ライフサイエンスコーパス: S. gordonii

1 f P. gingivalis in heterotypic biofilms with S. gordonii.

2 optimal coadhesion between P. gingivalis and S. gordonii.

3 losely related Streptococcal species such as S. gordonii.

4 ve oxidative stress response (osr) operon in S. gordonii.

5 fructose phosphotransferase system (PTS) in S. gordonii.

6 tion of GAPDH may be a similar adaptation by S. gordonii.

7 d by over 60% in an SspA-deficient mutant of S. gordonii.

8 activity of another species, H2O2-producing S. gordonii.

9 slundii grew when coaggregated pairwise with S. gordonii.

10 human saliva-supported biofilm formation by S. gordonii.

11 E. faecalis and L. lactis but not on that of S. gordonii.

12 dependently isolated strains, DL1 and M5, of S. gordonii.

13 t pathway for competence induction exists in S. gordonii.

14 reased in the cdhR mutant after contact with S. gordonii.

15 yed elevated accumulation on a substratum of S. gordonii.

16 ransfer of plasmid DNA from E. faecalis into S. gordonii.

17 low-pH-dependent expression of ADS genes in S. gordonii.

18 DNA from mixed cultures of S. sanguinis and S. gordonii.

19 pathogenic potential of F. nucleatum than of S. gordonii.

20 hotransferases encoded by downstream wefC in S. gordonii 38 and wefH in S. oralis 34.

21 UDP-Gal and UDP-GalNAc for RPS production by S. gordonii 38 depends on the dual specificity of the ep

22 d to identify and partially characterize the S. gordonii 38 RPS gene cluster.

23 efC and wefD in the type 2Gn gene cluster of S. gordonii 38 with wefF and wefG from the type 2G clust

24 hat produced by the wefB-deficient mutant of S. gordonii 38.

25 potently inhibits P. gingivalis adherence to S. gordonii (50% inhibitory concentration = 1.3 microM)

26 ted region, when expressed on the surface of S. gordonii, a commensal organism, binds to soluble and

27 may play an important role in development of S. gordonii-A. naeslundii communities in early dental pl

28 tispecies plaque interactions, the effect of S. gordonii AbpB on S. mutans Gtf-B activity was also te

29 The S. gordonii adc operon, consisting of the four ORFs adcR

30 ordonii and that the transposon insertion in S. gordonii adcR::Tn917-lac had resulted in a polar muta

31 Next, putative S. gordonii adhesins were analyzed for contributions to

32 s the structural and functional component of S. gordonii adhesive fibrils, and they provide a molecul

33 ependent manner by P. gingivalis, but not by S. gordonii, after a 15-min exposure.

34 that results in increased expression of the S. gordonii alpha-amylase-encoding gene amyB.

35 we performed comparative analyses on 14 new S. gordonii and 5 S. sanguinis strains using various bio

36 Contact between S. gordonii and a CdhR mutant resulted in increased tran

37 Importantly, the S. gordonii and B. subtilis enzymes have crystallised in

38 that the abpA gene appears to be specific to S. gordonii and differs from genes encoding amylase-bind

39 analyses described here showed that in both S. gordonii and Escherichia coli Rgg is a positive trans

40 ptional changes that accompany competence in S. gordonii and form a basis for future intra- and inter

41 e examined their subcellular localization in S. gordonii and in Escherichia coli expressing the strep

42 is required for disulfide bond formation in S. gordonii and indicate that this enzyme may represent

43 GspB is glycosylated in the cytoplasm of S. gordonii and is then transported to the cell surface

44 eron is involved in manganese acquisition in S. gordonii and manganese homeostasis and appears to mod

45 nderstanding of the regulation of the ADS in S. gordonii and related organisms is needed to develop w

46 elopment of anti-adhesive agents that target S. gordonii and related streptococci.

47 iximab, protected 45%-88% of animals against S. gordonii and S. aureus IE (P < .05).

48 arides were more common among the strains of S. gordonii and S. mitis examined.

49 hibited the interaction of gp340 with intact S. gordonii and S. mutans cells, respectively.

50 While both S. gordonii and S. mutans were abundant colonizers of ra

51 ever, P. gingivalis grew in combination with S. gordonii and S. oralis, demonstrating its ability to

52 We revealed S. gordonii and S. sanguinis harbor open pan-genomes and

53 sights into the genetic distinctions between S. gordonii and S. sanguinis, which yields understanding

54 cterize the related pheromone determinant in S. gordonii and show that the peptide it encodes, gordon

55 arginine metabolism is tightly regulated in S. gordonii and that arginine is critical for gene regul

56 to mediate the adherence of P. gingivalis to S. gordonii and that the species specificity of adherenc

57 at adcRCBA are cotranscribed as an operon in S. gordonii and that the transposon insertion in S. gord

58 he arginine-responsive regulatory network of S. gordonii and the basis for conditional arginine auxot

59 stress tolerance between the oral commensal S. gordonii and the oral pathogen Streptococcus mutans.

