcript to co-localize all circuit sensing, compu

269 The gene that produces the precursor RNA transcript to the three largest structural rRNA mole

270 the ability of relatively unstructured model RNA transcripts to activate PKR to inhibit translation,

271 ents and guide Argonaute proteins to nascent RNA transcripts to induce co-transcriptional gene silenc

272 adaptable and expandable system of in vitro RNA transcripts to serve as a combined positive control

273 on regulators into the nucleus and export of RNA transcripts to the cytoplasm.

274 racterized by the ability of a wide range of RNA transcripts to vie for microRNA binding and alleviat

275 ousands of long intergenic non-coding (linc) RNA transcripts, together with recent evidence that linc

276 s Project was launched to contribute maps of RNA transcripts, transcriptional regulator binding sites

277 d for Ser(5)-phosphorylated Pol II and short-RNA transcripts, two hallmarks of transcription initiati

278 le value for the gene expression level of an RNA transcript using one of a growing number of statisti

279 of the full range of cellular polyadenylated RNA transcripts using a Helicos Genetic Analysis system.

280 iption start sites (TSSs) and validate novel RNA transcripts using Northern blots and luciferase prom

281 ic expression of multiple HTR2A messenger (m)RNA transcript variants.

282 ncode IgM and IgD from heterogeneous nuclear RNA transcripts via alternative splicing, lack intron an

283 TV-transfected cells, a separate subgenomic RNA transcript was not required for the initial transfec

284 By comparing and characterizing these RNA transcripts, we conclude that the mechanism of speci

285 of exonic sequences in the splicing of human RNA transcripts, we conducted saturation mutagenesis of

286 Furthermore, full-length RNA transcripts were also defective in terms of nuclear

287 , tryptase, and carboxypeptidase A messenger RNA transcripts were detected by quantitative reverse tr

288 RNA transcripts were extremely stable, showing minimal d

289 Circular RNA transcripts were first identified in the early 1990s

290 ATIII RNA transcripts were identified within CNS lymphomas, an

291 we report here that some of these C. parvum RNA transcripts were selectively delivered into the nucl

292 ort of unspliced and partially spliced viral RNA transcripts, which encode the viral genome and the g

293 cies such as long noncoding RNA and circular RNA transcripts whose presence had not been previously o

294 emains unresolved because replacement of the RNA transcript with a neocassette has no obvious phenoty

295 mation of fully modified and highly emissive RNA transcripts with (th)G replacing all guanosine resid

296 ts (SNVs) in exons and introns on individual RNA transcripts with high efficiency.

297 detected </=10 copies/reaction of quantified RNA transcripts, with a linear dynamic range of 8 log un

298 is cotranscriptionally recruited to nascent RNA transcripts, with particular enrichment at intronic

299 quires localizing the sequences of expressed RNA transcripts within a cell in situ.

300 be used to accurately quantify the number of RNA transcripts within individual cells.