ライフサイエンスコーパス: RP-HPLC

1 RP HPLC peaks are represented by bands of different inte

2 RP-HPLC and LC-MS/MS profil1 for soybean, flaxseed and o

3 RP-HPLC and PAGE analyses of the fully deprotected AUCCG

4 RP-HPLC peptide profiling revealed that the AcPs activit

5 RP-HPLC resolves the isoforms of purified apoA-I and apo

6 RP-HPLC was used to quantify DHA, MGO and hydroxymethylf

7 RP-HPLC, LC-MS, and NMR experiments indicate that the pr

8 RP-HPLC-DAD-FLU separation enabled us to identify 20 der

9 RP-HPLC-ESI-MS/MS results showed different peptides occu

10 ification of the metabolite collected from a RP HPLC column further separated the radiolabeled materi

11 A RP-HPLC method, developed for the separation and quantif

12 e report the development and validation of a RP-HPLC method with fluorometric detection of derivatize

13 he quantification was carried out by using a RP-HPLC method, based on a pre-column derivatization wit

14 An RP-HPLC method was developed which permitted the quality

15 colorants were separated and analyzed by an RP-HPLC/DAD system.

16 We present an RP-HPLC method for the determination of the octanol-wate

17 We present an RP-HPLC method, for the determination of logPoct values

18 ed penicillins, which were analysed using an RP-HPLC method, validated according to the European Deci

19 and its authenticity was determined using an RP-HPLC method.

20 obtained after limited tryptic digestion and RP-HPLC.

21 (PsTx), was purified by cation exchange and RP-HPLC and has a molecular mass of about 24 kDa.

22 combining size-exclusion, ion-exchange, and RP-HPLC chromatographies.

23 omatography, OFFGEL isoelectric focusing and RP-HPLC.

24 estion products were analyzed by RP-HPLC and RP-HPLC-ESI-MS/MS.

25 used a newly developed radioimmunoassay and RP-HPLC to detect mppOFQ/N(160-187) in mouse hypothalami

26 DSC, CD, and NMR) and bioanalytical (SEC and RP-HPLC-MS) methods.

27 using a combination of mass spectrometry and RP-HPLC separation and bioassays.

28 d by standard analytical techniques, such as RP-HPLC, ESI-MS, and NMR spectroscopy.

29 The combination of techniques such as RP-HPLC-UV, GFAAS and MALDI-TOF-MS allowed the identific

30 12b were cleaved by acidolysis, purified by RP HPLC, and isolated in high yield and purity, demonstr

31 tion of the epimers has been accomplished by RP-HPLC, allowing full characterization and incorporatio

32 tion of CA down to 0.2%, w/w was achieved by RP-HPLC-DAD using aqueous acetonitrile elution solvent (

33 e fractionated and the fractions analysed by RP-HPLC-ICP-MS coupled with ESI-Q-TOF-MS.

34 igested with MMP-20 and Klk4 and analyzed by RP-HPLC and by mass spectrometry.

35 The digestion products were analyzed by RP-HPLC and RP-HPLC-ESI-MS/MS.

36 e complex peptide mixture can be analyzed by RP-HPLC; however, only the level of total clipping is id

37 xy-2'-deoxyguanosine (8-OHdG) as assessed by RP-HPLC (MS).

38 r disease versus physiological conditions by RP-HPLC chromatography, ProteinChip technology, and QSta

39 N-acetylserotonin (NAS) was detected by RP-HPLC in human skin extracts.

40 netic variants were successfully detected by RP-HPLC.

41 vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation

42 of 1.551-196.89 mg/g of PRF as determined by RP-HPLC.

43 roteins behaved differently when examined by RP-HPLC chromatography and surface-enhanced laser desorp

44 otential furfural compounds were examined by RP-HPLC-DAD in 20 commercial milk-based powdered infant

45 tion of the disulfide-linked DM1 followed by RP-HPLC allowed estimation of purity of MAb-linked DM1 a

46 We found limited proteolysis followed by RP-HPLC-MS to be less time-consuming for sample preparat

47 y potential, FIV and FV were fractionated by RP-HPLC and then analyzed by LC-MS/MS to identify peptid

48 The active fraction was purified further by RP-HPLC, the chemoattractant protein appearing as a sing

49 roducts of AANAT activity were identified by RP-HPLC with fluorimetric detection in human skin and in

50 15-kDa C-terminal fragment was identified by RP-HPLC, which bound to CaM-agarose in the presence but

51 gallic and ellagic acids were identified by RP-HPLC-DAD, also coupled to off-line matrix assisted la

52 T was realized within 35 min as indicated by RP-HPLC and verified later by thermodynamically driven r

53 embly by method (A), the linkers isolated by RP-HPLC at pH approximately 2.2 were added to D at neutr

54 A byproduct was isolated by RP-HPLC from the resin-cleaved crude product of each ben

55 thionine, can be identified at ppb levels by RP-HPLC-ICPMS, since standards are readily available.

