ライフサイエンスコーパス: SHG

1 SHG angular intensity pattern (SHG-AIP) of healthy and p

2 SHG endomicroscopic imaging of ex vivo murine and human

3 SHG microscopy is an emerging microscopic technique for

4 SHG results show that interfacial polarity probed by p-n

5 SHG signals are exquisitely sensitive to the polarizatio

6 SHG spectra report the electronic structure of solutes a

7 SHG together with the Poisson-Boltzmann equation yielded

8 SHG transmission images of collagen fibers were spatiall

9 SHG-AIP with two symmetrical spots is found to be a sign

10 SHG-based three-dimensional reconstructions of Chlamydom

11 Thus, the combined method of 3D SHG imaging and modeling forms an essential foundation f

12 llent substrates for DPE2 and propose that a SHG is the in vivo substrate for DPE2 and AtPHS2.

13 h multiphoton bandpass filters to obtain AF, SHG (collagen bandwidth), and eosin-labeled fluorescence

14 r-simultaneous image acquisition pairs of AF-SHG (+/-eosin coincubation), AF-eosin, and SHG-eosin wer

15 l meshwork for image acquisition pairs of AF-SHG (without eosin coincubation) and SHG-eosin.

16 ral SRS and SHG imaging reveals that not all SHG-active structures with solidlike morphologies can be

17 a universal 3-nitropyrolle nucleotide as an SHG-active label, we monitored the hybridization rate an

18 Glassy K2P2Se6 also exhibits an SHG response without the application of electric field p

19 s of AF-SHG (without eosin coincubation) and SHG-eosin.

20 F-SHG (+/-eosin coincubation), AF-eosin, and SHG-eosin were captured.

21 However, combined hyperspectral SRS and SHG imaging reveals that not all SHG-active structures w

22 y exemplifies the merit of combining SRS and SHG microscopy for an enhanced label-free chemical analy

23 rent anti-Stokes Raman scattering, TPEF, and SHG (SFG) microscopy--allows simultaneous visualization

24 find that all of the reported materials are SHG-active at 1064 nm, with responses ranging from 2.8 t

25 As SHG offers a powerful, high-throughput screening approac

26 ssert that complex optical artifacts such as SHG verniers should be taken into account when applying

27 The average SHG intensity oscillated with wavelength in the backscat

28 changes in fibrosis from the backward SHG (B(SHG)) alone, as only backward-propagating SHG is accessi

29 easure changes in fibrosis from the backward SHG (B(SHG)) alone, as only backward-propagating SHG is

30 sue slices, the ratio of forward to backward SHG signal from large bundles was found to be much large

31 SHG vernier" patterns, regions of bifurcated SHG intensity, are illusory when sarcomeres are staggere

32 The high spatial resolution afforded by SHG microscopy allows for the use of a minibeam collimat

33 field imaging, followed by classification by SHG.

34 static electric fields easily detectable by SHG.

35 y important as collagen deposition imaged by SHG, remain poorly exploited to date.

36 at can also be detected at the bond level by SHG.

37 t agreement with the classifications made by SHG, with measurement times of approximately 1 min and s

38 ntrosymmetric crystal forms were observed by SHG microscopy.

39 es outside of the interfacial zone probed by SHG.

40 In both cases, SHG was able to resolve conformational changes in these

41 the mouse eye was also visible, with a clear SHG signal representing collagen fibers.

42 lso provide details for injecting PEG-coated SHG nanoprobes into zygote-stage zebrafish embryos, and

43 sion geometry (forward propagating, coherent SHG component) around 440 nm (lambda(p) = 880 nm).

44 The glass of APSe(6) exhibits comparable SHG intensities to the top infrared NLO material AgGaSe(

45 Other plant species also contain SHG, DPE2, and alpha-glucan phosphorylase, so this pathw

46 sitional analysis reveal that the cumulative SHG intensity within each image volume and the average c

47 direct fitting of the polarization dependent SHG signal.

48 Phasor projection of Polarization-dependent SHG (muMAPPS) that maps the features of the collagen arc

49 Polarization-dependent SHG measurement and synchrotron X-ray microdiffraction a

50 erimental characterization of myosin-derived SHG intensity profiles within intact zebrafish skeletal

51 ers (nonfibrous) did not show any detectable SHG, indicating a lack of noncentrosymmetric crystalline

52 tly spatially ordered to generate detectable SHG without the use of any fluorescent dye.

