ライフサイエンスコーパス: RRE

1 RRE recognition triggers a crucial coil-to-helix transit

2 and DNAs, and viral RNAs including the HIV-1 RRE and TAR.

3 The precise secondary structure of the HIV-1 RRE has been controversial, since studies have reported

4 reagents for selective eradication of HIV-1 RRE mRNA.

5 ent CRM1 pathway, which is used by the HIV-1 RRE.

6 The 3D models for the HIV-2 RRE and folding intermediates are also presented, wherei

7 ation, the secondary structures of the HIV-2 RRE and two RNA folding precursors have been identified

8 nalysis collectively suggests that the HIV-2 RRE undergoes two conformational transitions before assu

9 follow-up at 52 weeks (HIIT, 39%; MCT, 25%; RRE, 34%; P=0.16).

10 PE, we have now determined that the wt NL4-3 RRE exists as a mixture of both structures.

11 cence titration assay revealed high-affinity RRE RNA binding by all 22 metal-chelate-Rev species, wit

12 c compounds, as a specific and high-affinity RRE-IIB binder which inhibits the interaction of the Rev

13 ssess whether BALF5 might be activated by an RRE-dependent mechanism, an Rta mutant (Rta K156A), defi

14 gesting this promoter can be activated by an RRE-dependent mechanism.

15 stem by an additional base pair, creating an RRE that was more responsive to lower concentrations of

16 was observed with the constructs lacking an RRE.

17 t (Rta K156A), deficient for DNA binding and RRE activation but competent for Zp/Rp activation, was u

18 While the CTE and RRE primarily enhance nucleocytoplasmic export, PPE-like

19 Multiple Rev and RRE sequences were obtained using single-genome sequenci

20 Rev and RRE variants from each time point were subjected to func

21 protein expression in the absence of Rev and RRE.

22 We used a rev- and RRE-defective HIV type 1 (HIV-1) molecular clone in comp

23 able to promote replication of the Rev- and RRE-defective HIV-1 in both human lymphoid cell lines an

24 (nucleocapsid) locally melts the TAR RNA and RRE-IIB RNA hairpins, whereas arginine-rich motif protei

25 y, but formed a super complex with Sam68 and RRE in vitro.

26 ementary DNA oligonucleotides to the TAR and RRE-IIB RNA hairpins, respectively.

27 Rta binding sites are likely functioning as RREs.

28 ve slightly lower RRE affinities, but better RRE specificities.

29 In SSKH cells, Rev failed to activate both RRE-mediated reporter gene [chloramphenicol acetyltransf

30 s and sugar-phosphate backbones of the bound RRE.

31 embly and budding were blocked, but changing RRE to PRE rescued HIV-1 Gag assembly and budding.

32 also reflected at the levels of cytoplasmic RRE-chloramphenicol acetyltransferase mRNAs, indicating

33 REBP can modestly enhance Rev-dependent RRE-linked reporter gene expression both independently a

34 t the first crystal structure of a Rev dimer-RRE complex, revealing a dramatic rearrangement of the R

35 nter in a protein and suggest that dinuclear RRE species, not mononuclear DNICs, may be the primary i

36 l layer requires technologies for disrupting RREs without perturbing cellular homeostasis.

37 transactivation of reporters containing each RRE showed that their promoter strengths in a transient-

38 lectrophoretic mobility shift assays of each RRE demonstrated that the highly purified Rdbd protein d

39 ngth Ets-1 binds equivalently to BTE and EBS-RRE probes, while recombinant GABPalpha/beta preferentia

40 EMSAs, using either the BTE or EBS-RRE probes, identified a specific protein-DNA complex, d

41 osslinking experiments with radiolabeled EBS-RRE and BTE oligonucleotides showed that these probes sp

42 ning GABP, we were able to show that the EBS-RRE preferentially binds Ets-1, while the BTE binds both

43 hereas Ets-1 preferentially binds to the EBS-RRE.

44 Ss contained in the RRE and BTE, we used EBS-RRE and BTE oligonucleotides in electrophoretic mobility

45 he level of relative replication efficiency (RRE) depends on the number and type of transcription fac

46 RiPP precursor peptide recognition element (RRE).

47 The viral Rev response element (RRE) adopts an "A"-like structure in which the two legs

48 of virion (Rev) to the Rev response element (RRE) and subsequent oligomerization in a cooperative man

49 point mutation in the Rev response element (RRE) at the bottom of stem-loop IIC.

