ライフサイエンスコーパス: RRV

1 RRV and EW but not UK NSP1 was proteasomally degraded, r

2 RRV gene segment 4 plays a significant role in governing

3 RRV infectivity is also reduced by inhibitors of clathri

4 RRV is the closest relative to KSHV that has a fully seq

5 RRV naturally infects rhesus macaques and induces lympho

6 RRV particles are also colocalized with transferrin, whi

7 RRV replication was significantly rescued in IFN types I

8 RRV T48 carrying the six nonsynonymous DC5692 nucleotide

9 RRV trafficking was reduced by an inhibitor of the dynei

10 RRV VP4 reduced murine RV infectivity only slightly; how

11 RRV-infected hOBs produced high levels of inflammatory c

12 RRV-T48-nsP1(6M) loads in skeletal muscle tissue, but no

13 RRV-TV in a 2-dose schedule with the first dose during t

14 RRVs have a convincing preference for replicating in tum

15 HC tetramer staining in the two Mamu-A*01(+) RRV-negative monkeys reached 9.3% and 13.1% of all CD8(+

16 We have constructed a RRV recombinant virus (RRVDeltaLANA/GFP) in which the RR

17 Stat1-/- mice with rhesus rotavirus type A (RRV) on postnatal day 1 induced a prominent Th2 response

18 sia, a characteristic pathology during acute RRV infection that often develops into more severe lymph

19 D8(+) T cells contribute to control of acute RRV infection.

20 We found that acute RRV infection induces activation of CD8(+) T cell respon

21 In addition, RRV(prop) was a predictor of sugar intake but not of tot

22 Gene segment 3 did not affect RRV infectivity in vitro but altered its in vivo effect.

23 latory T cells (Tregs) at baseline and after RRV infection compared to WT mice.

24 1 and Stat6 in mice did not prevent BA after RRV infection.

25 aggravated bile duct injury at 12 dpi after RRV inoculation, as plasma bilirubin levels were elevate

26 0 expression limits immune responses against RRV at early times postinfection and also impacts viral

27 ent of protective antibody responses against RRV.

28 Ig-alpha(-/-) RRV-infected mice had significantly increased disease-fr

29 able BAC clone, we successfully generated an RRV mutant with a deletion of Orf50, which encodes a rep

30 lineate the immunomodulatory mechanism of an RRV vIRF and its ability to assist the virus in rapid im

31 4)), where gene 4 from TUCH was placed on an RRV background, eliminated the ability of RRV to cause m

32 "gain-of-function" reassortants in which an RRV gene replaced its TUCH equivalent were generated.

33 HHV-8 and RRV encode homologues of CD200, termed vCD200, which are

34 Both HHV-8 and RRV encode viral CD200 (vCD200) molecules that are homol

35 ctivity between an anti-enolase antibody and RRV proteins indicates that molecular mimicry might acti

36 ues and prevented dissemination of CHIKV and RRV at early time points.

37 ments suggest that IRF-1 restricts CHIKV and RRV infection in stromal cells, especially muscle cells,

38 se antibodies cross-reacted with enolase and RRV proteins; we identified regions of sequence homology

39 rine NIH 3T3 cells in contrast to the EW and RRV NSP1 proteins.

40 lear antigen (LANA) protein of both KSHV and RRV plays key roles in the establishment and maintenance

41 MneRV2 and RRV belong to the rhadinovirus 2 (RV2) rhadinovirus line

42 espite the attenuated phenotype, RRV T48 and RRV-T48-nsP1(6M) loads in tissues of wild-type and Rag1(

43 n agreement with these findings, RRV T48 and RRV-T48-nsP1(6M) loads were similar in mice deficient in

44 Genetic reassortant analysis between UK and RRV maps the distinctive phenotypes of IFN antagonism an

45 Using reassortants between UK and RRV, we previously demonstrated that the differential re

46 Anti-RRV and anti-enolase antibodies cross-reacted with enola

47 y cytokines and earlier induction of an anti-RRV T cell response compared to wild-type RRV infection.

