ライフサイエンスコーパス: SAM domain

1 e UBC-9 through its evolutionarily conserved SAM domain.

2 egion, a PEST domain, and a carboxy-terminal SAM domain.

3 lays a substantial drop after removal of the SAM domain.

4 cient PRE recruitment requires an intact Scm SAM domain.

5 6 N-terminal tyrosines (3Y) dependent on the SAM domain.

6 h a reaction that requires an intact radical SAM domain.

7 clusters was impaired by the deletion of the SAM domain.

8 eract with both Yan and Pnt-P2 through their SAM domains.

9 zosaccharomyces pombe and interact via their SAM domains.

10 Yan and Mae interact through their SAM domains.

11 egion of interaction to their respective SPM/SAM domains.

12 dules when searching for binding partners of SAM domains.

13 ng a new mode of oligomeric organization for SAM domains.

14 o study the behavior of phosphorylated EphA2 SAM domains.

15 coiled-coil domains followed by three tandem SAM domains.

16 iated by the N-terminal sterile alpha motif (SAM) domain.

17 elta and eta, contain a sterile alpha motif (SAM) domain.

18 ity of its intraluminal sterile alpha-motif (SAM) domain.

19 tumor domain (MBT) and sterile alpha motif (SAM) domains.

20 racted from the substrate by the two radical SAM domains, (2) the second tyrosine-derived product, (3

21 It contains a sterile-alpha motif (SAM) domain, 3 phosphotyrosine motifs, a proline-rich re

22 ructure reveals an unusual makeup in which a SAM domain, a common protein-protein interaction module,

23 SOP-2 contains a SAM domain, a self-associating protein domain found in o

24 In this context, dNTP triphosphohydrolase SAM domain and HD domain-containing protein 1 (SAMHD1) h

25 SAM domain and HD domain-containing protein 1 (SAMHD1),

26 those required for viral cDNA synthesis by a SAM domain and HD domain-containing protein 1 (SAMHD1)-i

27 ot part of the hydrophobic core of the EphB2-SAM domain and is conserved for functional reasons.

28 ild-type EphA4 and a mutant lacking both the SAM domain and PDZ binding motif were constitutively tyr

29 ntifies the domain as a sterile alpha-motif (SAM) domain and shows a propensity to oligomerize.

30 in part to the nucleotidase activity of the SAM-domain and HD-domain containing protein (SAMHD1), wh

31 of 871 amino acids carrying an SH3 domain, a SAM domain, and a PH domain.

32 id substitutions in the sterile alpha motif (SAM) domain, and are predicted to affect protein--protei

33 The HIV-1 restriction factor SAM domain- and HD domain-containing protein 1 (SAMHD1)

34 SAMHD1 (SAM domain- and HD domain-containing protein 1) is a dGT

35 a four-helix bundle (H2-H5), resembling the SAM domain, appended with two additional helices (H0-H1)

36 SAM domains are known to form homo- and hetero-oligomers

37 ein interaction studies demonstrate that the SAM domains are necessary and sufficient to mediate SARM

38 lex interactions between the coiled-coil and SAM domains are thought to create liprin scaffolds, but

39 Although SAM domains are very commonly found in eukaryotic protei

40 Sterile alpha motif (Sam) domains are protein interaction modules that are im

41 ce, reduces self-association of the isolated SAM domain as well as high molecular mass complex format

42 tingly, deletion of the sterile alpha motif (SAM) domain at the N terminus dramatically reduced the u

43 unique in possessing a sterile alpha motif (SAM domain) at their C-terminal ends.

44 The n-NafY SAM domain binds apo-NifDK.

45 We show that not only the SAM domain but also the N-terminal tail engages in the D

46 he resulting transformants indicate that the SAM domain but not the RA domain is essential for the fu

47 ive-helix-bundle architecture of traditional SAM domains, but has additional short helices at N and C

48 These results indicate that SAM domains can create a variety of oligomeric architect

49 providing the first clear demonstration that SAM domains can polymerize.

50 SAM domains can self-associate to form higher-order stru

51 In this study, we have identified SAM domain-carrying non-receptor tyrosine kinase, activa

52 SAM domain containing proteins have been shown to requir

53 Finally, the human HemW orthologue radical SAM domain-containing 1 (RSAD1) stably bound heme.

54 Byr2 and Ste4 are two SAM domain-containing proteins in the mating pheromone r

55 acterize the Drosophila sterile alpha-motif (SAM) domain-containing protein Caskin, which shares homo

56 Furthermore, the H5 alpha helix within the SAM domain contributed to self-association.

57 ur results also suggest a mechanism by which SAM domains could mediate the spreading of transcription

58 n, we generated SLP-76 knockin mice with the SAM domain deleted.

59 Our results indicate that SAM domain deletion (EphA2DeltaS-GFP) increases oligomer

60 These results suggest that SAM domain deletion induced constitutive activation of E

61 The SAM domain deletion mutant, EphA2DeltaS-GFP, also underw

62 tion and abrogated by the deletion of SLP-76 SAM domain (DeltaSAM) or mutation of Tyr-113, Tyr-128, a

63 tations on Mae that specifically disrupt its SAM domain-dependent interactions with Yan disable the d

64 gated the potential role of zinc in DGKdelta SAM domain (DGKdeltaSAM) assembly.