50 HIV-1 Rev and the Rev response element (RRE) enable a critical step in the viral replication cyc

51 The HIV-1 Rev response element (RRE) is a 351-base element in unspliced and partially sp

52 assembly of Rev on the Rev response element (RRE) is essential for the nuclear export of unspliced an

53 chelates to the HIV-1 Rev response element (RRE) mRNA have been synthesized.

54 multiple sites in the Rev response element (RRE) of viral mRNA transcripts in nuclei of host cells,

55 NA containing either a Rev response element (RRE) or a Mason-Pfizer monkey virus (MPMV) constitutive

56 V-1 protein Rev on the Rev Response Element (RRE) regulates nuclear export of genomic viral RNA and p

57 been proposed for the Rev-response element (RRE) responsible for viral mRNA export, how it recruits

58 oop II region of HIV-1 Rev response element (RRE) RNA enhanced binding of HIV-1 Rev protein to the RR

59 n homooligomer and the Rev response element (RRE) RNA to mediate nuclear export of unspliced viral mR

60 a homo-oligomer on the Rev response element (RRE) RNA.

61 al RNAs containing the Rev Response Element (RRE) through the Crm1 nuclear export pathway to the cyto

62 al Rev protein and the Rev response element (RRE), a structured element located in the Env region of

63 bunits assemble on the Rev Response Element (RRE), a structured region present in these RNAs, and dir

64 within viral RNA, the Rev response element (RRE), and escorts RRE-containing RNAs from the nucleus.

65 ral mRNAs encoding the Rev response element (RRE), thereby facilitating viral late gene expression.

66 virus (HIV) mRNAs, the Rev response element (RRE), to recruit the cellular nuclear export receptor Cr

67 transcripts harbor the Rev Response Element (RRE), which orchestrates the interaction with the Rev AR

68 or, unexpectedly, the Rev response element (RRE), which regulates the nuclear export of gRNAs and ot

69 P to mediate export of Rev response element (RRE)-containing human immunodeficiency virus (HIV) RNA,

70 zes with, HIV-1 Rev in Rev response element (RRE)-mediated gene expression and virus production.

71 ies with, HIV-1 Rev in Rev-response element (RRE)-mediated gene expression and virus replication.

72 v association with the Rev Response Element (RRE).

73 s, including the HIV-1 Rev response element (RRE).

74 V mRNAs containing the Rev response element (RRE).

75 d oligomerizing on the Rev Response Element (RRE).

76 hich we have named the Ras response element (RRE); whereas the response of multiple growth factors (F

77 ing frame 50 (ORF50)/Rta responsive element (RRE) and a TATA box.

78 cription activator (RTA) responsive element (RRE) are crucial cis-acting elements.

79 is dependent on the Ras responsive element (RRE) binding protein (RREB1), which negatively regulates

80 interactions with an RTA-responsive element (RRE) could complement the loss of one RBPjkappa binding

81 have identified the Rta-responsive element (RRE) in the PAN promoter.

82 interaction with the Rev responsive element (RRE) of HIV-1 mRNA has been studied by directly observin

83 viral elements, the Rev-responsive element (RRE) of the human immunodeficiency virus (HIV), and the

84 ly homologous to the RTA-responsive element (RRE) of the PAN promoter.

85 ions between the HIV Rev-responsive element (RRE) RNA and the HIV regulatory protein Rev, are crucial

86 ng its corresponding Rev Responsive Element (RRE) RNA aptamer.

87 munodeficiency virus Rev-responsive element (RRE) RNA by the Rev protein is an essential step in the

88 ind and activate the Rev-responsive element (RRE) RNA or heterologous MS2 phage operator RNA, we anal

89 the presence of the Rev responsive element (RRE) RNA to which it binds.

90 otein that binds the Rev responsive element (RRE) within the env gene of the HIV-1 RNA genome, activa

91 ously reported 16-bp RTA-responsive element (RRE), and the same mutation also both reduced RTA-mediat

92 As opposed to HIV's Rev-responsive element (RRE), the Rex-responsive element (RxRE) is present in al

93 SNV) facilitates Rev/Rev-responsive element (RRE)-independent expression of intron-containing human i

94 at also serves as an RTA-responsive element (RRE).

95 uclear export element (Rev-response element [RRE]) used by HIV-1 and EIAV with the hepatitis B virus

96 binding motifs and the R response elements (RRE) within oriLyt.

97 on by viral Rev and Rev-responsive elements (RRE).

98 s containing YUAAY RNA recognition elements (RREs).

