ライフサイエンスコーパス: RNase III

1 RNase III catalyzed hydrolysis is slower at low pH, perm

2 RNase III cleaves a hairpin structure within the arfA-co

3 RNase III enzyme Drosha interacts with DGCR8 to form the

4 RNase III family enzymes, which are perhaps the most wid

5 RNase III family members also exhibit a "catalytic" doma

6 RNase III is a global regulator of gene expression in Es

7 RNase III is a key enzyme in the pathways of RNA degrada

8 RNase III is subject to multiple levels of control, refl

9 RNase III proteins recognize double-stranded RNA structu

10 RNase III recognizes its substrates and selects the scis

11 RNase III-deficient cells are hypersensitive to high iro

12 RNase III[D45A], RNase III[D45E], and RNase III[D45N] ex

13 RNase III[D45E] activity is partially rescued by Mn(2+).

14 RNase III[E38A], RNase III[E65A], and RNase III[E100A] a

15 here an RNA, derived from the T7 phage R1.1 RNase III substrate, that is resistant to cleavage in vi

16 In the presence of Mg(2+) RNase III[DeltadsRBD] is less efficient than the wild-ty

17 pecific knockout of Dicer, a gene encoding a RNase III endonuclease essential for microRNA (miRNA) pr

18 e biochemical properties of A. aeolicus (Aa)-RNase III and the reactivity epitopes of its substrates

19 Aa-RNase III cleavage of the pre-16S substrate is blocked b

20 atalytic activity of purified recombinant Aa-RNase III exhibits a temperature optimum of approximatel

21 that a conserved glutamine (Q157) in the Aa-RNase III dsRNA-binding domain (dsRBD) directly interact

22 tion-related mRNAs by the S. coelicolor AbsB/RNase III enzyme occurs largely by ribonucleolytic cleav

23 e of the nuclease domain of Aquifex aeolicus RNase III, the E41, D114, and E117 side chains of E. col

24 transcriptomes of S. coelicolor M145 and an RNase III (rnc)-null mutant of that strain.

25 g Dicers, QDE-2, the exonuclease QIP, and an RNase III domain-containing protein, MRPL3.

26 rough directed cleavage of transcripts by an RNase III enzyme, Rnt1p.

27 ed in S. antibioticus may be processed by an RNase III-like activity, transcripts originating from Pp

28 talyzes editing in T. brucei and contains an RNase III motif that suggests nuclease function.

29 For this study, we created an RNase III null mutant of Streptococcus pyogenes and its

30 Dicer, an RNase III endonuclease, is an essential component of the

31 amining the effect of knocking out Dicer, an RNase III enzyme required for miRNA and small interferin

32 Using conditional knock-out of Dicer, an RNase III enzyme required for miRNA maturation, previous

33 though it is clear that miRNAs and Dicer, an RNase III enzyme that is central to the production of ma

34 Dicer, an RNase III enzyme, initiates RNA interference by processi

35 NAs) in vivo through the action of Dicer, an RNase III-family enzyme.

36 Loss of Dicer, an RNase III-like enzyme critical in microRNA biogenesis, c

37 In support of this, Dicer, an RNase III-like enzyme that controls the maturation of mi

38 DICER1, an RNase III endonuclease encoded by Dicer1, is required fo

39 Microprocessor contains Drosha, an RNase III endonuclease, and DGCR8, a gene deleted in DiG

40 Streptomyces coelicolor absB gene encodes an RNase III family endoribonuclease and is normally essent

41 Primer extension with RNA from an RNase III null mutant of Streptomyces coelicolor M145 an

42 at editosomes with KREPB2, which also has an RNase III motif, specifically cleave cytochrome oxidase

43 the expression of the rpsO-pnp operon in an RNase III (rnc) mutant of Streptomyces coelicolor.

44 and RpoS protein levels are increased in an RNase III mutant strain with or without the sRNAs, sugge

45 hat PNPase expression is autoregulated in an RNase III-dependent manner in S. coelicolor.

