ライフサイエンスコーパス: SSCP

1 SSCP analysis and direct sequencing were used to screen

2 SSCP analysis and microsatellite typing yielded identifi

3 SSCP analysis could distinguish three of the four single

4 SSCP analysis failed to reveal any alterations leading u

5 SSCP analysis of RAPD-PCR markers offers a rapid and ine

6 SSCP and sequencing analysis confirmed the amplification

7 SSCP detects single base changes by altered mobility of

8 SSCP failed to distinguish these variants reliably and t

9 SSCP screening and direct sequence analysis led to the i

10 SSCP screening of the 57 probands with a clinical diagno

11 SSCP thus represents an efficient strategy for mapping t

12 e 60 chromosomes scanned, 14 showed abnormal SSCP mobility shifts.

13 In addition, SSCP analysis permitted rapid carrier detection in two f

14 After SSCP, mobilities were determined by scanning autoradiogr

15 Although SSCP analysis of 62 WT samples and 10 BWS patients did n

16 in the 27 exons of the coding region but an SSCP band shift was seen for PCR products covering the R

17 NA followed by side-by-side comparison on an SSCP gel allows the classification of reamplified materi

18 Using an SSCP platform, we have analysed patterns of transcriptio

19 mational polymorphism-heteroduplex analysis (SSCP-HA) and by direct sequencing.

20 e-strand conformation polymorphism analysis (SSCP) can be utilized for gene mapping in zebrafish.

21 e-strand conformation polymorphism analysis (SSCP) of exon 5 through 9, and by direct sequencing of S

22 e-strand conformation polymorphism analysis (SSCP) was used to screen for potential mutations in pati

23 e-strand conformation polymorphism analysis (SSCP).

24 ing suggested linkage to chromosome 19p, and SSCP showed an aberrant migrating fragment in exon 6 of

25 for p53 gene alterations by western blot and SSCP/DNA sequence analysis and for mdm-2 overexpression

26 Dosage and SSCP analysis of SMNT in this family indicated that the

27 d exons were used to amplify DNA by PCR, and SSCP was then used to screen for mutations.

28 Clinical samples used in VSH and SSCP analyses were subjected to multiple independent amp

29 d as EKK), with CA-repeat markers as well as SSCP polymorphisms.

30 protocol for sample preparation allowed both SSCP and HA species to be produced in one step in a sing

31 ows that TTC4 is expressed in all cases, but SSCP analysis of the coding region of this gene followin

32 ependent degradation domain of HIF-1alpha by SSCP screening and direct sequencing in 35 sporadic clea

33 been detected at codons 163, 175 and 311 by SSCP analysis.

34 se, mutations were detected in all 39 (33 by SSCP and 6 by DNA sequencing).

35 the gene, but demonstrated abnormalities by SSCP.

36 r germ-line mutations in exon 2 of BRCA1, by SSCP analysis and direct sequencing.

37 ison, 16% of samples were miss-classified by SSCP with 25-31% errors in the identification of the wil

38 No mutations were detected by SSCP analysis in a preliminary analysis of 12 subjects r

39 o mutations of the EDA gene were detected by SSCP analysis in the two families not excluded by haplot

40 Four variant classes were determined by SSCP analysis.

41 Somatic point mutations in p53 were found by SSCP in 2/33 papillary thyroid carcinomas, with one miss

42 9%) had mutations within JAG1, identified by SSCP analysis.

43 ct DNA migration patterns were identified by SSCP.

44 adults, were analyzed for PTEN mutations by SSCP assay and sequencing.

45 We detected FLN1 mutations by SSCP in 83% of PH pedigrees and 19% of sporadic females

46 Irish origin for germline CDH1 mutations, by SSCP analysis of all 16 exons and flanking sequences.

47 on polymorphism electrophoresis (SSCP) or by SSCP alone; variants were sequenced directly.

48 s were judged to carry only wild type p53 by SSCP analysis of all exons and sequence determination of

49 ing of 52 patients with PCG was performed by SSCP and direct sequencing of PCR fragments.