60 In a closed system containing S. gordonii and V. atypica, flow cytometric analysis sho

61 treptococci, GBS), E. faecalis, S. pyogenes, S. gordonii, and E. coli containing pDC123 displayed a b

62 nd to Streptococcus pyogenes, S. agalactiae, S. gordonii, and Escherichia coli.

63 strains within the Streptococcus anginosus, S. gordonii, and S. intermedius species.

64 , and fruI are cotranscribed as an operon in S. gordonii, and the transposon insertion in S. gordonii

65 ycoprotein-binding proteins SspA and SspB of S. gordonii; another had 79% identity with the Lactococc

66 S. gordonii appeared to have an intracellular store of p

67 etion, indicating that arcB levels may limit S. gordonii arginine biosynthesis.

68 trials provide encouragement that the use of S. gordonii as a live mucosal vaccine vector is a feasib

69 The same RPS has now been identified from S. gordonii AT, a partially sequenced strain.

70 mponent system regulated in association with S. gordonii biofilm formation in vitro.

71 response, some of which may be important in S. gordonii biofilm formation.

72 with altered microcolony architecture within S. gordonii biofilms formed in saliva during a time fram

73 causes release of DNA from S. sanguinis and S. gordonii but does not result in obvious lysis of cell

74 demonstrated that cell protein extracts from S. gordonii, but not from A. naeslundii, interfered with

75 S. oralis coaggregated with S. gordonii by an RPS-dependent mechanism, and both stre

76 rst time, direct induction of sspA and -B in S. gordonii by human saliva.

77 Coadhesion between P. gingivalis and S. gordonii can be mediated by the SspB protein of S. go

78 livarius and those of the urease produced by S. gordonii carrying the plasmid-borne ure genes.

79 S. gordonii carrying the urease genes was then demonstra

80 Surprisingly, S. sanguinis and S. gordonii cell integrity appears unaffected by conditi

81 Conversely, aggregation of S. gordonii cells by fluid-phase gp340 was not affected

82 0-fold decrease in the level of abpA mRNA in S. gordonii cells cultured in BHI was noted after the ad

83 spB polypeptides are involved in adhesion of S. gordonii cells to human and bacterial receptors.

84 he 98-kDa protease occurred during growth of S. gordonii CH1 in CDM containing 0.075% total amino aci

85 Following invasion by S. gordonii CH1, HUVEC monolayers showed 63% cell lysis

86 rity of these cloned products to the abpA of S. gordonii Challis ranged from 91 to 96%.

87 ighly similar to Hsa, a protein expressed by S. gordonii Challis that has been characterized as a sia

88 Although Hsa is required for the binding of S. gordonii Challis to sialic acid, most of the Hsa expr

89 Biofilm formation of S. gordonii Challis was characterized using an in vitro

90 Eighteen biofilm-defective mutants of S. gordonii Challis were identified based on Southern hy

91 containing V. atypica expressed GFP; nearby S. gordonii colonies that lacked V. atypica did not expr

92 Caries induction reflected S. mutans or S. gordonii colonization abundance: the former highly ca

93 ein interaction has been proposed to promote S. gordonii colonization at multiple sites within the ho

94 S. gordonii competed with S. sanguinis more effectively

95 Results supported published findings on S. gordonii competence, showing up-regulation of 12 of 1

96 typica, flow cytometric analysis showed that S. gordonii containing the PamyB-'gfp reporter plasmid e

97 plain this observation, we hypothesized that S. gordonii could compete with S. sanguinis to adhere to

98 In both subunits of the S. gordonii crystal structure (1.5 A resolution) the C-t

99 e control protein A gene (ccpA) homolog from S. gordonii, designated regG, was cloned.

100 that were infected with either 10(9) CFU of S. gordonii DL-1 or 10(7) CFU of P. gingivalis 33277 did

101 ) was used together with an antibody against S. gordonii DL1 (anti-DL1).

102 14.5 nm long, which are present on wild-type S. gordonii DL1 (Challis) cells, bind CshA-specific anti

103 Inactivation of scaR in S. gordonii DL1 (Challis) resulted in constitutive derep

104 I/II polypeptides and Hsa in interactions of S. gordonii DL1 (Challis) with host receptors.

105 that Hsa directs primary adhesion events for S. gordonii DL1 (Challis) with immobilized gp340, epithe

106 d on the nature of their coaggregations with S. gordonii DL1 and other oral streptococci.

