ライフサイエンスコーパス: S. epidermidis

1 S. epidermidis agr reporter strains were developed for e

2 S. epidermidis biofilms preferentially form on abiotic s

3 S. epidermidis contains the cap operon, encoding the pol

4 S. epidermidis has the ability to attach to indwelling m

5 S. epidermidis isolates were collected from 104 patients

6 s of the infecting organisms, we examined 31 S. epidermidis NVE and 65 PVE isolates, as well as 21 is

7 phic demonstrated only two alleles in the 33 S. epidermidis isolates analyzed, corresponding to the p

8 A total of 1559 cultures yielded 565 S. epidermidis isolates; 254 of 548 typed isolates (46%)

9 att site of both an isogenic S. aureus and a S. epidermidis recipient.

10 Limited information is available about S. epidermidis proteins that are expressed upon transiti

11 Additionally, S. epidermidis sar fragments could restore hemolysin pro

12 am-positive pathogens, with activity against S. epidermidis that equals that of the currently prescri

13 nt antimicrobial inhibitory activity against S. epidermidis was maintained for 16 days.

14 tential targets for drug development against S. epidermidis infections.

15 n of adaptive immunity to protection against S. epidermidis challenge was complicated by a highly eff

16 idis accounted for only 1 death (0.5% of all S. epidermidis episodes) (P < 0.001).

17 d PMN migration into fibrin gels and allowed S. epidermidis to increase by approximately 300% in 4 h,

18 me, and performed expression profiling of an S. epidermidis biofilm.

19 more effective at killing P. aeruginosa and S. epidermidis at basic pH values (pH = 9) compared to a

20 ogenesis of biofilm-associated S. aureus and S. epidermidis and may contribute to the chronic nature

21 Both Staphylococcus aureus and S. epidermidis are capable of forming biofilm on biomate

22 Staphylococcus aureus and S. epidermidis are major causes of infection related to

23 pletely inhibit drug-resistant S. aureus and S. epidermidis biofilms.

24 been identified that can bind S. aureus and S. epidermidis cells and are protective in an infant rat

25 ing epitopes on the surface of S. aureus and S. epidermidis cells.

26 latory differences between the S. aureus and S. epidermidis ferritins, as sefA expression in contrast

27 The S. aureus and S. epidermidis genomes are syntenic throughout their len

28 bacterial load associated with S. aureus and S. epidermidis infection in an acute murine bacteremia m

29 tablished in vitro biofilms of S. aureus and S. epidermidis significantly more so than traditional an

30 lococci, above all Staphylococcus aureus and S. epidermidis, are the most frequent causes of biofilm-

31 cludes adhesins from Staphyloccus aureus and S. epidermidis.

32 ) of lauric acid on P. acnes, S. aureus, and S. epidermidis growth indicate that P. acnes is the most

33 e clearance of bacteremia by E. faecalis and S. epidermidis in mice.

34 promising biologic effects, in both HGFs and S. epidermidis.

35 nting a range of clonal complexes as well as S. epidermidis.

36 is study revealed that healthcare-associated S. epidermidis infection is remarkably clonal.

37 homologs found in other sequenced S. aureus, S. epidermidis and S. carnosus genomes.

38 biofilm formation by Staphylococcus aureus, S. epidermidis and Aggregatibacter actinomycetemcomitans

39 gene was observed for Staphylococcus aureus, S. epidermidis, and S. haemolyticus as well as among mec

40 potential use for normal commensal bacterium S. epidermidis to activate TLR2 signaling and induce ant

41 Both S. epidermidis and S. aureus Tpn catalyzed the conversio

42 We also showed that both S. epidermidis bacterial particles and Embp can directly

43 aneuvers aimed at treating disease caused by S. epidermidis and related staphylococci.

44 the agr system enhances skin colonization by S. epidermidis using a porcine model.

