ライフサイエンスコーパス: RT-PCR

1 RT-PCR allowed a specific and accurate amplification of

2 RT-PCR analysis of 17 human tissues demonstrated ubiquit

3 RT-PCR analysis of CEP78 in blood leukocytes of affected

4 RT-PCR analysis revealed dramatic up-regulation of the g

5 RT-PCR analysis showed strong upregulation of OsPIP1;3 a

6 RT-PCR and immunostaining experiments indicated that HAT

7 RT-PCR and in situ hybridization revealed CPAMD8 express

8 RT-PCR and qRT-PCR were performed to confirm our finding

9 RT-PCR based expression analysis of seven glucosinolate

10 RT-PCR confirmed positive synergistic hypoxia/IL-15 inte

11 RT-PCR data also showed that the expression of mature mi

12 RT-PCR demonstrated that 10 microM YC-1 reduced hypoxia-

13 RT-PCR is the preferred testing method; providers should

14 RT-PCR of mature miR-17-92 in cells demonstrated the sel

15 RT-PCR on whole kidney RNA and serum profiling indicated

16 RT-PCR products for AMPK-alpha1 and -alpha2 isoforms and

17 RT-PCR provided the highest number of positive detection

18 RT-PCR revealed that transcripts for the five activating

19 RT-PCR sequencing of 16 childhood ependymoma samples ide

20 RT-PCR, protein immunoblots, and in vitro plasmablast di

21 Sequencing of approximately 50,000 RT-PCR products spanning the exon 7-8 junction in 10 tis

22 On admission, RT-PCR analysis of blood specimens from patients who die

23 using a mouse anti-Zika virus antibody, and RT-PCR assays targeting the NS5 and envelope genes.

24 Western Blot and RT-PCR demonstrated downregulation of CDK1 and CDK4 and

25 Western blot and RT-PCR were performed to quantitate Hsp90 pathway expres

26 The MRM UHPLC-MS/MS method, Western blot and RT-PCR were used along with SULT activity measurement us

27 oducts, were analyzed using conventional and RT-PCR to determine the presence of pork DNA.

28 4 by immunofluorescence, flow cytometry, and RT-PCR.

29 Using FACS and RT-PCR, we examined the phenotype of generated IgE(+) ce

30 drome, n = 3) using immunohistochemistry and RT-PCR and compared them with specimens from healthy con

31 and angiogenesis by immunohistochemistry and RT-PCR.

32 Immunohistological and RT-PCR analysis of muscle biopsy specimens from anti-MDA

33 By confocal microscopy and RT-PCR expression of Rac1/CdC42 Rho GTPases, responsible

34 In addition, the RT-RPA and RT-PCR assays do not cross-react with dengue and chikung

35 tronic RNAs via RNA sequencing (RNA-seq) and RT-PCR.

36 oratory testing including mumps serology and RT-PCR.

37 RT-PCR) assays; an accurate sample-to-answer RT-PCR test would reduce time to results and potentially

38 odimeric proteins, we used an emulsion-based RT-PCR assay to link and amplify TCR pairs.

39 Both RT-PCR analysis and crossing into Rosa26-lacZ reporter m

40 RNA than CXCR5(-) subsets, as determined by RT PCR.

41 nd non-matched control samples (p < 0.01) by RT-PCR and immunostaining.

42 ACS), and V(D)J transcripts are amplified by RT-PCR.

43 LNs from 256 patients were analyzed by RT-PCR; 176 (69%) were PCR-positive (52% in N1 position,

44 ve for dengue virus infection as assessed by RT-PCR.

45 (3)H-thymidine incorporation and TGF-beta by RT-PCR and ELISA.

46 nfected patients, 25 (21%) were confirmed by RT-PCR of serum collected 1-8 days after the onset of si

47 ysis whereas the expression was confirmed by RT-PCR, Western blot and enzyme activity.

48 of individual transporters was confirmed by RT-PCR.

49 alpha7 nAChR mRNA is detected by RT-PCR and cell surface expression of alpha7 nAChR is de

50 cruzi deoxyribonucleic acid was detected by RT-PCR at 30, 60, 90, 120, 150, 180, and 360 days.

51 lected, A. aegypti yielded ZIKV detection by RT-PCR in 15 of 55 pools (27.3%).

