ライフサイエンスコーパス: Sepharose

1 finity and ion exchange chromatography (DEAE-Sepharose).

2 recipitates with rhodopsin on Concanavalin A Sepharose.

3 aureum by affinity chromatography on mannose-Sepharose.

4 Ni-NTA-agarose, DEAE-Toyopearl, and heparin-Sepharose.

5 amino acids were immobilized onto activated Sepharose.

6 -5) bind to both m(7)GTP- and m(3)(2,2,7)GTP-Sepharose.

7 ome captured with gamma-phosphate-linked ATP-Sepharose.

8 tography on protein G-Sepharose and factor V-Sepharose.

9 y, and the protein was covalently coupled to Sepharose.

10 parum over hydroxychloroquine- or primaquine-Sepharose.

11 lost their affinities for binding to heparin-Sepharose.

12 n protein binds to hyaluronan immobilized to Sepharose.

13 affinity chromatography by using glutathione-Sepharose.

14 encompassing 2 of 5 motifs bound to heparin-Sepharose.

15 were enriched from HIVIG by using protein A-Sepharose.

16 Both constructs bound to calmodulin-Sepharose.

17 tion over 2',5'-ADP-Sepharose and calmodulin-Sepharose.

18 nd Hop, and to the hydrophobic resin, phenyl-Sepharose.

19 agments of the receptor were retained on PP1-Sepharose.

20 ed PP4R1, and PP4C also bound to microcystin-Sepharose.

21 hanolamine was covalently attached to the CH-Sepharose.

22 ively to the 3i peptide coupled to activated Sepharose.

23 s, i.e. asialofetuin-Sepharose and invertase-Sepharose.

24 0 or 65 and 76 in T4 lysozyme immobilized on Sepharose.

25 ography using phosphoethanolamine-conjugated Sepharose.

26 cubated with antibodies to GFP and protein A-Sepharose.

27 hole cell detergent lysates with glutathione-Sepharose.

28 IgG-Fractogel has clear advantages over IgG-Sepharose.

29 protein on spin columns packed with heparin-sepharose.

30 in binds to type III but not type I collagen-Sepharose 2B affinity columns.

31 ressed proteins were purified by glutathione-Sepharose 4B affinity chromatography and were biochemica

32 oxyl-terminal domain fragment) conjugated to Sepharose 4B and the binding was inhibitable by lactose.

33 as crosslinked to cyanogen bromide-activated Sepharose 4B and used to immunopurify plasma BChE treate

34 g assay results using resveratrol-conjugated Sepharose 4B beads confirmed their direct binding.

35 ults from pull-down assay using 7-methyl-GTP Sepharose 4B beads indicated that PEITC treatment reduce

36 ceptor was linked covalently to activated CH-Sepharose 4B beads.

37 Sepharose 4B chromatography demonstrated preferential in

38 tion and affinity purification with a casein-Sepharose 4B column, copurified with casein kinase activ

39 r arm to a cyanogen bromide (CNBr)-activated-Sepharose 4B matrix.

40 motif coupled to cyanogen bromide-activated Sepharose 4B) binds EcoRI in the absence of Mg2+ and elu

41 to solid supports, including Fe(II).BLM A(5)-Sepharose 4B, Fe(II).BLM A(5)-CPG-C(6), and Fe(II).BLM A

42 urified to near homogeneity using Fn-coupled Sepharose 4B-affinity column chromatography, and amino a

43 e (GST) fusion syntaxin isoforms attached to Sepharose 4B-glutathione beads.

44 akes it a suitable ligand for constructing a Sepharose 4B-L-tyrosine affinity matrix.

45 inding activity, did not bind to galectin-3C-Sepharose 4B.

46 fied fimbriae were coupled to CNBr-activated Sepharose-4B, and the solubilized epithelial cell extrac

47 the l-tyrosine spacer-arm to CNBr-activated-Sepharose-4B.

48 XO was bound to a prototypical GAG, heparin-Sepharose 6B (HS6B-XO), the rate of inactivation for uri

49 d affinity chromatography with a Benzamidine Sepharose 6B column.