46 Dicer is an RNase III endonuclease which processes miRNA precursors

47 DICER1 is an RNase III endoribonuclease central to miRNA biogenesis,

48 Dicer is an RNase III enzyme essential for the maturation of the maj

49 Drosha is an RNase III enzyme that was recently implicated in miRNA p

50 Dicer is an RNase III-family nuclease that initiates RNA interferenc

51 Here, we present the crystal structure of an RNase III-product complex, the first catalytic complex o

52 Rnc70, an RNase III mutant that binds but does not cleave rIII, al

53 A contains, in this order: the NUTL site, an RNase III-sensitive hairpin and the N ribosome-binding s

54 These findings support an RNase III mechanism of action in which the catalytic dom

55 RNase III[D45A], RNase III[D45E], and RNase III[D45N] exhibit negligible activities, regardles

56 While RNase III[E41A] and RNase III[D114A] have K(Mg) values that are approximatel

57 t is shown here that the RNase III[E41A] and RNase III[D114A] mutants exhibit catalytic activities in

58 RNase III[E38A], RNase III[E65A], and RNase III[E100A] also require higher Mg(2+) concentratio

59 suring co-ordination of 16S rRNA folding and RNase III processing that results in production of prope

60 The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base

61 In fact, by preventing N autoregulation, RNase III activates N gene translation at least 200-fold

62 Bacterial RNase III orthologs cleave their substrates in a highly

63 Bacterial RNase III orthologs exhibit the simplest structures, wit

64 n on a post-catalytic complex of a bacterial RNase III bound to the cleaved minimal substrate.

65 (RNase III) family represented by bacterial RNase III and eukaryotic Rnt1p, Drosha and Dicer.

66 t role in substrate recognition by bacterial RNase III.

67 -nt siRNA-like species produced by bacterial RNase III.

68 gous RNase III domains from either bacterial RNase III enzymes or Giardia Dicer.

69 led a homodimer resembling that of bacterial RNase III but extended by a unique N-terminal domain, an

70 tive determinants of reactivity of bacterial RNase III substrates.

71 es coelicolor encodes a homolog of bacterial RNase III.

72 interest, the structurally simpler bacterial RNase III serves as a paradigm for the entire family.

73 For mechanistic studies, bacterial RNase III has been a valuable model system for the famil

74 ar) concentrations of ethidium bromide block RNase III[DeltadsRBD] cleavage of substrate, which is si

75 he Tetrahymena genome, two that contain both RNase III and RNA helicase motifs, Dicer 1 (DCR1) and DC

76 Bs-RNase III was found to cleave precursor scRNA at two sit

77 t of the RNase III-like endoribonuclease, Bs-RNase III, in scRNA processing.

78 Neither Bs-RNase III nor RNase M5, the two known narrow-specificity

79 Here we show that Bs-RNase III cleaves primarily at the 5' cleavage site and

80 no acids that are essential for catalysis by RNase III and Dicer are missing from the RNase III domai

81 stand substrate recognition and catalysis by RNase III, we examined steady-state and pre-steady-state

82 tudies on the mechanism of dsRNA cleavage by RNase III have focused mainly on the enzymes from mesoph

83 and Pasteurella multocida are all cleaved by RNase III as predicted, whereas the hairpin from Neisser

84 tains a hairpin structure that is cleaved by RNase III to produce a non-stop transcript.

85 ii and Salmonella typhimurium are cleaved by RNase III when expressed in E. coli.

86 dsRNA elimination by RNase III treatment prior to DRIPc-seq allowed the genom

87 ssed from viral replication intermediates by RNase III-like enzyme Dicer guide sequence-specific anti

88 dy, we show that cleavage of the N leader by RNase III does not inhibit antitermination but prevents

89 dation by an additional pathway, mediated by RNase III, which, in contrast to the RNase E-mediated pa

90 involves cleavage of structured precursor by RNase III-like endonucleases.

91 R1.1[CL3B] RNA is recognized by RNase III in the same manner as R1.1 RNA, as revealed by

92 es from other bacteria are also regulated by RNase III and tmRNA.

93 ADI1 is controlled post-transcriptionally by RNase III cleavage.

94 as a major antideterminant in S. cerevisiae RNase III activity, and suggest a rationale for their ap

95 nditions in the absence of the S. cerevisiae RNase III ortholog Rnt1p or of the nuclear exosome compo

96 dsRNA substrates of Saccharomyces cerevisiae RNase III (Rnt1p) are capped by tetraloops with the cons

97 he structure of the Saccharomyces cerevisiae RNase III (Rnt1p) postcleavage complex and explain why R

98 t1p, the only known Saccharomyces cerevisiae RNase III double-stranded RNA endonuclease, plays import

99 The Saccharomyces cerevisiae RNase III enzyme Rnt1p preferentially binds to double-st

100 e in assays employing purified S. coelicolor RNase III.