52 Arbovirus RNA detection by RT-PCR should be part of the management of GBS cases.

53 IDO1 expression was determined by RT-PCR, Western blot, and FACS, and enzymatic activity b

54 The evidence of ZIKV infection documented by RT-PCR among patients with the Guillain-Barre syndrome d

55 mRNA and local trafficking were examined by RT-PCR and a retention-release methodology.

56 ransiently transfected cells was examined by RT-PCR, western blot analysis, and immunohistochemistry.

57 on of the following transcription factors by RT-PCR: RUNX2, osterix, and the osteoblast protein, oste

58 h chromatin immunoprecipitation, followed by RT-PCR analysis, we demonstrate that endogenous Osr2 pro

59 nd connexin (Cx) isoforms were identified by RT-PCR.

60 100% reduction in expression as measured by RT-PCR, Western blotting, or Luminex technology.

61 Gene expression analyses were performed by RT-PCR.

62 ogy; for HMPV, 172 (8.5%) tested positive by RT-PCR and 147 (7.3%) by serology; for the PIVs, 94 (4.6

63 r RSV, 287 (14.2%) patients were positive by RT-PCR and 234 (11.6%) were positive by serology; for HM

64 ; and for AdV, 111 (5.5%) tested positive by RT-PCR and 62 (3.1%) by serology.

65 ; for the PIVs, 94 (4.6%) tested positive by RT-PCR and 92 (4.6%) by serology; and for AdV, 111 (5.5%

66 Patients positive by RT-PCR were randomly assigned to observation versus CLND

67 st GSH, Grx1 and Trx1 levels as reflected by RT-PCR, Western blotting and immunohistochemistry analys

68 ve cases were positive for Zika virus RNA by RT-PCR, and sequence analyses showed highest identities

69 ing HBGAs through detection of viral RNAs by RT-PCR and capsid antigens by immunostaining methods.

70 ogy but with melanoma detected in the SLN by RT-PCR, there was no OS benefit for CLND or CLND+HDI.

71 C. jejuni and C. coli genomes, supported by RT-PCR testing, indicated that the assay was robust, wit

72 M positive, and 27 of the 40 (68%) tested by RT-PCR were positive.

73 y in Sierra Leone for routine EVD testing by RT-PCR ("Trombley assay").

74 We validated by RT-PCR and Sanger sequencing, the predicted splice isofo

75 sible primers for experimental validation by RT-PCR and displays those, along with the matching prote

76 patients who had samples tested for ZIKV by RT-PCR, the results were positive in 17 patients (40%).

77 logy, molecular pharmacology and single cell RT-PCR in mice.

78 acterized isolated cells using a single-cell RT-PCR strategy for gene expression analysis and flow cy

79 dorsal root ganglion neurons by single-cell RT-PCR.

80 Using molecular cloning, RT-PCR, Western blotting, immunolocalization and in vitr

81 Current RT-PCR assays lack sensitive and reliable positive contr

82 nction was observed by Fluidigm high-density RT-PCR between responders and nonresponders.

83 erse transcription (RT) and obtain different RT-PCR patterns for an ssRNA knot and circle of the same

84 it and the derived RealStar Zaire Ebolavirus RT-PCR kit were validated using in vitro transcripts, su

85 Of 5,126 patients enrolled, RT-PCR and serology test results were available for 2,02

86 llent performance compared to an established RT-PCR benchmark on WB and BS samples in a field laborat

87 ence of bleeding, and 5.2-fold higher if EVD RT-PCR cycle threshold value was </=20.

88 Finally, RT-PCR and Western blot results revealed a significant r

89 itivity similar to the one already found for RT-PCR, NASBA, and RT-LAMP assays.

90 Of 158 sera/plasma from RT-PCR-confirmed ZIKV infections, 145 (91.8%) yielded gr

91 Well-characterized samples from RT-PCR-confirmed patients with Zika and individuals expo

92 subjects; 5637 (94%) were analyzed; 18% had RT-PCR-confirmed A(H1N1)pdm09-related MAARI.

93 ole combined with benznidazole achieved high RT-PCR conversion rates during treatment (30 days; 93.3%

94 erapy is superior to posaconazole, with high RT-PCR conversion rates sustained at 1 year.

95 in situ hybridization, immunohistochemistry, RT-PCR, northern blot and western blot experiments.

