ライフサイエンスコーパス: T-DNA

1 T-DNA and ALC loci were not linked, as indicated by rand

2 T-DNA and transposon-based mutagenesis has been widely e

3 T-DNA and virulence proteins presumably form T-complexes

4 T-DNA inactivation of both Tric proteins further resulte

5 T-DNA insertion lines for ZML2 and its homolog ZML1 demo

6 T-DNA insertion mutants (atg mutants) of these genes dis

7 T-DNA insertion mutants are a tool used widely in Arabid

8 T-DNA insertion mutants have been widely used to define

9 T-DNA insertions in At3g50740 cause a sugar-insensitive

10 T-DNA insertions in each of these genes were identified,

11 T-DNA insertions in the genes represented by some cDNAs

12 T-DNA insertions in UKL1 and UKL2 reduced transcript exp

13 T-DNA insertions were identified in four of the five Ara

14 T-DNA inserts are stable; no transgene rearrangements we

15 T-DNA knock-out mutants of At3g57630 showed a truncated

16 T-DNA knockout of AtcpFHy/PyrP1 did not affect the flavi

17 lanking sequence tags (FST) for over 325,000 T-DNA insertion lines.

18 336,000 T-DNA transformed lines were screened for mutants with d

19 id occur in plants homozygous for the dcl3-1 T-DNA insertion and was unaffected by loss of function o

20 KK3, all located within 20 kb of the mekk1-1 T-DNA insertion.

21 o add to these resources we sequenced 21 165 T-DNA lines, 15 569 of which were produced in this study

22 HC-Pro transgenic Arabidopsis and the arf8-6 T-DNA insertion mutant showed little effect on the P1/HC

23 A T-DNA insertion in Arabidopsis thaliana pdTPI resulted i

24 A T-DNA insertion into the Arabidopsis gene (At5g14090) mo

25 A T-DNA insertional allele of the single NEDD1 gene was is

26 A T-DNA insertional atos9-t mutation blocks the degradatio

27 A T-DNA knock-out line did not segregate homozygous mutant

28 A T-DNA mutagenesis screen performed on haploid spores ide

29 A T-DNA mutant eif4a1 line, with reduced eIF4A protein lev

30 A T-DNA-tagged null mutant mtppt-1 allele shows an embryo-

31 localization and the phenotypes of rh3-4, a T-DNA insertion mutant allele of RH3.

32 We next analyzed a T-DNA insertion in the MEKK2 promoter region and found t

33 erance of an ERF74 overexpression line and a T-DNA insertion mutant using flow cytometry, transactiva

34 distachyon, we identified a line carrying a T-DNA insertion in one of the two eukaryotic initiation

35 Homozygous plants carrying a T-DNA insertion mutation in AtSPP, spp-2, could not be r

36 rk, Arabidopsis thaliana plants containing a T-DNA disruption of the bile acid sodium symporter BASS6

37 d dark-brown phenotype was identified from a T-DNA-tagged rice mutant library.

38 dopsis thaliana) pex5-10 mutant, which has a T-DNA insertion in exon 5 of the PEX5 gene.

39 reactive oxygen species were monitored in a T-DNA insertion mutant of AHK5.

40 Seedling growth was severely reduced in a T-DNA insertion mutant of ICE1, ice1-2, when grown on 1/

41 Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thal

42 RTP1 was identified by screening a T-DNA insertion mutant population and encoded an endopla

43 AAP2 T-DNA insertion lines showed changes in source-sink tran

44 ovement in response to root flooding in aco5 T-DNA insertion mutants.

45 KEY MESSAGE: This study addresses T-DNA insert stability and transgene expression consiste

46 This study addresses T-DNA insert stability and transgene expression consiste

47 s the major model proposed for Agrobacterium T-DNA integration into the plant genome.

48 proteins required for NHEJ, in Agrobacterium T-DNA integration.

