ライフサイエンスコーパス: SICM

1 SICM can be used to analyze cell morphology at nanoscale

2 In addition, SICM has been integrated with a range of other technique

3 d carbon electrode for pH measurement and an SICM barrel for distance control, enabling simultaneous

4 etermining the tip-sample distance during an SICM experiment.

5 . C 20.60+/- 3.174x10(5), n=56; P<0.001) and SICM studies revealed a profound disruption to the openi

6 The fabricated probes, with pH and SICM sensing elements typically on the 100 nm scale, wer

7 our new, to our knowledge, angular approach SICM allows imaging of living cells on nontransparent su

8 Using this angular approach SICM, we obtained topographical images of cells grown on

9 ue to the limitations of currently available SICM systems that inherited their design from other scan

10 red scanning ion conductance microscopy (bio-SICM) approach that couples the imaging ability of SICM

11 urther optimization, we believe that the bio-SICM platform will provide a powerful analytical methodo

12 To establish the framework of the bio-SICM platform, we utilize the well-studied protein chann

13 Bias modulated (BM)-SICM is compared to conventional SICM imaging through me

14 Finally, BM-SICM with both amplitude and phase feedback is used for

15 In conclusion, SICM, unlike other techniques, can reliably deliver prec

16 ted ultramicroelectrode (UME) for concurrent SICM and scanning electrochemical microscopy (SECM).

17 feedback signal, as needed for conventional SICM modes.

18 ulated (BM)-SICM is compared to conventional SICM imaging through measurements of substrates with dis

19 ferential concentration mode of SICM (DeltaC-SICM) also enhances surface charge measurements and prov

20 ce on the nanopipette response in the DeltaC-SICM configuration compared to standard SICM modes.

21 we demonstrate a new method for determining SICM tip geometry that overcomes the limitations of EM i

22 For SICM imaging, the assumption is made that positions of e

23 electrode as part of a conductance cell for SICM.

24 However, SICM imaging remains insensitive to electrochemical prop

25 y for substantially better quantification in SICM imaging and measurement.

26 et in a scanning ion conductance microscope (SICM) can exert localized forces on a sample surface.

27 The scanning ion conductance microscope (SICM) is a powerful tool for imaging the topography of s

28 The scanning ion conductance microscope (SICM) is an emerging tool for noncontact topography imag

29 own the scanning ion conductance microscope (SICM) to be a very promising tool to spatially resolve a

30 ectrode scanning ion conductance microscope (SICM).

31 ng with scanning ion conductance microscopy (SICM) and other scanning probe microscopies.

32 taneous scanning ion conductance microscopy (SICM) and scanning electrochemical microscopy (SECM) mea

33 olution scanning ion conductance microscopy (SICM) extends the utility of SICM by enabling selective

34 using a scanning ion conductance microscopy (SICM) format.

35 Scanning ion conductance microscopy (SICM) has developed into a powerful tool for imaging a r

36 Scanning ion conductance microscopy (SICM) is a nanopipette-based scanning probe microscopy t

37 Scanning ion conductance microscopy (SICM) is a powerful technique for imaging the topography

38 Scanning ion conductance microscopy (SICM) is a scanned probe microscopy technique in which t

39 Scanning ion conductance microscopy (SICM) is a scanning probe technique that allows investig

40 Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that

41 Scanning ion conductance microscopy (SICM) is demonstrated to be a powerful technique for qua

42 nvasive scanning ion conductance microscopy (SICM) of cells and which must be overcome in order to fo

43 Scanning ion conductance microscopy (SICM) offers the ability to obtain very high-resolution

44 Scanning ion conductance microscopy (SICM) offers the ability to perform contact-free, high-r

45 tion pH-scanning ion conductance microscopy (SICM) probes is reported.

46 a novel scanning ion conductance microscopy (SICM) technique for assessing the volume of living cells

47 used in scanning ion conductance microscopy (SICM) to determine, in a noncontact manner, the topograp

48 Scanning ion conductance microscopy (SICM) was used to interrogate ion currents emanating fro

49 With scanning ion conductance microscopy (SICM), a noncontact scanning probe technique, it is poss

50 py, and scanning ion conductance microscopy (SICM).