45 n specifically prevents biofilm formation by S. epidermidis and methicillin-resistant S. aureus (MRSA

46 modulins (PSMs) gamma and delta produced by S. epidermidis have an alpha-helical character and a str

47 By showing that PNAG production by S. epidermidis biofilm cells exacerbates host inflammato

48 t the production of PSMgamma and PSMdelta by S. epidermidis can benefit cutaneous immune defense by s

49 ination of quorum-sensing regulation used by S. epidermidis represents a surprising and unusual means

50 aphylococcal strains (S. aureus, S. capitis, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunen

51 hole-cell lysates of S. aureus, S. carnosus, S. epidermidis, S. hominis, S. cohnii, S. lugdunensis, a

52 osthetic valves and intravascular catheters, S. epidermidis NVE is a virulent infection associated wi

53 riminate among previously well-characterized S. epidermidis clinical isolates.

54 Naive mice rapidly cleared S. epidermidis infections from blood and solid organs, e

55 me-wide comparison of clinical and commensal S. epidermidis strains to identify putative virulence de

56 genic form of the ubiquitous human commensal S. epidermidis.

57 Representation of the skin commensal S. epidermidis also significantly increased during flare

58 show here the first evidence of a composite S. epidermidis pathogenicity island (SePI), the product

59 oprotease SepA is required for Aap-dependent S. epidermidis biofilm formation in static and dynamic b

60 We describe S. epidermidis clones that are highly resistant to antib

61 ared with 25% of rats challenged with either S. epidermidis O-47mut1 or O-47mut2 (P=.007).

62 (S. aureus), and Staphylococcus epidermidis (S. epidermidis) with lauric acid yielded minimal inhibit

63 robes, including Staphylococcus epidermidis (S. epidermidis), a Gram-positive bacterium, live inside

64 (S. aureus) and Staphylococcus epidermidis (S. epidermidis).

65 keratin and suggest that SdrF may facilitate S. epidermidis colonization of the skin.

66 uture) driveline exit site were cultured for S. epidermidis before VAD insertion and at 7 times after

67 tated, confirming that Embp is essential for S. epidermidis activity against viral infection.

68 The most significant virulence factor for S. epidermidis is its ability to form a biofilm, which r

69 To identify the proteins necessary for S. epidermidis attachment to collagen, we screened an ex

70 s, we analyzed the genome of biofilm-forming S. epidermidis, constructed a microarray representing it

71 c followed by 9,960 CFUs and 9,900 CFUs from S. epidermidis wild type in BALB/c and CD-1, respectivel

72 A cell surface fraction from S. epidermidis 0-47 grown in rabbit serum to mimic envir

73 rile nontoxic small molecule of <10 kDa from S. epidermidis conditioned culture medium (SECM), but no

74 t recognizing adhesive matrix molecules from S. epidermidis.

75 ed Overnight Gram-Positive panels identified S. epidermidis strains accurately, but the panels perfor

76 rowth of P. aeruginosa, whereas impressively S. epidermidis did not grow at all when treated with a 5

77 In S. epidermidis, disparate environmental signals can affe

78 In S. epidermidis, polysaccharide intercellular adhesin (PI

79 n, and packaging of a novel bacteriophage in S. epidermidis FRI909, as well as attempts to mobilize t

80 ve and ACME-derived ADIs are compensatory in S. epidermidis.

81 ns Aap and SasG mediate biofilm formation in S. epidermidis and S. aureus, respectively.

82 ed protein Aap promotes biofilm formation in S. epidermidis, independently from the polysaccharide in

83 the extracellular lipase originally found in S. epidermidis 9, as a collagen-binding protein.

84 s, leukocidins, and leukotoxins not found in S. epidermidis.

85 least in part, mediates hemagglutination in S. epidermidis.

86 report here the cloning of a sar homolog in S. epidermidis.

87 rulence determinants have been identified in S. epidermidis, which are typically acquired through hor

88 esistance as associated with poor outcome in S. epidermidis ODRI.

89 Integrated plasmids in S. epidermidis carry genes encoding resistance to cadmiu

90 olled by sar in S. aureus are not present in S. epidermidis, an examination of functional and structu

91 The interpromoter region in S. epidermidis differs from its S. aureus counterpart, p

92 The major open reading frame within sar in S. epidermidis is highly homologous (84%) to the S. aure

93 a candidate target of balancing selection in S. epidermidis.