48 s, and despite the appearance of strong anti-RRV antibody responses in immunized monkeys, anti-Env an

49 I IFN, was also inhibited following WT(BAC) RRV infection.

50 osome clone of wild-type RRV(17577) (WT(BAC) RRV) to generate a recombinant virus with all 8 of the v

51 o RRV than in cultures infected with WT(BAC) RRV.

52 ntified regions of sequence homology between RRV and enolase.

53 igates the basis for the interaction between RRV and human host cells (tumor versus nontumor) in vitr

54 atment with PPS reduced the severity of both RRV- and CHIKV-induced musculoskeletal disease, includin

55 eplication phenotype of RRV was conferred by RRV VP4 and NSP1.

56 Here, we demonstrate a strategy employed by RRV to ensure rapid inhibition of virus-induced type I I

57 lasts (hOBs) can be productively infected by RRV.

58 Inhibition of IFN induction by RRVs and the reduced response to IFN should facilitate t

59 Using a chimeric RRV NSP1 protein containing the carboxyl 100 residues de

60 In contrast, RRV-T48 E2 Y18H replicated more efficiently than RRV-T48

61 hat the ability of CD8(+) T cells to control RRV infection is tissue dependent.

62 uggest that the type I IFN response controls RRV infection in a tissue-specific manner and that speci

63 ke KSHV, which grows poorly in cell culture, RRV replicates efficiently in rhesus fibroblasts (RFs).

64 nonsynonymous DC5692 nucleotide differences (RRV-T48-nsP1(6M)) was attenuated in both wild-type and R

65 tracked gp33-specific CD8(+) T cells during RRV-lymphocytic choriomeningitis virus infection.

66 induction of type I and type II IFNs during RRV infection of peripheral blood mononuclear cells.

67 We now report that ORF52 knockdown during RRV infection of rhesus fibroblasts led to a greater tha

68 f which was alleviated by PPS therapy during RRV and CHIKV clinical disease.

69 These data suggest that early RRV trafficking is confined to the early endosome compar

70 mice depleted of CD8(+) T cells had elevated RRV loads in skeletal muscle tissue, but not joint-assoc

71 with RRV was validated using an established RRV murine model.

72 This study examined RRV-TV for the prevention of rotavirus gastroenteritis (

73 In agreement with these findings, RRV T48 and RRV-T48-nsP1(6M) loads were similar in mice

74 Consistent with these findings, RRV-T48 E2 Y18H replicated less well in mammalian cells

75 ue damage than wild-type (WT) mice following RRV infection.

76 The relative reinforcing value of food (RRV(food)) is associated with obesity and energy intake

77 dicity for BFV, intra-annual periodicity for RRV, and trend for dengue.

78 es and microtubule dynamics are required for RRV trafficking to perinuclear regions.

79 Compared with those for RRV, R(T(VP4)) binding and titers in cholangiocytes were

80 ions in single reassortment groups, and four RRV mutants contained mutations in multiple groups.

81 ture with hepatic dendritic cells (DCs) from RRV-infected, but not with DCs from noninfected mice, wh

82 significantly contributed to protection from RRV-induced mortality, and both mouse strains exhibited

83 Furthermore, RRV particles are colocalized with microtubules and dyne

84 ep replication kinetics of RRVDeltaLANA/GFP, RRV-GFP, wild-type (WT) RRV H26-95, and a revertant viru

85 How RRV(food) is related to macronutrient choice in ad libit

86 d blocking assays, this study elucidated how RRV VP4 protein governs cholangiocyte susceptibility to

87 nificantly reduced symptoms and mortality in RRV-injected mice.