107 Adhesion of S. gordonii DL1 cells to gp340 was sialidase sensitive,

108 then it was linearized and transformed into S. gordonii DL1 for allelic replacement.

109 similarly to sHA, yet 10- to 50-fold excess S. gordonii DL1 reduced binding of S. sanguinis SK36 by

110 Insertional inactivation of the sspA gene in S. gordonii DL1 resulted in reduced binding of cells to

111 Hsa and AgI/II proteins mediated adhesion of S. gordonii DL1 to human HEp-2 epithelial cells.

112 sion and initial biofilm formation on teeth, S. gordonii DL1 was incubated with saliva-coated hydroxy

113 e the sialic acid-binding lectin activity of S. gordonii DL1 with a specific fibrillar antigen, which

114 ion properties of an isogenic sspB mutant of S. gordonii DL1, an sspAB double mutant, and a previousl

115 Five strains, including S. gordonii DL1, caused severe disease, while the other

116 ater biofilm formation on sHA than wild-type S. gordonii DL1.

117 fy the homologous DNA in a fosmid library of S. gordonii DL1.

118 much greater recovery of rifampin-resistant S. gordonii DLl than of streptomycin-resistant S. gordon

119 S. gordonii does not appear to be a good candidate for r

120 In contrast, S. gordonii early CSP-responsive genes were not preceded

121 The arginine deiminase system (ADS) of S. gordonii enables cells to produce, ornithine, ammonia

122 We also identified determinants in S. gordonii encoding a signal peptidase and an Eep-like

123 preparation for clinical trials to evaluate S. gordonii engineered to express group A streptococcal

124 ssory Sec system in E. coli matched those in S. gordonii, establishing the validity of this approach.

125 The srtA mutant S. gordonii exhibited a marked reduction in quantity of

126 Thus, A. naeslundii stabilizes S. gordonii expression of arginine biosynthesis genes in

127 isolated from the same intraoral sites, yet S. gordonii fails to be excluded and survives as a speci

128 d to participate in metabolic communication; S. gordonii ferments carbohydrates to form lactic acid,

129 However, infection with 10(9) CFU of S. gordonii followed by 10(7) CFU of P. gingivalis induc

130 ntration of approximately 12 +/- 5 muM above S. gordonii, followed by a gradual decrease in H2O2 conc

131 S. gordonii, and the transposon insertion in S. gordonii fruK::Tn917-lac resulted in a nonpolar mutat

132 ar GAPDH was the major secreted protein from S. gordonii FSS2, an endocarditis strain.

133 together, these results suggest that AbpA of S. gordonii functions as an adhesin to amylase-coated hy

134 Cell fractionation studies with S. gordonii further corroborated these microscopy result

135 biochemical properties and gene sequence of S. gordonii GAPDH are almost identical to those of other

136 at results in increased transcription of the S. gordonii gene amyB, encoding an alpha-amylase.

137 The majority of S. gordonii genes examined were observed to be downregul

138 as used to quantify changes in expression of S. gordonii genes known or thought to be involved in bio

139 y screening a plasmid integration library of S. gordonii, genes were identified that are crucial for

140 S. gordonii glucosyltransferase (GtfG) and amylase-bindi

141 The results suggest that S. gordonii governs the development of heterotypic oral

142 s cultures containing coaggregates, however, S. gordonii grew to high cell density at low arginine co

143 planktonic culture or as a biofilm, whereas S. gordonii grew under both conditions.

144 del, low concentrations of arginine promoted S. gordonii growth, whereas high concentrations (> 5 mM

145 ing the predicted catalytic triad of Asp2 of S. gordonii had no effect upon GspB transport but did re

146 In mixed cultures of S. mutans and S. gordonii harbouring a shuttle plasmid, plasmid DNA tr

147 However, strain FSS2 of S. gordonii has been found to produce several extracellu

148 gulatory network that controls P. gingivalis-S. gordonii heterotypic communities.

149 ssary for platelet aggregation, and modulate S. gordonii-host engagements into biologically productiv

150 n of cdhR is elevated following contact with S. gordonii; however, regulation of cdhR did not occur i

151 donii can be mediated by the SspB protein of S. gordonii; however, the P. gingivalis cognate receptor

152 ibited the binding between P. gingivalis and S. gordonii in a dose-dependent manner up to 86%.

