ライフサイエンスコーパス: SSB protein

1 ite is much more extensive in the absence of SSB protein.

2 5'-strand is also elevated in the absence of SSB protein.

3 raction of RecR with RecO in the presence of Ssb protein.

4 ' helicase, RecJ, a 5'-->3' exonuclease, and SSB protein.

5 ffects the ssDNA binding mode preferences of SSB protein.

6 change is greatly facilitated by the E. coli SSB protein.

7 occurs after formation of a 1:1 complex with SSB protein.

8 tures may be shared among different types of SSB proteins.

9 omplexes consisting of RecF, RecO, RecR, and Ssb proteins.

10 eractions of RecF protein with RecR and with Ssb proteins.

11 DNA strand exchange in the presence of both SSB proteins.

12 ecies-specific or even specific to bacterial SSB proteins.

13 ntained within the N-terminal domains of the SSB proteins.

14 is unique to Deinococcus and Thermus species SSB proteins.

15 nesium ion, and single-stranded DNA-binding (SSB) protein.

16 tical location of single-strand DNA-binding (SSB) protein.

17 the presence of single-stranded DNA binding (SSB) protein.

18 nd the T7 Gp2.5 single-stranded DNA-binding (SSB) protein.

19 ochondrial single-stranded (ss) DNA-binding (SSB) proteins.

20 , RecQ, RecJ, and single-strand DNA binding (SSB) proteins.

21 vity of their cognate single strand binding (Ssb) proteins.

22 d and protected by binding of ssDNA-binding (SSB) proteins.

23 pitation, size-exclusion chromatography, and Ssb protein affinity chromatography in the absence of an

24 nhibited by the ssDNA products of unwinding; SSB protein alleviates this inhibition.

25 ases to chemo-mechanically push heterologous SSB proteins along ssDNA provides a potential mechanism

26 The RadD and SSB proteins also directly interact in vivo in a yeast t

27 Our results showed that all four SSB proteins also interacted with the MET.

28 Mutations modifying ssDNA-binding (SSB) protein also negate this toxic effect, suggesting t

29 modulating the binding mode of a multimeric SSB protein and consequently, in generating the appropri

30 binding protein encoded by Escherichia coli (SSB protein) and phage T4 (gene 32 protein) also have ac

31 of these two highly conserved homotetrameric SSB proteins, and these differences might be tailored to

32 RecBCD enzyme, single-stranded DNA-binding (SSB) protein, and LexA repressor respond to dsDNA breaks

33 eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial p

34 Mutational studies demonstrated that the Ssb proteins are also required for phage replication, bo

35 roteins, and demonstrate that both SSAPs and Ssb proteins are essential for the life cycle of tempera

36 In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which intera

37 Single-stranded DNA (ssDNA)-binding (SSB) proteins are uniformly required to bind and protect

38 ence of E. coli single-stranded DNA binding (SSB) protein, arguing that LexA repressor affects the co

39 which is not as sensitive to the presence of SSB protein as wild-type RecA protein.

40 In contrast to homotetrameric SSB proteins, asymmetry exists between the two OB folds

41 nsfer of the homotetrameric Escherichia coli SSB protein between ssDNA molecules was studied using st

42 SSB proteins bind to and control the accessibility of si

43 Single-stranded (ss)DNA binding (SSB) proteins bind with high affinity to ssDNA generated

44 ged-helix domain in helicase activity beyond SSB protein binding.

45 richia coli single-stranded (ss)DNA binding (SSB) protein binds ssDNA in multiple binding modes and r

46 This binding occurs in solution and to SSB protein bound to single stranded DNA (ssDNA).

47 h degree of sequence homology with bacterial SSB proteins but differs in the composition of its C-ter

48 of the in vivo concentrations of the SSA and SSB proteins by deletion or overexpression affects HSF a

49 n on ssDNA introduces a new model for how an SSB protein can be redistributed, while remaining tightl

50 vations can be explained by a model in which SSB protein can undergo a temperature- and salt-dependen

51 RecQ helicase, in conjunction with RecA and SSB proteins, can initiate recombination events in vitro

52 he nucleation of RecA protein filaments onto SSB protein-coated single-stranded DNA (ssDNA).