88 amage to musculoskeletal tissues observed in RRV- or CHIKV-infected mice would promote a wound-healin

89 The perturbed RANKL/OPG ratio in RRV-infected OBs may therefore contribute to bone loss i

90 However, exogenous type I IFN inhibited RRV replication in tumor cells and induced IFN-regulated

91 magnitude in the monkeys that were initially RRV negative but were still readily detected in the two

92 assortants in which a TUCH gene replaced its RRV equivalent and 11 single-gene "gain-of-function" rea

93 (vIRF) deletion clone of RRV (vIRF-knockout RRV [vIRF-ko RRV]) demonstrated that vIRFs inhibit induc

94 Experimental infection of RMs with vIRF-ko RRV resulted in decreased viral loads and diminished B c

95 blood mononuclear cells (PBMCs) with vIRF-ko RRV resulted in earlier and increased induction of type

96 Moreover, in vivo infection with vIRF-ko RRV resulted in earlier and sustained production of proi

97 ction in PBMC cultures infected with vIRF-ko RRV than in cultures infected with WT(BAC) RRV.

98 rus with all 8 of the vIRFs deleted (vIRF-ko RRV).

99 ion clone of RRV (vIRF-knockout RRV [vIRF-ko RRV]) demonstrated that vIRFs inhibit induction of type

100 Moreover, RRVs appear to carry a heat-labile component that active

101 Three NIH single mutant RRV viruses, RRVtsD(7), RRVtsJ(5), and RRVtsK(2), were i

102 We demonstrated that the RRV mutant (RRV(VP4-R446G)) produced less symptomatology and replica

103 a production in pDCs, suggesting that native RRV has a dominant inhibitory effect on type I IFN induc

104 sis at early times during infection, neither RRV nor SB1A effectively inhibited the activation of Y70

105 ese findings demonstrate that during de novo RRV infection, vIRFs are inhibiting the induction of IFN

106 RVSRLY) significantly reduced the ability of RRV to bind and infect cells.

107 an RRV background, eliminated the ability of RRV to cause murine BA.

108 tion of a viral IRF (vIRF) deletion clone of RRV (vIRF-knockout RRV [vIRF-ko RRV]) demonstrated that

109 thesis is supported by the colocalization of RRV antigens with the early endosome markers Rab4 and Ra

110 Thus, to address the contributions of RRV vCD200 to immune regulation and disease in vivo, we

111 nstrate a role for T cells in the control of RRV infection and suggest that the antiviral capacity of

112 hat T cells can contribute to the control of RRV infection in the absence of B cells and Ab.

113 ino acid changes in nsP1 are determinants of RRV virulence by regulating the sensitivity of RRV to in

114 n strains T48 and DC5692 are determinants of RRV virulence, and we identify two nonsynonymous nucleot

115 we recruited neonates to receive 2 doses of RRV-TV or placebo and followed them to age 12 months.

116 f clathrin-coated pits, blocked the entry of RRV into RFs.

117 ratio was disrupted in the synovial fluid of RRV patients, and this was accompanied by an increase in

118 and disease in vivo, we generated a form of RRV that lacked expression of vCD200 for use in infectio

119 analyzed the properties of a mutant form of RRV that lacks vCD200 expression in infected rhesus maca

120 Though the functions of RRV and HHV-8 vCD200 molecules have been examined in vit

121 er the aberrant codon usage for gH and gL of RRV is an intentional regulatory strategy used by the vi

122 n vivo infection in RMs, the natural host of RRV.

123 luciferase), we show that the infectivity of RRV is reduced by inhibitors of endosomal acidification.

124 ectors and conditions yielded high levels of RRV gB expression.

125 Furthermore, while high levels of RRV-specific antibody were produced in TLR7-deficient mi

126 protein ORF52 is critical for maturation of RRV, the closest relative of Kaposi's sarcoma-associated

127 Using a mouse model of RRV-induced myositis/arthritis, we found that myeloid di

128 nuated disease phenotype in a mouse model of RRV-induced rheumatic disease.

129 Mouse models of RRV and chikungunya virus have demonstrated a role for t

130 Mouse models of RRV and CHIKV disease were used to characterize the exte

131 everse genetics system to create a mutant of RRV (RRV(VP4-R446G)) with a single amino acid change in

132 , we have characterized the entry pathway of RRV in RFs.

133 4 and that the high-replication phenotype of RRV was conferred by RRV VP4 and NSP1.

