ライフサイエンスコーパス: SP cell

1 and homogeneous population of cells (termed SP cells).

2 populations and accelerated transition into SP cells.

3 on program and developmental maturation into SP cells.

4 that are either salient or depressed in the SP cells.

5 ified nine pathways that were altered in the SP cells.

6 nificantly different between MCF7 SP and non-SP cells.

7 s region-dependent passive dispersion of the SP cells.

8 colonies derived from retrovirus-transduced SP cells.

9 ng cardiomyogenic differentiation of cardiac SP cells.

10 munophenotypically distinct from bone marrow SP cells.

11 e ERalpha, p21(CIP1) and Msi1 genes than non-SP cells.

12 hey were phenotypically distinct from marrow SP cells.

13 y a minority of antigen-defined subsets were SP cells.

14 t secreted frizzled-related protein 4 in non-SP cells.

15 ntribute to the resistant phenotype of H1650 SP cells.

16 re apparent in CD4 SP thymocytes than in CD8 SP cells.

17 an endothelial marker shown to be absent on SP cells.

18 efflux from leukemic SP cells than from non-SP cells.

19 ymocytes, albeit at lower levels than in CD4 SP cells.

20 nses and expression of IL-2Ralpha on CD8high SP cells.

21 flux the dye in a manner identical to murine SP cells.

22 vels are present primarily on 80- 85% of CD4 SP cells.

23 phenotype of lung tumors derived from H1650 SP cells.

24 eous multi-lineage differentiation of muscle SP cells.

25 lains the enhanced activation of untuned CD4 SP cells.

26 ization, and functional relevance of cardiac SP cells.

27 contrast, non-SP cells did not give rise to SP cells.

28 3alpha/beta, MEK1, c-Jun, p53, and p70S6K in SP cells.

29 aled higher tumorigenicity compared with non-SP cells.

30 ta thymocytes is very similar to that of CD4 SP cells.

31 higher proliferation index compared with non-SP cells.

32 nd significantly decreased the percentage of SP cells.

33 ing modifications in double-positive and CD8 SP cells.

34 e, viability, and proliferation potential of SP cells.

35 s: semilunar (SL) and superficial pyramidal (SP) cells.

36 dyes and is referred to as side population (SP) cells.

37 l, CSCs are enriched in the side population (SP) cells.

38 ched for stem cells, termed side population (SP) cells.

39 ing Hoechst 33342-extruding side population (SP) cells.

40 xpression of the endogenous Str-1 gene in A5(SP) cells.

41 by the more active JunD-Fra-2 complex in A5(SP) cells.

42 hematopoietic cells termed side population (SP) cells.

43 hymocytes were truly mature single-positive (SP) cells.

44 other in mature CD4 or CD8 single-positive (SP) cells.

45 stem-like features known as side population (SP) cells.

46 i NP) were then integrated with Sphingomonas sp. cells.

47 Culture of BM "side population" (SP) cells, a highly enriched stem cell population, for 1

48 t 33342 fluorescence-sorted side population (SP) cells, a subset of bone marrow enriched for stem cel

49 y showed high uptake of CLG-shAnxA2 in H1650 SP cells after 2h resulting in a 6-fold reduction in Anx

50 egy involved the cotransplantation of single SP cells along with different populations of competitor

51 ncreased resistance to chemotherapy, and 4T1 SP cells also showed an increased ability to efflux doxo

52 a model in which information flow from SL to SP cells and back to the OB is mediated by a hierarchica

53 We define the molecular signature of cardiac SP cells and compare it to embryonic stem cells and adul

54 skeletal muscle, but differ from bone marrow SP cells and do not express hematopoietic markers.

55 te the hematopoietic potential of individual SP cells and find substantially lower rates of reconstit

56 for amplification of phenotypically defined SP cells and functionally defined repopulating cells.

57 the molecular signature of the conjunctival SP cells and identify markers and signaling pathways ass

58 , and miR-9 inhibition reduced the number of SP cells and metastasis.

59 e in peripheral blood progeny of bone marrow SP cells and prolonged skin graft survival across this c

60 hairpin markedly enhances development of CD8 SP cells and reduces CD4 SP development.