50 equence in 97 males from four populations by SSCP.

51 ion to five mutations detected previously by SSCP screening of cDNA, we detected 13 new band 3 gene m

52 Variant DNA fragments revealed by SSCP analysis were subsequently sequenced.

53 Variant bands revealed by SSCP were studied further by polymerase chain reaction-b

54 Screening by SSCP analysis identified mutations in 124 of the kindred

55 found to be essential for resolution of CAE-SSCP/HA peaks and yielded sensitive mutation detection i

56 en analyzed using these optimized tandem CAE-SSCP/HA protocols and materials and yielded 100% sensiti

57 CE-SSCP, a conformation-dependent separation method, was us

58 olymorphism by capillary electrophoresis (CE-SSCP) and confirmed by sequencing.

59 In the current study, we compared our CE-SSCP results with the potential secondary structures pre

60 single-strand conformation polymorphism (CE-SSCP).

61 single-strand conformation polymorphism (CE-SSCP).

62 Since CE-SSCP eliminates the need for probes to have different le

63 this technique, we observed a characteristic SSCP pattern in 29 out of 30 patient samples in the FMLP

64 In contrast, SSCP analysis detected HPV-16 variants that represented

65 In contrast, SSCP and pyrosequencing of an unrelated single SNP (rs18

66 These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient

67 A newly developed SSCP assay enabled us to detect all DNA mutations and po

68 Different SSCP patterns among LDSIs of a strain were associated wi

69 Two to four different SSCP patterns were identified in each of the variable re

70 In both families, differing SSCP patterns were observed in affected and unaffected i

71 d conformation polymorphism electrophoresis (SSCP) or by SSCP alone; variants were sequenced directly

72 e' pedigree was subjected to an exon-by-exon SSCP analysis of the RB1 gene.

73 nd 18 cell lines was analysed by fluorescent SSCP.

74 ielded lower sensitivity on its own (93% for SSCP and 75% for HA).

75 verage molar mass (Mw) and concentration for SSCP/HA peak separation.

76 Forty SSCP abnormalities in 39 tumors included nine deletion,

77 Four samples had SSCP patterns consistent with sequence alterations, sequ

78 on, when 10 to 21 (median, 16) different HDA+SSCP patterns were detected among 33 cDNA clones examine

79 le-stranded conformational polymorphism (HDA+SSCP) analysis, was very high, even during acute HCV inf

80 The sensitivity of the HDA+SSCP method compared favorably with either HDA or the SS

81 ty (avoidance of false detection) of the HDA+SSCP method were very high.

82 ined method is referred to herein as the HDA+SSCP method).

83 This HDA+SSCP method accurately reflected nucleotide sequence div

84 However, SSCP/sequencing analysis excluded mutations of this gene

85 ne or more termination segments (informative SSCP component).

86 We developed protocols for non-isotopic SSCP analysis of PCR products from genomic DNA samples,

87 Ms-SSCP is a rapid and simple technique for screening multi

88 Based on differential band mobility, Ms-SSCP is able to sensitively detect alterations in methyl

89 In the methylation-sensitive SSCP (Ms-SSCP) technique, sodium bisulfite modification of DNA co

90 OVAM-S (detection of virtually all mutations-SSCP) in effect detects all mutations and is less costly

91 rogesterone receptor was carried out, and no SSCP changes were identified.

92 nds with familial Best disease who showed no SSCP shift.

93 found in five cell lines, and nonpolymorphic SSCP and DNA sequence alterations were found within exon

94 PCR products were analyzed by nonradioactive SSCP.

95 her screening methods used for TSC2, notably SSCP, and since PTT involves much less work it may be th

96 ion, and PCR product size) on the ability of SSCP and ddF to detect mutations.

97 tations by PCR amplification and analysis of SSCP.

98 ing frame and identifies three categories of SSCP patterns: those identical to the patterns of protot

99 results yield insight into the mechanism of SSCP and how the conditions of this measurement, especia

100 d that is a highly sensitive modification of SSCP, has successfully detected all of 240 mutations and

101 pecies complexity, assessed as the number of SSCP bands.