53 l background fluorescence, but no detectable SHG.

54 he ratio of the forward-to-backward detected SHG provides a measure of the particle size, suggesting

55 sin; allowing SHIM to characterize different SHG-generating components within a complex biological sa

56 ally resolved due to a coherent, directional SHG component.

57 evements in the field of frequency doubling (SHG) and tripling (third-harmonic generation, THG) in th

58 The optical signal and the PD during SHG imaging of stained cultured Aplysia neurons were exa

59 thesized that bulk optical properties (i.e., SHG and TPF) could be used to predict bulk mechanical pr

60 delivery, scanning, focusing, and efficient SHG signal collection.

61 ield-induced second harmonic generation (EFI-SHG) technique that can directly monitor the dynamic per

62 The EFI-SHG studies confirmed the motion of the water can remove

63 After the contact, the EFI-SHG system visualized briefly three relaxations of the t

64 xibility and material selectivity of the EFI-SHG.

65 Endomicroscopic SHG images of murine cervical tissue sections at differe

66 t chi(3) experiments and resonantly enhanced SHG experiments that probe the ligand-to-metal charge tr

67 The as-prepared glass fibers exhibit SHG and difference frequency generation (DFG) responses

68 ing methods (including organic fluorophores, SHG chromophores, genetic indicators, hybrid, nanopartic

69 rp contrast with most materials designed for SHG purposes, which generally require the use of expensi

70 4, and all-trans-retinol, were evaluated for SHG effectiveness in Jurkat cells.

71 I from bovine Achilles tendon was imaged for SHG in the backscattered geometry and its first-order ef

72 ifically, the measured scattering length for SHG was in excellent agreement with the value predicted

73 ssion based on an electrooptic mechanism for SHG, which depends on the complex-valued first- and seco

74 However, in the case of forward SHG, although the same changes impact upon absolute inte

75 The agreement between Ka values derived from SHG measurements of the interactions between SERMs and a

76 lates well with the mean fiber diameter from SHG images (R(2) = 0.95).

77 and fiber diameter, which are estimated from SHG images using ICS.

78 8% +/- 0.8% to 18.0% +/- 1.3% (measured from SHG images), whereas the storage modulus G' and loss mod

79 taken into account when applying functional SHG imaging as a diagnostic readout for pathological mus

80 agen and myosin by ratiometric epi-generated SHG images at 920 nm and 860 nm.

81 difference between forward and epi-generated SHG provides an explanation for many of the wavelength-d

82 op a model for the forward and epi-generated SHG wavelength-dependence.

83 n the wavelength-dependency of epi-generated SHG.

84 ation of somatic action potentials generated SHG signals at spines with similar amplitude and kinetic

85 Backscattered second harmonic-generated (SHG) NLO signals from these sections were collected as a

86 nfocal imaging of second harmonic-generated (SHG) signals can detect corneal collagen organization.

87 demonstrate that second harmonic generating (SHG) nanoprobes can be used for in vivo imaging, circumv

88 ently introduced second harmonic generating (SHG) nanoprobes.

89 Bright second harmonic generation (SHG) (up to 18 million counts/s) was observed from even

90 tric material is second harmonic generation (SHG) active at both 1064 and 532 nm, with efficiencies o

91 s labeled with a second-harmonic generation (SHG) active dye to supported lipid bilayers.

92 c material shows second-harmonic generation (SHG) activity at both 1064 and 532 nm with efficiencies

93 for quantifying second harmonic generation (SHG) activity of powders that largely decouples linear a

94 each detector in second harmonic generation (SHG) and three parameters for the transmittance of the i

95 myocardium using second harmonic generation (SHG) and two photon excited autofluorescence.

96 dalities such as second-harmonic generation (SHG) and two-photon excited fluorescence (TPEF) have onl

97 drogels produces second harmonic generation (SHG) and two-photon fluorescence (TPF) images, which can

98 through imaging second harmonic generation (SHG) and two-photon fluorescence in engineered and real

99 ) exhibits second-order harmonic generation (SHG) at both 1064 and 532 nm incident radiation with eff

100 Second-harmonic generation (SHG) by membrane-incorporated probes is a nonlinear opti

101 cence (TPEF) and second harmonic generation (SHG) can image the endogenous signals of tissue structur

102 , a custom-built second harmonic generation (SHG) confocal microscope was used to study dynamic sarco

103 n is paid to the second harmonic generation (SHG) effect.