61 ns of Wnt signaling pathway agonist Wnt3a in SP cells and the antagonist secreted frizzled-related pr

62 Accordingly, SP cells and their tumor-initiating subset were more res

63 he weights of lung tumors derived from H1650 SP cells and tumor burden was reduced to only 19% of con

64 4(+)CD8(-) and CD4(-)CD8(+) single-positive (SP) cells and increased percentage of CD69(low)CD24(low)

65 ecreased the percentage and clonogenicity of SP cells, and also induced phosphorylation changes in Ak

66 ncreased in purified MCF7 SP relative to non-SP cells, and incubation with an ABCG2-specific inhibito

67 ere sorted by flow cytometry into SP and non-SP cells, and purified RNA was processed for microarray

68 age negative, c-Kit-positive, Sca-1-positive SP cells, and the residual SP cells were depleted of rep

69 1) as differentially upregulated in melanoma SP cells, and there was a significant increase of PCDHB1

70 ducing the ratio of CD4/CD8 single-positive (SP) cells; and reducing cell surface CD5 expression.

71 Since most SP cells are a subset of c-kit+, Thy-1+, lineage neg, SC

72 rmore, we determine that CD31-/Sca1+ cardiac SP cells are capable of both biochemical and functional

73 ent studies suggest that transplanted single SP cells are capable of lymphohematopoietic repopulation

74 enes via SP cells is possible and that these SP cells are capable of recapitulating the myogenic line

75 A large fraction of these SP cells are CD44 and CD24 positive, are gemcitabine res

76 nificant number of, but not all, limb muscle SP cells are derived from the hypaxial somite.

77 nirradiated mdx mice, nuclei from donor skin SP cells are found within myofibers that express dystrop

78 Taken together, these findings indicate that SP cells are frequently involved in human AML and may be

79 Given that muscle SP cells are heterogeneous, it has been difficult to pro

80 tro culture studies demonstrated that rhesus SP cells are highly enriched for long-term culture-initi

81 SP cells are identified and characterized by a unique ef

82 Hence, although SP cells are identified independently of antigenic marke

83 Overrepresentation analysis indicated that SP cells are in a low metabolic and biosynthetic state.

84 unctional heterogeneity; somitically derived SP cells are intrinsically more myogenic than nonsomitic

85 ed, the functional roles of WM and postnatal SP cells are not known.

86 SP cells are resistant to paclitaxel because of the upre

87 type with little tumorigenic potency, while SP cells are very similar to triple-negative ones that d

88 ping and clonal in vitro assays we find that SP cells are virtually homogeneous.

89 Side population (SP) cells are enriched for hematopoietic stem cell activ

90 Side population (SP) cells are identified as cells capable of excluding t

91 Muscle side population (SP) cells are rare multipotent stem cells that can parti

92 Muscle side population (SP) cells are rare myogenic progenitors distinct from sa

93 Skeletal muscle side population (SP) cells are thought to be "stem"-like cells.

94 bserved evidence for clonogenic potential of SP cells, as well as the ability of SP cells to regenera

95 Surprisingly, mostly during midgestation, SP cells become secondarily displaced and widespread int

96 provide a useful in vitro system to analyze SP cell biology in both normal and pathological conditio

97 chical feedback circuit, whereas both SL and SP cells broadcast information to higher olfactory areas

98 etic protein 4 (BMP4) is highly expressed in SP cells but not in main population (MP) mononuclear mus

99 colonies derived from lentivirus-transduced SP cells, but in only 4% of colonies derived from retrov

100 Moreover, we show that C6 SP cells, but not non-SP cells, can generate both SP and

101 lls (HSCs) from bone marrow side-population (SP) cells by using a transgenic reporter gene driven by

102 Thus, adult skin SP cells can engraft in dystrophic skeletal muscle even

103 Finally, we provide evidence that C6 SP cells can produce both neurons and glial cells in vit

104 eover, we show that C6 SP cells, but not non-SP cells, can generate both SP and non-SP cells in cultu

105 The engrafted SP cells (CD34(-)/low, c-Kit(+), Sca-1(+)) or their prog

106 Side population (SP) cells, characterized by a distinct Hoechst dye efflu

107 Untuned CD4 SP cells contain a pool of Lck with increased basal phos

108 sitive cells, which account for 20% of total SP cells, contain all the LTR-HSC activity within bone m