99 RNA regulatory elements (RREs) are an important yet relatively under-explored fac

100 e RNA motifs known as Rev response elements (RREs) is required for transport of unspliced and partial

101 nate DNA sites termed Rta response elements (RREs).

102 a special focus on Ras responsive elements (RREs), the MAP kinases (Erks, p38 and JNK) and Ca2+-spec

103 sest relatives of Sam68, marginally enhanced RRE-mediated transactivation, while QK isoforms that are

104 c description using reaction rate equations (RREs) is no longer accurate.

105 the Rev response element (RRE), and escorts RRE-containing RNAs from the nucleus.

106 l model of a Rev dimer bound to an essential RRE hairpin and to visualize the complete Rev-RRE RNP, d

107 s)(2)(NO)(4)], having a Roussin's red ester (RRE) formula, and that mononuclear DNICs account for onl

108 structurally related to Roussin's Red Ester (RRE, [Fe2 (NO)4 (Cys)2 ]) and Roussin's Black Salt (RBS,

109 eities in tempo (relative rate of evolution, RRE) and mode (selection pressure, Ka/Ks) in six organis

110 M10 resistance, which prompted us to examine RRE structure using a novel chemical probing strategy.

111 CT, or a recommendation of regular exercise (RRE).

112 uction correlated with the failure to export RRE-containing CAT mRNA and unspliced viral mRNAs to the

113 To optimize these derivatives for RRE specificity, a series of neomycin-acridine conjugate

114 sed placement of the 2 legs is essential for RRE function.

115 e of these mutants with a null phenotype for RRE activated the heterologous MS2 RNA target.

116 e compromised their activation potential for RRE.

117 mutants that preserved the Rev response for RRE RNA localized to the nuclei; those with poor or no R

118 upport the essential role of the A shape for RRE function.

119 v binding and explains Rev's specificity for RRE-containing RNAs.

120 The presence of a functional RRE and a downstream TATA box suggested that this region

121 constants and chemical reactivity toward HIV RRE RNA have been determined and evaluated in terms of r

122 tions of fewer than eight arginines impaired RRE activation.

123 d nuclear residence times and differences in RRE binding affinity may have compromised their activati

124 not specific for the G-rich bubble region in RRE.

125 immunodeficiency virus type 1 (HIV-1) Rev in RRE (Rev response element)-mediated gene expression and

126 proteins still enhanced the effect of Rev in RRE-mediated gene expression.

127 r of Rev NES function and may play a role in RRE RNA transport during HIV infection.

128 d the functional contributions of individual RRE domains and now report that several domains contribu

129 analysis showed strong enrichment for known RREs but little or no enrichment for Rp or Zp, suggestin

130 sequencing (ChIP-seq) identified most known RREs and several novel Rta binding sites.

131 bramycin and kanamycin A have slightly lower RRE affinities, but better RRE specificities.

132 ghest RNA and DNA affinities, but the lowest RRE specificity.

133 Mean RRE of the 18 endocannabinoid genes was significantly gr

134 ibited Sam68-mediated, but not Rev-mediated, RRE-dependent gene expression.

135 fferent CpG-containing BRLF1 binding motifs (RREs) in vitro or in vivo.

136 loops from HIV-1 Rev Response Element mRNA (RRE RNA) and ribosomal 16S A-site RNA (16S RNA) by metal

137 footprinting results and studies with mutant RRE sequences indicate that the internal loop of RRE is

138 ing within the internal purine-rich bulge of RRE-IIB in a manner analogous to what has been observed

139 The rapid and sensitive detection of RRE-Rev interaction in complex biological systems repres

140 viral Rev protein induces nuclear export of RRE-containing RNAs, as required for virus replication.

141 stant, within the 67-nucleotide domain II of RRE.

142 al arginine-rich Rev peptide and stem IIB of RRE.

143 sequences indicate that the internal loop of RRE is required for specific binding of DB340 as with th

144 ional assay, we have now analyzed a panel of RRE mutants.

145 specific hydrogen bonding for recognition of RRE, shape recognition, through contact with the sugar-p

146 Titrations of RRE-IIB with proflavine, monitored using (1)H NMR, demon

147 in situ high-content functional analysis of RREs.

148 e the effects of these KH family proteins on RRE- and CTE (constitutive transport element of type-D r

149 t two classes of proflavine binding sites on RRE-IIB: a high-affinity site that competes with the Rev

150 s used to directly assess Rev "loading" onto RRE and its variants, indicating that this is unaffected

151 ration between them are required for optimal RRE function.