153 S. mutans out-competed S. gordonii in in vivo plaque biofilm.

154 tain normal adhesion and biofilm function of S. gordonii in response to exogenous oxidants therefore

155 with beta-glucoside metabolism may regulate S. gordonii in vitro adhesion, biofilm formation, growth

156 S. gordonii, in contrast, neither was hindered by nor be

157 s significantly impacted by F. nucleatum and S. gordonii included the mitogen-activated protein kinas

158 S. gordonii-infected mice that were subsequently challen

159 duced production of these cytokines, whereas S. gordonii inhibited secretion from the epithelial cell

160 l a multidimensional aspect to P. gingivalis-S. gordonii interactions and establish pABA as a critica

161 In vitro, S. gordonii is conditionally auxotrophic for arginine in

162 Our findings illustrate that H2O2-producing S. gordonii is dominant while the buffering capacity of

163 tion of fructose transport and metabolism in S. gordonii is intricately tied to carbon catabolite con

164 Interaction of Mfa fimbriae with S. gordonii is necessary to initiate signalling through

165 CylA and CylB system by the alpha-haemolytic S. gordonii is presented.

166 Like wild-type S. gordonii, isogenic mutants with mutations in antigen

167 Mutants of S. gordonii lacking components of the CiaRH, ComDE, or V

168 ompetence sigma factor, were found preceding S. gordonii late responsive genes.

169 Nested PCR cloning from an S. gordonii library enabled the isolation and sequence a

170 eus (P < .005) but failed to protect against S. gordonii (<30% protection).

171 and primer extension analyses revealed that S. gordonii luxS is monocistronic.

172 An S. gordonii luxS mutant that did not produce AI-2 was co

173 s strongly (10- to 100-fold) up-regulated in S. gordonii monocultures after 3 h of growth when exogen

174 ound to be coupled with the induction of the S. gordonii natural competence system.

175 ulated singly, S. mutans always out-competed S. gordonii on the teeth, independent of diet, strain of

176 ain the crucial role AbpB appears to play in S. gordonii oral colonization.

177 Thus, Hsa confers a selective advantage to S. gordonii over S. sanguinis in competitive binding to

178 of the high degree of similarity between the S. gordonii paralogues, analysis of SecA-SecA2 chimeras

179 The S. gordonii PepV gene is homologous to the PepV gene fam

180 Overall, F. nucleatum and S. gordonii perturbed the gingival epithelial cell trans

181 Thus, only S. gordonii possessed all traits advantageous for growth

182 These data indicate that S. gordonii produces an extracellular gelatinase/type IV

183 Others have identified S. gordonii promoters that are up-regulated by a pH shif

184 Contact with S. gordonii propagates a tyrosine phosphorylation-depend

185 ral coimmunization of germfree rats with two S. gordonii recombinants expressing N (residues 55 to 14

186 We have investigated genes of S. gordonii required to support a heterotypic biofilm co

187 Comparison of isogenic pairs of S. gordonii revealed a requirement for several surface p

188 Zymographic analysis of wild-type S. gordonii revealed peptidoglycan hydrolase activities

189 ulation, or presence/absence of mutations of S. gordonii's abpA and gtfG genes known to negatively or

190 Probe SSA-3 hybridized to DNA from S. gordonii, S. mitis, S. oralis, S. parasanguinis, and

191 the hlpA genes were cloned from S. pyogenes, S. gordonii, S. mutans, and S. sobrinus, using PCR ampli

192 e HUVEC was exhibited by selected strains of S. gordonii, S. sanguis, S. mutans, S. mitis, and S. ora

193 varius (two strains); and one strain each of S. gordonii, S. sanguis, S. sobrinus, and S. vestibulari

194 o a much lower maximum cell density than did S. gordonii; S. oralis did not grow reproducibly as a bi

195 Comparison of the S. gordonii SecA and SecA2 proteins in vitro revealed th

196 nd biochemical methods to assess whether the S. gordonii SecA2 functions similarly to SecA.

197 To test this theory, S. gordonii secY2, asp4, and asp5 were co-expressed in E

198 In biofilms, S. gordonii selectively expresses the msrA gene.

199 By contrast, an hsa-deficient mutant of S. gordonii showed significantly reduced binding and com

200 gordonii DLl than of streptomycin-resistant S. gordonii SK12 from the hearts of animals coinfected w

201 ease, while the other two strains, including S. gordonii SK12, caused minimal or no disease.

202 Both of the S. gordonii Ssp proteins bound labeled target cells, whe

203 The S. gordonii SspA and SspB polypeptides mediated higher b

204 Thus, the P. gingivalis receptor for S. gordonii SspB is a 100-kDa protein that structurally

205 These results indicate that expression of S. gordonii sspB is positively regulated by the sspA gen

206 Each S. gordonii strain aggregated with human platelets and b

207 roximately 1.5 x 10(9) CFU of SP204(1-1), an S. gordonii strain not bearing vaccine antigens.

