ライフサイエンスコーパス: RPS6

1 in 1 (4E-BP1), but not ribosomal protein S6 (rpS6).

2 ion of mTOR signaling and phosphorylation of rpS6.

3 a ribosomal protein (rp)S6 kinase, S6K1, and rpS6.

4 ressed into soybean lines along with Rps4 or Rps6.

5 ation of Thr(389) and of the S6K1 substrate, RPS6.

6 4E-BP1 but also inhibited phosphorylation of rpS6.

7 or of parathyroid cell proliferation through rpS6.

8 to inhibition of the kinase as compared with rpS6.

9 sed phosphorylation of ribosomal protein S6 (rpS6), a downstream target of the mTOR pathway.

10 RPS6, a component of the mammalian target of rapamycin (

11 osophila melanogaster cells expressing human RPS6, a TORC1 effector whose phosphorylated form we dete

12 locked diurnal oscillations in eIF4E, 4EBP1, rpS6, Akt, and ERK1/2 phosphorylation and impaired memor

13 se (RPS6KB1), and phosphorylated S6 protein (RPS6), all of which mediate cell growth and proliferatio

14 Here, we focus on ribosomal protein S6 (rpS6), an mTOR effector not implicated previously in can

15 locked UNX-increased phosphorylation of both rpS6 and 4E-BP1.

16 ts and partially restored phosphorylation of RPS6 and 4EBP1.

17 We propose that the interaction between RPS6 and AtHD2B brings about a change in the chromatin s

18 Co-expression of RPS6 and AtHD2B caused a change in the location of both

19 HD2B caused a change in the location of both RPS6 and AtHD2B to one or several nucleolar spots.

20 RPS6 and AtHD2B were localized to the nucleolus.

21 In conclusion, the cloning of RPS6 and comparisons with RPS4 will contribute to a clos

22 The mTORC1 effectors, RPS6 and eIF4E, play distinct roles and are both necessa

23 chemical inhibitor, or knockdown of AKT1S1, RPS6 and EIF4EBP1 expression by small interfering RNA, m

24 mTORC1 signaling pathway, i.e. AKT, AKT1S1, RPS6 and EIF4EBP1, was upregulated in islets upon TGFBI

25 the phosphorylation of two S6K1 substrates, rpS6 and eukaryotic initiation factor 4B, was not repres

26 0S6K activation inhibited phosphorylation of RPS6 and IL-3-enhanced semaphorin-7A translation.

27 ivated MAPK signaling and phosphorylation of rpS6 and led to phosphorylation of GSK3beta-Ser(9), a no

28 nged environment, in vivo phosphorylation of RPS6 and p90S6K was enhanced in human airway compared wi

29 mediated by the resistance genes RPS2, RPS4, RPS6 and RPM1.

30 -NB-LRR disease resistance proteins RPS4 and RPS6 and with the negative immune regulator SRFR1 at a c

31 its role in ribosome assembly suggests that RPS6 (and by extension other ribosomal proteins) contrib

32 s by regulating p70S6K/ribosomal protein S6 (RPS6) and eukaryotic translation initiation factor 4E-bi

33 easome activity; phosphorylation of S6K1 and rpS6) and rapamycin-insensitive (phosphorylation of eEF2

34 t nsP2 associates with ribosomal protein S6 (RpS6) and that nsP2 is present in the ribosome-containin

35 the phosphorylation of ribosomal protein S6 (rpS6) and the amount of phosphorylated rpS6 bound to the

36 phorylation of the 40S ribosomal protein S6 (rpS6) and the eukaryotic translation initiation factor (

37 through activation of ribosomal protein S6 (RPS6) and the upstream kinase 90-kDa ribosomal S6 kinase

38 sed ERK1/2, MAPK-activated protein kinase 2, rpS6, and CREB phosphorylation in fetal Tbet(+)CD4(+) T

39 on of mTOR and its effectors 4E-BP1, p70S6K, rpS6, and eukaryotic initiation factor 4G.

40 ns with Myo5a, Prkra (PACT), Gnb2l1 (RACK1), Rps6, and Syt2 were confirmed by Western blot analysis.