56 e mitochondrial ribosomal proteins (MRPs) by RP-HPLC is described.

57 lected bioactive compounds were performed by RP-HPLC.

58 PST-2), subsequent separation of products by RP-HPLC, and mass spectrometry analysis.

59 oba L. and pharmaceutical ginkgo products by RP-HPLC, based on the theory of terpene trilactones ioni

60 ever, the separation selectivity provided by RP-HPLC has been limited to the hydrophobicity-based res

61 id phase peptide methodology and purified by RP-HPLC.

62 and the peptides were partially purified by RP-HPLC.

63 sing standard Fmoc chemistry and purified by RP-HPLC; all intermediate and final products were verifi

64 r 1Dx5 and 1Dy10 (L8) and were quantified by RP-HPLC and GP-HPLC.

65 [3H]CM-DTT separated from excess reagent by RP-HPLC.

66 Proteins separated by RP-HPLC have potential inhibitory and antioxidant activi

67 ed from each synthesis and were separated by RP-HPLC to yield enantiomerically pure 6-9.

68 ioxidant activities of proteins separated by RP-HPLC were observed in glutein-2 and albumin, respecti

69 with [3H]CM-DTT, peptides were separated by RP-HPLC, and aliquots of each fraction were counted for

70 ere purified from bovine liver, separated by RP-HPLC, and identified by 2D PAGE techniques and immuno

71 reomers of compounds 24a-f were separated by RP-HPLC.

72 eadily accessible for complete separation by RP-HPLC and detailed characterization by in-line MS anal

73 ut quantitative analysis of gliadin types by RP-HPLC to verify its correlation with the amount of tox

74 s(1)-Cys(7) disulfide, which was verified by RP-HPLC and MALDI-ToF-MS.

75 stion products by LC-MSMS, SDS-PAGE, and C18 RP-HPLC monitored with fluorescence and UV detectors.

76 First a C4 RP-HPLC column was used, then a C18 RP Microbore column

77 hase high-performance liquid chromatography (RP HPLC).

78 sequentially fractionated by chromatography (RP-HPLC) and each fraction was tested for antimutagenic

79 hase high-performance liquid chromatography (RP-HPLC) analysis of mAb A samples showed three distinct

80 hase high-performance liquid chromatography (RP-HPLC) analysis, accumulation of terephthalic acid (Ta

81 hase high-performance liquid chromatography (RP-HPLC) and its applications are described.

82 d phase high pressure liquid chromatography (RP-HPLC) and mass spectrometry (MS) were used for identi

83 hase-high performance liquid chromatography (RP-HPLC) and matrix assisted laser desorption ionisation

84 hase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption/ionization

85 hase-high performance liquid chromatography (RP-HPLC) and quantified in a primer extension assay from

86 hase high-performance liquid chromatography (RP-HPLC) and separated by capillary zone electrophoresis

87 hase-high performance liquid chromatography (RP-HPLC) and then subjected to chemometric evaluation.

88 hase high-performance liquid chromatography (RP-HPLC) and tracking the presence of degradation produc

89 hase high-performance liquid chromatography (RP-HPLC) assays developed in our laboratory.

90 hase high performance liquid chromatography (RP-HPLC) coupled off-line with CS-MAS for the identifica

91 hase high-performance liquid chromatography (RP-HPLC) coupled with electrospray ionization mass spect

92 hase-high-performance liquid chromatography (RP-HPLC) demonstrated robust lung, liver, and spleen BMM

93 hase high-performance liquid chromatography (RP-HPLC) eluent fractions, either before or after lyophi

94 hase high-performance liquid chromatography (RP-HPLC) led to the production of several peptide fracti

95 hase high-performance liquid chromatography (RP-HPLC) method was developed for detection and quantifi

96 d-phase-high-pressure liquid chromatography (RP-HPLC) separation and parallel ICPMS and electrospray

97 hase high performance liquid chromatography (RP-HPLC) to determine honeys' phenolic profiles.

98 C) and reversed-phase liquid chromatography (RP-HPLC) using a new spiked gradient profile is presente

99 hase high-performance liquid chromatography (RP-HPLC) was developed and performed for verification of

100 hase high-performance liquid chromatography (RP-HPLC) were found to be complementary in the study of

101 hase high performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detector was used t

102 hase high-performance liquid chromatography (RP-HPLC), and electrospray ionization mass spectrometry

103 hase high performance liquid chromatography (RP-HPLC), followed by post-column addition of lithium sa

104 d-phase high-pressure liquid chromatography (RP-HPLC), gel electrophoresis (SDS-PAGE), or strong cati

105 hase high-performance liquid chromatography (RP-HPLC), is active in a bioassay using A. brasiliana; i

106 hase high-performance liquid chromatography (RP-HPLC), using a new column of faster resolution was va

107 hase high-performance liquid chromatography (RP-HPLC).