109 tions, those with increased side population (SP) cells correlated with epithelial-mesenchymal transit

110 The SP cells could differentiate into non-SP tumor cells, wi

111 They also suggest a mechanism by which SP cells could escape the effects of cytostatic drugs an

112 Using mutant SP cells deficient in glucosidase I, we confirm that the

113 Our data indicate that YS and embryonic SP cells detected prior to the onset of circulation expr

114 onist during positive selection inhibits CD8 SP cell development but is not sufficient to divert clas

115 In contrast, non-SP cells did not give rise to SP cells.

116 The CD7(+)CD34(-)Lin(-) UCB SP cells did not proliferate in simple suspension cultur

117 d, B9(SQ) cells did not make Str-1, while A5(SP) cells did.

118 et al. (2012) report that they have isolated SP cells directly from patients' melanomas.

119 Although rhesus SP cells do not initially possess any hematopoietic colo

120 5 mo) of at least some postselection CD4(+) SP cells do not require the TCR-MHC class II interaction

121 SP cells engrafted into endothelium during vascular rege

122 helium during vascular regeneration, and non-SP cells engrafted into smooth muscle.

123 tumorigenicity in vivo, suggesting that MCF7 SP cells enrich cancer stem-like cells.

124 d an SP in each, and demonstrated that these SP cells exhibit distinct phenotypic and functional char

125 However, SP cells exhibit greater frequency-dependent action pote

126 SP cells exhibit substantial heterogeneity in MM cell li

127 Lymphoma SP cells exhibited autonomous clonogenicity and exported

128 Additionally, 4T1 and NXS2 SP cells exhibited increased resistance to chemotherapy,

129 Further, MCAM+ SP cells exhibited the highest fusion potential in vitro

130 In addition, the SP cells express higher levels of pluripotency-associate

131 Finally, our data show that the SP cells express higher levels of the NPM-ALK oncogene a

132 Approximately half of SP cells expressed CD34 at readily detectable levels, an

133 However, the SP cells failed to differentiate into mature myotubes, a

134 We tested BM-SP cells for their ability to regenerate heart and skele

135 In contrast to non-SP cells, SP cells formed branching structures in matrigel which i

136 In vivo, mouse SP cells formed measurable tumors sooner than non-SP (NS

137 th a selective increase in the percentage of SP cells found in the tumors of doxorubicin-treated mice

138 We used the so-called side population (SP) cells found in the MCF7 breast cancer cell line, whi

139 Hence, the SP cell fraction highlights a subset of the long-term re

140 Here, we report that SP cells from a soft tissue tumor of enigmatic origin te

141 n culture, whereas there was a rapid loss of SP cells from control cultures.

142 This report characterizes SP cells from human umbilical cord blood (UCB).

143 is a previously unreported study to isolate SP cells from melanoma patients and to investigate the g

144 stem cells/progenitors and those enriched as SP cells from mitogen-exposed retinal cell culture may b

145 BM-SP cells from normal male mice were transplanted into fe

146 We and others have identified SP cells from postmitotic tissues, including adult myoca

147 In this study, we characterized SP cells from the human breast cancer cell line MCF7 as

148 wal, and multipotentiality compared with non-SP cells from the same tumors.

149 SP cells to serve as precursors for muscle, SP cells from the two mice strains mdx(5cv) and C57BL/6N

150 SP cells from these tumors displayed an unexpectedly hig

151 Here we identify and characterize SP cells from two distinct genetically engineered mouse

152 Using this approach, we show that SP cells from wild type muscle robustly differentiate in

153 The retinal SP cells generated functional neurons and glia and posse

154 Studies in mice demonstrated that muscle SP cells give rise to satellite cells in vivo.

155 Compared with non-SP cells, MCF7 SP cells had higher colony-formation ability in vitro an

156 Unfortunately, the study of muscle SP cells has been challenged by their low abundance and

157 SOX2 in promoting the resistant phenotype of SP cells has not been investigated.

158 population of cells, termed side population (SP) cells, has stem cell characteristics as they have be

159 These SP cells have enhanced tumor-initiating capacity, self-r

160 reover, we provide evidence that human MCAM+ SP cells have intrinsic myogenic activity that is retain

161 By contrast, proportionately more SP cells have matured, and SP-to-DP ratios are increased

162 roup of cells called muscle side population (SP) cells have been isolated based on their ability to e

163 e in muscle repair, whereas side population (SP) cells have more recently been identified as contribu

164 ouble positive (DP) and CD8 single positive (SP) cells, high levels are present primarily on 80- 85%

165 erived from, and replenished by, bone marrow SP cells; however, within the muscle environment, they w

166 lls to non-SP cancer cells and normal neural SP cells identified 45 candidate genes that are differen

167 the transition from double-positive (DP) to SP cell in a physiological situation.

