ライフサイエンスコーパス: S. lividans

1 S. lividans 66 TK24 expressing nec1 does not produce tha

2 has been constructed and overexpressed in a S. lividans expression system.

3 transition to a circular form, we isolated a S. lividans chromosomal gene (tpgL) that we found specif

4 and heterologous hosts, such as S. albus and S. lividans.

5 llele in the chromosome of S. coelicolor and S. lividans.

6 used severe growth inhibition of E. coli and S. lividans, but RelE1sca had no toxic effect.

7 n of neomycin resistance in both E. coli and S. lividans.

8 ction in the absence of glucose increased as S. lividans cells entered stationary phase, but unlike A

9 terating this phenotypic distinction between S. lividans and S. coelicolor.

10 ified DNA sequence (ADS5.7) found in certain S. lividans mutants.

11 ymes by either wild-type E2 or an endogenous S. lividans CMCase.

12 ene knockout were employed in the engineered S. lividans strain to identify the P450 monooxygenase Ge

13 vectors in which doxA was poorly expressed, S. lividans catalyzed the reduction of daunomycin and ot

14 initiation factors (IF1, IF2, and IF3) from S. lividans were isolated and included in toeprint and f

15 one were each expressed in and purified from S. lividans and had very low catalytic activity on swoll

16 Anaerobically purified WhiB7 from S. lividans was dimeric and contained 2.1 +/- 0.3 and 2.

17 in membrane fractions of both surface-grown S. lividans, which mate readily, and of cells grown in l

18 1, is sufficient for necrotizing activity in S. lividans 66 TK24.

19 Also in S. lividans, TylP negatively controlled the tylQ promote

20 te that the production of this antibiotic in S. lividans grown on agar can be modulated by carbon sou

21 to genes merR, merT, merP, and orfIV, as in S. lividans 1326.

22 t pathways that regulate ACT biosynthesis in S. lividans and further demonstrate that the production

23 t that the occurrence of ACT biosynthesis in S. lividans is determined conditionally by the carbon so

24 the regulation of antibiotic biosynthesis in S. lividans TK24 is proposed.

25 rms ranging in size from 10 to 6 kDa both in S. lividans and S. cyanogenus.

26 o background of AMO activity was detected in S. lividans cells without amoABCD and expression of AMO

27 Expression of the pel gene in S. lividans TK24 resulted in high pectate lyase activity

28 RNA I and 9S ribosomal RNA was identified in S. lividans cell extracts.

29 with, TpgR proteins for pSLA2 maintenance in S. lividans.

30 Posttranslational maturation in S. lividans of both the wild-type berninamycin prepeptid

31 that the intermycelial transfer of pIJ101 in S. lividans is complete by the onset of cellular differe

32 utive expression of fdmR1; FDM production in S. lividans could be enhanced further by overexpressing

33 ufficient to allow valanimycin production in S. lividans TK24.

34 , afsR and afsR2, activate ACT production in S. lividans, indicating that this streptomycete encodes

35 reduced expression from the tylS promoter in S. lividans.

36 (pIJ101) is expressed as a 10-kDa protein in S. lividans that is immediately processed to a mature 6-

37 ble stabilisation of a mobile region seen in S. lividans CelB2.

38 gether with the time of appearance of Tra in S. lividans membranes, indicate that the intermycelial t

39 s plasmid pSB24.1 is deleted upon entry into S. lividans to form pSB24.2, a nonconjugative derivative

40 ns of these vector sets were introduced into S. lividans, resulting in strains producing a wide range

41 of pglYZ and that introduction of pglYZ into S. lividans is not sufficient to confer a Pgl+ phenotype

42 established and the polyphosphate content of S. lividans TK24 and the ppk mutant was determined.

43 otein throughout cellular differentiation of S. lividans, which leads to maximum KilB concentrations

44 binant AMO activity in cell-free extracts of S. lividans was stimulated by the addition of NADH and p

45 We found that the growth of S. lividans on solid media containing glucose prevents A

46 inear form and also prevented propagation of S. lividans cells that contain linear, but not circular,

47 h deletion of csoR has only minor effects on S. lividans development when grown under high copper con

48 We report that the Streptomyces species S. lividans and S. coelicolor, morphologically complex g

49 Southern blots indicated that S. lividans does not contain homologues of pglW or pglX.

50 otein with tra mRNA concentration during the S. lividans life cycle indicated that the disappearance

51 Expression of Tra during the S. lividans life cycle was temporally regulated and was

52 lar plasmid inheritance by spores during the S. lividans life cycle.

53 to initiate transcription correctly from the S. lividans galP1 and galP2 promoters, and the Bacillus

54 However, the S. lividans host could be engineered to produce FDMs via

55 nsertion of the kilB gene of pIJ101 into the S. lividans chromosome in cells lacking the pIJ101 KorB

56 XYZ was able to confer the Pgl+ phenotype to S. lividans implying that these four genes constitute th

57 During mating with S. lividans, SLP1(int) can excise, delete part of imp, a