108 hase high performance liquid chromatography (RP-HPLC).

109 hase-high performance liquid chromatography (RP-HPLC).

110 e phase high pressure liquid chromatography (RP-HPLC).

111 hase high performance liquid chromatography (RP-HPLC).

112 e separated by reverse-phase chromatography (RP-HPLC-UV).

113 We combined RP-HPLC and mass spectrometry to identify secreted or sh

114 cted to a much higher precision than current RP-HPLC models.

115 matography coupled to diode array detection (RP-HPLC/DAD) was developed using a fused core pentafluor

116 dic) compounds has been studied on different RP-HPLC columns in buffers of varying ionic strength.

117 eled and labeled counterparts coelute during RP-HPLC separation but exhibit a sufficient mass differe

118 the tumor-specific CTLs, an aliquot of each RP-HPLC fraction was added to the autologous, B-lymphobl

119 a combination of capillary electrophoresis, RP-HPLC, and NMR spectroscopy, we show that WbpB and Wbp

120 Finally, RP-HPLC based fractionation of POCIS extracts and testin

121 ar weight (M(r)) in fractions collected from RP-HPLC.

122 PEG and PEGylated compounds were eluted from RP-HPLC, and the HPLC stream was mixed with diethylmethy

123 To this aim a previous gradient RP-HPLC method with DAD detection was modified and valid

124 In comparison to gradient RP-HPLC, the resolving power of the described CEC method

125 is study as a marker in reverse-phased HPLC (RP-HPLC) analyses of adenovirus for the quantitation of

126 No peaks appeared in RP-HPLC chromatograms of globulin for either species.

127 t for all molecular interactions involved in RP-HPLC.

128 IP RP HPLC is also suitable for the separation and purifica

129 IP RP HPLC provides a fast and reliable alternative to clas

130 RNA purified by IP RP HPLC exhibits improved stability.

131 e high performance liquid chromatography (IP RP HPLC) is presented as a new, superior method for the

132 The versatility of IP RP HPLC for RNA analysis is demonstrated.

133 IP-RP HPLC, MALDI-TOF MS, exonuclease digests, and a simple

134 mer pairs were separated and collected by IP-RP HPLC.

135 e high-performance liquid chromatography (IP-RP HPLC) techniques were combined to determine the seque

136 Partial sequence information for IP-RP HPLC fractions was obtained from analyzing exonucleas

137 Clearly, QRT-PCR and IP-RP-HPLC are powerful and versatile tools for the detecti

138 e high-performance liquid chromatography (IP-RP-HPLC) and detected by UV spectroscopy as well as Orbi

139 e high-performance liquid chromatography (IP-RP-HPLC) procedure has been developed for nonisotopic de

140 uid Chromatography-Diode Array Detection (IP-RP-HPLC-DAD).

141 re than one splice variant was expressed, IP-RP-HPLC identified the specific homoduplex products, as

142 h-performance liquid chromatography (nano-IP-RP-HPLC).

143 The IP-RP-HPLC procedure specifically detects and quantifies S-

144 The protein-level RP-HPLC and the high-pH RP-HPLC peptide-level separation

145 /CIS-MS and APCI-MS procedures after on-line RP-HPLC separation.

146 gh performance liquid chromatography method (RP-HPLC) was established to quantify the peptides.

147 romatography in line with time-of-flight MS (RP-HPLC-TOF/MS) revealed adducts of +162 and +324 Da to

148 erythroleukemia cell line lysate, the IEF-NP RP HPLC produced improved resolution of low mass and bas

149 It is demonstrated that IEF-NP RP HPLC provides a viable alternative to the 2-D gel sep

150 d-phase HPLC in the second dimension (IEF-NP RP HPLC).

151 Using IEF-NP RP HPLC, approximately 700 bands were resolved in a pI r

152 In this work, it is demonstrated that NP RP HPLC with MALDI-TOFMS detection may serve as a rapid

153 Nonporous (NPS) RP-HPLC has been used to rapidly separate proteins from

154 With the aid of RP-HPLC and chiral SFC separation techniques, the new di

155 ed out in two peptide fractions (A and B) of RP-HPLC collected at retention time (RT) 24 and 28min re

156 CA of RP-HPLC results of the matured cheeses revealed the pres

157 This combination of RP-HPLC and CZE was able to uniquely resolve all of the

158 ography (HPLC) and an on-line combination of RP-HPLC and electrospray ionization mass spectrometry.