65 Previous crystal structures of an EphA4-SAM domain dimer and a possible EphB2-SAM oligomer both

66 ins green fluroscent protein (GFP) or EphB2 (SAM domain) displayed markedly increased growth rates wi

67 horylation levels upon deletion of the EphA2 SAM domain (EphA2DeltaS) in DU145 and PC3 prostate cance

68 been taken over by a different branch of the SAM domain family during the evolution of nematodes.

69 n EphB2-SAM domain that has the same overall SAM domain fold yet has no substantial intermolecular co

70 s a three-dimensional docking surface on its SAM domain for the MAP kinase, Rolled.

71 Here we show that the TEL-SAM domain forms a helical, head-to-tail polymeric struc

72 The crystal structure of the SAM domain from an Eph receptor tyrosine kinase, EphB2,

73 nd its possible mode of interaction with the Sam domain from the EphA2 receptor.

74 ubstrate receptor, DCAF1, and N-terminal and SAM domains from mandrill SAMHD1.

75 We determined that the amino-terminal SAM domain functions as an autoinhibitory domain of intr

76 We showed previously that the SAM domain has two binding regions that mediate dimer an

77 fection in macrophages involves the cellular SAM domain HD domain-containing protein 1 (SAMHD1).

78 dissociation process of the EphA2-SHIP2 SAM-SAM domain heterodimer complex using unrestrained all-at

79 at of dNumb developmental regulators and two SAM domains homologous to those in the C-terminal tail o

80 tantly, aberrant C-terminal extension of the SAM domain in bpk mutant Bicc1 phenocopied these defects

81 escence study to investigate the role of the SAM domain in EphA2 function.

82 Aberrant localization of the Scm-SAM domain in long contiguous regions on polytene chromo

83 identified for the first time a role for the SAM domain in mediating SLP-76 self-association for T-ce

84 The presence of a SAM domain in NafY was unexpected and could not be infer

85 structural basis for the versatility of the SAM domain in protein and RNA-recognition.

86 ve been extensively studied, the role of the SAM domain in SLP-76 function is not known.

87 tirely different from that of the equivalent SAM domain in SRF and MCM1, accounting for the absence o

88 ins that become ordered upon binding, the EF-SAM domain in the stromal interaction molecule (STIM) 1

89 binding by an adaptor and suggest a role for SAM domains in clathrin-mediated endocytosis.

90 However, the functions of SAM domains in Eph receptors remain elusive.

91 SAM domains in some systems have been shown to self-asso

92 The presence of PDZ and SAM domains in the KS5 protein suggests that it may act

93 binding, the solution structure of the Vts1 SAM domain, in the presence of a specific target RNA, ha

94 In contrast, a deletion of the SAM domain induces a complex hindlimb defect associated

95 on these results, we conclude that the EphA2 SAM domain inhibits kinase activity by reducing receptor

96 A heterotypic Sam-Sam domain interaction is mediating this process.

97 via a heterotypic sterile alpha motif (SAM)-SAM domain interaction, leading to regulation of EphA2 i

98 Altogether, our data demonstrated that the SAM domain is indispensable for optimal SLP-76 signaling

99 hening of Mtrm::Polo binding mediated by the SAM domain is necessary to prevent meiotic catastrophe a

100 Although Mtrm's C-terminal SAM domain is not required to rescue the chromosome segr

101 Chimeric constructs in which the SOP-2 SAM domain is replaced with that derived from fruit fly

102 G proteins, but not those in which the SOP-2 SAM domain is replaced with the SAM domains of non-PcG p

103 1 are associated with its HD domain, but the SAM domain is required for maximal activity and nucleic

104 The sterile alpha motif (SAM) domain is a protein interaction module that is pres

105 The sterile alpha motif (SAM) domain is a protein module found in many diverse si

106 modulated by their evolutionarily conserved SAM domain, is essential to their physiological repressi

107 ires the Scm C-terminal sterile alpha motif (SAM) domain, is crucial for the efficient sumoylation of

108 in vivo, we show that a sterile alpha-motif (SAM) domain located at the C terminus of Mtrm increases

109 loses Ca(2+) from EF hand, its intraluminal SAM domain may change conformation, and via glycosylatio

110 induce these phenotypes, suggesting that the SAM domain may negatively regulate some aspects of EphA4

111 daptor protein that interacts with Lar via a SAM domain-mediated interaction.

112 SAM domain-mediated polymerization of EN leads to consti

113 Introduction of SAM domain missense mutations that restrict Yan to a mon

114 n of messenger RNA encoding the Elp3 radical SAM domain mutant, but not the HAT domain mutant, into M

115 Here, we have investigated how these SAM domain mutations affect EPHA2 activity.

116 We showed that the SAM domain mutations dramatically destabilized the EPHA2

117 Coexpression of combinations of SAM domain mutations that permit the formation of Yan di

118 Tu et al. showed that the isolated SAM domain of Byr2 binds a fragment of Ste4 that contain

119 unction alleles indicate that the N-terminal SAM domain of Ckn mediates its interaction with Lar.