50 LPPS was purified using Sepharose 6B gel filtration and dissolved into two major

51 y available thiol-affinity resin (thiopropyl Sepharose 6B) to directly capture free thiol-containing

52 ty chromatography with immobilized mannan on Sepharose 6B, the mosquito MBL was partially purified.

53 a known structure coupled to epoxy-activated Sepharose 6B.

54 d by a resistant plant could not bind to m7G-Sepharose, a result which may point to functional redund

55 s were further tested for binding to poly(U)-Sepharose, a substrate to which wild type hnRNP C1 binds

56 rose hydrophobic interaction, concanavalin A-Sepharose affinity and Superose 12 gel filtration chroma

57 Using hSP-A-coupled Sepharose affinity chromatography and polyacrylamide gel

58 s via Q-Sepharose anion exchange and gelatin-Sepharose affinity chromatography followed by protein id

59 ic interaction, lectin affinity, and heparin Sepharose affinity chromatography followed by SDS-polyac

60 Using IgG-Sepharose affinity chromatography of solubilized zippere

61 Escherichia coli and purified by calmodulin-Sepharose affinity chromatography.

62 bits and purified with corresponding peptide-Sepharose affinity chromatography.

63 und to copurify with cubilin isolated by HDL-Sepharose affinity chromatography.

64 Escherichia coli and purified by glutathione-Sepharose affinity chromatography.

65 ized in insect cells and purified by mannose-Sepharose affinity chromatography.

66 protein, was purified from BBMEC by Ibe10-Ni-Sepharose affinity chromatography.

67 sferase (GST) and purified using glutathione-sepharose affinity chromatography.

68 ivity; and (iii) Ndk failed to bind to a Ugi-Sepharose affinity column that tightly bound E. coli ura

69 oli and purified through a pterin-conjugated Sepharose affinity column.

70 ect isolation of PP1-binding proteins on PP1-Sepharose affinity columns.

71 e substrate was immobilized on a glutathione-Sepharose affinity matrix and used to measure wild-type

72 0 point mutant bound to a p-aminobenzoyl-Arg-Sepharose affinity resin, and this binding was competed

73 By using heparin-Sepharose affinity to discriminate between the monomer a

74 % yield by ion exchange chromatography on SP-Sepharose, affinity chromatography on immobilized asialo

75 s an RNA-binding protein that binds to m7GTP-Sepharose, albeit with lower specificity and affinity fo

76 cell culture host range and bound to heparin-Sepharose, although it did not require cell surface HS a

77 PAK3 and p70 S6 kinase bound to microcystin-Sepharose (an affinity resin for PP2A-PP1).

78 tions, three additional proteins bind to WGA-Sepharose and are revealed by the organelle trap assay.

79 In vitro synthesized FLI bound to actin-Sepharose and binding was reduced by competition with ex

80 -linked peptide was further purified by DEAE-Sepharose and C18 column chromatography and subjected to

81 or after partial purification over 2',5'-ADP-Sepharose and calmodulin-Sepharose.

82 modified intein as affinity tags and the Ni sepharose and chitin as affinity matrices.

83 Using heparin-Sepharose and DNA-specific columns, we partially purifie

84 lded a RegA69-His6 protein that binds nickel-Sepharose and elutes with 0.5 M imidazole.

85 amplification, selecting for affinity to Phe-Sepharose and elution with free l-phenylalanine.

86 uential affinity chromatography on protein G-Sepharose and factor V-Sepharose.

87 Bean' by anion-exchange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75.

88 recipitation and chromatography through DEAE-Sepharose and gel filtration columns.

89 ough two affinity columns, i.e. asialofetuin-Sepharose and invertase-Sepharose.

90 Fas binds to CaM-Sepharose and is co-immunoprecipitated with CaM.

91 sing ammonium sulfate fractionation, heparin-Sepharose and Mono Q chromatography, and BTE-affinity ma

92 ecreted factor, which is absorbed by gelatin-Sepharose and reconstituted with MMP-9.

93 0-fold from PE-treated TFG2 cells by heparin-Sepharose and RNA affinity chromatography.

94 neumoniae by serial chromatography with DEAE-Sepharose and Sephacryl S-200.