94 other bacterial pathogens, quorum sensing in S. epidermidis thus has a different role during biofilm

95 There is a single quorum-sensing system in S. epidermidis encoded by the agr operon.

96 These data suggest that in S. epidermidis SaeR functions to regulate the transition

97 ts conjugation and plasmid transformation in S. epidermidis.

98 There are three agr types in S. epidermidis strains, but only one of the autoinducing

99 on susceptibility to experimentally induced S. epidermidis disease.

100 ion and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome for the

101 athology induced by a subsequent intravenous S. epidermidis challenge, compared to priming with M10 c

102 i, we sequenced the DNA upstream of the 3-kb S. epidermidis sitABC operon, which Northern blot analys

103 crom into these gels in 6 h and did not kill S. epidermidis when the gels contained heat-inactivated

104 ated that specific secreted, surfactant-like S. epidermidis peptides--the beta subclass of phenol-sol

105 era generated in rabbits immunized with live S. epidermidis 0-47 or with biotin-labeled serum protein

106 ARROWgard catheter when tested against MRSA, S. epidermidis, and E. faecalis (p < or = .002).

107 ex vivo antimicrobial activity against MRSA, S. epidermidis, and E. faecalis compared with the ARROWg

108 ribe the first report of a catalase-negative S. epidermidis strain.

109 e, evidence is provided that in PIA-negative S. epidermidis 1457Deltaica, the metalloprotease SepA is

110 Until now, no S. epidermidis phage genome sequences have been reported

111 fense capability and that S. aureus, but not S. epidermidis, triggers a PLA(2) response in the rabbit

112 ull thickness of the gels and to kill 80% of S. epidermidis in 4 h.

113 ckness of the gels in 6 h, and killed 90% of S. epidermidis in 6 h.

114 The CPID-2 panels identified 85 to 95% of S. epidermidis strains, 76 to 86% of S. hominis strains,

115 n significantly reduce in vitro adherence of S. epidermidis to immobilized collagen.

116 Anti-SdrF antibodies reduced adherence of S. epidermidis to keratin and keratinocytes.

117 Subset analysis of S. epidermidis isolates 2 years after the study period s

118 s then tested using multiple applications of S. epidermidis supernatant, the repetitive inflammatory

119 ehD antibodies can inhibit the attachment of S. epidermidis to immobilized collagen.

120 Blood and 1 to 1,000 CFU of S. epidermidis per ml in stationary or exponential phase

121 buffered saline, incubated with 10(6) CFU of S. epidermidis per ml, and cultured.

122 was confirmed to accelerate the clearance of S. epidermidis bacteremia, but TLR2(-/-)mice could still

123 PFGE demonstrated a predominant clone of S. epidermidis (major subtype A) which was 35.5 times mo

124 lator agr affects the biofilm development of S. epidermidis in an unexpected fashion and is likely in

125 ed BALB/cAnNCrl (BALB/c) male mice, doses of S. epidermidis O-47 wild type, its hemB mutant with stab

126 ent involved in the anti-influenza effect of S. epidermidis.

127 late, and the approximately 2.6-Mb genome of S. epidermidis RP62a, a methicillin-resistant biofilm is

128 tained high-resolution time-series images of S. epidermidis at 20-min intervals.

129 All received injections of S. epidermidis on POD 10.

130 idis genome, new markers for invasiveness of S. epidermidis, and potential targets for drug developme

131 atly improve epidemiologic investigations of S. epidermidis.

132 This work expands our knowledge of S. epidermidis agr system function and will aid future s

133 as essential for key virulence mechanisms of S. epidermidis, namely biofilm formation, colonization,

134 atty acid in the fermentation metabolites of S. epidermidis.

135 Our goal was to develop a murine model of S. epidermidis infection to identify potential vaccine t

136 ion, mediated by PIA, in the pathogenesis of S. epidermidis experimental CVC infection.

137 , mediated by PIA/HA, in the pathogenesis of S. epidermidis experimental CVC-associated infection.