168 We review the initial discovery of cardiac SP cells in adult myocardium as well as their capacity f

169 centage of CD69(low)CD24(low) and CD62L(low) SP cells in cDKO cells imply retention of SP cells in th

170 o has intriguing implications for the use of SP cells in clinical orthopedics and stem cell-based dis

171 t non-SP cells, can generate both SP and non-SP cells in culture and are largely responsible for the

172 eneous population containing both SP and non-SP cells in culture.

173 e implications for a possible role of muscle SP cells in fibrosis and fat deposition in muscular dyst

174 Here, the prevalence and pathogenic role of SP cells in human AML were investigated.

175 to prospectively enrich for myogenic muscle SP cells in human fetal muscle.

176 s responsible for the efflux of Hoechst from SP cells in our MYC-driven model.

177 to a significant reduction in the number of SP cells in the bone marrow and in skeletal muscle.

178 and platelet-derived growth factor maintains SP cells in the C6 glioma cell line.

179 We used a similar method to identify SP cells in the skin of adult mice.

180 elopment, with a pronounced skew towards CD8 SP cells in the thymus and developed a low incidence of

181 w) SP cells in cDKO cells imply retention of SP cells in the thymus.

182 In this study, we confirmed the presence of SP cells in tumors from melanoma patients.

183 recent identification of "side population" (SP) cells in a number of unrelated human cancers and the

184 e selection to CD4+ or CD8+ single positive (SP) cells in vivo or activation-induced cell death in vi

185 loglucans synthesised by cultured rose (Rosa sp.) cells in "heavy" or "light" media (with [13C,2H]glu

186 ssfully cultured 'Paul's Scarlet' rose (Rosa sp.) cells in boron-free medium: their wall-bound pectin

187 ns using semi-permeabilised mammalian cells (SP cells), in which the ER remains essentially intact an

188 population of cells, called side population (SP) cells, in fresh retinal dissociates.

189 high on CD8 SP but lower or negative on CD4 SP cells, including a subset of CD45RA(+)CD31(-) mature

190 We observed that the cardiac SP cells increase in number more than 3-fold within 3 da

191 cDNA microarray analysis of the SP cells indicated higher expression of ATP-binding cass

192 ansplantation of 5,000 lentivirus-transduced SP cells into lethally irradiated mice resulted in long-

193 c stem cells, the so-called side population (SP) cells, into lethally irradiated mice subsequently re

194 The molecular signature of conjunctival SP cells is consistent with a stem cell phenotype.

195 MUC1 on SP cells is hypoglycosylated and heavily sialylated; the

196 s suggest that the developmental capacity of SP cells is not restricted to the hematopoietic lineages

197 demonstrate that i.v. delivery of genes via SP cells is possible and that these SP cells are capable

198 heterogeneity in the developmental origin of SP cells is reflected in their functional heterogeneity;

199 ich contains about 36% of "Side Population" (SP) cells, is highly enriched for LTR-HSCs.

200 Additionally, our studies revealed that SP cells isolated from dystrophic or cardiotoxin-injured

201 ese cells express surface markers similar to SP cells isolated from skeletal muscle, but differ from

202 Transcriptome analysis of the SP cells isolated from the injured adult murine heart re

203 SP cells isolated from UCB that had been depleted of lin

204 Side-population (SP) cells isolated from limbal and conjunctival epitheli

205 9 yolk sac or para-aortic splanchnopleura (P-Sp) cells, known to give rise to embryonic HSC, similarl

206 n of a relatively small number of transduced SP cells led to prolonged transgene mRNA expression and

207 we demonstrate that the chronically infected SP cell line harbors a relatively low number of provirus

208 Shortly after commitment to CD4 SP cell lineage, monovalent repressive characteristics a

209 the epiphysis of long bone carried the donor SP cell marker.