104 violet by direct second harmonic generation (SHG) enabled by a new beryllium-free zincoborate-phospha

105 of epi-generated second harmonic generation (SHG) excitation efficiency, and discriminate collagen an

106 Initial second harmonic generation (SHG) experiments showed crystalline K(4)GeP(4)Se(12) out

107 characterize AF, second harmonic generation (SHG) for collagen, and eosin-labeled fluorescence identi

108 cence (TPEF) and second-harmonic generation (SHG) from biological and inorganic media.

109 Second harmonic generation (SHG) from membrane-bound chromophores can be used to ima

110 sonance enhanced second harmonic generation (SHG) has been used to identify solvation mechanisms at d

111 Second-harmonic generation (SHG) has proven essential for the highest-resolution opt

112 grated use of 3D second harmonic generation (SHG) imaging microscopy and Monte Carlo simulation as a

113 Here we use second harmonic generation (SHG) imaging microscopy to probe structural differences

114 high-resolution second harmonic generation (SHG) imaging of biological tissues and demonstrate its u

115 We performed second harmonic generation (SHG) imaging of collagen in rat-tendon cryosections, usi

116 irectly, we used second harmonic generation (SHG) imaging of membrane potential in pyramidal neurons

117 hich is based on second-harmonic generation (SHG) imaging, enabled detection of homochiral microcryst

118 fluorescence and second harmonic generation (SHG) imaging.

119 Second Harmonic Generation (SHG) is a label-free imaging method used to monitor coll

120 Second harmonic generation (SHG) is an inherently surface specific technique making

121 enhanced surface second harmonic generation (SHG) measurements to track the interaction of the EPA pr

122 mpounds includes second harmonic generation (SHG) measurements, theoretical calculations, infrared an

123 ry properties of second harmonic generation (SHG) microscopy enabled sensitive and selective imaging

124 ere imaged using second-harmonic generation (SHG) microscopy for both collagen and elastin.

125 Second-harmonic generation (SHG) microscopy has emerged as a powerful modality for i

126 y observed in 3D Second Harmonic Generation (SHG) microscopy image data of normal (1) and high risk (

127 Second-harmonic generation (SHG) microscopy is a valuable imaging technique to probe

128 Second harmonic generation (SHG) microscopy measurements indicate that inkjet-printe

129 py combined with second-harmonic generation (SHG) microscopy to selectively detect ChC.

130 interferometric second harmonic generation (SHG) microscopy with femtosecond pulses.

131 rast provided by second harmonic generation (SHG) microscopy, it is possible to identify early molecu

132 trate the use of second harmonic generation (SHG) microscopy-guided synchrotron powder X-ray diffract

133 Red staining and Second Harmonic Generation (SHG) Microscopy.

134 visualizable by second harmonic generation (SHG) microscopy.

135 can be used for second harmonic generation (SHG) microscopy; an incident light of wavelength 840 nm

136 Second-harmonic generation (SHG) originates from the interaction between upconverted

137 luminescence and second-harmonic generation (SHG) phenomena; these have been covered in numerous prev

138 imaging based on second harmonic generation (SHG) provides rapid and highly selective detection of in

139 , exhibit strong second harmonic generation (SHG) response in both crystal and glassy forms.

140 ar optical (NLO) second harmonic generation (SHG) response in the wavelength range of 600-950 nm.

141 exhibits a large second harmonic generation (SHG) response of 13.5 x KDP (600 x alpha-SiO2), and the

142 ay a spontaneous second harmonic generation (SHG) response without any need for preprocessing, and th

143 ar optical (NLO) second harmonic generation (SHG) response.

144 ncy and a strong second harmonic generation (SHG) response.

145 the creation of second harmonic generation (SHG) signals makes it an attractive technique for visual

146 ve in generating second harmonic generation (SHG) signals when adsorbed onto surfaces of colloidal mi

147 ization-resolved second harmonic generation (SHG) spectroscopy at the single-particle level.