152 h the shortest linker length has the optimal RRE specificity.

153 turally intronless genes, but not the CTE or RRE from intron-containing genes, significantly enhanced

154 MCT at 60% to 70% of maximal heart rate, or RRE.

155 tures as closely related as the HIV-1 TAR or RRE elements.

156 me redundancy, to maintenance of the overall RRE shape.

157 ctly to the polyadenylated nuclear RNA (PAN) RRE motif, failed to bind to the RAP RRE and interfered

158 eptide's high-affinity mRNA binding partner, RRE stem loop IIB.

159 Selected patient RREs were also shown to have differences in Rev multimer

160 In all patients, RRE activity was more sensitive to sequence variation th

161 ory efficacy of proflavin on the Rev peptide-RRE binding, even in the presence of substantial levels

162 nsor for sensitively quantifying Rev peptide-RRE interaction and characterizing the potential inhibit

163 to what has been observed in the Rev peptide.RRE-IIB complex.

164 responsive element of the PAN promoter (pPAN RRE) was previously identified, and our data suggested d

165 ere mapped within a 16-bp region of the pPAN RRE by methylation interference assays.

166 , an extensive mutagenesis study on the pPAN RRE was carried out by using EMSAs and reporter assays.

167 ociation constant (K(d)) of Rdbd on the pPAN RRE was determined to be approximately 8 x 10(-9) M, sug

168 irect binding of full-length RTA to the pPAN RRE.

169 neity and measured its affinity for the pPAN RRE.

170 in the NOESY spectrum of the 2:1 proflavine.RRE-IIB complex indicate that the two proflavine molecul

171 ndicate that formation of the 2:1 proflavine.RRE-IIB complex stabilizes base pairing and stacking wit

172 ng interaction occurs with a 2:1 (proflavine:RRE-IIB) stoichiometry, and NOEs observed in the NOESY s

173 A (PAN) RRE motif, failed to bind to the RAP RRE and interfered with RRE-bound C/EBPalpha in EMSA exp

174 suggest that RTA transactivation of the RAP RRE is mediated by an interaction with DNA-bound C/EBPal

175 dithionite produces the one-electron-reduced RRE, having absorptions at 640 and 960 nm.

176 Rev-RRE assembly occurs via several Rev oligomerization and

177 agreement with previous models for HIV-1 Rev-RRE binding.

178 the full molecular structures of Rev and Rev-RRE complexes are not known.

179 RE hairpin and to visualize the complete Rev-RRE RNP, demonstrating that RRE binding drives assembly

180 and dissociation rates for the different Rev-RRE stoichiometries were determined.

181 Assembly of the functional Rev-RRE complex proceeds by cooperative oligomerization of R

182 tion is transferable and can replace HIV Rev-RRE-regulated expression of HIV gag.

183 trajectories recorded during individual Rev-RRE assembly reactions has revealed the microscopic rate

184 assembly and dissociation of individual Rev-RRE complexes in the presence or absence of DDX1 were ob

185 While a range of Rev-RRE activities were seen, the activity of cognate pairs

186 ich DDX1 promotes the nucleation step of Rev-RRE assembly.

187 rted in previous bulk kinetic studies of Rev-RRE association, indicating that oligomerization is an e

188 Replacement of Rev-RRE by the CTE provides a novel approach to dramatically

189 tering (SAXS) reveals two major steps of Rev-RRE complex formation, beginning with rapid Rev binding

190 in increased occurrence of higher-order Rev-RRE stoichiometries.

191 v monomer, thereby promoting the overall Rev-RRE assembly process.

192 or dominant-negative mutants suppressed Rev-RRE-function in the export of incompletely spliced HIV-1

193 Analysis of the Rev-RRE assembly pathway using SHAPE-Seq and small-angle X-r

194 ly accelerate the nucleation step of the Rev-RRE assembly process.

195 Formation of the Rev-RRE complex signals nucleocytoplasmic export of unsplice

196 Therefore, the Rev-RRE regulatory mechanism plays a key role in the mainten

197 el of activity throughout infection, the Rev-RRE system can fluctuate, presumably to control replicat

198 interface that enhances association with Rev-RRE and poises NES binding sites to interact with a Rev

199 d translational enhancement, SNV RU5 and Rev/RRE were combined on a single gag RNA.

200 antisense inhibition of HIV mediated by Rev/RRE.