210 ir low abundance and the absence of specific SP cell markers.

211 the selected genes and signaling pathways of SP cells may be a potential target for effective melanom

212 Compared with non-SP cells, MCF7 SP cells had higher colony-formation abil

213 d a requisite two-step process governing CD4 SP cell medullary localization: the chemokine receptor C

214 We found that the side population (SP) cells (muscle stem cells) injected in isolation surv

215 e, this myogenic activity is associated with SP cells negative for immune (CD45) and vascular (CD31)

216 ansporter inhibition, identifying SP and non-SP cell (NSP) subpopulations by differential staining in

217 ctor resulted in an almost 2 log increase in SP cell numbers over 12 days in culture, whereas there w

218 Raldh2(-/-) hearts display increased SP cell numbers, with selective increases in expression

219 In this system, positive selection of CD4 SP cells occurred only after the addition of exogenous p

220 intrathymic development of long-lived CD4(+) SP cells occurs before the CD4(hi) SP stage of developme

221 ature populations of CD3hiCD4+ and CD3hiCD8+ SP cells of > 99% purity (< 0.3% CD4-containing cells in

222 n (RT-PCR) further revealed up-regulation in SP cells of ABCG2, Sca-1, Wnt-1, and TGF-beta2.

223 ymic SDF-1 inhibited emigration of wild-type SP cells out of the thymus by nullifying the chemokine g

224 Human-derived H1650 SP cells over-express annexin A2 (AnxA2) and SOX2, and a

225 Melanoma SP cells overexpressed ATP-binding-cassette (ABC) transp

226 ata suggest that Akt signaling modulates the SP cell phenotype by regulating the expression of Bcrp1.

227 e confirm that Abcg2 is a determinant of the SP cell phenotype.

228 the adult heart contains an Abcg2 expressing SP cell population and these progenitor cells are capabl

229 s with myogenic cells, some cells within the SP cell population can give rise to early Pax7-positive

230 We propose that the cardiac SP cell population functions as a progenitor cell popula

231 a cytoprotective program for this progenitor SP cell population that resides in the adult heart.

232 eport, we confirm the existence of a cardiac SP cell population, immunophenotypically distinct from b

233 The vast majority of SP cells possessed a primitive cell phenotype (c-kit+, S

234 readily detectable levels, and one-third of SP cells possessed the primitive c-kit+, SCA-1+, lineage

235 his human cancer decreased the proportion of SP cells present and suppressed tumor self-renewal, as i

236 l chemotherapies increased the proportion of SP cells present in tumor xenografts and did not affect

237 standing the molecular regulators of cardiac SP cell proliferation and differentiation, as well as li

238 damage affects the lineage choices of muscle SP cells, promoting their differentiation along fibro-ad

239 Instead, these SP cells rapidly expand giving rise to fibroblast and ad

240 Although it is known that SL and SP cells receive differential inputs from the olfactory

241 y irradiated mice, single gene-marked murine SP cells reconstituted depleted osteoprogenitor pools, s

242 therefore, conclude that CD31-/Sca1+ cardiac SP cells represent a distinct cardiac progenitor cell po

243 Herein we investigated whether SP cells reside within embryonic tissues and exhibit hem

244 6 were either over- or underexpressed in the SP cells, respectively.

245 Moreover, SP cells revealed higher tumorigenicity compared with no

246 pumps, MDR1, MRP1, and ABCG2, in bone marrow SP cells reveals that ABCG2 is the predominant form in t

247 Clonal progeny of single Sca1(+) SP cells show cardiomyocyte, endothelial and smooth musc

248 Most importantly, SP cells showed a markedly higher repopulation and tumor

249 Compared to mixed population cells, H1650 SP cells showed exponential resistance to docetaxel (15-

250 In contrast to non-SP cells, SP cells formed branching structures in matrig

251 A-1+ cells (KTLS), we determined whether the SP cell subset represents a further enrichment in long-t

252 ted with cytokines and lentivirus-transduced SP cells successfully repopulated lethally irradiated C5

253 (PTEN) was confirmed to be critical for MCF7 SP cell survival and proliferation by pathway specific i

254 gnificantly higher drug efflux from leukemic SP cells than from non-SP cells.