138 , mediated by PIA/HA, in the pathogenesis of S. epidermidis experimental foreign body infection.

139 g the in vivo details of the pathogenesis of S. epidermidis infection.

140 e methods to investigate the pathogenesis of S. epidermidis infection.

141 s important role in the biofilm phenotype of S. epidermidis 1457, in which the Aap protein is process

142 abscess formation by different phenotypes of S. epidermidis in a foreign body infection model is most

143 The taxonomic placement of S. epidermidis strain FRI909 was confirmed by a number o

144 ential vaccine targets for the prevention of S. epidermidis bacteremia.

145 roduction of PS/A and that the properties of S. epidermidis associated with initial bacterial adheren

146 pears to be perturbed by the Esp protease of S. epidermidis.

147 immunoreactive or serum binding proteins of S. epidermidis were identified by mass spectrometry.

148 eages and potential donors and recipients of S. epidermidis were identified in each case.

149 phenotype, the agr quorum-sensing regulon of S. epidermidis was characterized by a genomewide analysi

150 also found that a single B domain repeat of S. epidermidis 9491 retains the capacity to bind to type

151 inactivation altered the metabolic status of S. epidermidis, resulting in a massive derepression of P

152 We created a bioluminescent strain of S. epidermidis and developed a subcutaneous catheter-rel

153 PGA was synthesized by all tested strains of S. epidermidis and a series of closely related coagulase

154 ily adsorbed out by PS/A-positive strains of S. epidermidis and recombinant strains of staphylococci

155 The results indicate that strains of S. epidermidis colonising the gut can cause serious path

156 y therefore, the pathogenicity of strains of S. epidermidis which were isolated from the stool sample

157 to which the population genetic structure of S. epidermidis distinguishes commensal from pathogenic i

158 host tissues, contributing to the success of S. epidermidis as a pathogen.

159 disabling agr likely enhances the success of S. epidermidis during infection of indwelling medical de

160 ed significant alterations to the surface of S. epidermidis, and electron microscopy showed cellular

161 Streptococcus (GAS) but not the survival of S. epidermidis on mouse skin.

162 In contrast to the aps system of S. epidermidis, induction of the aps response in S. aure

163 e to exert a complete bactericidal effect on S. epidermidis and S. aureus strains and maintain steril

164 lin exhibited greater bactericidal effect on S. epidermidis than ceftriaxone.

165 e effect of these molecules was evaluated on S. epidermidis growth rate and HGF viability, gene expre

166 ytometry or immunofluorescence microscopy on S. epidermidis 0-47 grown in nutrient broth or in the pr

167 Eyes infected with either S. aureus or S. epidermidis demonstrated a significant increase in MP

168 Rabbits challenged with either S. aureus or S. epidermidis demonstrated a significant reduction in C

169 gene in 599 cultures containing S. aureus or S. epidermidis was 98.6% sensitive and 94.3% specific co

170 for 69 blood cultures with only S. aureus or S. epidermidis was concordant with susceptibility testin

171 The number of viable S. aureus or S. epidermidis was significantly reduced when incubated

172 istance in a species other than S. aureus or S. epidermidis.

173 o sequences adjacent to msrA on the original S. epidermidis plasmid was investigated.

174 capacity to degrade dermcidin, particularly S. epidermidis SepA.

175 e evidence that the murine epidermis permits S. epidermidis, a skin-specific bacterium, to shape the

176 The peak signatures in the polymorphic S. epidermidis locus were traced to an arcD-like gene ad

177 PIA/HA-positive S. epidermidis 1457 was significantly more likely to cau

178 ultiple phenol-soluble modulins, a potential S. epidermidis virulence factor.

179 s of phenol-soluble modulins (PSMs)--promote S. epidermidis biofilm structuring and detachment in vit

180 Furthermore, PGA protected S. epidermidis from high salt concentration, a key featu

181 (SasG) and accumulation-associated protein (S. epidermidis) promote biofilm formation through their

182 19 controls without enoxaparin; all received S. epidermidis injections on POD 10.