102 The sensitivity of SSCP varied with both gel temperature and the size of th

103 xon 5 through 9, and by direct sequencing of SSCP variants to determine the frequency and types of mu

104 These studies document the utility of SSCP analysis for both mutation detection and carrier de

105 orphisms yet identified using either RFLP or SSCP in the coding region.

106 Our SSCP protocol uses silver staining to detect DNA fractio

107 h factor as a candidate gene by carrying out SSCP analysis of each of its 24 exons using a panel of s

108 zation, provides significant advantages over SSCP in screening DNA sequences for the presence of poin

109 In 2 patients, SSCP patterns for pretransplant PBMC-serum pairs were id

110 vised an efficient method, Incorporation PCR SSCP (IPS), for detection of gene alterations.

111 examined the gene and its product by RT-PCR, SSCP, Northern, Southern, and Western blot analysis in p

112 PCR-SSCP analyses revealed a total of six germline missense

113 PCR-SSCP analysis followed by DNA sequencing identified dele

114 PCR-SSCP is a simple and rapid method for the identification

115 PCR-SSCP of 93 samples from a human-hamster radiation hybrid

116 rand conformation polymorphism analysis (PCR-SSCP).

117 We show here that applying PCR-SSCP to a radiation hybrid panel allowed mapping and spe

118 kip1 gene was screened for mutations by PCR-SSCP analysis and/or direct sequencing, while tumour mRN

119 inactivating mutations were detected by PCR-SSCP.

120 resence of extra HLA-DR was confirmed by PCR-SSCP.

121 ved a difference in migration pattern in PCR-SSCP, indicating the presence of at least one point of s

122 To control for genetic instability, PCR-SSCP analysis of exon 2 of the human progesterone recept

123 We conclude that nonradioactive PCR-SSCP for TCR-gamma gene rearrangement analysis is a usef

124 Although the sensitivity of PCR-SSCP analysis is less than 100%, these findings suggest

125 ngle-strand conformational polymorphism (PCR-SSCP) analysis and DNA sequencing.

126 single strand conformation polymorphism (PCR-SSCP) analysis.

127 ngle-strand conformational polymorphism (PCR-SSCP) in paraffin-embedded tissue.

128 single-strand conformation polymorphism (PCR-SSCP) was used to confirm the extra HLA-DR antigens dete

129 is, like DNA sequencing of PCR products, PCR-SSCP (polymerase chain reaction - single strand conforma

130 The development of a rapid PCR-SSCP test for distinguishing M. bovis from M. tuberculos

131 lastoma) for alterations of MAD1L1 by RT-PCR-SSCP and nucleotide sequencing.

132 strand conformation polymorphism (SSCP) (PCR-SSCP) and sequencing.

133 We also applied the PCR-SSCP approach to assay allelic DNA methylation at specif

134 ciated with PAX2 mutations, we have used PCR-SSCP to identify PAX2 gene mutations in patients.

135 cimens for PTEN/ MMAC1 alterations using PCR-SSCP and direct sequencing.

136 PCR/SSCP analysis of exon 2 in p21WAF1/CIP1 detected band sh

137 PCR/SSCP and Southern analysis of one cell line DNA that exp

138 nded conformation polymorphism analysis (PCR/SSCP).

139 Both Southern blot analysis and PCR/SSCP analysis were performed on 63 samples from 56 patie

140 s in 'hot spot' exons 6, 8, 13 and 16 by PCR/SSCP analysis of genomic DNAs from 54 TCC tumor samples

141 p53 mutations were detected by PCR/SSCP in seven tumors [25%] (one superficial, six invasiv

142 red/green color vision genes by a novel PCR/SSCP-based method and assessed expression by mRNA analys

143 ngle-strand conformational polymorphism (PCR/SSCP) analysis of the T cell receptor gamma gene and rel

144 In combination with RT-PCR/SSCP, these analyses allowed the rapid identification of

145 ained from 1990 onwards and subjected to PCR/SSCP.