41 As both RSK and rpS6 are known to be important for cell proliferation an

42 all inhibitory RNA knockdown of RPS6 defined RPS6 as a critical regulator of 5' TOP translation.

43 we addressed the role of phosphorylation of rpS6 as an effector of mTOR function in T cell developme

44 Our results show that phosphorylation of RPS6 as well as 4E-binding protein 1 (4EBP1) was reduced

45 hosphorylation of S6 kinase and its effector rpS6, as well as phosphorylation of the translational re

46 For the first time, we show that RPS6 associates with multiple mRNAs containing a 5' TOP

47 nd forskolin, lead to the phosphorylation of rpS6 at Ser235/Ser236 independently of the activation of

48 ls, phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 was mostly abrogated, and this BMK1-

49 Here we report the identification of RPS6 based on a forward-genetic screen and map-based clo

50 ating that the corresponding resistance gene RPS6 belongs to the TNL class.

51 n S6 (rpS6) and the amount of phosphorylated rpS6 bound to the translation initiation complex were in

52 nsP1 also was associated with RpS6, but other nonstructural proteins were not.

53 that protein phosphatase 1 (PP1) antagonizes rpS6 C terminus phosphorylation and cap binding in intac

54 Knockin rpS6(p-/-) mice, in which rpS6 cannot be phosphorylated because of substitution of

55 and Ser-244) whose modification potentiates rpS6 cap binding activity.

56 pression and the activity of MEK-ERK and S6K-RPS6 cascades but also displays a potent antiproliferati

57 inishes phosphorylation of eIF4B, eIF4E, and rpS6, critical components of the intracellular machinery

58 h specific small inhibitory RNA knockdown of RPS6 defined RPS6 as a critical regulator of 5' TOP tran

59 ChIP analysis suggests that RPS6 directly interacts with the rRNA gene promoter.

60 expressing a nonphosphorylatable variant of Rps6 display a reduced growth rate and a 40S biogenesis

61 liferation, in the renal tubules of Tsc1 and rpS6 double-mutant mice.

62 otein S6 kinase (S6k), ribosomal protein S6 (rpS6), eukaryotic elongation factor 2 (eEF2), and eukary

63 ther, we conclude that PKA can phosphorylate rpS6 exclusively at Ser235/Ser236 in vivo in pancreatic

64 Protoplasts overexpressing both AtHD2B and RPS6 exhibited down-regulation of pre-18 S rRNA synthesi

65 Depletion of RPS6 from primary effusion lymphoma (PEL) cells dramatic

66 of the mTOR signaling pathway that regulate rpS6 function.

67 Similar to RPS4 and other TNL genes, RPS6 generates alternatively spliced transcripts, althou

68 Two maize (Zea mays) rps6 genes were identified that encode polypeptides (30

69 The fact that RPS6 has a well-established nuclear function beyond its

70 des the translational machinery, implicating RPS6 in 5' TOP translation.

71 OS or NIH 3T3 cells phosphorylates mammalian RPS6 in a mitogen-dependent wortmannin- and rapamycin-se

72 tle is known about other possible role(s) of RPS6 in plants, besides being a component of the 40 S ri

73 adient, suggesting that nsP2 associates with RpS6 in the context of the whole ribosome.

74 Finding a role for RpS6 in the negative regulation of efferocytosis provide

75 Notably, knockin of a nonphosphorylatable rpS6 in these Tsc1-mutant mice exacerbated cystogenesis

76 presence of BMK1 and the phosphorylation of rpS6 in tumor-associated endothelial cells of blood vess

77 sed phosphorylation of ribosomal protein S6 (rpS6) in activated renal tubules.

78 d phosphorylation of ribosomal protein S6 (p-rpS6) in SNpc neurons, a readout of trkB activation.

79 K1) and its substrate, ribosomal S6 protein (rpS6), in quiescent 3T3 cells.

80 apamycin (mTOR) effectors 4EBP1, p70S6K, and rpS6, independent of HIF.

81 ther nonstructural proteins was required for RpS6 interaction with nsP2.