49 combinase was delivered on the Agrobacterium T-DNA injected at the axillary bud site, resulting in th

50 is is typically achieved using Agrobacterium T-DNA, biolistics or by stably integrating nuclease-enco

51 Allelic T-DNA lines differed for most traits at AT1G11060 but no

52 a putative function of AtVDACs, we analyzed T-DNA insertion lines in each of the corresponding genes

53 Missense (shot1-1) and T-DNA insertion (shot1-2) mutants suppress the hot1-4 he

54 pressing the wild type version of bZIP16 and T-DNA insertion mutants for bZIP68 and GBF1 demonstrated

55 ng term transgene expression consistency and T-DNA insert stability can be achieved in sugarcane, sug

56 ng term transgene expression consistency and T-DNA insert stability can be achieved in sugarcane, sug

57 The discrimination between glu-5-hmC-DNA and T-DNA by DB-JBP1 is about 2-fold less, but enough for DB

58 eutrons (used for the induction of rfc4) and T-DNA (used for the induction of ife, which is not linke

59 From a collection of transposon and T-DNA insertion lines at the RIKEN chloroplast function

60 were characterized, along with wild-type and T-DNA knock-down plants.

61 eport the characterization of an Arabidopsis T-DNA insertion mutant of the MED14 gene.

62 le of functionally complementing Arabidopsis T-DNA insertion mutants in this gene.

63 We obtained Arabidopsis T-DNA knockout lines for the two genes and analyzed thei

64 Two Arabidopsis T-DNA insertion mutant lines with insertions in the prom

65 Two Arabidopsis T-DNA insertion mutants were identified as null mutants

66 We used Arabidopsis T-DNA insertion lines to generate a double mutant in whi

67 Using Arabidopsis T-DNA insertion mutants, we found that their Arabidopsis

68 o transport measurements, analyses of atmrp1 T-DNA insertion mutants of Arabidopsis ecotypes Wassilew

69 atmyb2 T-DNA insertion lines have enhanced expression of cytoki

70 sion of AtSLD1 in Arabidopsis plants, AtSLD1 T-DNA mutants showed large reductions in Delta8 unsatura

71 Although all six single AtUXS T-DNA mutants and the uxs1 usx2 uxs4 triple mutant show

72 Through examining all available T-DNA insertional mutants of Arabidopsis MCTPs, we furth

73 tumefaciens requires the import of bacterial T-DNA and virulence proteins into the plant cell that ev

74 nstrate that catalytically active Osa blocks T-DNA transfer into plants.

75 Whereas depletion of B14.7 by T-DNA insertion is lethal, Tim23-2 can be depleted witho

76 Disruption of AtPAT14 by T-DNA insertion resulted in an accelerated senescence ph

77 of the DUF579 family have been disrupted by T-DNA insertions contain less xylose in the secondary ce

78 nent), which on simultaneous inactivation by T-DNA insertion lines displayed a severely delayed and c

79 lt plant while knocking down miR408 level by T-DNA insertions or the artificial miRNA technique cause

80 er to further investigate the route taken by T-DNA molecules on their way to integration.

81 Plants carrying T-DNA insertions in three AtGATL genes (atgatl3, atgatl6

82 We recovered and characterized T-DNA and transposon-tagged knockout alleles of the MCCA

83 light and plastid signals by characterizing T-DNA insertion alleles of genes that are regulated by l

84 We recovered circular T-DNA molecules (T-circles) using a novel plasmid-rescue

85 Cognate T-DNA knock-down lines display reduced DHNA-CoA thioeste

86 or the formation of extrachromosomal complex T-DNA structures that subsequently may integrate.

87 gh double knockdown mutant plants containing T-DNA insertions in both genes are embryonic lethal, und

88 Seeds containing T-DNA insertions in CTF7 exhibit mitotic defects in the

89 strated by the analysis of the corresponding T-DNA insertional lines.

90 RFA complex in tobacco resulted in decreased T-DNA expression, as determined by infection with A. tum

91 encoding histone H2A-1, results in decreased T-DNA integration into the genome of Arabidopsis roots,

92 eased stable transformation due to decreased T-DNA integration.

93 ted T4SS in vitro, we show that Osa degrades T-DNA in the T-DNA-VirD2 complex before its translocatio

94 m, enter the nucleus, and eventually deliver T-DNA to plant chromatin.