96 d a bio-inspired positive control for use in RT-PCR diagnostics: we encapsulated scrambled Ebola RNA

97 Virological investigations included RT-PCR for Zika virus, and both microsphere immunofluore

98 Moreover, RT-PCR analysis confirmed that the expression of the RNA

99 nasopharyngeal DNA quantitative PCR and mRNA RT-PCR should be used for accurate diagnosis of HBoV1 in

100 In contrast, mRNA RT-PCR had low clinical sensitivity (75%) but high speci

101 The multiplex RT-PCR assay showed 95% sensitivity and 100% specificity

102 portion of subjects with persistent negative RT-PCR by day 180; the secondary outcome was negative RT

103 day 180; the secondary outcome was negative RT-PCR at 360 days.

104 Consistent with this notion, RT-PCR of lymphocyte cell lines from one of the kindreds

105 nfluenza infection was confirmed by means of RT-PCR assay and culture of nasopharyngeal swabs obtaine

106 All specimens were tested by means of RT-PCR, and serum was tested with the use of anti-ZIKV I

107 A panel of RT-PCR assays was used to detect noninfluenza respirator

108 ctors as long as 12 d, but the proportion of RT-PCR positive whiteflies dropped to 55% by 3 d.

109 essfully acquire CCYV, and the proportion of RT-PCR positive whitefly individuals reached to 100% at

110 Results of RT-PCR and sequencing were confirmed by staining cell cu

111 limited sensitivity, the detection window of RT-PCR for Zika viremia is only about one week after sym

112 with 1484 (12%) of these cases confirmed on RT-PCR assay.

113 t signal produced from probe-based RT-RPA or RT-PCR assays can be monitored using LEDs and a smartpho

114 ymerase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been propos

115 mic data to evaluate a duplex real-time PCR (RT-PCR) assay that targets mapA and ceuE for the detecti

116 A quantitative real-time PCR (RT-PCR) method, employing novel primer sets designed on

117 ing both conventional PCR and real-time PCR (RT-PCR).

118 ne-step multiplex reverse transcriptase PCR (RT-PCR) to simultaneously detect, quantify, and differen

119 ti-step real-time reverse transcription PCR (RT-PCR) assays; an accurate sample-to-answer RT-PCR test

120 itive Ebola virus reverse transcription PCR (RT-PCR) test, age >/= 1 y, weight >/= 10 kg, ability to

121 Finally, reverse transcription PCR (RT-PCR)-based screening for two specific RNA bacteriopha

122 ompared real-time reverse transcription-PCR (RT-PCR) and serology for the diagnosis of respiratory sy

123 sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the rapid detection of TiLV in fi

124 ed with real-time reverse transcription-PCR (RT-PCR) assays targeting the ZKV envelope and NS2B genes

125 uencing and novel reverse transcription-PCR (RT-PCR) assays to distinguish all RNA species in the KSH

126 loped a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A measles virus (Me

127 FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies the most critical genomic

128 encing (RNA-Seq), reverse transcription-PCR (RT-PCR), Western blot, and secretome analyses establishe

129 o confirmed using reverse transcription-PCR (RT-PCR).

130 s by quantitative reverse transcription-PCR (RT-PCR).

131 Unlike conventional PCR, RT-PCR detected pork DNA in nine processed food samples

132 ta of human high-grade gliomas and performed RT-PCR on glioblastoma sphere-forming cell lines (GSC).

133 A positive RT-PCR in semen was found in ten (5%) of 188 men, at a m

134 ion of Zika virus-specific IgM or a positive RT-PCR result in neonates.

135 Most of the positive RT-PCR results were in urine samples (in 16 of the 17 pa

136 ples (in 16 of the 17 patients with positive RT-PCR results), although 3 samples of cerebrospinal flu

137 After validating candidate miRNAs by q-RT-PCR, we selected miR-193a-3p for further investigatio

138 ative real-time polymerase chain reaction (Q-RT-PCR).

139 Quantitative RT-PCR analysis has been performed to determine if the I

140 Quantitative RT-PCR analysis revealed that TASK mRNA was reduced by >

141 Quantitative RT-PCR and ELISA were used to determine transcript and s

142 Quantitative RT-PCR and immunohistochemistry analysis identified that

143 Quantitative RT-PCR and microRNA (miRNA) array analysis revealed an i

144 Quantitative RT-PCR and western blot analyses indicated that both Pie

145 Quantitative RT-PCR assays using apical tissues showed that GA biosyn

146 Quantitative RT-PCR for miRNA analysis was performed using 70 serum s

147 Quantitative RT-PCR revealed significant inverse associations between

148 Quantitative RT-PCR was used to investigate mRNA expression in coloni

149 Quantitative RT-PCR, immunohistochemistry and immunoblots were carrie

150 Microarray analysis, quantitative RT-PCR, and immunoblotting revealed that IRF2 regulated

151 se chain reaction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proinflammatory cytokines inte

152 = 10 patients; 90 samples) and quantitative RT-PCR (validation set; n = 71 patients, 517 samples).