95 y affinity chromatography on CTGF coupled to Sepharose and sequence information obtained by mass spec

96 purified by affinity chromatography on tRNA-Sepharose and shown to be phosphorylated on the alpha, b

97 sulfate and chromatographed on HiTrap Phenyl Sepharose and Superdex 200 HR 10/30.

98 Laminin-Sepharose and syntrophin-Sepharose bind a protein comple

99 ically coupled to cyanogen bromide-activated Sepharose and the temperature dependence of transcriptio

100 ted only very weak interactions with m(7)GTP-Sepharose and, rather than binding eIF4G, interacted wit

101 age of GST-GLTP fusion protein (bound to GST-Sepharose) and affinity purification resulted in fully a

102 Using metal affinity (chelating Fe(3+)-Sepharose) and anion exchange (POROS HQ 20) chromatograp

103 ins (TrxNT) were purified by ion exchange (S-Sepharose) and/or Talon metal affinity chromatography.

104 ngle step using a cation-exchanger resin, SP-Sepharose, and a selected buffer pH, depending on the pI

105 es on DEAE-Sepharose, hydroxyapatite, phenyl-Sepharose, and chromatofocusing.

106 compared with bead-based matrices, Protein A Sepharose, and Dynabeads Protein A.

107 ite lysates, immunoprecipitated on protein G-Sepharose, and eluted with Laemmli sample buffer.

108 rified by chromatography on DE-52 and phenyl-Sepharose, and its steady-state kinetic constants were d

109 lowed by Q-Sepharose, blue Sepharose, phenyl-Sepharose, and MonoS column chromatography.

110 the full-length type-I short bound more CaM-Sepharose, and this binding was inhibited to a greater e

111 om the urine of breast cancer patients via Q-Sepharose anion exchange and gelatin-Sepharose affinity

112 23 and decreased affinity for Hop and phenyl-Sepharose, are brought on by ATP binding alone.

113 When conjugated to Sepharose as mock therapeutic immmunoaffinity columns, t

114 EGF121 showed increased retention on heparin-Sepharose at pH 5.5 compared with pH 7.5.

115 activities, but they all eluted from heparin-Sepharose at significantly higher ionic strengths compar

116 Incubation of Sepharose bead-bound GST-TNF-R1(207)(-)(425).4D/E fusion

117 display libraries by panning with hyaluronan-Sepharose beads and assayed for their ability to block f

118 ies were performed using bFGF immobilized on Sepharose beads and soluble 125I-labeled fibrinogen and

119 Experiments using IL-1 immobilized on Sepharose beads and soluble iodine 125 ((125)I)-labeled

120 e 165 amino acid form of VEGF immobilized on Sepharose beads and soluble iodine 125 ((125)I)-labeled

121 Modified immunoseparation with protein A-Sepharose beads bound to the LAP-specific monoclonal ant

122 ly, VP35 bound to poly(rI) . poly(rC)-coated Sepharose beads but not control beads.

123 In this system, Sepharose beads coated with Ran induce the formation of

124 Sepharose beads coated with stimulatory but not nonstimu

125 signaling molecule as anti-CD154-conjugated Sepharose beads costimulated B cell responses induced by

126 +) and knockout (CCR1-/-) mice by embolizing Sepharose beads coupled to the purified protein derivati

127 a was confirmed by precipitation with leptin-Sepharose beads followed by immunobloting with anti-lept

128 ted by pull-down of both isoenzymes with CaM-Sepharose beads from 1321N1 cell lysates.

129 binding site: IQ1 interacts with calmodulin-Sepharose beads in a Ca(2+)-independent manner, whereas

130 ose using a surface of fibrin immobilized on Sepharose beads in a similar system.

131 .co-receptor complexes attached to Protein G-Sepharose beads in the absence of target cells.

132 organisms, RgpA, RgpB, and Kgp conjugated to sepharose beads reduced opsonophagocytosis of P. gingiva

133 Under conditions in which avidin-Sepharose beads specifically adsorbed biotinylated Atp11

134 use the antibodies immobilized on protein A-Sepharose beads to bind and purify the enzyme.

135 By adding streptavidin-Sepharose beads to GL-biot-treated DU145 cell lysates, S

136 obilization of sortase A on a solid support (Sepharose beads).

