ライフサイエンスコーパス: Sephadex

1 tion from complex mixtures of RNA using only Sephadex.

2 hromatographies on phosphocellulose and DEAE-Sephadex.

3 series of chromatographies on carboxymethyl-Sephadex and silica gel in chloroform and methanol.

4 imilar to previously observed compression of sephadex beads and ficoll solutions.

5 -14-kDa cutoffs) were juxtaposed between the Sephadex beads and skinned semitendinosus muscle fibers

6 ophils in the inflammation models induced by Sephadex beads and thioglycollate, as well as in an expe

7 aria) skeletal muscle fibers by using single Sephadex beads as osmometers and dialysis membranes as p

8 h favor melanization of parasites as well as Sephadex beads.

9 t glucose moites inside of intensely colored Sephadex beads.

10 A molecules from the glucose residues of the Sephadex beads.

11 ble strains to intrathoracically injected CM-Sephadex beads.

12 Sephadex-binding RNA ligands (aptamers) were obtained th

13 the apparent diffusion coefficient excluding Sephadex, boundary layer thicknesses excluding silica, s

14 o dextran B512, the soluble base material of Sephadex, but not to isomaltose, isomaltotriose and isom

15 proteins could be partially eluted from the Sephadex by low-molecular-weight alpha-1,6 glucan or ful

16 parated by cation-exchange chromatography on Sephadex C25 using sodium (-)-dibenzoyl-l-tartarate as t

17 Intratracheal instillation of 5 mg/kg Sephadex caused a time-dependent eosinophil infiltration

18 Sephadex chromatography separated bound from free labele

19 ty chromatography, and quaternary aminoethyl-Sephadex column chromatography, and the sequence of the

20 an efficient route which bypasses the use of Sephadex column chromatography, is shown.

21 om in vitro translation reaction followed by Sephadex column purification or from heterologous expres

22 diastereomers that separate on chiral-phase Sephadex columns.

23 hed extract (ACs-EEX), anthocyanins-enriched sephadex extract (ACs-EES) and anthocyanidins-enriched e

24 ell that could be removed by passage through Sephadex G-10 and thus may be a macrophage.

25 rotein affinity column and then applied to a Sephadex G-10 column to separate the eluted poly- from t

26 e, Phenyl-Sepharose hydrophobic interaction, Sephadex G-100 and Sephacryl S-200 gel filtration chroma

27 le for these effects was found to elute from Sephadex G-100 gel columns in a fraction with 23,000 mol

28 Sephadex G-100 gel filtration elution profiles of 125I-l

29 ecipitation using ammonium sulphate (0-80%), Sephadex G-100, and Mono Q-Sepharose ion exchange chroma

30 cation - by ammonium sulphate precipitation, Sephadex G-100, and Q Sepharose - was applied to isolate

31 Using Sephadex G-100, the aptamer could be purified from a com

32 rs from both groups showed strong binding to Sephadex G-100.

33 50 M and gel filtration chromatography using Sephadex G-100.

34 DEAE-cellulose, calcium hydroxylapatite, and Sephadex G-150) that yields enzymatically active KatY (2

35 5 to 80 years of age) were fractionated on a Sephadex G-200 column, and the crystallins were tested f

36 Sera were subjected to Sephadex G-200 gel filtration chromatography, and the st

37 matography successively on phenyl-Sepharose, Sephadex G-200, and twice on Mono Q FPLC.

38 obtained by gel filtration chromatography on Sephadex G-200.

39 aphy on DEAE-Sephacel, and gel filtration on Sephadex G-200.

40 Incubation of Sephadex G-25 purified cell extracts of M. thermophila o

41 complexes ( approximately 725 kDa) through a Sephadex G-25 size exclusion column retained their abili

42 was partially purified by gel filtration on Sephadex G-50 and octyl-Sepharose.