153 RNA sequencing and quantitative RT-PCR analyses identified reduced transcript levels dur

154 ng bisulfite pyrosequencing and quantitative RT-PCR in monocytes in vitro differentiated to macrophag

155 microscopy, histochemistry and quantitative RT-PCR methods.

156 staining of Ki67 and TUNEL and quantitative RT-PCR to determine the expression of PCNA and Bax/Bcl-2

157 was evaluated by classical and quantitative RT-PCR, and proteins were detected by Western blot analy

158 e exclusion chromatography, and quantitative RT-PCR, we provide the first characterization of a high

159 iocyanate-dextran uptake assay, quantitative RT-PCR analysis of tight junction proteins, myosin light

160 Y11, and P2Y13 was confirmed by quantitative RT-PCR and immunocytochemistry.

161 nscripts were later analysed by quantitative RT-PCR in age-matched sedentary rats.

162 ants bearing these mutations by quantitative RT-PCR revealed a competition relationship between these

163 GLL) expression was analyzed by quantitative RT-PCR, Western blot, and immunohistochemistry.

164 veral of which were verified by quantitative RT-PCR.

165 ts were further investigated by quantitative RT-PCR.

166 l lung tissue was determined by quantitative RT-PCR.

167 Zika virus-specific IgM and by quantitative RT-PCR.

168 of Fan1 expression confirmed by quantitative RT-PCR.

169 BER genes were assessed by quantitative RT-PCR.

170 dization, immunohistochemistry, quantitative RT-PCR, and functional analyses of cultured Schwann cell

171 istology, immunohistochemistry, quantitative RT-PCR, ELISA, and flow cytometry.

172 xis, apoptosis, ELISA, Luminex, quantitative RT-PCR, and flow cytometric assays.

173 Using cDNA microarray, quantitative RT-PCR, and immunohistochemistry, we molecularly charact

174 Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified a candidate

175 xtraction of RNA, the multiplex quantitative RT-PCR assay provides rapid diagnosis for the differenti

176 RNA sequencing and multiplexed quantitative RT-PCR have become more accessible, and these technologi

177 7 (27%) had positive results on quantitative RT-PCR.

178 ononuclear cells and performing quantitative RT-PCR, GIMAP6 was found to be expressed in CD3+ cells.

179 ted in independent LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin b

180 zed by means of RNA sequencing, quantitative RT-PCR, flow cytometry, and functional assays.

181 ry, electron microscopy, siRNA, quantitative RT-PCR, Western blot, and proteome analysis for the inve

182 able to the multiplex real-time quantitative RT-PCR (qRT-PCR) assay for DENV-1, -3, and -4 detection

183 transcription assays, real-time quantitative RT-PCR (RT-qPCR), and flow cytometry.

184 For real-time quantitative RT-PCR analysis of p15, RNA was extracted from FFPE sect

185 Real-time quantitative RT-PCR analysis revealed a lower mean qDeltaCt value in

186 Real time quantitative RT-PCR and 2-dimensional-PAGE showed that gamma-tubulin-

187 Msx2 gene silencing, real-time quantitative RT-PCR data showed significant overexpression of Runx2,

188 Real-time quantitative RT-PCR profiling led to the identification of miRNAs tha

189 RNAs was confirmed by real-time quantitative RT-PCR validation.

190 1), were confirmed by real-time quantitative RT-PCR, and DNA methylation of their promoter regions wa

191 ution flow cytometry, real-time quantitative RT-PCR, and mass spectrometry were used to characterize

192 ction, we have used an unbiased quantitative RT-PCR screening approach to identify MSC-derived peptid

193 y genes was determined by using quantitative RT-PCR (qRT-PCR) in cell pellets.

194 Using quantitative RT-PCR analyses (validation set), the key components hap

195 n levels were assessed by using quantitative RT-PCR and ELISA and a multiplex kit for IL-35 and IL-12

196 By using quantitative RT-PCR, we confirmed morphine-induced alterations in MMP

197 Using quantitative RT-PCR, we detected matriptase mRNA in several regions o

198 examined mtRNA expression using quantitative RT-PCR.

199 expression was quantified using quantitative RT-PCR.