146 tage of positive samples was higher with PCR/SSCP than with Southern blot analysis (29 of 63 vs eight

147 ing gels and in nondenaturing polyacrylamide SSCP gels.

148 by single-strand configuration polymorphism (SSCP).

149 ons-Single Strand Conformation Polymorphism (SSCP) (DOVAM-S), a robotically enhanced multiplexed scan

150 CR)-single-strand conformation polymorphism (SSCP) (PCR-SSCP) and sequencing.

151 by single-strand conformation polymorphism (SSCP) analysis and confirmed by nucleotide sequencing.

152 Single-strand conformation polymorphism (SSCP) analysis and direct genomic sequencing were used t

153 by single strand conformation polymorphism (SSCP) analysis and DNA sequencing.

154 Single-stranded conformation polymorphism (SSCP) analysis and sequencing were used to evaluate the

155 f single-stranded conformation polymorphism (SSCP) analysis and sequencing.

156 but single-strand conformation polymorphism (SSCP) analysis detected identical band shifts in the leu

157 ing single-strand conformation polymorphism (SSCP) analysis of cDNA markers to identify single nucleo

158 orm single-strand conformation polymorphism (SSCP) analysis of FLN1 throughout its entire coding regi

159 by single-strand conformation polymorphism (SSCP) analysis of genomic DNA and subsequent nucleotide

160 by single-strand conformation polymorphism (SSCP) analysis of p53 exons 4 to 8.

161 sed single-strand conformation polymorphism (SSCP) analysis of p53 exons 4-8 to screen for possible m

162 of single strand conformation polymorphism (SSCP) analysis of RAPD markers to generate linkage maps

163 h single-stranded conformation polymorphism (SSCP) analysis of RT-PCR products, elucidated the develo

164 and single strand conformation polymorphism (SSCP) analysis of the entire open reading frame (ORF).

165 Single-strand conformation polymorphism (SSCP) analysis of the NBR2 gene failed to identify any m

166 is, single-strand conformation polymorphism (SSCP) analysis, and direct DNA sequencing were performed

167 , single-stranded conformation polymorphism (SSCP) analysis, and DNA sequencing, we examined the cDNA

168 ing single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful

169 g single stranded conformation polymorphism (SSCP) analysis, we screened the PTEN/MMAC1 open reading

170 by single-strand conformation polymorphism (SSCP) analysis.

171 ing single-strand conformation polymorphism (SSCP) analysis.

172 y single-stranded conformation polymorphism (SSCP) analysis.

173 by single-strand conformation polymorphism (SSCP) analysis.

174 ent single-strand conformation polymorphism (SSCP) analysis.

175 ing single strand conformation polymorphism (SSCP) analysis.

176 ld, single-strand conformation polymorphism (SSCP) analysis.

177 ent single strand conformation polymorphism (SSCP) analysis.

178 ith single-strand conformation polymorphism (SSCP) analysis.

179 The single-strand conformation polymorphism (SSCP) and a direct sequencing technique were used to scr

180 of single-strand conformation polymorphism (SSCP) and automated DNA sequencing was used to systemati

181 by single-strand conformation polymorphism (SSCP) and direct sequencing.

182 by single-strand conformation polymorphism (SSCP) and DNA sequence analyses and PCR-based deletion a

183 sed single-strand conformation polymorphism (SSCP) and DNA sequence analysis to identify mutations in

184 ith single-strand conformation polymorphism (SSCP) and heteroduplex mobility assay (HMA).

185 Single-strand conformation polymorphism (SSCP) and nucleotide sequence analyses of genomic DNA re

186 by single-strand conformation polymorphism (SSCP) and nucleotide sequence analyses.

187 PCR single-strand conformation polymorphism (SSCP) and sequence analyses were performed for identific

188 Single strand conformation polymorphism (SSCP) and sequence analysis of TSC1 in bladder tumours a

189 by single-strand conformation polymorphism (SSCP) and sequencing.

