ライフサイエンスコーパス: Rho GEF

1 Rho GEFs in animals fall into two structurally distinct

2 There are over 80 Rho GEFs in the human genome (compared to only 22 genes

3 eptin complex and activates Rho4 GTPase as a Rho GEF for septation in fission yeast.

4 These results for the first time define a Rho GEF involved in vascular smooth muscle cell growth a

5 neurabin revealed an interaction with Lfc, a Rho GEF.

6 Reduced function of Pebble, a Rho GEF required for cytokinesis, also delayed and slowe

7 eleton and provide the first evidence that a Rho-GEF transduces signals between G protein-coupled rec

8 Additionally, the AKAP13 Rho-GEF and PKD-binding domains mediate cardiomyocyte hy

9 ted mutagenesis, phosphorylation of alphaPIX Rho-GEF serines 225 and 488 is required for activation o

10 tandem DH-PH arrangement is conserved among Rho GEFs, the presence of the CRD is unique to Vav famil

11 The family is unique among Rho GEFs, as their activity is regulated by the synergis

12 activity of UNC-73, a homolog of the Rac and Rho GEF Trio.

13 , as well as a redesign of existing RhoA and Rho GEF activity assays so that they work in nuclear sam

14 l can be also used for other Rho GTPases and Rho GEFs, which have also been found in the nucleus.

15 s synergistically activated by Galpha(q) and Rho GEFs.

16 activities of the nuclear pools of RhoA and Rho GEFs.

17 uggest that interactions between septins and Rho-GEFs provide a new targeting mechanism for GTPases i

18 eotide exchange factor (LARG), also known as Rho GEF 12 (ARHGEF12) acts downstream of clustered ICAM-

19 luding PDZ-RhoGEF (PRG), leukemia-associated Rho GEF (LARG), p115-RhoGEF (p115), lymphoid blast crisi

20 for Rho, PDZ-RhoGEF, and Leukemia-associated Rho GEF (LARG).

21 eotide exchange factors, leukemia-associated Rho GEF (LARG; GEF, guanine nucleotide exchange factors)

22 which we named LARG for leukemia-associated Rho GEF.

23 ependent on Fus2p, an amphiphysin-associated Rho-GEF homolog.

24 Our data suggest the interaction between Rho-GEFs and anillins is an important step in the signal

25 oned full-length XLfc, a microtubule-binding Rho-GEF.

26 Type 2 nodes containing Blt1p, Rho-GEF Gef2p, and kinesin Klp8p remain intact throughou

27 action of LARG and related G-protein-coupled Rho GEFs with RhoA without a detectable effect on other

28 e oncogenic Rho-guanine nucleotide exchange (Rho-GEF) protein Lbc, and a unique region capable of bin

29 tive Rho-guanine nucleotide exchange factor (Rho-GEF) domain.

30 Rho-type guanine nucleotide exchange factor (Rho-GEF) homologous to Beta-PIX and Alpha-PIX in mammals

31 the Rho guanine nucleotide exchange factors (Rho GEFs) activate Rac GTPases to regulate cell migratio

32 Rho guanine nucleotide exchange factors (Rho GEFs) and Rho GTPases are among the key regulators o

33 Rho guanine nucleotide exchange factors (Rho GEFs) transduce extracellular signals to Rho, and we

34 family guanine nucleotide exchange factors (Rho-GEFs) regulate a variety of processes involving cell

35 cular characterization of a novel Dbl family Rho GEF, GEF64C, that promotes axon attraction to the ce

36 hout a detectable effect on other DBL family Rho GEFs, Rho effectors, or a RhoGAP.

37 We focused on Dbl family Rho GEFs, which have a highly modular structure common t

38 retinoids induce the expression of the FERM Rho-GEF protein FARP1 in the developing spinal cord.

39 ast two-hybrid to identify obscurin, a giant Rho-GEF protein, as the major cytoplasmic ligand for sAn

40 However, no studies have yet implicated Rho-GEFs as molecular regulators of the mesenchymal cell

41 t not of ECT2, a centralspindlin-interacting Rho GEF.

42 anlagen expresses Daam1 and its interacting Rho-GEF (WGEF), which compose one PCP/noncanonical Wnt p

43 regulate LMC dendritic growth, and that its Rho-GEF domain is necessary for this function.

44 SMC led to increased membrane-associated Lbc Rho GEF, suggesting modulation by 5-HT.

45 nd serotonin signaling, and suggest that Lbc Rho GEF family members play distinct roles.