82 Here, we show that ribosomal protein S6 (RPS6) interacts with LANA.

83 Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subu

84 Ribosomal protein S6 (rpS6) is an essential component of the ribosome and is i

85 y, we demonstrate that ribosomal protein S6 (RPS6) is highly expressed in primary diffuse large B-cel

86 Ribosomal protein S6 (RPS6) is located in the mRNA binding site of the 40S sub

87 The ribosomal protein S6 (RPS6) is one of the well known downstream components of

88 Ribosomal protein S6 (rpS6) is phosphorylated in vivo by isoforms of p70 S6 pr

89 Nine RPS6 isoforms were resolved in a two-dimensional basic-u

90 This cAMP-dependent rpS6 kinase activity is also sensitive to PKI in vitro,

91 henotypes are not observed in cells in which Rps6 kinase activity is compromised.

92 d phosphorylation of the ERK1 and the 90-kDa rpS6 kinase p90(rsk).

93 he activation of the currently known in vivo rpS6 kinases via a pathway that is sensitive to inhibito

94 and demonstrates that observations made with Rps6 knock-ins must be interpreted cautiously.

95 Indeed, rpS6 knockin mice are completely sensitive to the inhibi

96 ss of SNpc neuron, nor did STN DBS elevate p-rpS6 levels further.

97 Here, we report that RPS6 may have a novel function via interaction with hist

98 additionally show that TOR function involves RPS6-mediated nutrition and light-dependent growth and l

99 The rpS6 mutants expressing oncogenic Kras showed increased

100 R1 also enhanced HopA1-triggered immunity in rps6 mutants.

101 - and loss-of-function Ribosomal Protein S6 (RPS6) mutants additionally show that TOR function involv

102 predicted secondary structure similarity to RPS6 of other eukaryotes.

103 d PKA exclusively phosphorylates recombinant rpS6 on Ser235/Ser236 in vitro.

104 nce that PKA is also likely to phosphorylate rpS6 on Ser235/Ser236 in vivo in a number of other mamma

105 similar effect is observed upon depletion of RPS6 or RPL11.

106 ts activated downstream targets p-RPS6KB1, p-RPS6, or p-EIF4EBP1 was associated with adverse clinical

107 Uremic rpS6(p-/-) mice had no increase in parathyroid cell prol

108 five phosphorylatable sites in rpS6 (termed rpS6(P-/-) mice).

109 Knockin rpS6(p-/-) mice, in which rpS6 cannot be phosphorylated

110 r DMBA or mutant Kras was greatly reduced in rpS6(P-/-) mice.

111 Here, we show that inhibition of the RPS6 pathway by rapamycin effectively suppressed, wherea

112 mTOR complex 1/ribosomal protein S6 (mTORC1/RPS6) pathway as well as the reduced expression of sever

113 These findings further our understanding of rpS6 phospho-regulation and define a direct link between

114 trate that Csnk1a1 inhibition causes reduced Rps6 phosphorylation and activation of p53, resulting in

115 of acinar ductal metaplasia, suggesting that rpS6 phosphorylation attenuates Kras-induced DNA damage

116 rscore the importance of mTOR activation and rpS6 phosphorylation for the pathogenesis of secondary h

117 with rpS6 phosphorylation, thus dissociating rpS6 phosphorylation from the translational control of t

118 3-acetate, known activators of PKC, leads to rpS6 phosphorylation in a rapamycin-dependent manner.

119 depicts the signaling cascades orchestrating Rps6 phosphorylation in budding yeast, challenges the no

120 me profiling, we failed to uncover a role of Rps6 phosphorylation in either global translation or tra

121 These results reveal that rpS6 phosphorylation is important for the initiation of

122 Furthermore, PMT-induced rpS6 phosphorylation is inhibited by PKC inhibitor, Go69

123 Surprisingly, we demonstrate that rpS6 phosphorylation is not required for any of these pr

124 cerevisiae, we found that the regulation of Rps6 phosphorylation on Ser-232 and Ser-233 is mediated

125 in budding yeast, challenges the notion that Rps6 phosphorylation plays a role in translation, and de