137 ing homopolymeric regions are immobilized on Sepharose beads, and then extension-signal detection-dep

138 ry Fc-specific antibody, covalently bound to Sepharose beads, is used to capture the primary antibody

139 These antibodies, when coupled to Sepharose beads, retained saxitoxin binding sites from s

140 ine and soybean trypsin inhibitor-conjugated Sepharose beads, which bind fXa and sequester it away fr

141 lutathione S-transferase NHE-RF bound to GSH-Sepharose beads.

142 ER-glycosylated peptides onto Con A-coupled Sepharose beads.

143 8 heavy chain monoclonal antibody coupled to Sepharose beads.

144 he subsequent lung challenge with PPD-coated Sepharose beads.

145 urified and immobilized onto thiol-activated Sepharose beads.

146 li type 1 fimbrial binding to mannose-coated Sepharose beads.

147 Laminin-Sepharose and syntrophin-Sepharose bind a protein complex containing Rac1 from th

148 om trypsin cleavage and increased the phenyl-Sepharose binding of a recombinant DGK alpha fragment th

149 Dose-response curves and heparin-Sepharose binding suggested Ad.hFX has greater affinity

150 Using gel overlay assays and CaM-Sepharose binding, we show that the bacterially expresse

151 h phenyl-Sepharose, indicating that a phenyl-Sepharose-binding region (PSBR) of recS100A1 had been di

152 X-100 extraction of membranes followed by Q-Sepharose, blue Sepharose, phenyl-Sepharose, and MonoS c

153 The first peptide when coupled to Sepharose bound the alpha(4)beta(1) integrin directly wh

154 sults, and affinity chromatography utilizing Sepharose-bound antibodies confirmed recognition of the

155 The B cell stimulatory activity of Sepharose-bound sODN did not result from free sODN relea

156 of caveolin-1 from mammalian cell lysates to Sepharose-bound, NSP4-specific synthetic peptides confir

157 -Dynabeads have improved properties over IgG-Sepharose but are generally expensive.

158 vitro and in vivo by PEN3 pull-down with CaM Sepharose, CaM overlay assay and bimolecular fluorescenc

159 partial purification of tobacco GalAT by SP Sepharose cation exchange chromatography and by detectin

160 hieved by sequential chromatography using SP-Sepharose cation exchange, phenyl-Sepharose hydrophobic

161 pH 7.5), and hydrophobic interaction (phenyl-Sepharose) chromatographies.

162 the N- terminal domains purified by m(7)GTP-Sepharose chromatography and polyacrylamide gel electrop

163 olated from plasma of the proband by heparin-Sepharose chromatography contained amounts of beta antit

164 a Ca(2+)-dependent manner as measured by CaM-Sepharose chromatography or a dansyl-CaM fluorescence as

165 eparated and present in "fraction 42" from Q-Sepharose chromatography specifically phosphorylated GRP

166 nin-1 fragments were fractionated by heparin-Sepharose chromatography.

167 acts of acetone-grown R. rhodochrous by DEAE-Sepharose chromatography.

168 artially purified by hydroxyapatite and blue Sepharose chromatography.

169 ex G-75, concanavalin A-agarose, and heparin-Sepharose chromatography.

170 ven in the presence of salt) followed by EAH-Sepharose chromatography.

171 We report that crosslinked agarose (e.g., Sepharose) chromatography medium that has been chemicall

172 strain was optimally immobilised on Glyoxyl-Sepharose CL 4B using the Rational Design of Immobilised

173 le extracellular arabinoxylans, monitored on Sepharose CL-2B, peaked suddenly and transiently, typica

174 hobic-interaction chromatography with phenyl-Sepharose CL-4B and gel filtration with Sephacryl S-200.

175 After Sepharose CL-4B chromatography, newly synthesized sclera

176 rated by molecular sieve chromatography on a Sepharose CL-4B column.

177 ynthesized proteoglycans were separated on a Sepharose CL-4B molecular sieve column and identified by

178 as three major peaks after chromatography on Sepharose CL-4B.

179 s then separated on a SEC column packed with Sepharose CL-4B.