208 Adhesin-mediated binding of each S. gordonii strain to PMNs also triggered phagocytosis.

209 rm(AM)] was inserted within the abpA gene of S. gordonii strains Challis and FAS4 by allelic exchange

210 On the contrary, genomic islands of S. gordonii strains contain additional copies of comCDE

211 In contrast, two S. gordonii strains that lacked hemagglutinating activit

212 S. gordonii strains with specific mutations in the regio

213 ied, one of which was exclusively present in S. gordonii strains.

214 utinating activities of several heterologous S. gordonii strains; however, these bacteria were agglut

215 but also glucosyltransferase G (Gtf-G) from S. gordonii supernatants.

216 The S. gordonii surface proteins SspA and SspB are known to

217 restriction fragment from all 13 strains of S. gordonii tested.

218 ently on the sgc protease knockout mutant of S. gordonii than on the parent biofilms.

219 genes developed more abundant biofilms with S. gordonii than the parental strain developed.

220 ory cascade dominantly control phenotypes of S. gordonii that are critical to colonization, persisten

221 fluorescence levels 20-fold higher than did S. gordonii that had not been incubated with V. atypica.

222 An isolated S. gordonii::Tn917-lac biofilm-defective mutant containe

223 Investigation of an S. gordonii::Tn917-lac biofilm-defective mutant isolated

224 An S. gordonii::Tn917-lac biofilm-defective mutant was isol

225 contrast, did not significantly compete with S. gordonii to adhere.

226 owed that attachment of A. naeslundii and of S. gordonii to glass flowcells was enhanced by a salivar

227 d Asp5 are necessary for optimal adhesion of S. gordonii to glycoproteins gp340 and fibronectin, know

228 sole nutrient showed that V. atypica caused S. gordonii to increase expression of a PamyB-'gfp trans

229 al constraint against S. sanguinis, enabling S. gordonii to persist within the oral cavity, despite t

230 a shuttle plasmid, plasmid DNA transfer from S. gordonii to S. mutans was observed in a CSP and mutac

231 Asp3 also resulted in an impaired ability of S. gordonii to secrete GspB.

232 ribution of GspB and Hsa to the adherence of S. gordonii to selected glycoproteins.

233 Thus, the ability of S. gordonii to survive in PMNs following adhesin-mediate

234 The capacity of S. gordonii to synthesize arginine was assessed using a

235 indicated by transformation frequencies, the S. gordonii transcriptome was analyzed at various time p

236 Comparison of CSP-induced S. gordonii transcriptomes to results published for Stre

237 s demonstrated an increased DNA release from S. gordonii upon addition of the partially purified muta

238 S. sanguinis and S. gordonii used oxygen availability and the differentia

239 To simulate pioneer colonization of teeth, S. gordonii V288 was incubated with sHA for 4 h in THB w

240 formation, a plasmid integration library of S. gordonii V288 was used.

241 ults support the potential usefulness of the S. gordonii vectors expressing P. gingivalis fimbrillin

242 delivery of P. gingivalis FimA epitopes via S. gordonii vectors resulted in the induction of FimA-sp

243 analysis, the entire accessory Sec system of S. gordonii was expressed in Escherichia coli.

244 and maximum AI-2 induction was detected when S. gordonii was grown in the presence of serum and carbo

245 Biofilm formation by S. gordonii was observed to be influenced by the presenc

246 the expression of argC, argG, and pyrA(b) in S. gordonii was partially up-regulated although arginine

247 S. gordonii was recently shown to express a second Ssp p

248 ted in a dose-dependent manner while that of S. gordonii was unaffected.

249 In coaggregation assays, SspB from S. gordonii was unique in mediating coaggregation with o

250 To study saliva-regulated gene expression in S. gordonii, we used random arbitrarily primed PCR (RAP-

251 alysis, the 20-kDa AbpA protein is unique to S. gordonii, whereas the 82-kDa AbpB protein appears to

252 ontrast, type 1 fimbriated A. naeslundii and S. gordonii, which bound purified proline-rich proteins

253 of both species and well-defined mutants of S. gordonii with interrupted abpA and gtfG genes were st

254 Furthermore, communities of S. gordonii with P. gingivalis or with A. actinomycetemc

255 x-prolyl dipeptidyl-peptidase (Sg-xPDPP, for S. gordonii x-prolyl dipeptidyl-peptidase), produced in