134 The attenuated phenotype of RRV-T48 E2 Y18H was associated with reduced viral loads

135 t E2 position 18 functions as a regulator of RRV fitness in vertebrate and invertebrate cells.

136 We found that systemic replication of RRV, but not murine rotavirus strain EC, was greatly enh

137 V virulence by regulating the sensitivity of RRV to interferon.

138 tations in nsP1 influence the sensitivity of RRV to type I interferon only in specific host tissues.

139 ynein-dynactin complexes in the transport of RRV particles to nuclei during primary infection.

140 RRV nsP1 gene that control the virulence of RRV and its sensitivity to the antiviral type I interfer

141 Coincubation of RRVs with heat-treated RRVs or with lentivirus vector su

142 lation of the costimulatory molecule CD86 on RRV-primed DCs.

143 ic regression models to surveillance data on RRV, BFV, and dengue (from 1993, 1995 and 1991, respecti

144 The tripeptide SRL on RRV VP4 binds to the cholangiocyte membrane protein Hsc7

145 One RRV construct expressed nonstructural protein 5 (NS5), w

146 IRF being associated with either the KSHV or RRV virion.

147 the number of calcium-channels determined P(RRV), it critically influenced whether subsequent releas

148 by regulating the RRV release probability (P(RRV)) and the RRV number.

149 th (release of 0, 1 or multiple vesicles), P(RRV), short-term plasticity, calcium transients and the

150 Despite the attenuated phenotype, RRV T48 and RRV-T48-nsP1(6M) loads in tissues of wild-ty

151 n musculoskeletal tissues at late times post-RRV infection.

152 f responding for food compared with reading (RRV(prop)) was positively related to body mass index, la

153 cell-deficient (Ig-alpha(-/-)) mice received RRV shortly after birth.

154 these questions, we generated a recombinant RRV expressing the H-2(b)-restricted glycoprotein 33 (gp

155 Using a luciferase-expressing recombinant RRV (RRV-luciferase), we show that the infectivity of RR

156 rescent protein (RFP)-expressing recombinant RRV (RRV-RFP), we show that RRV particles are colocalize

157 ccination with a mixture of both recombinant RRVs and were subsequently challenged 19 weeks later wit

158 ly with a mixture of these three recombinant RRVs.

159 n of Hsc70 by small interfering RNAs reduced RRV's ability to infect cholangiocytes.

160 transferred T cells were capable of reducing RRV loads in skeletal muscle tissue of Rag1(-/-) mice, i

161 sely related to rhesus macaque rhadinovirus (RRV) and human herpesvirus 8.

162 closely related rhesus macaque rhadinovirus (RRV) are the only viruses known to encode viral homologu

163 Rhesus macaque rhadinovirus (RRV) is a gammaherpesvirus of rhesus macaque (RM) monkey

164 Rhesus macaque rhadinovirus (RRV) is the RV2 prototype, and two RRV isolates, 26-95 a

165 irus (KSHV) and rhesus macaque rhadinovirus (RRV), are unique in that they express viral homologues t

166 herpesvirus and rhesus macaque rhadinovirus (RRV), two closely related gammaherpesviruses, are unique

167 primate virus, rhesus macaque rhadinovirus (RRV), which infects and induces disease in rhesus macaqu

168 ruses, including rhesus monkey rhadinovirus (RRV) and its close homolog, the oncogenic human gammaher

169 ther recombinant rhesus monkey rhadinovirus (RRV) could be used as a vaccine against DENV2 infection

170 Rhesus monkey rhadinovirus (RRV) is a gammaherpesvirus that is closely related to hu

171 cation-competent rhesus monkey rhadinovirus (RRV) were constructed in which strong promoter/enhancer

172 ly reported that rhesus monkey rhadinovirus (RRV), a close homolog of the human pathogen Kaposi's sar

173 of gH and gL of rhesus monkey rhadinovirus (RRV), a close relative of the human Kaposi's sarcoma-ass

174 In rhesus monkey rhadinovirus (RRV), a close relative of the human oncogenic pathogen K

175 cation-competent rhesus monkey rhadinovirus (RRV), a persisting herpesvirus.