180 By Sepharose CL-6B chromatography, the phosphatase activity

181 m the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by

182 enrichment and the DNA was extracted using a Sepharose CL-6B spin column.

183 48 and AIV, were of the same size as PCTV on Sepharose CL-6B, and by electron microscopy, excluded ca

184 everal chromatographic steps, including DEAE-Sepharose CL-6B, hydroxyapatite, strong anionic and cati

185 Size was assessed by gel filtration on Sepharose CL2B and charge by ion-exchange chromatography

186 ient centrifugation and size fractionated on Sepharose CL2B.

187 The lipase was bound to a butyl Sepharose column and eluted in a Triton X-100 gradient.

188 onomers (approximately 40 kDa) bound to DEAE-Sepharose column and protein complexes (approximately 30

189 n binding, MIP-1beta-A10C binds to a heparin-Sepharose column as tightly as the wild type protein and

190 Heparin-Sepharose column chromatography and analytical ultracent

191 ton X-114-soluble fraction followed by octyl-Sepharose column chromatography and preparative high per

192 sity gradient ultracentrifugation and lysine-Sepharose column chromatography using solutions containi

193 In addition, native mGluR4 was retained on a Sepharose column covalently grafted with recombinant Mun

194 well as the flowthrough fraction of a green Sepharose column during fractionation of the growth medi

195 A Q-Sepharose column flowthrough fraction of the growth medi

196 Incubation of the Q-Sepharose column flowthrough fraction with antiazurin an

197 ants, but not of the wild type, to a heparin-Sepharose column produced binding comparable to that of

198 -human lumican antibody-conjugated protein A Sepharose column was used to purify soluble lumican prot

199 be isolated from chick embryos on a gelatin-Sepharose column, indicating some involvement in the bio

200 be isolated from chick embryos on a gelatin-Sepharose column, indicating some involvement in the bio

201 ographic steps, including 2 steps of heparin-Sepharose column, is reported.

202 inity chromatography on an anti-SOD antibody-Sepharose column.

203 proteineous properties when applied on Con-A Sepharose column.

204 in liquid chromatography (FPLC) on a beta-CD-Sepharose column.

205 ty purified using an immobilized glutathione-Sepharose column.

206 In this study we used heparin-Sepharose columns to demonstrate that PVC-211 MuLV, but

207 pha co-migrates with GST-EF-3 on glutathione-Sepharose columns.

208 efficiently removed from the media by nickel-Sepharose, consistent with the 6-His tag incorporated in

209 ay revealed that the eluate from C16-serinol-Sepharose contained three serine/threonine-specific prot

210 binding to PP1 by overlay and by glutathione-Sepharose coprecipitation assays.

211 te cancer LNCaP cell extract using protein A-Sepharose coupled with anti-AR antibody indicates that t

212 hromatographies, including diethylaminoethyl sepharose (DEAE) and Sephadex G-75 size exclusion column

213 was performed using Con-A Sepharose, Phenyl Sepharose, DEAE Sephacel, and Superdex 75 FPLC.

214 EG precipitation, affinity chromatography on Sepharose derivatized with acetylated serum albumin, ion

215 fication using three different columns (DEAE-Sepharose, Econo S, and heparin-agarose), LRP was primar

216 columns, the immobilized trisaccharide (DSG-Sepharose) eliminates anti-GQ1b antibodies from positive

217 Using heparin-Sepharose-enriched fractions that hybridized to sarA mRN

218 devoid of negatively charged N-glycans by Q-Sepharose exclusion and failure to bind human serum amyl

219 Some isoforms (e.g. IFE-3) bind to m(7)GTP-Sepharose exclusively, whereas others (e.g. IFE-5) bind

220 a spacer of about half the length of the SP Sepharose Fast Flow, and the other contained no spacer a

221 commercial product, Amersham Biosciences SP Sepharose Fast Flow, containing a six-carbon spacer betw

222 ough a 6xHis-Ni2+-NTA affinity column and SP Sepharose/fast protein liquid chromatography.