159 ulation method applied to the integration of RP-HPLC chromatograms allowed for the generation of a pe

160 , glutenins) has been determined by means of RP-HPLC, to assess the inter- and intra-batch variabilit

161 By means of RP-HPLC-ICPMS/ESMS, we investigated Capelin oil ( Mallot

162 After the third round of RP-HPLC, mass spectrometry was used to sequence individu

163 uilt-in peristaltic pump, enabled the use of RP-HPLC with gradient elution and up to 100% organic com

164 report details and experience of our use of RP-HPLC-CLND-MS to characterize and quantify small amoun

165 metabolite that migrated at 17.5-18.5 min on RP-HPLC.

166 le, and eluted as a single symmetric peak on RP-HPLC.

167 ter eluting [(99m)TcO]depreotide products on RP-HPLC were confirmed to be the anti and syn diastereom

168 The digests were separated on RP-HPLC and analyzed by MS/MS on Q-Tof Global mass spect

169 This paper presents a study on RP-HPLC determination of the content of indole compounds

170 -48 digestion fragments in bulk by SDS-PAGE, RP-HPLC, and MALDI-TOF proves that the previous pepsin e

171 Thus, an ion-pair RP-HPLC method for the simultaneous determination of lac

172 by the determination of furosine by ion-pair RP-HPLC-UV.

173 bsequent MALDI-TOF analysis of the partially RP-HPLC purified peptides yielded a fraction containing

174 ing the basic principles of gradient peptide RP-HPLC and solidify our collective efforts in acquiring

175 The protein-level RP-HPLC and the high-pH RP-HPLC peptide-level separations performed the best, id

176 diastereomers were separated by preparative RP-HPLC in 13% yield from D-Val.

177 I and Id were separated by preparative RP-HPLC.

178 pplications, we developed a simple and rapid RP-HPLC method based on isosbestic point detection.

179 peptides incidentally collected in the same RP-HPLC fraction were well resolved from the glycopeptid

180 ification was carried out by semipreparative RP HPLC followed by lyophilization and yielded a compoun

181 Sequential RP-HPLC fractionation of the active extracts produced bo

182 formic acid), even with highly inert silica RP-HPLC columns of normal dimensions.

183 le (ACN) SPE fraction and the most active SP RP-HPLC peptide fraction (SP RP-HPLC 25_F28), respective

184 e-high performance liquid chromatography (SP RP-HPLC).

185 most active SP RP-HPLC peptide fraction (SP RP-HPLC 25_F28), respectively.

186 fied following UPLC-ESI MS/MS analysis of SP RP-HPLC 25_F28.

187 nce liquid chromatography mass spectrometry (RP-HPLC/MS).

188 The RP-HPLC fraction with highest antioxidant activity conta

189 their preparation and purification, and the RP-HPLC recovery of the chain ligation intermediates.

190 s the main component, was carried out by the RP-HPLC method.

191 hemokine was demonstrated by identifying the RP-HPLC separated tryptic and endoprotease Glu-C peptide

192 The heterogeneities observed in the RP-HPLC have been determined to arise from unpaired cyst

193 Two predominant peaks were observed in the RP-HPLC profiles of all protein fractions.

194 the DPPH assay as well as injection into the RP-HPLC system containing a PFP (pentafluorophenyl) colu

195 One of the RP-HPLC fractions (B) showed the presence of 14 amino ac

196 dentical mass spectra, a comparison of their RP-HPLC chromatograms suggested that the two forms were

197 According to RP-HPLC experiments, the highest concentration of phenol

198 investigations indicate that in addition to RP-HPLC-ICPMS/ESI-MS a range of different derivatization

199 separation selectivity highly orthogonal to RP-HPLC.

200 Similar to RP-HPLC prediction studies, we anticipate future develop

201 Traditional RP-HPLC with approximately 3-mum particles has shown gen

202 This hydrolysate was separated by using RP-HPLC and three fractions with high antioxidant activi

203 Bottom-up characterization using RP-HPLC/MS peptide mapping and accurate mass measurement

204 for sustained release, was determined using RP-HPLC at neutral pH yielding analogues with shorter as

205 e resulting peptides were fractionated using RP-HPLC and analyzed by CE-MS yielding a total of 28538

206 igosaccharides from salts and proteins using RP-HPLC with a formic acid/acetonitrile/water mobile pha

207 balt-containing peptides were purified using RP-HPLC.

208 arachidonic acid (AA) were quantified using RP-HPLC with charged aerosol detection in a range of com

209 tereomeric peptides could be separated using RP-HPLC in most cases.

210 ypsin, individual peptides were obtained via RP-HPLC and were assayed for monosaccharides by strong a

211 Compared with RP-HPLC MS analysis of the same mixture in terms of spee