95 wth attenuation in plants of a transfer DNA (T-DNA) insertion mutant of WRKY70 (wrky70) suggest that

96 een a collection of individual transfer DNA (T-DNA) insertion mutants.

97 revealed that double-stranded transfer DNA (T-DNA) intermediates can serve as substrates by as yet u

98 The integration of transferred DNA (T-DNA) and the formation of complex insertions in the ge

99 arious plants by delivering transferred DNA (T-DNA) and virulence proteins into host plant cells.

100 nts by transferring a region of plasmid DNA, T-DNA, into host plant cells.

101 an oncogenic piece of DNA (transferred DNA, T-DNA) into plant cells at the infection site.

102 Although single or double T-DNA mutant tcp8, tcp14 or tcp15 lines were not more su

103 evidence suggests that double-stranded (ds) T-DNA, converted from T-strands, are potent substrates f

104 hat of wild-type plants, suggesting enhanced T-DNA integration in these mutants.

105 Etiolated T-DNA insertion mutants were screened for red fluorescen

106 Here, we show that complex extrachromosomal T-DNA structures form in A. tumefaciens-infected plants

107 200 times higher than the JBP1 affinity for T-DNA.

108 ired for the assembly of the T-pilus and for T-DNA secretion.

109 na NHEJ genes, and by biochemical assays for T-DNA integration.

110 Plants homozygous for T-DNA disruption alleles of GCN5 (encoding a histone ace

111 hromosome, providing greater opportunity for T-DNA to integrate.

112 s for two additional rice varieties and four T-DNA tagged transformants from the Taiwan Rice Insertio

113 Loss-of-function T-DNA insertion siz1-2 and siz1-3 mutations caused ABA h

114 Thermotolerance assays of PME gene T-DNA insertion lines revealed two null mutant alleles o

115 ss the functional significance of the genes, T-DNA mutants were identified.

116 N. benthamiana and Arabidopsis thaliana GOX T-DNA insertion mutants are compromised for nonhost resi

117 Homozygous T-DNA insertion lines are recovered for BRIZ1 and BRIZ2

118 Homozygous T-DNA insertion lines were thus obtained for five of the

119 We phenotyped multiple homozygous T-DNA A. thaliana mutants at each of two loci (AT1G11060

120 We identified T-DNA insertion alleles disrupted in five of the nine co

121 Here we identified T-DNA insertion mutants in three alpha subunit genes (al

122 er we examine callose deposition patterns in T-DNA insertion mutants (cs7) of the Callose Synthase 7

123 binding protein, likely plays a key role in T-DNA nuclear targeting.

124 eased stable transformation due to increased T-DNA integration.

125 Three independent T-DNA insertional mutant lines deficient in 6-phosphofru

126 In contrast, two independent T-DNA knockout lines disrupting AMR1 accumulated 2- to 3

127 ons of Arabidopsis thaliana sequence-indexed T-DNA insertion mutants are among the most important res

128 associated with the use of sequence-indexed T-DNA mutants are described, including the need to genot

129 Analysis of an insertional T-DNA mutant within the REME1 coding sequence confirmed

130 so developed inexpensive methods for INTACT, T-DNA insertion mapping, and profiling of the complete n

131 Integrated T-DNA molecules typically exhibit deletions at their 3'

132 iguration and genomic position of integrated T-DNA molecules likely affect transgene expression, and

133 T-DNA mutants we observed were all intronic T-DNA mutants and the T-DNA fragments in both the trigge

134 demonstrate that the suppression of intronic T-DNA mutants is mediated by trans-interactions between

135 k shows that caution is needed when intronic T-DNA mutants are used.

136 itates proteasomal uncoating of the invading T-DNA from its associated proteins.

