ライフサイエンスコーパス: SHFV

1 SHFV encodes three more ORFs on its genome than the othe

2 SHFV is a member of a new virus family which includes th

3 SHFV is unique among arteriviruses in having three N-ter

4 SHFV PLP1gamma is able to cleave at both downstream and

5 SHFV replicated in >90% of macaque MPhis but in only app

6 acilitated by next-generation sequencing, 96 SHFV body TRSs were identified that were functional in b

7 However, little is known about SHFV's ecology and molecular biology and the mechanism b

8 han macaque cultures both prior to and after SHFV infection.

9 ultures with human IL-10 before and/or after SHFV infection decreased production of IL-6, IL-1beta, a

10 Although SHFV infected approximately 50% of macaque and baboon mD

11 similar positions in the LDV-C 3'(-)NCR and SHFV 3'(-)209 RNAs.

12 g patterns were obtained with uninfected and SHFV-infected extracts, indicating that the four protein

13 only in cis at a single downstream site, but SHFV PLP1gamma can cleave at both the downstream nsp1gam

14 and in Alamogordo in 1989 were caused not by SHFV but by two novel divergent arteriviruses.

15 In baboon but not macaque cell cultures, SHFV infection upregulated IL-10R1, a subunit of the IL-

16 e and respiratory syndrome virus, diminished SHFV replication, identifying CD163 as an important SHFV

17 During SHFV infection, we detected signatures of selection on O

18 y mechanism and expanded coding capacity for SHFV, which may also be characteristic of other nidoviru

19 The results of this study shed light on how SHFV enters its target cells.

20 plication, identifying CD163 as an important SHFV entry component.

21 All known SHFV variants are monophyletic and share three open read

22 tion reactions done with wild-type or mutant SHFV nsp1 constructs.

23 We describe two new SHFV variants subclinically infecting wild African red-t

24 the in vitro reactions, Western blotting of SHFV-infected, MA104 cell lysates with SHFV nsp1 protein

25 Cys63 was identified as the catalytic Cys of SHFV PLP1alpha and is adjacent to an Ala instead of the

26 fferential infection outcome, the targets of SHFV infection, macrophages (MPhis) and myeloid dendriti

27 Phospholipases A2 and D had no effect on SHFV entry.

28 n contrast, we detected selection focused on SHFV ORFs 5a and 5, which encode putative membrane prote

29 ine, and concanamycin A dramatically reduced SHFV entry efficiency, whereas the macropinocytosis inhi

30 , suggesting a role for IL-10 in suppressing SHFV-induced proinflammatory cytokine production in maca

31 of in vitro autoprocessing of an N-terminal SHFV nonstructural 1a polypeptide fragment showed that e

32 rious cellular pathways, we demonstrate that SHFV invades cells by low-pH-dependent, actin-independen

33 chalasin B, and cytochalasin D indicate that SHFV does not hijack the actin polymerization pathway.

34 Our experiments indicate that SHFV enters target cells by low-pH-dependent endocytosis

35 nd electron microscopy results indicate that SHFV entry occurs by a dynamin-dependent clathrin-mediat

36 Comparative sequence analysis suggested that SHFV ORFs 2a, 2b, and 3 are related to ORFs 2 through 4

37 Evidence which suggests that SHFV ORFs 4 through 6 are related to ORFs 2a through 3 a

38 The SHFV genome encodes nine ORFs that are presumed to be ex

39 The SHFV PLP1alpha catalytic Cys63 is unique among arterivir

40 To date, the SHFV life cycle is almost completely uncharacterized on

41 lecule identified as an entry factor for the SHFV-related porcine reproductive and respiratory syndro

42 TRSs were identified for the majority of the SHFV 3' ORFs, and four previously identified TRSs were f

43 nd sequenced 6,314 nt from the 3' end of the SHFV genome.

44 Here, we describe the first steps of the SHFV life cycle.

45 184 (68-nt sequence) from the 3' end of the SHFV negative-strand RNA.

46 n to be proximal by homology modeling of the SHFV nsp1s on porcine respiratory and reproductive syndr

47 The coding capacity for each of the SHFV ORFs as well as the potential mass, pI and number o

48 HFV PLP1s were predicted by alignment of the SHFV PLP1 region sequences with each other as well as wi

49 lternative products generated by each of the SHFV PLP1s cleaving at sites within the N-terminal regio

50 Several unique features of the SHFV PLP1s were discovered.

51 residues and cleavage sites for each of the SHFV PLP1s were predicted by alignment of the SHFV PLP1

52 This is the first functional study of the SHFV PLP1s.

53 rypsin) abrogated entry, indicating that the SHFV cell surface receptor is a protein.

54 e molecular masses as those that bind to the SHFV 3'(-)209 RNA also bind to the LDV-C 3'(-)NCR RNA an

55 elevating virus C (LDV-C), competed with the SHFV 3'(-)209 RNA in competition gel mobility shift assa

56 asmic extracts formed two complexes with the SHFV 3'(-)209 RNA, and results from competition gel mobi

57 ptide fragment showed that each of the three SHFV PLP1s is active, and the predicted catalytic Cys re

58 The data showed that each of the three SHFV PLP1s is an active protease.

59 ly divergent simian arteriviruses related to SHFV, Mikumi yellow baboon virus 1 (MYBV-1) and Southwes

60 protein 1alpha (MIP-1alpha), in response to SHFV infection were observed in macaque but not baboon c

61 Simian hemorrhagic fever virus (SHFV) causes a fatal hemorrhagic fever in macaques but a

62 Simian hemorrhagic fever virus (SHFV) causes a severe and almost uniformly fatal viral h

63 Simian hemorrhagic fever virus (SHFV) causes highly lethal disease in Asian macaques res

64 aques, while simian hemorrhagic fever virus (SHFV) causes lethal viral hemorrhagic fever.

65 arterivirus simian hemorrhagic fever virus (SHFV) is 209 nucleotides (nt) in length.

66 Simian hemorrhagic fever virus (SHFV) is an arterivirus that causes severe disease in ca

67 al region of simian hemorrhagic fever virus (SHFV) nonstructural polyprotein 1a is predicted to encod

68 with either simian hemorrhagic fever virus (SHFV) or Kibale red colobus virus 1 (KRCV-1) and assesse

69 Simian hemorrhagic fever virus (SHFV) was recently reclassified and assigned to the new

70 arterivirus Simian hemorrhagic fever virus (SHFV).

71 same virus, simian hemorrhagic fever virus (SHFV; Arteriviridae).

72 e the 1960s, simian hemorrhagic fever virus (SHFV; Nidovirales, Arteriviridae) has caused highly fata

73 ng of SHFV-infected, MA104 cell lysates with SHFV nsp1 protein-specific antibodies detected only the