190 by single strand conformation polymorphism (SSCP) and used this in mapping studies and to re-investi

191 PCR-single-strand conformation polymorphism (SSCP) assay to differentiate M. bovis from M. tuberculos

192 que single-strand conformation polymorphism (SSCP) can be easily applied to bisulfite-treated DNA to

193 Single strand conformation polymorphism (SSCP) mapping of the TGF-beta1 gene promoter has identif

194 six single-strand conformation polymorphism (SSCP) markers.

195 The single-strand conformation polymorphism (SSCP) method was used to analyze 227 unrelated patients

196 The single-strand conformation polymorphism (SSCP) method was used to search for sequence variants, w

197 PCR-single-strand conformation polymorphism (SSCP) of cDNAs showed no mutation in 43 p73-expressing c

198 CR) single strand conformation polymorphism (SSCP) platform to detect the pattern of expression of 20

199 , a single-strand conformation polymorphism (SSCP) profile could be obtained for each mutation in les

200 The single-strand conformation polymorphism (SSCP) technique and a direct genomic sequencing techniqu

201 The single-strand conformation polymorphism (SSCP) technique and a direct genomic sequencing techniqu

202 The single-strand conformation polymorphism (SSCP) technique and direct sequencing were used to scree

203 Single-strand conformation polymorphism (SSCP) was used to assess mitochondrial variation among s

204 by single strand conformation polymorphism (SSCP) were designed.

205 ng, single-strand conformation polymorphism (SSCP), and random sequencing.

206 by single-strand conformation polymorphism (SSCP), DNA sequencing, restriction analysis, and family

207 and single strand conformation polymorphism (SSCP)-based assay with which allelic sensitivity to nucl

208 by single-strand conformation polymorphism (SSCP).

209 y a single-strand conformation polymorphism (SSCP)/direct DNA sequencing approach.

210 nel single-strand conformation polymorphism (SSCP)/heteroduplex analysis (HA) method, implemented in

211 d single-strand conformational polymorphism (SSCP) analyses were compared for their capacities to det

212 Single strand conformational polymorphism (SSCP) analysis and direct sequencing of TSG101 cDNAs als

213 single-stranded conformational polymorphism (SSCP) analysis and sequencing.

214 R-single strand conformational polymorphism (SSCP) analysis of exons 2-8 in the four groups and direc

215 y single-strand conformational polymorphism (SSCP) analysis on 35 Northern-European Caucasian patient

216 single-stranded conformational polymorphism (SSCP) analysis or major rearrangements by Southern analy

217 d single strand conformational polymorphism (SSCP) analysis to screen two large and nine small LQTS f

218 Single-stranded conformational polymorphism (SSCP) analysis was performed, and PCR products containin

219 d single-strand conformational polymorphism (SSCP) analysis.

220 f single strand conformational polymorphism (SSCP) and dideoxy sequencing to detect the presence of s

221 (single-strand conformational polymorphism (SSCP) and dideoxyfingerprinting (ddF)) were compared to

222 y single strand conformational polymorphism (SSCP) and heteroduplex (HD) analysis of 99% of the codin

223 y single-strand conformational polymorphism (SSCP) and sequence analysis were introduced into express

224 d single-strand conformational polymorphism (SSCP) and sequencing analysis to screen DNA from APL pat

225 single-stranded conformational polymorphism (SSCP) as the dominant TCRBV2-expressing clone among CD8+

226 e single-strand conformational polymorphism (SSCP) assay and direct sequencing in pretransplant-paire

227 single-stranded conformational polymorphism (SSCP) for p53 mutations and by PCR and denaturing gradie

228 single-stranded conformational polymorphism (SSCP) in 22 donor hearts used for transplantation, 10 un

229 Single-strand conformational polymorphism (SSCP) is still a frequently used genotyping method acros

230 single-stranded conformational polymorphism (SSCP) method to determine the number of clonotypes (elec

231 e single-strand conformational polymorphism (SSCP) method.