176 Rhesus rhadinovirus (RRV) is a gammaherpesvirus closely related to Kaposi's s

177 role of microtubules in rhesus rhadinovirus (RRV) nuclear trafficking in rhesus fibroblasts.

178 ed an infectious BAC of rhesus rhadinovirus (RRV) strain RRV26-95 with the BAC vector cassette insert

179 iled macaque homolog of rhesus rhadinovirus (RRV).

180 erpesvirus rhesus macaque (RM) rhadinovirus (RRV) are the only known viruses to encode viral homologu

181 e-sensitive (ts) mutants and seven rotavirus RRV ts mutants, isolated at the National Institutes of H

182 y perinatal infection with rhesus rotavirus (RRV) but not with other strains of rotavirus, such as TU

183 Rhesus rotavirus (RRV) can also lead to biliary atresia (a neonatal human

184 In neonatal mice, rhesus rotavirus (RRV) can induce biliary atresia (BA), a disease resultin

185 ction of newborn mice with rhesus rotavirus (RRV) results in biliary atresia (BA), and this condition

186 strain) but not the simian rhesus rotavirus (RRV) robustly triggers beta interferon (IFN-beta) secret

187 ine model of BA, employing rhesus rotavirus (RRV), parallels human disease and has been used to eluci

188 yte injury in human and in rhesus rotavirus (RRV)-induced experimental biliary atresia (BA).

189 biliary obstruction in the Rhesus rotavirus (RRV)-induced mouse model of BA.

190 by serum antibodies in the Rhesus rotavirus (RRV)-induced mouse model of BA; findings were correlated

191 nt expression of Gal1-R in Rhesus rotavirus (RRV)-infected mice and its contribution to fluid secreti

192 old greater than that of a simian rotavirus (RRV) in suckling mice.

193 rologous simian rotavirus (rhesus rotavirus [RRV]) efficiently degrade cellular IRF3, diminish IRF3 a

194 e genetics system to create a mutant of RRV (RRV(VP4-R446G)) with a single amino acid change in the V

195 ing a luciferase-expressing recombinant RRV (RRV-luciferase), we show that the infectivity of RRV is

196 nt protein (RFP)-expressing recombinant RRV (RRV-RFP), we show that RRV particles are colocalized wit

197 age input virus by monitoring the rhesus RV (RRV) antigens VP4, VP6, and VP7 at very early times post

198 Rab5 but not Rab4 or Rab7 affects rhesus RV (RRV) infectivity.

199 naling is involved in protection from severe RRV-associated disease.

200 Furthermore, we reveal that the severe RRV-induced joint pathology, including thinning of artic

201 ne UK strain but not the mouse EW and simian RRV strains.

202 d-type SA11 or a reassortant encoding simian RRV NSP1.

203 /-) mice revealed that murine but not simian RRV mediated accumulation of IkB-alpha protein and decre

204 Simian (RRV) and bovine (UK) rotaviruses have distinctive replic

205 Infection of human HT29 IECs with simian (RRV) or porcine (SB1A or OSU) RV strains, which inhibit

206 sensitive to innate antiviral effectors than RRV T48 in a tissue-specific manner.

207 T48 E2 Y18H replicated more efficiently than RRV-T48 in C6/36 mosquito cells.

208 Competition studies confirmed that RRV-T48 E2 Y18H had a fitness advantage in mosquito cell

209 We furthermore demonstrate that RRV enters the cell cytoplasm through an endocytosis pat

210 In addition, we also demonstrate that RRV infection affects CD200R expression levels in vivo,

211 In the current study, we found that RRV infection activated the extracellular signal-regulat

212 Using confocal imaging, we found that RRV infection led to the thickening and acetylation of M

213 We found that RRV vIRF R6, when expressed ectopically, interacts with

214 Indeed, we found that RRV- and CHIKV-induced musculoskeletal inflammatory lesi

215 As a result, we hypothesized that RRV might induce changes in the cytoskeleton at both ear

216 Together, these results indicate that RRV entry into RFs is mediated by clathrin-mediated endo

217 Our results indicate that RRV gH and gL expression is severely limited by the stab

218 Our data indicated that RRV vCD200 expression limits immune responses against RR

219 aveola-mediated endocytosis, indicating that RRV enters into RFs via clathrin-mediated endocytosis.