101 e E41, D114, and E117 side chains of E. coli RNase III are expected to be coordinated to a divalent m

102 Some E. coli RNase III substrates contain an internal loop, in which

103 E. coli RNase III-dependent cleavage events can regulate gene ex

104 is resistant to cleavage in vitro by E.coli RNase III but retains comparable binding affinity.

105 It has been proposed that E.coli RNase III can function in a non-catalytic manner, by bin

106 ns in their catalytic sites--inhibits E.coli RNase III cleavage of R1.1 RNA.

107 E.coli RNase III is a member of a structurally distinct superfa

108 E.coli RNase III requires a divalent metal ion for activity, wi

109 A minimal substrate of E.coli RNase III, muR1.1 RNA, was characterized and used to def

110 cidic amino acids, which in Escherichia coli RNase III are E38, E41, D45, E65, E100, D114, and E117.

111 ws that a truncated form of Escherichia coli RNase III lacking the dsRBD (RNase III[DeltadsRBD]) can

112 Escherichia coli RNase III participates in the maturation of the ribosoma

113 processing reactivities of Escherichia coli RNase III substrates are determined in part by the seque

114 Here we report the use of Escherichia coli RNase III to cleave double-stranded RNA (dsRNA) into end

115 munoprecipitated dsRNA from Escherichia coli RNase III WT and mutant strains were deep-sequenced.

116 he generation and use of an Escherichia coli RNase III-prepared human kinesin/dynein esiRNA library t

117 NMD can also complement RNase III-mediated nuclear degradation of unspliced RPS2

118 DICER is a cytoplasmic RNase III enzyme that not only cleaves precursor miRNAs

119 The next cleavage employs the cytoplasmic RNase III Dicer producing miRNA duplexes [3, 4].

120 RNase III[D45A], RNase III[D45E], and RNase III[D45N] exhibit negligible

121 Hence, the degenerate RNase III domain and a newly identified domain are criti

122 precursor substrates by the Drosha and Dicer RNase III enzymes.

123 istinct ~20S editosomes contains a different RNase III-type endonuclease, 1 of 3 related proteins and

124 tional processing of transcripts by directed RNase III (Rnt1p) cleavage, were shown to provide predic

125 Drosha RNase III catalyzes the first excision event, the releas

126 scherichia coli RNase III lacking the dsRBD (RNase III[DeltadsRBD]) can accurately cleave small proce

127 RNase III[E38A], RNase III[E65A], and RNase III[E100A] also require highe

128 Escherichia coli (Ec) RNase III catalytic activity is known to increase during

129 nition determinant for Escherichia coli (Ec) RNase III substrates.

130 In fact, Tm-RNase III cleaves an Ec-RNase III substrate with identical specificity and is in

131 d by antideterminant bp that also inhibit Ec-RNase III.

132 This study shows that Ec-RNase III is phosphorylated on serine in vitro by purifi

133 sequence is similar to that observed with Ec-RNase III substrates.

134 export membrane protein), and rnc (encoding RNase III) genes in a secF-nuoF-lepB-rnc cluster.

135 interactions between ybeY and rnc (encoding RNase III), ybeY and rnr (encoding RNase R), and ybeY an

136 double-strand RNA-specific endoribonuclease RNase III.

137 that the narrow-specificity endoribonuclease RNase III and the 5' exonuclease RNase J1 are not essent

138 The endoribonuclease RNase III cleaves double stranded RNAs, which can be for

139 oscopy and a human kinesin endoribonucleases RNase III-prepared short interfering RNA (esiRNA) librar

140 e deleted Dicer1, which encodes an essential RNase III enzyme for miRNA biogenesis, in murine CCAAT/e

141 tic residues conserved throughout eukaryotic RNase III enzymes.

142 been well characterized, but how eukaryotic RNase IIIs work is less clear.

143 ide a framework for understanding eukaryotic RNase IIIs.

144 eins, but are typified by mutually exclusive RNase III endonucleases with distinct cleavage specifici

145 her the catalytic properties of extremophile RNases III nor the structures and reactivities of their

146 Finally, RNase III[DeltadsRBD] is sensitive to specific Watson-Cr

147 ative constraints that would incur following RNase III processing of a genomic hairpin.

