ライフサイエンスコーパス: SL RNA

1 SL RNA genes are individually transcribed from the only

2 t decreased in vitro eEF1A binding to the 3' SL RNA also decreased viral minus-strand RNA synthesis i

3 ed the efficiency of eEF1A binding to the 3' SL RNA increased minus-strand RNA synthesis in transfect

4 The data suggest that interaction of the 3' SL RNA with eEF1A at three sites and a unique metastable

5 n reduced the binding activity of the WNV 3' SL RNA for EF-1 alpha by approximately 60%.

6 he interaction between EF-1 alpha and WNV 3' SL RNA was calculated to be 1.1 x 10(-9) M.

7 he interaction between EF-1 alpha and WNV 3' SL RNA was specific.

8 extracts and in vitro-synthesized WNV (-)3' SL RNA as the probe.

9 cted in BHK cell extracts with the WNV (-)3' SL RNA were found in both C3H/RV and C3H/He cell extract

10 by UV-induced cross-linking to the WNV (-)3' SL RNA.

11 WNV 3' minus-strand stem-loop RNA [WNV (-)3' SL RNA] confirmed the existence of a terminal secondary

12 EF-1 alpha binds to each molecule of WNV 3' SL RNA.

13 binding site on the main stem of the WNV 3' SL RNA.

14 d that specifically interact with the WNV 3' SL RNA.

15 phosphatase inhibited its binding to WNV 3' SL RNA.

16 unoprecipitated both the RV 5'(+)SL and 3'(+)SL RNA-protein complexes.

17 Autoantigens binding the RV 5'(+)SL and 3'(+)SL RNAs differed in molecular mass, specificities for re

18 eraction between TIAR RRM2 and the WNV 3'(-) SL RNA was 1.5 x 10(-8), while that for TIA-1 RRM2 was 1

19 lasmic extracts incubated with the WNV 3'(-) SL RNA was immunoprecipitated by anti-TIAR antibody.

20 The binding site for the WNV 3'(-) SL RNA was mapped to RRM2 on both TIAR and TIA-1.

21 TIA-1 also binds to the WNV 3'(-) SL RNA.

22 e West Nile virus minus-stand RNA [WNV 3'(-) SL RNA].

23 action between TIAR/TIA-1 and the viral 3'(-)SL RNA is not required for initial low-level symmetric R

24 lular RNA-binding protein binds to the RV 5' SL RNA with sufficient specificity for the binding to oc

25 tigen was found to interact with the RV 5'(+)SL RNA as determined by immunological techniques and bin

26 cellular RNA, with significant homology to 7 SL RNA is associated with Puralpha.

27 We report the characterization of an SL RNA processing complex with TbMTr1 and the SLA1 H/ACA

28 To analyze SL RNA genesis, we mutated SL RNA intron structures and

29 ere indispensable for parasite viability and SL RNA gene transcription.

30 We conclude that TbCgm1 specifically cap SL RNA, and cap 0 is a prerequisite for subsequent methy

31 ccumulating species in SNIP knockdown cells, SL RNA 3'-end formation is a multistep process in which

32 rypanosomes, we have begun by characterizing SL RNA gene expression in the model trypanosome Leptomon

33 hom human African trypanosomiasis was cured, SL RNA detection had specificities of 98.4%-100%, while

34 TAP-null lines are viable but have decreased SL RNA processing efficiency in cap methylation, 3'-end

35 In dinoflagellates, SL RNAs are unusually short at 50-60 nt, with a conserve

36 ith an m(2,2,7)G-cap and a common downstream SL RNA sequence.

37 During SL RNA transcription, accurate initiation is determined

38 Accumulation of 3'-extended SL RNA substrate indicates a delay in processing and sug

39 ription factor IIB (tTFIIB) is essential for SL RNA gene transcription and cell viability, but has a

40 ence element and is absolutely essential for SL RNA gene transcription in vitro.

41 associates with tSNAPc and is essential for SL RNA gene transcription.

42 nosoma cruzi (CL Brener) genome projects for SL RNA repeat sequences in order to assess their homogen

43 onexosomal, 3'-->5' exonuclease required for SL RNA 3'-end formation in Trypanosoma brucei.

44 splicing, in which a capped SL, derived from SL RNA, is spliced onto the 5' end of each mRNA.

45 The spliced leader RNA gene (SL RNA) repeat is present in large multicopy arrays and

46 d and are predicted to yield a heterogeneous SL RNA population.