223 Affinity purification using factor H Sepharose followed by immunoprecipitation using mAbs to

224 Da doublet from human placenta using poly(U)-Sepharose, followed by preparative sodium dodecyl sulfat

225 Finally, beta-CD-Sepharose FPLC was used to isolate prenylated mCherry-CA

226 lens alpha-crystallin was immobilized on EAH-Sepharose gel and glycated using d-ribose.

227 Binding experiments using heparin-Sepharose gel demonstrated that the lipid-free 10-kDa fr

228 palustris by sequential Q-Sepharose, phenyl-Sepharose, gel filtration, and hydroxyapatite chromatogr

229 ype-III IP(3)Rs did not bind directly to CaM-Sepharose, hetero-oligomers of type-I/III IP(3)Rs retain

230 y using SP-Sepharose cation exchange, phenyl-Sepharose hydrophobic interaction, concanavalin A-Sephar

231 to near electrophoretic homogeneity using Q-Sepharose, hydroxyapatite and Mono Q chromatography.

232 o sequential column chromatographies on DEAE-Sepharose, hydroxyapatite, phenyl-Sepharose, and chromat

233 in B to proanthocyanidins indicated that the Sepharose immobilized PACs have a binding affinity for L

234 ing to fibrin using (125)I-labeled VEGF- and Sepharose-immobilized fibrin monomer.

235 Binding of (125)I-IL-1beta to Sepharose-immobilized fibrinogen also demonstrated a sin

236 luble 125I-labeled fibrinogen and also using Sepharose-immobilized fibrinogen and soluble 125I-bFGF.

237 uman T cell nucleolar extracts that bound to Sepharose-immobilized H1 in vitro.

238 en purified by affinity chromatography using Sepharose-immobilized kiwi PME inhibitor protein to obta

239 ied RK(13) cell CRT (rCRT) selectively bound sepharose-immobilized L,D-MDP and PG, but not L,L-MDP or

240 was capable of calcium-dependent binding of Sepharose-immobilized L,D-MDP or peptidoglycan.

241 14 of RK(13) cells did not specifically bind Sepharose-immobilized L,D-MDP.

242 By using a Sepharose-immobilized lectin, concanavalin A, we isolate

243 calized with activated Rac1 and with laminin-Sepharose in solid-phase binding assays.

244 , horseradish peroxidase-labeled CaM and CaM-Sepharose in the presence of Ca2+ whereas EGTA, a Ca2+ c

245 n fact, observe increased binding to m(7)GTP-Sepharose in the presence of eIF4G(557-646), but only wi

246 e gradient centrifugation and eluted from 4B Sepharose in the region of 170,000 mol wt proteins.

247 at the estrogen receptor binds to calmodulin-Sepharose in vitro.

248 serum samples using staphylococcal protein A-Sepharose indicated that antibody-complexed tracer was r

249 Affinity chromatography on m(7)GTP-Sepharose indicates that the larger forms of 4E-BP1 bind

250 ta85-93 protein did not interact with phenyl-Sepharose, indicating that a phenyl-Sepharose-binding re

251 I and observed that it segregates over DEAE-Sepharose into two subcomplexes (termed I-L and I-U).

252 lls, Sephacryl-S 300 gel filtration and DEAE-Sepharose ion exchange chromatography demonstrated that

253 sulphate (0-80%), Sephadex G-100, and Mono Q-Sepharose ion exchange chromatography.

254 Immunoglobulin G (IgG)-Sepharose is often used for purification of protein A- a

255 Further affinity purification over ATP-Sepharose isolated PP-1bp80 in a quaternary complex with

256 od based on interactions of GroEL with octyl-Sepharose, it was demonstrated that Zn2+ binding strengt

257 kinase by affinity chromatography using the Sepharose-linked protein kinase inhibitor, bisindolylmal

258 a trypanosome lysate was adsorbed to an ATP-sepharose matrix and washed with a high salt solution fo

259 omatography with DE52, Affi-Gel Blue, phenyl-Sepharose, MonoQ, and Superdex 200.