220 Our findings show that RRV infection damages the articular cartilage, including

221 These data show that RRV infection of the small intestine increases colonic s

222 sing recombinant RRV (RRV-RFP), we show that RRV particles are colocalized with markers of endocytosi

223 In summary, our data provide evidence that RRVs do not directly trigger type I IFN responses in IFN

224 We found that RRVs do not trigger an IFN-alpha/beta response in tumor

225 Surprisingly, the data show that RRVs can actively inhibit induction of cellular innate i

226 a 511 and human host cells, and we show that RRVs do not induce type I interferon (IFN) responses in

227 The RRV-infected Ig-alpha(-/-) mice had significantly less l

228 the RRV release probability (P(RRV)) and the RRV number.

229 he mechanisms behind immunomodulation by the RRV vIRFs during infection.

230 s study, we identified coding changes in the RRV nsP1 gene that control the virulence of RRV and its

231 ence of this immunomodulatory protein in the RRV virion provides the virus with an immediate mechanis

232 trate that a single amino acid change in the RRV VP4 gene influences cholangiocyte tropism and reduce

233 sequence SRL (amino acids 445 to 447) in the RRV VP4 protein is required for viral binding and entry

234 re protected from biliary obstruction in the RRV-induced mouse model of BA, indicating a primary role

235 s toward the incorporation of pERK2 into the RRV particle.

236 8 of the E2 glycoprotein (E2 Y18H), into the RRV-T48 genetic background was sufficient to generate a

237 rk contrast to the RRV-infected BA mice, the RRV-infected Ig-alpha(-/-) mice did not have hyperbiliru

238 Among the NIH mutants, three of the RRV mutants and all four SA11 mutants contained mutation

239 These data support the utility of the RRV-/SIV-infected RM as an excellent animal model to inv

240 ing a 2-ms depolarization) by regulating the RRV release probability (P(RRV)) and the RRV number.

241 We demonstrated that the RRV mutant (RRV(VP4-R446G)) produced less symptomatology

242 dues derived from UK NSP1, we found that the RRV NSP1 carboxyl 100 residues are critical for its IRF3

243 We previously reported that the RRV VP4 gene plays an integral role in activating the im

244 type and Rag1(-/-) mice, suggesting that the RRV-T48-nsP1(6M) mutant is more sensitive to innate anti

245 In stark contrast to the RRV-infected BA mice, the RRV-infected Ig-alpha(-/-) mic

246 is responsible for pathogenesis, we used the RRV and TUCH strains to generate a complete set of singl

247 binant virus (RRVDeltaLANA/GFP) in which the RRV LANA open reading frame has been disrupted with a gr

248 Three RRV-negative and two RRV-positive rhesus monkeys were in

249 her lytic replicative properties compared to RRV-GFP, WT RRV, or the revertant virus.

250 at all time points postinfection compared to RRV-GFP.

251 with the RRVDeltaLANA/GFP virus compared to RRV-GFP.

252 tion and Th1 cytokine production compared to RRV-infected WT mice.

253 but not of CD25-depleted CD4 cells, prior to RRV inoculation reduced expansion of CD8 cells, plasma b

254 , whereas lentivirus vector and heat-treated RRVs did.

255 Coincubation of RRVs with heat-treated RRVs or with lentivirus vector suppressed IFN-alpha prod

256 inovirus (RRV) is the RV2 prototype, and two RRV isolates, 26-95 and 17577, were sequenced.

257 Three RRV-negative and two RRV-positive rhesus monkeys were inoculated intravenousl

258 ti-RRV T cell response compared to wild-type RRV infection.