148 able to repress zorO in a strain deleted for RNase III, indicating that repression requires cleavage

149 the models suggest a stepwise mechanism for RNase III to execute the phosphoryl transfer reaction.

150 ation results to identify new substrates for RNase III cleavage.

151 -type E. coli and from strains defective for RNases III and E, two RNases reported to be involved in

152 Indeed CaDcr1, the sole functional RNase III enzyme in C. albicans, has additional function

153 The sequences of 79 fungal RNase III substrates were inspected to identify addition

154 s were used to identify potential new genes, RNase III cleavage sites and the direct or indirect cont

155 previously reported structures of homologous RNase III domains from either bacterial RNase III enzyme

156 We examine here how RNase III itself is regulated in response to growth and

157 ted from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface

158 protein that contains two ribonuclease III (RNase III) domains, the domain that harbors the active s

159 e such protein family, the ribonuclease III (RNase III) endonucleases, includes Rnt1, which functions

160 Ribonuclease III (RNase III) enzymes are a family of double-stranded RNA (

161 The Dicer ribonuclease III (RNase III) enzymes process long double-stranded RNA (dsR

162 Members of the ribonuclease III (RNase III) family are double-stranded RNA (dsRNA) specif

163 produced by members of the ribonuclease III (RNase III) family represented by bacterial RNase III and

164 Ribonuclease III (RNase III) is a conserved, gene-regulatory bacterial end

165 cessing are members of the ribonuclease III (RNase III) superfamily, which are highly conserved in eu

166 Escherichia coli ribonuclease III (RNase III; EC 3.1.24) is a double-stranded(ds)-RNA-speci

167 These data implicate RNase III recognition of viral RNA as an antiviral defen

168 tions, defined by four RNA binding motifs in RNase III and three protein-interacting boxes in dsRNA,

169 is there information on EB-binding sites in RNase III substrates.

170 k base-pair substitutions which also inhibit RNase III.

171 at a specific position in the pb to inhibit RNase III binding is due to the purine 2-amino group, wh

172 recognition antideterminants, which inhibit RNase III binding.

173 g double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes.

174 Although crRNA 3' end formation involves RNase III and trans-encoded tracrRNA, as in other type I

175 Editosomes with the KREN1 or KREN2 RNase III type endonucleases specifically cleave deletio

176 dthrough transcript requires mutants lacking RNase III, we detect readthrough transcripts in wild-typ

177 4, two Arabidopsis thaliana chloroplast Mini-RNase III-like enzymes sharing 75% amino acid sequence i

178 A model is proposed whereby one or more RNase III-type endonucleases mediate the initial binding

179 In Drosophila melanogaster, the multidomain RNase III Dicer-1 (Dcr-1) functions in tandem with the d

180 mRNA preparation from JSE1880 using a mutant RNase III protein that binds to transcripts but does not

181 ei which contains a degenerate, noncatalytic RNase III domain.

182 DROSHA is a nuclear RNase III enzyme responsible for cleaving primary microR

183 icroprocessor, which consists of the nuclear RNase III Drosha and the double-stranded RNA-binding dom

184 The nuclear RNase III Drosha catalyzes the first processing step tog

185 at1p, the nuclear exosome and by the nuclear RNase III endonuclease Rnt1p to prevent undesired expres

186 ear RNA-binding protein HYL1 and the nuclear RNase III enzyme DCL1 in processing of primary miRNA (pr

187 ge of the primary transcripts by the nuclear RNase III enzyme Drosha.

188 ng sequences were affected in the absence of RNase III.

189 The broad cellular actions of RNase III family enzymes include ribosomal RNA (rRNA) pr

190 Optimal activity of RNase III[DeltadsRBD] is observed at low salt concentrat

191 The biochemical behavior of RNase III of the hyperthermophilic bacterium Thermotoga

192 oth phenotypes are suppressed by deletion of RNase III.