43 Sephadex G-50 chromatography of the inactivation mixture

44 Sephadex G-50 chromatography of the inactivation reactio

45 H]-F(2)CDP and reisolation of the protein by Sephadex G-50 chromatography resulted in recovery 0.5 eq

46 or the B3P-G3PDH complex was determined from Sephadex G-50 displacement experiments to be 4:1.

47 Hummel-Dreyer equilibrium chromatography on Sephadex G-50 superfine.

48 renatured, and purified by DEAE-Sephacel and Sephadex G-75 chromatography.

49 of RPH with 5%DH (RPH5) was determined using Sephadex G-75 column.

50 The inactive fraction was removed by Sephadex G-75 gel filtration column chromatography, as i

51 Gel filtration through Sephadex G-75 revealed that wild-type and R91L regA prot

52 uding diethylaminoethyl sepharose (DEAE) and Sephadex G-75 size exclusion columns.

53 EAE-Sephacel, phenyl-Sepharose, S-Sepharose, Sephadex G-75, concanavalin A-agarose, and heparin-Sepha

54 determined to be 40 kDa by gel filtration on Sephadex G-75.

55 ococcus sobrinus were able to bind avidly to Sephadex G-75.

56 est ACE inhibitory activity was separated on Sephadex G10 and two peptides fractions were obtained.

57 fate fractionation, column chromatography on Sephadex G100 and adenosine-agarose, and TSK-250 size-ex

58 small particles made of crosslinked dextran (Sephadex G100), and a glucose- and mannose-specific bind

59 instillation of cross-linked dextran beads (Sephadex G200).

60 The initial extract was loaded into a Sephadex G25 column and fractions showing antioxidant ac

61 m sulfate precipitation, and gel filtration (Sephadex G25).

62 After Sephadex G50 chromatography, the synthase contained appr

63 Sephadex increased T cell numbers (including CD4(+) T ce

64 r prednisolone) in a rodent model of asthma (sephadex-induced eosinophil influx).

65 Sephadex-induced eosinophilia and Th2 cytokine gene and/

66 a pivotal role for T cells in orchestrating Sephadex-induced inflammation in this model.

67 We have studied the time course of Sephadex-induced lung eosinophilia, changes in pulmonary

68 Sephadex-induced lung injury, as measured by (125)I-labe

69 ompared to 1.2 mg/kg for prednisolone in the Sephadex-induced pulmonary eosinophilia model and an ED(

70 Sephadex instillation also induced airway hyperreactivit

71 ct was prepared from pecans and separated by Sephadex LH-20 column chromatography into low- and high-

72 7 column), and fractions (fractionationed by Sephadex LH-20 column) from these two legumes.

73 PE) using size exclusion chromatography with Sephadex LH-20 without the need for any previous SPE of

74 ified by repeated column chromatography over Sephadex LH-20, RP C18 and silica gel.

75 essive HSCCC runs, and final purification on Sephadex LH-20.

76 ocoa beans by ligand-exchange and subsequent Sephadex-LH20 chromatography.

77 ys-C protease, followed by gel filtration on Sephadex LH60 and dual sequencer runs, positioned the 3H

78 cording to peptide size by gel filtration on Sephadex LH60 in formic acid:ethanol.

79 The aptamer was very specific to the Sephadex matrix and did not bind appreciably to other su

80 antiinflammatory activity (evaluated in the Sephadex model of lung edema) with reduced systemic toxi

81 These data suggest that, in the Sephadex model of lung inflammation, eotaxin up-regulati

82 to particles of cellulose (Avicel), but not Sephadex or Sepharose.

83 Intratracheal instillation of Sephadex particles is a convenient model for assessing t

84 The binding of ConA to Sephadex particles results in a significant turbidity in

85 n organ culture were chromatographed on DEAE Sephadex to isolate gangliosides.

86 on) of eosinophils in lung tissue 24 h after Sephadex treatment.

87 ion-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl-3H]

88 Liposomes, silica, and Sephadex were used separately to model the tissue prepar

89 At a medium to high loading degree of Sephadex with Alexa488-Con A (10 mg mL(-1) bead suspensi