148 mental thin-film second harmonic generation (SHG) spectroscopy confirms these trends in calculated re

149 ribe resonant UV second harmonic generation (SHG) studies of the strongly chaotropic thiocyanate ion

150 he properties of second-harmonic generation (SHG) tissue imaging for the functional biological unit f

151 ng time-resolved second-harmonic generation (SHG) to study a ferroelectric (FE)/ferromagnet (FM) oxid

152 d measurement by second-harmonic generation (SHG) together with the anisotropic-bond model of nonline

153 ring (QCM-D) and second harmonic generation (SHG) using solid-supported lipopolysaccharide-containing

154 Second harmonic generation (SHG) was integrated with Raman spectroscopy for the anal

155 In this study, second-harmonic generation (SHG) was used to study RNA and DNA oligonucleotide confo

156 ration (SFG) and second harmonic generation (SHG) were observed from helical fibrils in spinal cord w

157 SONICC relies on second harmonic generation (SHG), a nonlinear optical effect that only arises from n

158 = 1.369 mum) for second harmonic generation (SHG), the largest NLO susceptibility reported to date fo

159 cence (2PAF) and second harmonic generation (SHG), were used to obtain images of the trabecular meshw

160 ential sensitive second harmonic generation (SHG), which is a label-free and interface-selective meth

161 ree technique of second harmonic generation (SHG).

162 e was studied by second harmonic generation (SHG).

163 by measuring the second harmonic generation (SHG).

164 fluorescence and second-harmonic-generation (SHG) imaging.

165 (TPE) and Second Harmonic signal Generation (SHG).

166 and a highly branched, soluble heteroglycan (SHG) are excellent substrates for DPE2 and propose that

167 ed graphitic sheets with stereoscopic holes (SHG) act as effective tri-functional catalysts for the o

168 We discuss how SHG can be used to obtain more structural information on

169 we observed an instantaneous drop of ~50% in SHG signal from the anodic pole of the cell.

170 While image analysis of collagen in SHG images was consistently addressed until now, cellula

171 e aim to take advantage of the difference in SHG between aligned and partially aligned molecules to p

172 We hypothesize that this loss in SHG signal is due to disruption in the interfacial natur

173 e route to metabolize the glucan residues in SHG exists.

174 ons and larger M cations result in increased SHG efficiencies.

175 2-keV (1.033-A) X-rays resulted in increased SHG in the region extending approximately 3 mum beyond t

176 Collagen shows increased SHG intensity at 920 nm, while little difference is dete

177 indicating a possible electric-field-induced SHG (EFISH) mechanism for generating the observed signal

178 The X-ray-induced SHG activity was observed with no measurable loss for lo

179 gth in the backscattered geometry (isotropic SHG component), whereas the spectral profile was consist

180 Finally, an examination of the known SHG active AMCO3F (A = alkali metal, M = alkaline earth

181 he C-site is critical for generating a large SHG response.

182 Ba3(ZnB5O10)PO4, exhibits large SHG responses at 1064 and 532 nm and a short 180 nm abso

183 is article, we explore the use of lipophilic SHG probes to detect minute perturbations in the plasma

184 isson-Boltzmann equation to the TAT liposome SHG data, was shown to be in good agreement with an appr

185 nized noncentrosymmetric structure emits low SHG signal intensity if it has no predominant local pola

186 the same fibril, we concluded that the main SHG signal directly originates from the fibrils, but not

187 K(4)GeP(4)Se(12) also exhibits a measurable SHG response with no poling.

188 maged by conventional two-photon microscopy, SHG nanoprobes appear to generate a signal with an inver

189 e also show that ChCs exhibit a nonvanishing SHG signal, corroborating the noncentrosymmetry of the c

190 low colocalized with SHG (Mcoeff=1), but not SHG signal-voids.

191 effects, collectively that form the observed SHG contrast.

192 te of the art and the physical background of SHG microscopy, and then describe the optical modificati

193 e nanoscale governs the coherent building of SHG signal within the focal volume and is a key advance

194 rtifacts arise due to the phase coherence of SHG signal generation and the Guoy phase shift of the la

195 Intrathecal injection of a combination of SHG and morphine resulted in significantly reduced hind

196 The voltage dependence of SHG by four different probes, three retinoids (all-trans

197 The resulting contrast and detectability of SHG nanoprobes provide unique advantages for molecular i

198 concept, we imaged the phase distribution of SHG signal from the complex collagen architecture of juv

199 mplexity and often inefficient excitation of SHG and TPEF signals.