193 tes that the db is a positive determinant of RNase III recognition, with the canonical UA/UG sequence

194 e C-terminal dsRNA-binding domain (dsRBD) of RNase III, indicating that EB perturbs substrate recogni

195 s, and fungi, members of the Dicer family of RNase III-related enzymes process double-stranded RNA (d

196 process requiring RNase E but independent of RNase III, the RNA chaperone Hfq, and the regulatory pep

197 The mechanism of EB inhibition of RNase III is not known nor is there information on EB-bi

198 cal role in lambda development, the level of RNase III activity therefore serves as an important sens

199 of these findings are discussed in light of RNase III substrate function as a gene regulatory elemen

200 roximately 2.8-fold larger than the K(Mg) of RNase III (0.46 mM), the RNase III[E41A/D114A] double mu

201 are cleaved by Rnt1p, the yeast ortholog of RNase III, which creates an entry site for complete degr

202 ed in the absence of the yeast orthologue of RNase III Rnt1p or of the 5' --> 3' exonucleases Xrn1p a

203 nce between nutL and nutR is the presence of RNase III processing sites (rIII) located immediately pr

204 We discovered that down-regulation of RNase III activity occurs during both stresses and is de

205 clease-binding proteins in the regulation of RNase III activity.

206 te, reflecting the growth rate regulation of RNase III expression itself.

207 eviously unknown function, is a regulator of RNase III cleavages.

208 thout the sRNAs, suggesting that the role of RNase III in this context is to reduce the translation o

209 , the domain that harbors the active site of RNase III and Dicer enzymes.

210 e presence of hitherto-undiscovered sites of RNase III cleavage of the pnp transcript.

211 Here, we present three crystal structures of RNase III in complex with double-stranded RNA, demonstra

212 rocessing, gained from structural studies of RNase III, is reviewed.

213 trans-acting sRNAs that can be substrates of RNase III.

214 nic regions appeared to be direct targets of RNase III.

215 pre-rRNA processing is identical to that of RNase III in bacteria: to co-transcriptionally separate

216 nt evidence for cytoplasmic translocation of RNase III nucleases in response to virus in diverse euka

217 gene-loss events to generate the variety of RNase III enzymes found in modern-day budding yeasts.

218 ls in mutants defective in either RNase E or RNase III.

219 hydrolysis that can be extrapolated to other RNase III family members.

220 Prokaryotic RNase III enzymes have been well characterized, but how

221 ditional stem-loop and changes in a putative RNase III cleavage site in the flqB mutant.

222 KREPB1 with point mutations in the putative RNase III catalytic domain also blocked growth, in vivo

223 dependent, factors that dynamically regulate RNase III actions during normal cell growth.

224 Here we demonstrate that Drosha and related RNase III ribonucleases from all three domains of life a

225 Drosha is a member of the ribonuclease (RNase) III family that selectively processes RNAs with p

226 onuclease required in DNA repair, and Rnt1p (RNase III), an endoribonuclease required for RNA maturat

227 malian CNS, we used mice in which the second RNase III domain of Dicer was conditionally floxed.

228 n primary transcripts through two sequential RNase III-mediated cleavages; Drosha cleaves near the ba

229 Each editosome requires the single RNase III domain in each endonuclease for catalysis.

230 The tandem RNase III (RIII) domains of Dcr-1 form an intramolecular

231 Dicer-2 contains C-terminal RNase III domains that mediate RNA cleavage and an N-ter

232 NAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD/H-box

233 amount of dsRNA is formed in the cell, that RNase III degrades or processes these dsRNAs, and that d

234 We find that RNase III rapidly and efficiently cleaved the transcript

235 Previously, we have shown that RNase III uses two catalytic sites to create the 2-nucle

236 This study also shows that RNase III recognition and cleavage of substrate can be u

237 The RNase III endonuclease Dicer plays a key role in generat

238 The RNase III enzyme Dicer processes RNA into siRNAs and miR

239 The RNase III enzyme Drosha initiates microRNA (miRNA) bioge

240 The RNase III gene of Streptomyces coelicolor, which was dis

241 The RNase III polypeptide contains an N-terminal catalytic (

242 sha processing is currently unique among the RNase III enzymes.

243 8 is an RNA-binding protein that assists the RNase III enzyme Drosha in the processing of microRNAs (

244 These pre-miRNAs are cleaved by the RNase III Dicer to generate mature miRNAs that direct th

245 miRNA is processed from the pre-miRNA by the RNase III Dicer.

246 erminal tRNA-derived fragments (tRFs) by the RNase III endonuclease Dicer, we show that several RNase

247 partially double-stranded precursors by the RNase III endonucleases Drosha and Dicer, thereby genera

248 al microRNA biogenesis pathway driven by the RNase III enzymes Drosha and Dicer, an unexpected variet

249 taining RNA polymerase II transcripts by the RNase III enzymes Drosha in the nucleus and Dicer in the

250 onical miRNAs is sequentially cleaved by the RNase III enzymes, Drosha and Dicer, which generate 5' m

251 gions whose expression was influenced by the RNase III gene deletion.