47 predicted secondary structures of the Hydra SL RNAs show significant differences from the structures

48 A and lead to accumulation of hypomethylated SL RNA.

49 ption initiates at the +1 of the interrupted SL RNA gene and proceeds into the 5' UTR and open readin

50 is common to the 3' end of all kinetoplastid SL RNA genes, and that more than six T's are required fo

51 ma brucei, the small nuclear spliced leader (SL) RNA and the large rRNAs are key molecules for mRNA m

52 m7G cap is restricted to the spliced leader (SL) RNA and the precursors of U2, U3, and U4 snRNAs.

53 tiation complex (PIC) at the spliced leader (SL) RNA gene (SLRNA) promoter.

54 rms are generated in which a spliced leader (SL) RNA gene is fused with a sequence tag and driven by

55 egrates exclusively into the spliced leader (SL) RNA genes.

56 port the first data that the spliced leader (SL) RNA is a more specific marker for cure of human Afri

57 atids, trans splicing of the spliced leader (SL) RNA is a required step in the maturation of all nucl

58 Spliced leader (SL) RNA trans splicing adds a trimethylguanosine (TMG) c

59 Spliced leader (SL) RNA trans-splicing contributes the 5' termini to mRN

60 ase II-mediated synthesis of spliced leader (SL) RNA, a trans splicing substrate and key molecule in

61 lies on the synthesis of the spliced leader (SL) RNA.

62 zi, and Leishmania spp., the spliced-leader (SL) RNA is a key molecule in gene expression donating it

63 from a short transcript, the spliced-leader (SL) RNA.

64 The two spliced leader (SL) RNAs (SL-A and -B) contain splice donor dinucleotide

65 f cap 4 formation on the 140-nucleotide-long SL RNA.

66 le virus minus-strand 3' terminal stem loop (SL) RNA was previously shown to bind specifically to cel

67 eins associated with the RV 5'(+) stem-loop (SL) RNA are recognized by serum with Ro reactivity.

68 e cell protein eEF1A on the 3'(+) stem-loop (SL) RNA of West Nile virus (WNV) (3).

69 on events leading to the formation of mature SL RNA.

70 To analyze SL RNA genesis, we mutated SL RNA intron structures and sequence elements: stem-loo

71 6 nucleotides result in the appearance of no SL RNA, consistent with the in vitro studies by others s

72 mediator is a basal factor for small nuclear SL RNA gene transcription in trypanosomes and that the b

73 ng domain, human 3'hExo, and a 26-nucleotide SL RNA.

74 y, SPF27 silencing caused an accumulation of SL RNA with a hypomethylated cap that closely resembled

75 Towards understanding the coordination of SL RNA and mRNA expression in trypanosomes, we have begu

76 of one- to two- nucleotide 3' extensions of SL RNA, U2 and U4 snRNAs, a five-nucleotide extension of

77 organisms crucially depend on high levels of SL RNA synthesis.

78 smic machinery responsible for processing of SL RNA.

79 th alpha-amanitin sensitivity reminiscent of SL RNA synthesis and is dependent on the SL RNA promoter

80 ent and nucleoplasmic Sm-dependent stages of SL RNA maturation combined with reduced rates of transla

81 Here, we analyzed the spatial expression of SL RNAs in the planarian flatworm Schmidtea mediterranea

82 4 modification, indicating that SNIP acts on SL RNA after cytosolic trafficking.

83 and TbCMT1 did not affect parasite growth or SL RNA capping.

84 A of uniform sequence (the spliced leader or SL RNA) has allowed us to characterize the RNAs with whi

85 Since the primary SL RNA transcript was not the accumulating species in SN

86 As putative SL RNA gene transcription factors, a partially character

87 The role of spliced leader RNA (SL RNA) in trans-splicing in Caenorhabditis elegans has

88 gm1 transfers the GMP to spliced leader RNA (SL RNA) via a covalent enzyme-GMP intermediate, and meth

89 g-specific approximately 100-nucleotide RNA (SL RNA) that bears striking similarities to the cis-spli

90 sfer of a short leader sequence from a small SL RNA to the 5' end of a subset of pre-mRNAs.

91 rified and characterized a sequence-specific SL RNA promoter-binding complex, tSNAP(c), from the path

92 localized to the nucleoplasm, and substrate SL RNA derived from SNIP knockdown cells showed wild-typ

93 '-O-ribose methylation at cap 1 on substrate SL RNA and U1 small nuclear RNA.