201 CRM-1 is also used to export Rev/RRE-dependent unspliced/ partially spliced HIV-1 RNAs.

202 m of the RRE promotes greater functional Rev/RRE activity compared to the four stem-loop counterpart.

203 However, the relevance of Sam68 in Rev/RRE function is not well defined.

204 Instead, Rev/RRE diverts RU5 gag RNA to the CRM1-dependent, LMB-inhib

205 -1 structural proteins in the absence of Rev/RRE is caused by inefficient accumulation of mRNA in the

206 eport that like snRNAs and snoRNAs, some Rev/RRE-dependent HIV-1 RNAs are TMG-capped.

207 zymatic activities in the context of the Rev/RRE pathway.

208 ignificantly alter the efficiency of the Rev/RRE pathway.

209 ficking of the antisense RNA through the Rev/RRE pathway.

210 iochemical activities with regard to the Rev/RRE system, while DDX3 differs.

211 Mechanistic studies indicated that the Rev/RRE-mediated inhibition did not involve either nuclear r

212 uality of CRM1's interactions with viral Rev/RRE ribonucleoprotein complexes in the nucleus.

213 ) into a truncated form of the RRE sequence (RRE-IIB) allowed the binding of an arginine-rich peptide

214 uorescently labeled Rev monomers to a single RRE molecule was visualized, and the event frequencies a

215 at both proteins can associate with a single RRE molecule.

216 Rev and its RNA binding site RRE can be replaced in both human immunodeficiency virus

217 The sequence-specific RRE RNA-Rev binding is essential for HIV-1 replication a

218 are necessary and sufficient for substantial RRE function, provided they are joined by a flexible lin

219 dependent viral gag-pol mRNAs bearing tandem RREs (GP-2xRRE), rescue virus particle production in mur

220 two RREs, thereby plausibly targeting tandem RREs present in many QKI-targeted transcripts.

221 their high-affinity binding to the targeted RRE mRNA following coupling to the Rev peptide, this cla

222 the complete Rev-RRE RNP, demonstrating that RRE binding drives assembly of Rev homooligomers into as

223 The RRE assembles a Rev oligomer that displays nuclear expor

224 eins that can bind to HIV RNA containing the RRE in vivo but are unable to mediate the export of this

225 accounts for the specificity of Rev for the RRE and thus the specific recognition of the viral RNA.

226 340, exhibits pronounced selectivity for the RRE RNA stem-loop from HIV-1.

227 , can explain the specificity of Rev for the RRE.

228 ew compounds have a high specificity for the RRE.

229 the activation for MS2 RNA, but not for the RRE.

230 However, two silent G->A mutations in the RRE (RRE61) confer RevM10 resistance, which prompted us

231 H3NE) that bind to the EBSs contained in the RRE and BTE, we used EBS-RRE and BTE oligonucleotides in

232 unds to various nucleic acids, including the RRE, tRNA, and duplex DNA.

233 a high-affinity site in stem-loop IIB of the RRE and proceeds rapidly by addition of single Rev monom

234 stem, we demonstrated that the region of the RRE and TATA box constitutes an ORF50/Rta-dependent prom

235 partially spliced viral RNA; binding of the RRE by the viral Rev protein induces nuclear export of R

236 onstrate that the five stem-loop form of the RRE promotes greater functional Rev/RRE activity compare

237 nopurine (2-AP) into a truncated form of the RRE sequence (RRE-IIB) allowed the binding of an arginin

238 hin the three-way junction of stem II of the RRE that is responsible for initial Rev binding.

239 e high-affinity stem-loop IIB segment of the RRE.

240 igh affinity for the Rev binding site on the RRE (K(d) <or= 10 nM), but few compounds have a high spe

241 that is otherwise unable to assemble on the RRE beyond a monomeric complex.

242 by cooperative oligomerization of Rev on the RRE scaffold and utilizes both protein-protein and prote

243 in enhancement of Rev oligomerization on the RRE that is correlated with an RNA structural change wit

244 cate that Rev assembles cooperatively on the RRE via a series of symmetrical tail-to-tail and head-to

245 uating the inhibitory effect of drugs on the RRE-Rev interaction.

246 r by promoting oligomerization of Rev on the RRE.

247 d by cooperative Rev-Rev interactions on the RRE.

248 model for Rev and its multimerization on the RRE.

249 at mapped to the envelope region outside the RRE.

250 DX1 acts as an RNA chaperone, remodeling the RRE into a conformation that is pre-organized to bind th

251 y to assemble onto its HIV-1 RNA target (the RRE) as a multimeric complex.