200 Our findings demonstrate the feasibility of SHG endomicroscopy technology for staging normal pregnan

201 acent to the iris and cornea that is free of SHG signal and consistent with the location of Schlemm's

202 ge zebrafish embryos, and in vivo imaging of SHG nanoprobes during gastrulation and segmentation.

203 Imaging of SHG signals in vehicle-treated eyes showed an anterior l

204 Imaging of SHG signals provides a sensitive means for detection of

205 The detection limit of SHG was estimated to be 4 ppm crystallinity in the powde

206 Making use of the coherent nature of SHG, we monitored in real-time the transfer of 2 and 3 b

207 te understanding of the structural origin of SHG signals from tissues.

208 Our work demonstrates the broad potential of SHG for studying oligonucleotides and their conformation

209 Linear scaling of SHG response with film thickness is observed for benzoic

210 bution of second-order nonlinear emitters on SHG-AIP is highlighted.

211 eration of either bulk optical parameters or SHG alone.

212 minance of primary filter affects on overall SHG generation and attenuation.

213 tion-dependent second-harmonic generation (P-SHG) microscopy.

214 From the P-SHG parameters of vertebrae tissue, a three-dimensional

215 for the preparation and use of a particular SHG nanoprobe label, barium titanate (BT), for in vivo i

216 SHG angular intensity pattern (SHG-AIP) of healthy and proteolysed muscle tissues are s

217 goal, we implemented a circular polarization SHG imaging approach and used it to quantify the intensi

218 Unlike commonly used fluorescent probes, SHG nanoprobes neither bleach nor blink, and the signal

219 s revealed by various validation procedures, SHG signal intensities are a reliable relative measure o

220 It produces SHG intensity similar to that produced by KH(2)PO(4) and

221 (B(SHG)) alone, as only backward-propagating SHG is accessible for true in vivo applications.

222 Quantitative SHG microscopy enabled discrimination of crystal form on

223 This renders SHG microscopy highly sensitive to molecular distributio

224 A comparison with polarization-resolved SHG highlights the crucial role of relative fibril polar

225 ntal implementation of polarization-resolved SHG microscopy combined with mechanical assays, to simul

226 nce that anisotropy of polarization-resolved SHG mostly reflects the micrometer-scale disorder in the

227 4 M and higher concentrations, the resonant SHG signal increases discontinuously, indicating a struc

228 o the structural state of muscle sarcomeres, SHG functional imaging can give insight into the integri

229 nickel(II) porphyrin complexes give similar SHG signals to those of the free-base porphyrins, while

230 ransparency window and achieving the sizable SHG response.

231 ds before being converted to the more stable SHG-inactive polymorphic forms.

232 These can be performed in any standard SHG-microscope that allows polarization control of the i

233 h- and low-intensity regions in the standard SHG images.

234 that gamma-NaAsSe(2) has the highest static SHG coefficient known to date, 337.9 pm/V, among materia

235 In addition to a strong SHG activity, the trimer also shows a strong third harmo

236 (250 nm) of reported materials with a strong SHG response (>10 x KDP).

237 Di-4 proved superior with both strong SHG signal and limited bleaching artifacts.

238 bit is up to approximately 15 times stronger SHG than that of commercially used AgGaSe(2).

239 nates from the fibrils, but not from surface SHG effects.

240 We conclude that SHG and TPF can characterize differential microscopic fe

241 fibrillary acidic protein demonstrated that SHG arises from astroglial filaments.

242 Given that SHG signal is sensitive to the structural state of muscl

243 Our results show that SHG combined with molecular design and synthesis of surf

244 The results show that SHG imaging has great potential as a tool for measuring

245 We also show that SHG-AIP provides information on the three-dimensional st

246 We determined that "SHG vernier" patterns, regions of bifurcated SHG intensi

247 The SHG intensity and forward-backward ratios decrease with

248 The SHG response is type-I phase-matchable and in the wavele

249 The SHG response to voltage was linear and seemed based on a

250 The SHG sensitivity of the chromophore in spines was similar

251 The SHG spectral response of collagen type I from bovine Ach

252 ble substrate affixed to a stretcher and the SHG live-cell imaging technique are unique tools for rea

253 Collectively, the SHG measurements and analysis all indicate that incorpor

254 relative fibril polarity in determining the SHG signal intensity.