260 ed by the copurification on immunoglobulin G-Sepharose of Nip7p with protein A-tagged Rrp43p and Nop8

261 strength, CLDCs bind to heparin-derivatized Sepharose particles, with the ratio of cationic lipid to

262 ration (cutoff, 30 kDa), anionic exchange (Q-Sepharose, pH 7.5), and hydrophobic interaction (phenyl-

263 rther purification was performed using Con-A Sepharose, Phenyl Sepharose, DEAE Sephacel, and Superdex

264 n of membranes followed by Q-Sepharose, blue Sepharose, phenyl-Sepharose, and MonoS column chromatogr

265 m Rhodopseudomonas palustris by sequential Q-Sepharose, phenyl-Sepharose, gel filtration, and hydroxy

266 on-specific clones was employed, using SHP-2 Sepharose, prior to in vitro phosphorylation and selecti

267 using Escherichia coli-expressed and phenyl-Sepharose-purified CaM mutants revealed that the S81A mu

268 hemically synthesized peptides conjugated to Sepharose, residues 1 to 14 and 26 to 37 of mouse p19(AR

269 conjugate was achieved using betaCD-modified sepharose resin.

270 rce 15Q and 0.9561, 0.9876, and 0.9760 for Q Sepharose resins in the presence of three different salt

271 d for NOS proteins by precipitation with ADP-sepharose, revealed expression of endothelial NOS (eNOS

272 Analysis of proteins bound to m(7)GTP-Sepharose reveals that both CGP and eIF4G(1357-1600) dec

273 sulfate fractionation, DEAE-Sephacel, phenyl-Sepharose, S-Sepharose, Sephadex G-75, concanavalin A-ag

274 ciably to other supporting matrices, such as Sepharose, Sephacryl, cellulose or pustulan.

275 ionation, DEAE-Sephacel, phenyl-Sepharose, S-Sepharose, Sephadex G-75, concanavalin A-agarose, and he

276 P4 complex was purified by sequential phenyl-Sepharose, Source 15Q, DEAE2, and Superdex 200 gel filtr

277 was maintained by chromatography on beta-CD-Sepharose, such that the resulting protein could be sele

278 binding to a cap separated by a linker from Sepharose, suggesting differences in the arrangement of

279 edly, but DS displaced thrombin from heparin-Sepharose, suggesting that DS and heparin share a restri

280 ndent Cdk2/Cdk6 phosphatase activity in DEAE-Sepharose, Superdex-200, and Mono Q chromatographies.

281 ces cerevisiae Stu2, covalently coupled to a Sepharose support.

282 ion profiles of APC derivatives from Heparin-Sepharose supported this conclusion.

283 TE bound better to heparin-Sepharose than 633, but this difference was not pH depen

284 After affinity purification on glutathione-Sepharose, the fusion proteins were labeled with [alpha-

285 reated with N-ethylmaleimide (NEM) or phenyl-Sepharose to inactivate or deplete Crm1, respectively, i

286 mediating cell density sensing, we used CMF-Sepharose to isolate membrane proteins that bind CMF.

287 affinity by elution of RNAs from tryptophan-Sepharose using free L-tryptophan evokes one sequence pr

288 Lp(a) binds lysine-Sepharose via a lysine binding site (LBS) located in KIV

289 dition, the binding of the short form to CaM-Sepharose was inhibited by phosphorylation with protein

290 Heparin-Sepharose was used to characterize heparin-GST-LEDGF bin

291 ulphate precipitation, Sephadex G-100, and Q Sepharose - was applied to isolate trypsin, and resulted

292 ressed in 293 cells was coupled to protein A-Sepharose, we have isolated from rat brain at least two

293 of GH3 nuclear proteins that bind to heparin-Sepharose, we have shown that Ets-1 and GABP, which are

294 chromatography with MreC covalently bound to Sepharose, we isolated several PBPs from cell extracts t

295 ed calcium-dependent interaction with phenyl-Sepharose when compared with recS100A1, demonstrating th

296 osomucoid (ASOR)-, anti-H1-, or anti-H2-COOH-Sepharose, whereas only H1 and H2b were immunoprecipitat

297 cytes and eggs via chromatography on heparin-Sepharose, whereas we isolated chromatinized histones vi

298 Moreover, these mutants bound to heparin-Sepharose with lower affinities.

299 2 that was selectively captured by protein G Sepharose, with the tag being removed subsequently using

300 heese whey and further purified through DEAE-Sepharose XK26.