183 is work, we show in vitro that a recombinant S. epidermidis Csm1 cleaves single-stranded DNA and RNA

184 le S. epidermidis, and methicillin-resistant S. epidermidis with sensitivities of 95%, 80%, and 96%,

185 sistant S. aureus, and methicillin-resistant S. epidermidis.

186 s corresponding to five previously sequenced S. epidermidis genes were synthesized and then used to a

187 Importantly, PGA efficiently sheltered S. epidermidis from key components of innate host defens

188 s from the closely related commensal species S. epidermidis and S. saprophyticus.

189 In this study, S. epidermidis TCA cycle mutants were constructed, and t

190 ococcus epidermidis, methicillin-susceptible S. epidermidis, and methicillin-resistant S. epidermidis

191 Here we demonstrate that S. epidermidis secretes poly-gamma-DL-glutamic acid (PGA

192 We demonstrate here that S. epidermidis culture supernatants significantly suppre

193 PIA biosynthesis led us to hypothesize that S. epidermidis is "sensing" disparate environmental sign

194 rease in model clot heterogeneity shows that S. epidermidis can rupture a fibrin clot.

195 Our data suggest that S. epidermidis adjusts its lifestyle to varying requirem

196 In conclusion, these data suggest that S. epidermidis bloodstream infection is cleared in a hig

197 These results suggest that S. epidermidis in the nasal cavity may serve as a defenc

198 study is the first analysis suggesting that S. epidermidis isolates from patients with NVE constitut

199 The S. epidermidis fibrinogen (Fg)-binding adhesin SdrG is n

200 The S. epidermidis sodA gene expressed from a plasmid comple

201 SodM and SodA proteins of S. aureus and the S. epidermidis SodA protein exist as dimers.

202 then used to amplify DNA sequences from the S. epidermidis strains by using PCR.

203 hat there are at least five Sir boxes in the S. epidermidis genome and at least three in the genome o

204 ence time and among the most elevated in the S. epidermidis genome.

205 To further understand the outputs of the S. epidermidis agr system, an RNAIII mutant was construc

206 the attachment or accumulation phases of the S. epidermidis biofilm phenotype.

207 udy revealed high genetic variability of the S. epidermidis genome, new markers for invasiveness of S

208 The properties of the S. epidermidis PSMs suggest that they may contribute to

209 The deduced amino acid sequence of the S. epidermidis sodA was 92 and 76% identical to that of

210 no acid and nucleotide repeat regions of the S. epidermidis surface proteins SdrG and Aap show promis

211 Remarkably, the S. epidermidis sar homolog interacts with an agr promote

212 ar response to STF and OspA-L in addition to S. epidermidis (PSM) Ags, and that engagement of TLR2 tr

213 These results demonstrate Aap contributes to S. epidermidis infection, which may in part be due to A

214 em to study the antigen-specific response to S. epidermidis, we demonstrated that skin colonization d

215 gests that care should be used when treating S. epidermidis infections with cross-inhibiting peptides

216 ence and a molecular characterization of two S. epidermidis phages, phiPH15 (PH15) and phiCNPH82 (CNP

217 ete genomic and molecular description of two S. epidermidis phages.

218 d intravenous catheter with either wild-type S. epidermidis 1457 or its isogenic PIA/HA-negative muta

219 Various S. epidermidis and Staphylococcus aureus strains were ex

220 equire an initial colonization step in which S. epidermidis adheres to the implanted material.

221 While S. epidermidis typically causes indolent infections of p

222 as documented in 75% of rats challenged with S. epidermidis O-47, compared with 12.5% and 25% challen

223 Catheter infection with S. epidermidis occurred in 32% of roll plates and 80% of

224 d catheters of 87.5% of rats inoculated with S. epidermidis O-47, compared with 25% of rats challenge

225 ed in mice challenged intraperitoneally with S. epidermidis biofilm cells of the PNAG-producing 9142

226 o 10(7) per ml were mixed in suspension with S. epidermidis at concentrations varying from 10(3) to 1

227 e was more common in rats inoculated with wt S. epidermidis, compared with AtlE- or PIA-deficient mut

228 Wild-type (wt) S. epidermidis O-47 was significantly more likely to cau