46 in-protein interaction with G protein-linked Rho GEFs, thus providing a novel potential mechanism for

47 Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved

48 Recently another group of mammalian Rho-GEFs was discovered that includes CDM (Ced-5, DOCK18

49 ce potentially misleading results since many Rho GEFs can interact with multiple Rho proteins promisc

50 Multiple Rho GEFs have been proposed to link Galpha12/13 GPCRs to

51 c42 and Rac1 and constitute a novel class of Rho GEFs.

52 homology to several members of the family of Rho GEFs that includes such oncogenes as Dbl, Vav, Tiam,

53 wo-hybrid screens, we identified a family of Rho GEFs, named RopGEFs.

54 adigm for regulating local concentrations of Rho-GEFs.

55 However, the regulation of Rho-GEFs themselves is not well understood.

56 short hairpin RNA-based functional screen of Rho-GEFs for their roles in leukocyte chemotaxis and ide

57 ant RhoAL63, it does not affect Galpha12- or Rho GEF-induced RhoA activation or RhoAL63-GTP binding i

58 ure, including nuclear isolation and RhoA or Rho GEF activity assay, takes 1 h 40 min.

59 Like other Rho GEFs, XPLN contains a tandem Dbl homology and plecks

60 ected with dominant negative mutants of p115-Rho GEF or RhoA, and by inhibitors of Rho kinase (ROCK).

61 The combination of the putative Rho GEF and two kinase domains has not been noted in any

62 Here we show that putative Rho-GEF Gef2 and Polo kinase Plo1 coordinate to control

63 We previously found that putative Rho-GEF Gef2 coordinates with Polo kinase Plo1 to contro

64 mple of a general mechanism whereby receptor/Rho GEF pairings play an important role in receptor tyro

65 DH-PH domain of LARG, a G-protein-regulated Rho GEF involved in RhoA activation, and subsequent vali

66 Rho GTPases (Cdc42 and Rho1-Rho5) and seven Rho GEFs (Scd1, Rgf1-Rgf3, and Gef1-Gef3).

67 In particular, Gef3, the smallest Rho GEF, was barely studied.

68 ise inner nuclear placement relies on SPIKE1 Rho-GEF, SUPERCENTIPEDE1 Rho-GDI, and ACTIN7 (ACT7) func

69 Thus, our studies show that Rho GEFs can serve as selective targets of small chemica

70 Structural requirements for function of the Rho GEF (guanine nucleotide exchange factor) regulator o

71 re essential for cell transformation via the Rho GEF activity or cytoskeletal targeting function.

72 ion of RhoA activity involves ICAM-1 and the Rho GEFs Ect2 and LARG.

73 Both the Rho-GEF domain and its binding region on RanBP9 bind dir

74 However, the requirements for the Rho-GEF and PKD-binding domains during development and c

75 y various modulatory proteins, including the Rho-GEF Lfc.

76 events to create mutant mice that lacked the Rho-GEF and/or the protein kinase D-binding domains.

77 was increased similarly in mice lacking the Rho-GEF and PKD-binding domains and wild-type controls.

78 f DHR-1 in dynamic membrane targeting of the Rho-GEF activity of Dock180.

79 protein 9 (RanBP9) as a novel ligand of the Rho-GEF domain and showed that binding is specific, with

80 We report that overexpression of the Rho-GEF domain specifically inhibits the incorporation o

81 e EphB receptor induces translocation of the Rho-GEF kalirin to synapses and activation of Rac1 and i

82 ssembly and the nuclear sequestration of the Rho-GEF Pebble.

83 s the protein levels and localization of the Rho-GEF Rgf3, which in turn modulates active Rho1 levels

84 be required for appropriate targeting of the Rho-GEF.

85 We have studied the Rho-GEF domain of obscurin to understand its roles in mo

86 These results indicate that the Rho-GEF and PKD-binding domains of AKAP13 are not requir

87 during myofibrillogenesis indicated that the Rho-GEF domain assembles into sarcomeres before RanBP9,

88 Our results suggest that the Rho-GEF domain interacts with RanBP9 and that both can i

89 One protein responsive to UBE3A was the Rho-GEF pebble (pbl).

90 ry domain that mediates interaction with the Rho-GEF obscurin.

91 the Rho family of guanosine triphosphatases (Rho GEF GTPases), as a protein interacting with harmonin

92 e domain, several spectrin-like domains, two Rho GEF domains each containing a Dbl-homology (DH) and

93 nteracts physically and/or functionally with Rho GEFs; however this does not appear to lead to or res