232 g single strand conformational polymorphism (SSCP) to screen human patched in 37 sporadic BCCs, we de

233 single-stranded conformational polymorphism (SSCP).

234 or single-strand conformation polymorphisms (SSCP) in eight piebald subjects previously reported to b

235 single strand conformational polymorphisms (SSCP).

236 ingle-stranded conformational polymorphisms (SSCPs) in a meiotic mapping panel.

237 RAPD-SSCP analysis revealed segregation of codominant alleles

238 Genotypes were also scored at 57 RAPD-SSCP loci, 5 (TAG)(n) microsatellite loci, and 6 sequenc

239 carcinomas, and five primary tumors revealed SSCP and/or sequence abnormalities in exon 1 (one case)

240 ptosis in 67% of tumor p53 genes using a RNA-SSCP analysis, when only a quarter of them had mutations

241 In the methylation-sensitive SSCP (Ms-SSCP) technique, sodium bisulfite modification

242 Samples demonstrating significant SSCP shifts underwent automated nucleotide sequencing an

243 stability over time, as reflected by stable SSCP band patterns.

244 equence polymorphism, utilizing a stratified SSCP/DNA sequencing protocol.

245 This simple, highly sensitive tandem SSCP/HA mutation detection method should be easily trans

246 ddF is no more technically demanding than SSCP, yet it is more sensitive in detecting point mutati

247 Again, VSH had a higher sensitivity than SSCP analysis in detecting mixed HPV-16 variant infectio

248 Our data suggest that SSCP cannot always detect reliably several closely locat

249 The SSCP analysis of the tumor tissue prior to in vitro cult

250 The SSCP assay used in this comparison targets a 682-bp frag

251 The SSCP pattern for this clone was not apparent among CD4+

252 ) those that need to be reamplified from the SSCP gel before cloning and (iii) those that are too com

253 In 6 patients with resolving hepatitis the SSCP band pattern remained stable, whereas in one patien

254 ible single-base substitutions; and (ii) the SSCP component is enhanced because alterations of mobili

255 ated 100% sensitivity and specificity of the SSCP analysis.

256 On the basis of the SSCP data, phylogenetic analysis of the nucleotide seque

257 optimized to improve the sensitivity of the SSCP method.

258 The speed and simplicity of the SSCP methodology for detection of these mutations make i

259 This contrasted with 100% sensitivity of the SSCP screening methodology.

260 od compared favorably with either HDA or the SSCP method alone, which resulted in intraclonotype dive

261 g region of the beta-spectrin gene using the SSCP technique, in 40 families with HS associated with s

262 this assay to the microchip format where the SSCP analysis is complete in 120 s, representing only a

263 Through SSCP and heteroduplex analysis of genomic DNA, we found

264 donor viruses are then electrophoresed under SSCP conditions.

265 We used SSCP analysis and direct DNA sequencing to screen the en

266 mutations and clinical correlations, we used SSCP analysis to screen DNA from 32 unrelated patients.

267 Thus we used SSCP and stratified DNA sequencing to cover a large numb

268 Using SSCP analysis and sequencing of genomic DNA, we found EB

269 Using SSCP analysis of the SMN exons we screened our SMA patie

270 Using SSCP analysis, we also demonstrated that this clone was

271 n exon by exon screen of the PTEN gene using SSCP failed to identify any mutations in this tumour ser

272 ed individuals for EPOR gene mutations using SSCP analysis and found a C5964G mutation in exon VIII t

273 A single band shift using SSCP was identified in exon 21 which resulted in a misse

274 ed in studies of human cancers which utilize SSCP as the method of mutational screening.

275 of these, 10/10 were detected by ddF, while SSCP detected 6/10 true mutations and falsely identified

276 G4.5 gene for mutations in this family with SSCP and direct sequencing and found a novel glycine-to-

277 tumors were analyzed for p53 mutations with SSCP analysis of the entire open reading frame.

278 Direct sequencing of two BCCs without SSCP variants revealed mutations in those tumours as wel