137 ucosinolate profile of an Arabidopsis ipmdh1 T-DNA knock-out mutant could be restored to wild-type le

138 ositol synthesis in Arabidopsis, we isolated T-DNA knockout lines of VTC4 that exhibit small perturba

139 f the host cell, Agrobacterium transfers its T-DNA--as a complex (T-complex) with the bacterial VirE2

140 tterns include illegitimate DNA end joining, T-DNA truncations, T-DNA repeats, binary vector sequence

141 We detected JCV large T DNA by quantitative PCR of archival brain samples of 9

142 Launching T-DNA from the picA locus of the Agrobacterium chromosom

143 Analysis of LDIP T-DNA knockdown and knockout mutants showed a decrease i

144 Arf8-6 is a SALK line T-DNA insertion mutant, a class of mutations prone to in

145 using either artificial microRNAs or a LOG2 T-DNA insertion.

146 ation of a new tomato (Solanum lycopersicum) T-DNA mutant allowed for the isolation of the CALCINEURI

147 The utility of many T-DNA insertion mutant lines of Arabidopsis is compromis

148 Arabidopsis reference genome sequence to map T-DNA flanking sequence tags (FST) for over 325,000 T-DN

149 Arabidopsis thaliana lines containing mapped T-DNA insertions have become an important resource for p

150 The MEF8 T-DNA insertion (mef8) line exhibited reduced editing at

151 a CMP or Ubi promoter, were used to monitor T-DNA insert stability and consistency of transgene enco

152 d Arabidopsis GTG1 and GTG2 and isolated new T-DNA insertion alleles of GTG1 and GTG2 in both Wassile

153 In the nhx5 nhx6 T-DNA insertional knockouts, the precursors of the 2S al

154 ber, and the frequency of integration of non-T-DNA portions of the T-DNA-containing vector (backbone)

155 Analyses of T-DNA insertion mutants for each of these candidate PSS1

156 Analyses of T-DNA insertion victr alleles showed that VICTR is neces

157 Characterization of T-DNA insertion mutant alleles at each AACT locus establ

158 t allows the large-scale characterization of T-DNA insertions into the genome of Brachypodium.

159 ene network modeling and characterization of T-DNA mutants indicated that acyl-activating enzyme enco

160 s are present in a widely-used collection of T-DNA insertion lines, we analyzed 64 independent lines

161 ation event, permitting stable expression of T-DNA-encoded transgenes.

162 ium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir) effector proteins

163 Integration of T-DNA into the plant genome establishes a permanent tran

164 somal replicons or off-target integration of T-DNA or replicon sequences.

165 t facilitate the transfer and integration of T-DNA.

166 ts suggest that a cautious interpretation of T-DNA phenotypes is warranted.

167 Here we show that mature leaves of T-DNA insertion lines with diminished expression of PRX3

168 inhibited the transient expression level of T-DNA and only reduced T-DNA integration by 50% suggests

169 ver, mutation of parp1 caused high levels of T-DNA integration and transgene methylation.

170 al identified target genes by phenotyping of T-DNA mutants.

171 Plants of T-DNA insertion mutants, lacking FUM2, show marked diffe

172 may result from asymmetric polymerization of T-DNA's ends.

173 iews our current knowledge of the process of T-DNA integration and proposes ways in which this knowle

174 signaling, we examined ABA sensitivities of T-DNA mutants of a number of Arabidopsis thaliana DWD ge

175 gation analyses from two independent sets of T-DNA insertion mutants indicate that a double disruptio

176 cted phenomenon of epigenetic suppression of T-DNA insertion mutants in Arabidopsis.

177 We show that epigenetic suppression of T-DNA mutants is not a rare event, but certain structura

178 m attachment to plant cells and transport of T-DNA to the nucleus have been identified, but the T-DNA

179 Several DNA substrates (oncogenic T-DNA and plasmids RSF1010 and pCloDF13) induced the Vir

180 enized loss-of-function bin2 alleles and one T-DNA insertional mutation each for BIN2 and its two clo

181 We observed one T-DNA line with normal pollen that nevertheless had a tr

182 how any decrease in stable transformation or T-DNA integration.

183 Analysis of single and higher-order T-DNA insertion jaz null mutants provided further eviden

184 ree triple mutants harboring non-overlapping T-DNA insertions in cytokinin AHK receptors.

185 of DNAPs, even for perfectly complementary P/T DNA sequences.