53 fied RecA, RecF, RecO, RecR, RecQ, RecJ, and SSB proteins, components of the RecF system.

54 ase activity is reduced in proportion to the SSB protein concentration; in its absence, ATPase activi

55 strand exchange reaction (especially at high SSB protein concentrations or when SSB protein is added

56 ides, indicating an inhibitory effect of the SSB protein core.

57 umoniae has two single-stranded DNA-binding (SSB) proteins, designated SsbA and SsbB.

58 ecOR protein to ssDNA, which is inhibited by SSB protein despite the documented interaction between R

59 rnary structure analogous to that of E. coli SSB protein,despite possessing DNA-binding domains more

60 nown to stimulate RecO protein to facilitate SSB protein displacement by RecA protein, inhibits annea

61 An enhancement in SSB protein displacement has been shown previously for R

62 s been established for the RecA(Ec) protein, SSB protein does not stimulate the RecA(Sp) protein-prom

63 We show here that single-strand binding (SSB) proteins enhance the unwinding processivity of both

64 d that overexpression of SSB-1 but not other SSB proteins enhanced the HGF-induced serum response ele

65 similarly, this improved ability to displace SSB protein for RecA P67W protein correlates with an inc

66 cts the competition between RecA protein and SSB protein for ssDNA binding sites.

67 sor affects the competition between RecA and SSB proteins for limited ssDNA binding sites.

68 is similar in size to the well characterized SSB protein from Escherichia coli (SsbEc).

69 at were obtained with the well characterized SSB protein from Escherichia coli, SsbEc.

70 ve than wild-type RecA protein in displacing SSB protein from ssDNA.

71 or permits more facile displacement of bound SSB protein from ssDNA.

72 have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataric

73 Here, we show that the structurally similar SSB protein from the malarial parasite Plasmodium falcip

74 s represent the first analysis of paralogous SSB proteins from any bacterial species and provide a fo

75 However, SSB proteins from the Deinococcus-Thermus genera are exc

76 the use of the single-stranded DNA binding (SSB) protein from Escherichia coli as a strong FP signal

77 dislodging of both single-stranded binding (SSB) proteins from ssDNA.

78 trand DNA junctions in vitro, D. radiodurans SSB protein has a limited capacity to displace the short

79 The Escherichia coli SSB protein has a similar but somewhat less robust capac

80 The Deinococcus radiodurans SSB protein has an occluded site size of 50 +/- 2 nucleo

81 These common features for SSB proteins having multiple DNA binding domains enable

82 RecA(Sp) protein from the ssDNA substrate by SSB protein, however, appears to limit the efficiency of

83 al role for either cellular or virus-encoded SSB protein in improving the processivity of the NS3 in

84 Quantitative estimates of D. radiodurans SSB protein in the D. radiodurans cell indicate approxim

85 nce, they offer new insight into the role of SSB protein in the initiation phase of recombination.

86 (RecF-RecO-RecR); (iii) RecF interacts with Ssb protein in the presence of RecO.

87 ly to be relevant to the action of bacterial SSB proteins in double-strand break repair, acting at th

88 e present study, we explored the function of SSB proteins in the regulation of the hepatocyte growth

89 nhibition of DNA strand exchange activity is SSB protein-independent, suggesting that LexA S119A repr

90 However, unexpectedly, SSB protein inhibits both LexA proteolysis and ATP hydro

91 The essential nature of SSB protein interactions makes inhibitors that block SSB

92 y as reagents for investigating the roles of SSB/protein interactions in diverse DNA replication, rec

93 y at high SSB protein concentrations or when SSB protein is added to the ssDNA before RecA(Sp) protei

94 We demonstrate that the crenarchaeal SSB protein is an abundant protein with a unique structu

95 ed lagging-strand polymerase from beta after SSB protein is depleted.