51 Using distance-modulated SICM, which induces an alternating ion current component

52 urthermore, the use of a distance modulation SICM scheme allows reasonably faithful probe positioning

53 we describe the designs that allow mounting SICM scan head on a standard patch-clamp micromanipulato

54 approach that couples the imaging ability of SICM with the sensitivity and chemical selectivity of pr

55 One of the great advantages of SICM lies in its ability to perform contact-free scannin

56 The application of SICM reaction imaging is demonstrated on several example

57 y also provide a framework for the design of SICM experiments, which may be convoluted by topographic

58 ontributes significantly to the emergence of SICM as a multifunctional technique for simultaneously p

59 This differential concentration mode of SICM (DeltaC-SICM) also enhances surface charge measurem

60 k for understanding the contact-free mode of SICM and also extend previous findings with regard to SI

61 Despite the increasing popularity of SICM, several aspects of the imaging process are still u

62 this report, we develop the use and scope of SICM, showing how it can be used for mapping spatial dis

63 been undertaken to enhance understanding of SICM as an electrochemical cell and to enable the interp

64 nce microscopy (SICM) extends the utility of SICM by enabling selective chemical imaging of specific

65 annel capillary probes consisting of an open SICM barrel, and a solid carbon SECM electrode enabled c

66 P-SICM shows the ability to differentiate transport throug

67 ies conferred by claudin-2 are captured by P-SICM which demonstrates the utility to monitor apparent

68 We describe P-SICM investigations of both wild type and tricellulin ov

69 etric-scanning ion conductance microscopy (P-SICM) for ion-conductance measurement in polymer membran

70 etric scanning ion conductance microscopy (P-SICM), that utilizes a nanoscale pipet to differentiate

71 etric scanning ion conductance microscopy (P-SICM).

72 e, we combine hopping mode techniques with P-SICM to allow simultaneous nanometer-scale conductance a

73 em illustrates the quantitative nature of pH-SICM imaging, because the dissolution process changes th

74 The capability of the pH-SICM probe was demonstrated by detecting both pH and top

75 These pH-SICM probes were fabricated rapidly from laser pulled th

76 and it provides a framework for quantitative SICM studies.

77 The suitability of the probes for SECM-SICM imaging is demonstrated by both feedback-mode and s

78 cal-scanning ion conductance microcopy (SECM-SICM) has been used to map the electroactivity of surfac

79 monstrates the value of high-resolution SECM-SICM for low-current amperometric imaging of nanosystems

80 This probe geometry enables successful SECM-SICM imaging on features as small as 180 nm in size.

81 ltaC-SICM configuration compared to standard SICM modes.

82 The SICM method can also be used for rapid estimation of the

83 si-reference counter electrode (QRCE) in the SICM nanopipet probe and a second QRCE in the bulk solut

84 molecules highly tunable via control of the SICM bias to promote or restrict migration from the pipe

85 n is highly dependent on the geometry of the SICM probe, which is generally not known.

86 ts have allowed a critical assessment of the SICM response as a means of probing surface topography.

87 l experimental practice, the response of the SICM tip to surface features occurs over much greater la

88 Despite the rising popularity of the SICM, the image formation process and the fundamental li

89 ngs by changing the applied voltage over the SICM nanopipette.

90 The data can be routinely obtained using the SICM apparatus itself and our method thus opens the way

91 Practical implementations of these SICM advantages, however, are often complicated due to t

92 also extend previous findings with regard to SICM resolution.

93 nstrate the utility of this understanding to SICM by topographically mapping a live cell's cytoskelet

94 topography scans of the cardiomyocytes using SICM and then determined the electrophoretic mobility of

95 le transport properties can be measured with SICM.

96 r mapping surface charge and topography with SICM, which increases the data acquisition rate by an or