126 Heterogeneity in RPS6 phosphorylation was a consequence of the presence o

127 RpS6 phosphorylation was dramatically diminished within

128 as insulin-like growth factor I receptor and RPS6 phosphorylation were enriched in the "proliferation

129 genes as significant on-target regulators of RPS6 phosphorylation, and we characterized them with ext

130 that Csnk1a1 knockdown results in decreased Rps6 phosphorylation, increased p53 activity, and myeloi

131 ough regulatory mechanisms such as eIF4E and rpS6 phosphorylation, mediated by activation of the ERK1

132 us administration of IGF-1 rescued defective rpS6 phosphorylation, spine density, and PSD-95 expressi

133 oligopyrimidine tract, did not coincide with rpS6 phosphorylation, thus dissociating rpS6 phosphoryla

134 To examine the functional significance of rpS6 phosphorylation, we used knockin mice lacking all f

135 size, insulin levels, glucose tolerance, and RPS6 phosphorylation, without rescuing IUGR.

136 kinase(s) determine complex heterogeneity in RPS6 phosphorylation.

137 osmotic stress had no reproducible effect on RPS6 phosphorylation.

138 ion independently of its ability to regulate rpS6 phosphorylation.

139 tivation, it exerts no effect on PMT-induced rpS6 phosphorylation.

140 inal phospho-residues is required to sustain rpS6 phosphorylation.

141 aradoxical increase in ribosomal protein S6 (rpS6) phosphorylation and a decrease in eukaryotic initi

142 of MAPK signaling and the phosphorylation of rpS6 produced by D1 activation in D1-MSNs, paralleling b

143 this idea, CK1-dependent phosphorylation of rpS6 promotes its association with the mRNA cap-binding

144 Furthermore, a reduction in the level of RpS6 protein expression led to diminished expression fro

145 Genetic modulation of RPS6 protein levels with specifically targeted short hai

146 tracellular signal-regulated kinase) and S6K-RPS6 (ribosomal protein S6 kinase-ribosomal protein S6)

147 IL-5 or GM-CSF in maintaining the ERK/p90S6K/RPS6 ribosome-directed signaling pathway, leading to inc

148 0, RPS12, RPS18, RPL30, RPS20, RPL12, RPL7A, RPS6, RPL27A, RPLP2, RPS25, RPS3, RPL41, RPL6, RPLP0, RP

149 ction of intron-encoded nascent peptides and RPS6/RPL7-carrying complexes in the perinucleolar compar

150 ic Thr389 residue but not at Thr421/Ser424), rpS6 (Ser235/236) and 4E-BP1 (gel shift), as well as def

151 phorylation on Thr389 and phosphorylation of rpS6 (ser235/36), suggesting p70S6K kinase activity was

152 Immunoblotting for rpS6 showed modest polysomal disaggregation upon fasting

153 function, the deduced amino acid sequence of RPS6 shows highest similarity to the TNL resistance prot

154 Our screen revealed that the TORC1-S6K-RPS6 signaling axis is regulated by many subcellular com

155 hrough its role in the regulation of the RSK-rpS6 signaling module.

156 gh the alternative transcript structures are RPS6 specific.

157 s in RICTOR (25% of patients) and positive p-RPS6 staining correlated with recurrence.

158 the phosphorylation of ribosomal protein S6 (rpS6), suggesting activation of the ribosomal S6 kinases

159 ing mTORC1 signaling, whereas phosphorylated rpS6 suppresses cystogenesis and fibrosis in Tsc1-delete

160 e lacking all five phosphorylatable sites in rpS6 (termed rpS6(P-/-) mice).

161 ts with phosphorylated ribosomal protein S6 (RpS6) to promote its ubiquitylation and proteasomal degr

162 In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70

163 Maize RPS6 was analyzed by the use of two-dimensional gel elec

164 olysome fractions showed that phosphorylated rpS6 was disproportionately present in translating polys

165 Phosphorylation of rpS6 was increased in pancreatic acinar cells upon impla

166 Aberrant mTOR signaling (p-RPS6) was present in half of the cases, associated with

167 ins the key regulatory ribosomal protein S6 (rpS6), we considered that myc loss might affect expressi

168 The association of RpS6 with nsP2 was detected throughout the course of inf