96 at very high concentrations, whereas E. coli SSB protein is highly inhibitory at relative low concent

97 E. coli single-stranded DNA-binding protein (SSB protein) is used to remove secondary structure from

98 lovirus single-stranded DNA (ssDNA)-binding (SSB) protein, LEF-3.

99 Omitting Escherichia coli SSB protein lowers the rate and extent of dsDNA unwindin

100 promoted reaction, the stimulatory effect of SSB protein may be due entirely to this postsynaptic mec

101 radiodurans SSB and homotetrameric bacterial SSB proteins may confer a selective advantage to D. radi

102 richia coli single-stranded (ss)DNA binding (SSB) protein mediates genome maintenance processes by re

103 ant of differences in the effects of Ssa and Ssb proteins on [PSI(+)].

104 scherichia coli single-stranded DNA-binding (SSB) protein on the ability of gp4 to synthesize primers

105 rotein replaced the COOH terminus of E. coli SSB protein or T4 gene 32 protein cannot support the gro

106 the COOH-terminal region from either E. coli SSB protein or T4 gene 32 protein.

107 Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryo

108 ings and suggests the model that the SSA and SSB proteins perform distinct roles in the regulation of

109 in the presence of glucose, suggesting that Ssb proteins, perhaps through their interaction with Reg

110 Single-stranded (ss) DNA binding (SSB) proteins play central roles in DNA replication, rec

111 Single-stranded DNA binding (SSB) proteins play central roles in genome maintenance i

112 scherichia coli single-stranded DNA-binding (SSB) protein plays a central role in DNA replication, re

113 Here we show that SSB protein reduces the level of DNA degradation by RecB

114 restart showed that the replisome-associated SSB protein remains associated with the blocked fork for

115 RecO, and RecR proteins prior to addition of Ssb protein resulted in the formation of complexes consi

116 Stabilization of ITGA4-mRNA with SSB proteins resulted in ITGA4 protein synthesis in HEK2

117 Incubation of RecF, RecO, RecR, and Ssb proteins resulted in the formation of RecF-RecO-Ssb

118 ccus pneumoniae single-stranded DNA binding (SSB) proteins, SsbA and SsbB, to various dT(n) oligomers

119 ccus jannaschii, the Sulfolobus solfataricus SSB protein (SsoSSB) has a single DNA-binding domain in

120 Wild-type gp2.5 and E. coli SSB protein stimulate primer synthesis and DNA-unwinding

121 Thus, we conclude that SSB protein stimulates RecQ helicase-mediated unwinding

122 All bacterial and eukaryotic SSB proteins studied to date oligomerize to assemble fou

123 t the identification of a novel crenarchaeal SSB protein that is distinctly different from its euryar

124 , the causative agent of malaria, encodes an SSB protein that localizes to the apicoplast and likely

125 ir appeared unaffected by alterations in the SSB protein, the mutational analysis suggests a direct r

126 Furthermore, we show that, in the absence of SSB protein, the RecBCD enzyme is inhibited by the ssDNA

127 is coated with single-stranded DNA binding (SSB) protein, thereby accelerating DNA strand exchange.

128 approach relied on the unique ability of the SSB protein to bind the nucleic acid aptamer in its free

129 rved for the binding of the Escherichia coli SSB protein to single-stranded (ss) oligodeoxyadenylates

130 ic Escherichia coli single-stranded binding (SSB) protein to three single-stranded nucleic acids, pol

131 the release of preferentially bound Cl- from SSB protein upon binding nucleic acid, with the release

132 f weakly bound anions (Br- and Cl-) from the SSB protein upon DNA binding.

133 res VirE1-dependent export of ssDNA-binding (SSB) protein VirE2.

134 photocrosslinking, and when Escherichia coli SSB protein was added to the incubations, it bound the s

135 y members function as homotetramers, dimeric SSB proteins were recently discovered in a distinct bact

136 Escherichia coli RecO and SSB proteins, which are functional homologues of Rad52 a

137 cherichia coli stranded DNA-binding protein (SSB) protein, which occurs through stabilizing of the bi