252 uggest that DDX1 targets Rev rather than the RRE to promote oligomeric assembly.

253 We previously reported that the RRE assumes an "A" shape in solution and suggested that

254 Here we show that the RRE controls the oligomeric state and solubility of Rev

255 We previously reported that the RRE is "A" shaped and suggested that this architecture,

256 Deletion analysis revealed that the RRE is present in a region between nucleotides -69 and -

257 mutants, we show that DDX1 acts through the RRE RNA to specifically accelerate the nucleation step o

258 Thus, the RRE is not simply a passive scaffold onto which proteins

259 ry Ets factors that specifically bind to the RRE and BTE sites remains unknown.

260 DX21 was shown to enhance Rev binding to the RRE in a manner similar to that previously described for

261 enhanced binding of HIV-1 Rev protein to the RRE in vivo and influenced nuclear export of RNA.

262 nity binding of multiple Rev monomers to the RRE is achieved on a much faster timescale than reported

263 A expression by direct binding of Rta to the RRE of the PAN promoter.

264 ggest that RSG 1.2 binds more tightly to the RRE sequence than Rev by forming more base-specific cont

265 show here that initial binding of Rev to the RRE triggers RNA tertiary structural changes, enabling f

266 t the binding of an ORF50/Rta protein to the RRE was essential for ori-Lyt-dependent DNA replication.

267 We found that DDX21 can bind to the RRE with high affinity, and this binding stimulates ATPa

268 Furthermore, when the RRE-driven antisense RNA was redirected to the Tap/Nxf1

269 The structure supports a model in which the RRE utilizes the inherent plasticity of Rev subunit inte

270 ssay, both K-Rta and ORF59 interact with the RRE and C/EBPalpha binding motifs within oriLyt in cells

271 ripts in vivo, interacting directly with the RRE and Rev in vitro, and promoting Rev oligomerization

272 Rta formed a highly stable complex with the RRE of the PAN promoter.

273 yt DNA through RTA, which interacts with the RRE, as well as K8, which binds to a cluster of C/EBP bi

274 HIV-1 virus production was obtained with the RRE-driven antisense RNA.

275 ted with an RNA structural change within the RRE that persists even after dissociation of DDX1.

276 We undertook a comparative study of the RREs of PAN RNA, ORF57, vIL-6, and Kpsn to understand ho

277 purified Rdbd protein directly bound to the RREs.

278 nsfections magnifies the difference in their RREs.

279 assess functional differences between these RRE 'conformers', we created conformationally locked mut

280 Reduction of this RRE reaction product with sodium dithionite produces the

281 inds with better specificity and affinity to RRE than the Rev peptide.

282 HnRNP K did not bind to RRE-RNA directly, but formed a super complex with Sam68

283 the stoichiometry of Rev peptide binding to RRE can be accurately determined by using this single-QD

284 competes with the Rev peptide for binding to RRE-IIB (K(D) approximately 0.1 +/- 0.05 microM) and a w

285 proflavine competes with Rev for binding to RRE-IIB by binding as a dimer to a single high-affinity

286 The results indicate that DB340 binds to RRE in a highly structured and cooperative complex at a

287 essential viral protein Rev, which binds to RRE RNA, to export their unspliced and partially spliced

288 de-RNA complexes of Rev and RSG 1.2 bound to RRE stem IIB have been solved and reveal gross structura

289 e parameter identifiability in comparison to RRE.

290 ed and cooperative complex at a 2:1 DB340 to RRE ratio.

291 n uptake (P=0.70), but both were superior to RRE.

292 d that enables concurrent recognition of two RREs, thereby plausibly targeting tandem RREs present in

293 43/145 promoter through interaction with two RREs.

294 e N-terminal domain of SuiB adopts a typical RRE (RiPP recognition element) motif, which has been imp

295 ing replication, identify previously unknown RREs, such as one in BALF5p, and highlight the complexit

296 dramatic rearrangement of the Rev-dimer upon RRE binding through re-packing of its hydrophobic protei

297 end-diastolic diameter changes compared with RRE were -2.8 mm (-5.2 to -0.4 mm; P=0.02) in HIIT and -

298 the Rev and RSG 1.2 peptides in complex with RRE stem IIB have been simulated to better understand on

299 d to bind to the RAP RRE and interfered with RRE-bound C/EBPalpha in EMSA experiments.

300 bits the interaction of the Rev peptide with RRE-IIB.