120 The SAM domain of DGKdelta1 forms helical polymers that are

121 hermal titration calorimetry) studies on the Sam domain of Ship2 revealing its three-dimensional stru

122 that glycosylation sites in the ER-resident SAM domain of STIM1 are essential for initiation of CIF

123 ts fuse a potent oligomerization module, the SAM domain of TEL, to a variety of tyrosine kinases or t

124 Taken together, our studies suggest that the SAM domain of the EPHA2 protein plays critical roles in

125 Ship2 that retains binding affinity for the Sam domain of the EphA2 receptor.

126 earing loss, due to mutations disrupting the SAM domain of the protein kinase ZAK.

127 ydG showed that the highly conserved radical-SAM domains of both HydE and HydG and the GTPase domain

128 ch the SOP-2 SAM domain is replaced with the SAM domains of non-PcG proteins, confer appropriate in v

129 Here, we show that the individual SAM domains of Ste4 and Byr2 are monomeric at low concen

130 The lumenal EF-hand and SAM domains of STIM1 are believed to initiate oligomeriz

131 Here we examine the interaction between the SAM domains of the polycomb group proteins polyhomeotic

132 ions in the cytoplasmic sterile-alpha-motif (SAM) domain of human EPHA2 on chromosome 1p36 have been

133 The sterile alpha-motif (SAM) domain of Mst50 was essential for its interaction w

134 ted by mutations in the sterile alpha-motif (SAM) domain of p63 that are associated with ankyloblepha

135 that the N-terminal sterile alpha motif (or SAM) domain of SMSr drives self-assembly of the protein

136 The sterile alpha motif (SAM) domain of the ephrin receptor tyrosine kinase, EphA

137 the SAMHD1 N-terminal tail and the adjacent SAM domain or the C-terminal tail proceeding the HD doma

138 We find that the EF-SAM domain partially unfolds and dimerizes cooperatively

139 A third system pairs a radical SAM domain peptide maturase with selenocysteine-containi

140 roles in protein-protein interactions, some SAM domains play crucial roles in RNA binding.

141 regulated enhancer controls expression of an SAM domain protein that confers survival in anemia.

142 (Samd14-Enh) encoding a sterile alpha motif (SAM) domain protein.

143 ure, the N-terminal peptide arm of the EphB2-SAM domain protrudes out from the core of the molecule,

144 Moreover, a mutant DGKdelta containing a SAM domain refractory to zinc binding diminishes the for

145 cted structure modeling and then mutated the SAM domain residues which in this model were predicted t

146 nt microscale thermophoresis of the isolated SAM domain (residues 1-78) revealed evidence of dimers a

147 The Vts1 SAM domain retains the "core" five-helix-bundle architec

148 A K78E missense mutation within the SAM domain, revealed a genetic interaction between ubc2

149 merizing TNKS and TNKS2 sterile alpha motif (SAM) domains, revealing versatile head-to-tail interacti

150 8 to Ala-8 mutation and found that the EphB2-SAM domain structure and stability were only slightly al

151 leucine zipper (Ste4-LZ) domain as well as a SAM domain, suggesting that Byr2-SAM and Ste4-SAM may fo

152 the catalytic [Fe4S4] cluster in the radical SAM domain, surprisingly, does not abolish the inhibitor

153 solved a new crystal form of the human EphB2-SAM domain that has the same overall SAM domain fold yet

154 nd genetic analysis that it is primarily the SAM domain that interacts specifically with the appropri

155 a structural model of the Ca(2+)-unbound EF-SAM domain that is consistent with a wide range of evide

156 ne-rich region that binds to cortactin and a SAM domain that mediates multimerization.

157 nd that the interface maps to regions of the SAM domains that are known to be important for the forma

158 motif) domain is a member of a new class of SAM domains that specifically bind RNA.

159 a small protein with a sterile alpha motif (SAM) domain that can physically interact with the scaffo

160 us of Shank3 contains a sterile alpha motif (SAM) domain that is essential for its postsynaptic local

161 ere, we examine the response of the STIM1 EF-SAM domain to changes in Ca(2+) concentration using math

162 that full-length Yan self associates via its SAM domain to form higher-order complexes in living cell

163 polymerizes through its sterile alpha motif (SAM) domain to assemble large protein complexes.

164 Moreover, mutation of a conserved SAM domain tyrosine to phenylalanine (Y928F) enhanced th

165 l activity of viperin depends on its radical SAM domain, which contains conserved motifs to coordinat

166 The BAR protein contains a SAM domain, which is required for its interactions with

167 145, "3Y") as well as a sterile alpha motif (SAM) domain whose function is unclear.

168 93F, Y587E/Y593E), kinase domain (Y734F), or SAM domain (Y929F) inhibited ephrin-A1-induced vascular

169 is trimeric and, when included with the Ste4-SAM domain, yields a 3:1 Ste4-LZ-SAM:Byr2-SAM complex wi