200 xpression was measured by using quantitative RT-PCR.

201 ormal-weight children) by using quantitative RT-PCR.

202 ort of lung tumor tissues using quantitative RT-PCR.

203 tion of Zika virus genomes with quantitative RT-PCR and for detection of IgM antibodies with capture-

204 and protein were detected with quantitative RT-PCR and Western blotting, respectively, in both plate

205 ction (RT-PCR) for CMV DNA with quantitative RT-PCR performed on positive specimens.

206 zing RNA-seq data combined with quantitative RT-PCR validation, we found that a subset of alternative

207 rse-transcription polymerase chain reaction (RT-PCR) analysis in 17 cases and by serology in 6 cases.

208 rse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry, PCFT transcripts and p

209 rse-transcription polymerase chain reaction (RT-PCR) and in situ hybridization to test if GPR30 is ex

210 rse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting, confirming a concentration

211 rse transcription polymerase chain reaction (RT-PCR) experiments showed that TGFBR1 and TGFBR2 expres

212 rse transcriptase polymerase chain reaction (RT-PCR) for carcinoembryonic antigen.

213 itative real-time polymerase chain reaction (RT-PCR) for CMV DNA with quantitative RT-PCR performed o

214 rse-transcription polymerase chain reaction (RT-PCR) for laboratory-confirmed influenza virus infecti

215 ured by real time polymerase chain reaction (RT-PCR) in asymptomatic Chagas carriers.

216 rse transcription-polymerase chain reaction (RT-PCR) kit and a malaria rapid diagnostic test, respect

217 rse transcription-polymerase chain reaction (RT-PCR) kit and the derived RealStar Zaire Ebolavirus RT

218 rse transcription-polymerase chain reaction (RT-PCR) kits are available commercially, but validity da

219 rse-transcription polymerase chain reaction (RT-PCR) testing was not performed, resulting in a missed

220 rse transcription-polymerase chain reaction (RT-PCR) to analyse the effects of these treatments on nu

221 rse transcription polymerase chain reaction (RT-PCR) ZIKV test using highly sophisticated instruments

222 rse-transcription polymerase chain reaction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proi

223 rse transcription polymerase chain reaction (RT-PCR), for detection of DENV serotypes 1-4, and enzyme

224 rse-transcription polymerase chain reaction (RT-PCR), play an important role in the recent Zika outbr

225 rs with real-time polymerase chain reaction (RT-PCR)-confirmed EVD were enrolled retrospectively in 5

226 rse transcription-polymerase chain reaction (RT-PCR).

227 rse-transcription polymerase chain reaction (RT-PCR).

228 rse transcriptase polymerase chain reaction (RT-PCR).

229 itative real-time polymerase chain reaction (RT-PCR)and in situ hybridization assays, we validated th

230 rse-transcriptase-polymerase-chain-reaction (RT-PCR) assay in urine or blood in an enhanced arboviral

231 rse-transcriptase-polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP

232 rse-transcriptase-polymerase-chain-reaction (RT-PCR) assay, with consistent findings on electron micr

233 rse-transcriptase-polymerase-chain-reaction (RT-PCR) assay.

234 rse-transcriptase-polymerase-chain-reaction (RT-PCR) assays for ZIKV in blood, cerebrospinal fluid, a

235 rse-transcriptase polymerase-chain-reaction (RT-PCR)-confirmed, protocol-defined, influenza-like illn

236 Using a high-resolution RT-PCR panel, we found that the sr45-1 mutation broadly

237 ions and overexpression by exome sequencing, RT-PCR, and Western blotting.

238 tified by RT-qPCR, RNA gel blot, and in situ RT-PCR analyses.

239 sion of these TE-lincRNAs by strand-specific RT-PCR and also demonstrated tissue-specific transcripti

240 pes by means of immunofluorescence staining, RT-PCR, and qRT-PCR, and qRT-PCR analysis revealed incre

241 on to levels undetectable under our standard RT-PCR conditions.