255 t retrovirus vectors, effectively transduced SP cells that were not prestimulated with cytokines and

256 population of cells, called side population (SP) cells, that can be isolated with the fluorescence-ac

257 Moreover, we demonstrate that among cardiac SP cells, the greatest potential for cardiomyogenic diff

258 itiating subset were more resistant than non-SP cells to chemotherapeutics that are effluxed by MDR1.

259 The resistance of H1650 SP cells to chemotherapy compared to H1650 MP cells was

260 The inability of BM-SP cells to express this protein severely limits their u

261 ite reports confirming the ability of muscle SP cells to give rise to differentiated progeny in vitro

262 escence-activated cell sorting-sorted cancer SP cells to non-SP cancer cells and normal neural SP cel

263 ntial of SP cells, as well as the ability of SP cells to regenerate original population.

264 understand the potential of skeletal muscle SP cells to serve as precursors for muscle, SP cells fro

265 Notch receptor signaling directed P-Sp cells to T lymphocytes but did not confer lymphopoiet

266 ine receptor CCR7 mediated chemotaxis of CD4 SP cells towards medulla, whereas a distinct pertussis-t

267 Three of 28 SP cell transplants generated overt AML-like disease in

268 re, we examined axonal projections of SL and SP cells using a combination of mouse genetics and retro

269 thymi, yet expansion of both DP and immature SP cells was apparent.

270 ng efficiencies; CLG carrier uptake by H1650 SP cells was demonstrated by fluorescence microscopy, an

271 These SP cells were 6-fold enriched for ERalpha-positive cells

272 Non-SP cells were also derived from marrow stem cells and co

273 However, melanoma SP cells were also resistant to temozolomide, which is n

274 SP cells were also rich in genes associated with stem ce

275 In contrast, the CD34(-)Lin(-) SP cells were CD38(-)HLA-DR(-)Thy-1(-)CD71(-) and failed

276 escriptions of human HSCs, the CD34(+)Lin(-) SP cells were CD38(dim)HLA-DR(dim)Thy-1(dim)CD45RA(-)CD7

277 e, Sca-1-positive SP cells, and the residual SP cells were depleted of repopulating cells in a transp

278 Low but persistent numbers of leukemic SP cells were detected by molecular and immunological as

279 Muscle SP cells were found to be derived from, and replenished

280 Muscle SP and non-SP cells were isolated from Rosa26 mice and directly inj

281 Negatively selected mature SP cells were less abundant in CCX-CKR(-/-) thymi, yet e

282 Even with minimized HSC competition, SP cells were only able to reconstitute up to 35% of rec

283 SP cells were purified from Rosa26 transgenic mice, whic

284 vivo xenograft model, and demonstrated that SP cells were resistant to paclitaxel, a substrate of AB

285 Murine bone marrow SP cells were transduced with HLA-A2.1-expressing VSV-G-

286 Transduced SP cells were transplanted via the tail vein and were sh

287 Different MCAM+ populations, including SP cells, were expanded and assayed for fusion potential

288 cells did not overlap with side-population (SP) cells, were enriched in sphere culture and during ch

289 ture comprising side population (SP) and non-SP cells, where transitions between clonogenic states ar

290 1(-/-) showed a decrease in side population (SP) cells, whereas fatty acid amide hydrolase (FAAH)(-/-

291 en specific CD4+CD25+ Tregs from thymic CD25-SP cells which also required CD28 to maintain their surv

292 or 1a-positive MP cells but has no effect on SP cells, which are BMPR1a-negative.

293 ly purified hematopoietic stem cells and non-SP cells, which do not reconstitute blood.

294 Subsequently, testis side population (SP) cells, which are defined by a Hoechst dye efflux ass

295 It also contains muscle side population (SP) cells, which express Abcg2 and may participate in mu

296 tain an increased number of side population (SP) cells, which show stem cell characteristics, and hav

297 ons that favor the expansion of human muscle SP cells while retaining their myogenic potential have y

298 l fraction of the so-called side population (SP) cells with stem-like tumor-initiating potential.

299 Side-population (SP) cells within cancers and cell lines are rare cell po

300 Microarray analysis of pig limbal SP cells yielded a molecular signature underscoring a ph