252 ce with a conditional deletion of Dicer, the RNase III endonuclease that produces mature microRNAs in

253 and cd19-Cre-mediated deletion of Dicer, the RNase III enzyme critical for generating mature miRNAs.

254 short interfering RNA (siRNA) by DICER, the RNase III enzyme that initiates RNAi in human cells.

255 presence and absence of Dicer or Drosha, the RNase III nucleases responsible for generating small RNA

256 by RNase III and Dicer are missing from the RNase III domains in RNC1.

257 onal roles of two divalent metal ions in the RNase III catalytic mechanism.

258 functions by interacting with a site in the RNase III catalytic region, that expression of YmdB is t

259 ations in DICER1, an endoribonuclease in the RNase III family that is essential for processing microR

260 eles with single amino acid mutations in the RNase III motif had similar consequences.

261 lly functional asRNAs were identified in the RNase III mutant strain and are encoded primarily opposi

262 levels were higher or detectable only in the RNase III mutant strain.

263 tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae

264 r than the K(Mg) of RNase III (0.46 mM), the RNase III[E41A/D114A] double mutant has a K(Mg) of 39 mM

265 Deficient expression of the RNase III DICER1, which leads to the accumulation of cyt

266 -stranded RNA-binding protein partner of the RNase III enzyme Drosha.

267 Rnt1p, a member of the RNase III family of dsRNA endonucleases, is a key compon

268 ge is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcript

269 of miR166g, and require the activity of the RNase III helicase DCL1.

270 Deletion or mutation of the RNase III motif abolished this activity.

271 ous work demonstrated the involvement of the RNase III-like endoribonuclease, Bs-RNase III, in scRNA

272 The implications of these findings on the RNase III catalytic mechanism are discussed.

273 Essential insight on the RNase III mechanism of dsRNA cleavage has been provided

274 rabidopsis indicate that three proteins, the RNase III DICER-Like1 (DCL1), the dsRNA-binding protein

275 It is shown here that the RNase III[E41A] and RNase III[D114A] mutants exhibit cat

276 for siRNA-directed mRNA cleavage, though the RNase III activity of Dicer-2 is not required.

277 Our results suggest two models whereby the RNase III enzymes of a fungal ancestor, containing both

278 y miRNAs (pri-miRNAs) in connection with the RNase III enzyme Drosha.

279 ing in the context of a heterodimer with the RNase III-domain protein RNC1.

280 Seven of these were within the RNase III domain, and two were in the C-terminal region

281 During viral infection, the RNase-III-type endonuclease Dicer cleaves viral double-s

282 T. maritima (Tm) RNase III catalytic activity exhibits a broad optimal te

283 Tm-RNase III cleavage of substrate is optimally supported b

284 stem sequences are efficiently cleaved by Tm-RNase III at sites that are consistent with production i

285 In fact, Tm-RNase III cleaves an Ec-RNase III substrate with identic

286 latory RNAs via consecutive cleavages by two RNase III enzymes, Drosha and Dicer.

287 An involvement of two RNase III-containing core editing complex (L-complex) pr

288 ecent report in Cell reveals how Dicer's two RNase III domains collaborate during dsRNA processing an

289 and cold shock adaptation are dependent upon RNase III cleavage of an rpsO-pnp intergenic hairpin.

290 Biogenesis of CoV svRNAs was RNase III, cell type, and host species independent, but

291 s in the db to inhibit cleavage by weakening RNase III binding indicates that the db is a positive de

292 e Arg95 guanidinium group, thereby weakening RNase III engagement of product.

293 While RNase III[E41A] and RNase III[D114A] have K(Mg) values t

294 +) concentrations for optimal activity, with RNase III[E100A] exhibiting the largest K(Mg).

295 l to the nucleus and becomes associated with RNase III DROSHA and the RNA helicase p68.

296 and addition, 2 RNA ligases, 2 proteins with RNase III-like domains, and 6 proteins with predicted ol

297 Five related proteins with RNase III-like motifs also contain a U1-like zinc finger

298 which is similar to the inhibition seen with RNase III and is indicative of an intercalative mode of

299 Comparison of PARE peaks in strains with RNase III present or absent showed that, in addition to

300 ovel polypeptides, among which were two with RNase III, one with an AP endo/exonuclease and one with