242 The RT-PCR analysis of PRDM13 expression in developing retin

243 The RT-PCR analysis of selected genes was performed in stem

244 Among RIV4 recipients, the RT-PCR-confirmed influenza attack rate was 2.2% (96 case

245 This RT-PCR method showed greater sensitivity and reliability

246 Real-time RT-PCR also showed dramatically increased expression of

247 approaches, including microarray, real-time RT-PCR and Illumina sequencing are capable of detecting

248 Using real-time RT-PCR and immunoblotting analyses, we measured mRNA exp

249 ring 1-3 days after illness onset, real-time RT-PCR and NS1 antigen testing detected 82%-69% and 90%-

250 were tested for Ebola virus RNA by real-time RT-PCR and participants received counselling on safe sex

251 Real-time RT-PCR and sequencing of the VP1-2A region (1100bp) was

252 nd infectious EBOV was detected by real-time RT-PCR and virus culture out to 290 days and 70 days, re

253 nockdown of COX-2 was confirmed by real-time RT-PCR and Western blot analyses.

254 ipts and proteins were detected by real-time RT-PCR and western blots, respectively.

255 correlated well with those of the real-time RT-PCR assays used as benchmarks.

256 Quantitative real-time RT-PCR confirmed the circadian regulation of FLORE, wher

257 Real-time RT-PCR data showed that over-expression of the Notch Int

258 testing and virus culture but not real-time RT-PCR identified the end of the infectious period.

259 In comparison to the standard real-time RT-PCR method, the MeVA RT-qPCR showed 99.5% specificity

260 ors had enrolled in the programme; real-time RT-PCR results were available from 429 participants.

261 Real-time RT-PCR showed no relation to the cessation of transmissi

262 and probe nucleotide sequences for real-time RT-PCR testing.

263 of detection of Ebola virus RNA by real-time RT-PCR varies by individual and might be associated with

264 was quantified using quantitative real-time RT-PCR with bacterial group-specific primers.

265 Quantitative real-time RT-PCR, ELISA, and immunohistochemistry revealed that el

266 Using real-time RT-PCR, immunoblotting and immunostaining analyses, we m

267 Ebola virus RNA using quantitative real-time RT-PCR.

268 , spleen and brain by quantitative real-time RT-PCR.

269 tion) were checked by quantitative real-time RT-PCR.

270 Serology can be a helpful adjunct to RT-PCR for research-based assessment of the etiologic co

271 in reaction (PCR) and reverse-transcription (RT) PCR were applied to nasopharyngeal swab (NPS) sample

272 current gold standard reverse transcription (RT)-PCR approach with 100% concordance.

273 termined by real-time reverse transcription (RT)-PCR confirmed the presence of mEV71 in the sera and

274 , with mRNA detection based principally upon RT-PCR assays.

275 We used RT-PCR to detect latency-associated transcripts and HSV-

276 We used RT-PCR to profile ionotropic glutamate receptor expressi

277 Using RT-PCR and immunohistochemistry, we show TXNRD2 and ATXN

278 Using RT-PCR from blood, we examined expressed MHC class I all

279 Using RT-PCR targeting the L-polymerase gene of the Paramyxovi

280 outbreaks to detect CV-A6 and CV-A10, using RT-PCR.

281 ry and Western blotting and ex vivo by using RT-PCR after laser microdissection.

282 , and tissue biomarkers (determined by using RT-PCR and immunohistochemistry) were evaluated.

283 mean SCORAD score, 39) were studied by using RT-PCR, gene arrays, immunohistochemistry, and immunoflu

284 ed for rhinovirus and other viruses by using RT-PCR.

285 LISA, and expression of transcripts by using RT-PCR.

286 itute an operon, which we corroborated using RT-PCR.

287 mined viral persistence in body fluids using RT-PCR.

288 of IL-17A upon tenocytes were measured using RT-PCR, multiplex cytokine assays, apoptotic proteomic p

289 ion of genes of interest were measured using RT-PCR, proteomic array and ELISA.

290 IL-12/23p40 and IL-23p19 were measured using RT-PCR.

291 Next, using RT-PCR we measured the expression levels of these three

292 were amplified, cloned, and sequenced using RT-PCR.

293 Independent validation using RT-PCR revealed that DNA methylation inversely correlate

294 specimens for presence of Ebola virus using RT-PCR.

295 ' sp. SDB genome scaffold, were detected via RT-PCR, implying that paraffins are activated via 'fumar

296 cing of platelets followed by validation via RT-PCR and immunoblot, we discovered that granzyme A (Gr

297 Semi-quantitative Ebola virus RT-PCR (results expressed in "cycle threshold" [Ct]) and

298 Here we report Ebola virus RT-PCR data for body site and fluid samples from a large

299 The primary end point was RT-PCR-confirmed, protocol defined, influenza-like illne

300 aboratory-confirmed influenza diagnosed with RT-PCR.