259 ial artificial chromosome clone of wild-type RRV(17577) (WT(BAC) RRV) to generate a recombinant virus

260 and in vitro than those seen with wild-type RRV, with reduced binding in cholangiocytes.

261 he VP4 protein compared to that of wild-type RRV.

262 BAC-excised virus was reversed to wild-type RRV.

263 Unexpectedly, RRVs did not induce IFN-alpha production upon incubation

264 tetravalent, rhesus-based rotavirus vaccine (RRV-TV), led to the withdrawal of the vaccine.

265 n reassortant rotavirus tetravalent vaccine (RRV-TV) has been studied extensively on the basis of Cen

266 n reassortant rotavirus tetravalent vaccine (RRV-TV) was licensed in 1998 but withdrawn in 1999 due t

267 s (MLV)-based retroviral replicating vector (RRV), Toca 511, which has displayed tumor specificity in

268 1-10 (mean, 5) readily releasable vesicles (RRVs) and released 0-5 vesicles during a 2-ms depolariza

269 protein 1 (nsP1) gene of the mouse-virulent RRV strain T48 with that from the mouse-avirulent strain

270 genic alphaviruses such as Ross River virus (RRV) and chikungunya virus (CHIKV) cause large-scale epi

271 kungunya virus (CHIKV) and Ross River virus (RRV) cause a debilitating, and often chronic, musculoske

272 Ross River virus (RRV) is one of a group of mosquito-transmitted alphaviru

273 kungunya virus (CHIKV) and Ross River virus (RRV), and assessed the early antiviral functions of IRF-

274 Ross River virus (RRV), Barmah Forest virus (BFV), and dengue are three co

275 Ross River virus (RRV), chikungunya virus, and related alphaviruses cause

276 ic alphaviruses, including Ross River virus (RRV), infect humans and cause debilitating pain and infl

277 kungunya virus (CHIKV) and Ross River virus (RRV), pose significant public health threats because of

278 nic alphaviruses including Ross River virus (RRV), Sindbis virus, and chikungunya virus cause worldwi

279 egion of the T48 strain of Ross River virus (RRV-T48) with that from the attenuated DC5692 strain, wh

280 ly 1 million cases reported to date, whereas RRV continues to circulate in the South Pacific.

281 To determine which RRV gene segment(s) is responsible for pathogenesis, we

282 ased risk of intussusception associated with RRV-TV at all ages studied.

283 ased risk of intussusception associated with RRV-TV for the exposure window 3-14 days after the first

284 Infection of bone cells with RRV was validated using an established RRV murine model.

285 structure, and evolutionary comparisons with RRV and KSHV have identified conserved promoters, splice

286 hat this virus shares a number of genes with RRV that may be involved in pathogenesis but also indica

287 wo monkeys that were naturally infected with RRV at the time of immunization.

288 nolase in a mouse model of BA (infected with RRV) and in serum samples from patients, indicating a ro

289 tio was also disrupted in mice infected with RRV; both this effect and the bone loss were blocked by

290 We found that infection with RRV, a homolog of the human pathogen KSHV, led to perinu

291 /6J mice (wild type or Gal1-R knockout) with RRV or vehicle, closed small intestinal and colon loops

292 Reassortants with RRV VP4 and UK NSP1 genes induced high levels of express

293 Myd88- and TLR7-deficient mouse strains with RRV revealed that both Myd88 and TLR7 significantly cont

294 Viruses with RRV VP4 entered cultured mouse cholangiocytes more effic

295 In addition, we found R6 within RRV virion particles via immunoelectron microscopy and,

296 plicative properties compared to RRV-GFP, WT RRV, or the revertant virus.

297 ed disease-free survival rate compared to WT RRV-infected BA mice (76.8% vs. 17.5%).

298 of RRVDeltaLANA/GFP, RRV-GFP, wild-type (WT) RRV H26-95, and a revertant virus using traditional plaq

299 Intestinal replication of a series of EW x RRV reassortants was used to identify several RV genes t

300 rus infection of mouse gallbladder with UK x RRV reassortants to study the genetic and mechanistic ba