255 otein (GFP) prepared in 500 pL droplets, the SHG intensities rivaled those of fluorescence, but with

256 oped the pixel-based approach to extract the SHG signal polarization anisotropy from the same polariz

257 he biophysical mechanism responsible for the SHG voltage sensitivity of the styryl dye FM 4-64 in pyr

258 However, the SHG measurements demonstrated three decade improvements

259 s the mean TPF signal (without impacting the SHG signal) and the storage modulus (16 +/- 3.5 Pa befor

260 We observe a decrease in the SHG anisotropy parameter when the tendon is stretched in

261 ssues lead to significant differences in the SHG depth-dependent directionality and signal attenuatio

262 gen and glucose deprivation, a change in the SHG response to the polarization was measured.

263 structures, we show that the changes in the SHG signals upon ligand binding are the result of struct

264 It is the SHG sensitivity to the electrostatic field generated by

265 ith SHG signal-voids (Mcoeff=1), but not the SHG signal.

266 Mcoeff=1) with SHG signal-voids, but not the SHG signal.

267 , these data illustrate the potential of the SHG approach for detecting and measuring protein conform

268 Monte Carlo simulations of the SHG axial directional and attenuation responses allow th

269 s further supported by colocalization of the SHG contrast with TPEF signals from astrocyte processes

270 , this approach allows for estimation of the SHG creation attributes (directionality and relative con

271 ntified a set of parameters comprised of the SHG creation attributes and the bulk optical parameters,

272 timate that the setup and calibration of the SHG instrument from its component parts will require 2-4

273 Additionally, histograms of the SHG intensities as functions of particle size and orient

274 Functionalization of the SHG nanoprobes takes approximately 3 d, whereas zebrafis

275 Moreover, due to the quasi-coherence of the SHG process in tissues, we submit that this approach con

276 by measuring the intensity modulation of the SHG signal as a function of the angular rotation of the

277 demonstrate that different magnitudes of the SHG signal changes are due to different and specific lig

278 Further polarization analysis of the SHG signal showed that the symmetry property of the fibr

279 Colocalization of the SHG signal with two-photon excitation fluorescence (TPEF

280 ential do not affect the polarization of the SHG signal.

281 n resulted in the gradual destruction of the SHG signal.

282 amorphous vitreous solvents, analysis of the SHG spatial profiles following X-ray microbeam exposure

283 NLO-active structural units in producing the SHG responses.

284 l's resting potential, the voltage where the SHG is minimal, and the amplitude of the signal at that

285 focus of a laser-scanning microscope, these SHG nanocrystals convert two photons into one photon of

286 without any need for preprocessing, and this SHG activity appears to be stable over several months.

287 n the other hand can potentially be added to SHG and TPEF to visualize a much broader range of marker

288 with resolution similar to that of bench-top SHG microscopy.

289 Here, we demonstrate single-trial SHG recordings of neuronal somatic action potentials and

290 ect of the N, S heteroatom doping and unique SHG architecture, which provide a large surface area and

291 in membrane symmetry could be detected using SHG, we exposed cells to nanosecond-pulsed electric fiel

292 lagen type I arrays that can be imaged using SHG microscopy.

293 ively probe the ferroelectric response using SHG.

294 For intact ex vivo SHG imaging of Achilles tendon, we observe a significant

295 to be a signature of healthy muscle whereas SHG-AIP with one centered spot in pathological mdx muscl

296 purpose of this study was to assess whether SHG signals can detect differences in corneal fibrosis a

297 d quantitatively colocalized (Mcoeff=1) with SHG signal-voids, but not the SHG signal.

298 and quantitatively, AF-low colocalized with SHG (Mcoeff=1), but not SHG signal-voids.

299 AF-high colocalized with SHG signal-voids (Mcoeff=1), but not the SHG signal.

300 ne materials are type-I phase-matchable with SHG coefficients chi((2)) of 151.3 and 149.4 pm V(-1) fo