94 TbMTr2-/-/TbMTr3-/- lines yielded substrate SL RNA and mRNA with cap 1.

95 Using an exon-tagged SL RNA gene, we examined the phenotypes produced by a se

96 Only in the tissue of interest, the tagged SL RNA gene is transcribed and then trans-spliced onto m

97 with similar efficiencies to the 3'-terminal SL RNAs of four divergent flaviviruses, including a tick

98 could be detected on prematurely terminated SL RNA transcripts of 56 nucleotides in length and longe

99 ta from our proof-of-concept study show that SL RNA detection has high potential as a test of cure.

100 The in vivo and in vitro data suggest that SL RNA transcription termination is staggered in the T t

101 The SL RNA cap structure in Trypanosoma brucei is unique amo

102 The SL RNA gene (SLRNA) promoter recruits RNA polymerase II

103 The SL RNA gene in Leptomonas seymouri is a member of the sm

104 The SL RNA gene promoter has been shown by in vitro and in v

105 The SL RNA genes are expressed from the only defined RNA pol

106 The SL RNA in Leishmania tarentolae is a 96-nt precursor tra

107 The SL RNA is a small nuclear RNA and a trans splicing subst

108 The SL RNA is present in the cell in the form of a ribonucle

109 The SL RNA is transcribed with an oligo-U 3' extension that

110 f vertebrate small nuclear RNA genes and the SL RNA homologue in Ascaris.

111 by eIF4E/G interaction with the cap and the SL RNA, although the SL does not increase the affinity o

112 f the most abundant crosslinks formed by the SL RNA allowed us previously to identify the spliced lea

113 ear RNAs U1, U2, U4 and U5; for example, the SL RNA functions only if it is assembled into an Sm smal

114 he transcription termination element for the SL RNA gene.

115 However, the SL RNA is required in stoichiometric amounts for trans-s

116 bsequent modifications characteristic of the SL RNA cap 4 were added successively in a 5' to 3' direc

117 m(7)G capping of the SL RNA could be detected on prematurely terminated SL RN

118 Expression of the SL RNA gene requires sequence elements at bp -60 to -70

119 w here that the 5' splice site region of the SL RNA is also crosslinked in vivo to a second small RNA

120 Expression of the SL RNA is initiated at the only known RNA polymerase II

121 e capped spliced leader (SL) sequence of the SL RNA onto the 5' end of all mRNAs.

122 n, that was caused by hypomethylation of the SL RNA's unique cap4.

123 of SL RNA synthesis and is dependent on the SL RNA promoter.

124 processing of multiple modifications on the SL RNA substrate.

125 sm for generating the cap 4 structure on the SL RNA.

126 ence, which is derived from a small RNA, the SL RNA, to all mRNA precursors.

127 ystem, we demonstrate in this study that the SL RNA is transcribed by RNA polymerase II.

128 P(c)/TFIIA complex binds specifically to the SL RNA gene promoter upstream sequence element and is ab

129 ipate in recruiting RNA polymerase II to the SL RNA gene promoter.

130 13, 12, and 8 kDa were co-selected with the SL RNA from Leptomonas collosoma, representing the SL RN

131 initiation complex that assembles within the SL RNA gene promoter.

132 s robust transcriptional activity within the SL RNA gene.

133 Both of the SL RNAs are bound by antibody against trimethylguanosine

134 1 and SL2, S. mediterranea expresses a third SL RNA described here as SL3.

135 lex enables efficient translation of the TMG-SL RNAs in diverse in vitro translation systems.

136 In SL trans-splicing, SL-donor transcripts (SL RNAs) contain two functional domains: an exon that pr

137 t and fourth (m(7)Gpppm(6,6)AmpAmpCmpm(3)Ump-SL RNA) conveyed via trans-splicing of a universal splic

138 rference increased the abundance of uncapped SL RNA and lead to accumulation of hypomethylated SL RNA

139 s, including Caenorhabditis elegans, utilize SL RNA in their mRNA maturation programs.

140 We describe mutants whose SL RNAs end in the T tract or appear to lack efficient t

141 the present study, the sites on the WNV3'(-)SL RNA required for efficient in vitro T-cell intracellu

142 located in two internal loops of the WNV3'(-)SL RNA structure.

143 ile virus 3' minus-strand stem-loop [WNV3'(-)SL] RNA (37) and colocalized with flavivirus replication