186 nd near the coding base of the template in P/T DNA complexes with Klenow fragment (KF) DNAP as the po

187 s coupled to conformational changes in the P/T DNA of the complex that are partially regulated by the

188 ions and the conformational changes in the P/T DNA that occur during template-directed DNA synthesis

189 ct") NTPs and on the local sequence of the P/T DNA.

190 conformational changes in primer-template (P/T) DNA are involved in the selective incorporation of dN

191 Characterization of an independent paf1 T-DNA insertion allele and complementation by PAF1 confi

192 of a portion of its tumor-inducing plasmid (T-DNA) into the plant genome.

193 also aid in understanding virulence protein/T-DNA cytoplasmic trafficking.

194 Plants homozygous for pskr1 T-DNA insertions showed enhanced defense gene expression

195 Finally, pyl6 T-DNA mutant plants show an increased sensitivity to the

196 For a number of QTLs, T-DNA insertion knockout lines could validate assigned c

197 as identified in a Beauveria bassiana random T-DNA insertion library.

198 t expression level of T-DNA and only reduced T-DNA integration by 50% suggests that double-stranded T

199 ter-trap transformation vector that requires T-DNA integration into the plant genome to activate a pr

200 fer to immature embryos using two respective T-DNAs.

201 Here over 20,000 M. robertsii T-DNA mutants were screened in order to elucidate geneti

202 Analysis of rpa1 T-DNA insertion mutants demonstrates that although each

203 alt stress-related genes with available SALK T-DNA mutagenesis lines for phenotypic screening and ide

204 el mutant screen, combining a confirmed SALK T-DNA insertion collection with traditional forward gene

205 of more than 3700 confirmed homozygous SALK T-DNA insertion lines for visible defects under prolonge

206 analyzed 64 independent lines from the Salk T-DNA mutant collection.

207 ilencing induced by the dcl3-1 (SALK_005512) T-DNA insertion mutant line.

208 own promoter and terminator, within the same T-DNA.

209 e used a reverse genetics approach to screen T-DNA insertion mutants corresponding to all 47 of the A

210 We therefore screened T-DNA insertion mutants in these RLKs for root hair defe

211 imental procedures for efficiently screening T-DNA lines for the presence of chromosomal abnormalitie

212 ophysical and structural studies of the 2-Se-T DNAs reveal that the bulky 2-Se atom with a weak hydro

213 Single T-DNA mutants and double artificial microRNA knockdowns

214 -optimized Escherichia coli BirA in a single T-DNA construct.

215 Southern blot analyses indicated a single T-DNA insertion in the mutant, located on chromosome 10.

216 ith impaired pollen development and a single T-DNA insertion in the transcription factor gene bHLH142

217 pproximately 40% for Arabidopsis msr1 single T-DNA insertion mutants and by more than 50% for msr1 ms

218 lection, we phenotypically characterized six T-DNA lines with insertions in genes previously shown in

219 homology-dependent silencing induced by some T-DNA insertion mutant lines is siRNA-mediated.

220 Thus, some T-DNA insertion mutant lines induce 35S promoter homolog

221 Accordingly, homozygous ssSPTa T-DNA mutants were not recoverable, and 50% nonviable po

222 gration by 50% suggests that double-stranded T-DNA intermediates, as well as single-stranded T-DNA, p

223 of the T-complex include the single stranded T-DNA, bacterial virulence proteins (VirD2, VirE2, VirE3

224 A integration, a transferred single-stranded T-DNA is converted into a double-stranded form.

225 NA intermediates, as well as single-stranded T-DNA, play significant roles in the integration process

226 opose that termini of linear double-stranded T-DNAs are recognized and repaired by the plant's DNA do

227 A strong T-DNA insertion allele, ftsHi1-2, caused embryo-lethalit

228 The suppressed T-DNA mutants we observed were all intronic T-DNA mutant

229 e trigger T-DNA as well as in the suppressed T-DNA shared stretches of identical sequences.

230 e-indexed Arabidopsis (Arabidopsis thaliana) T-DNA insertion mutants for a diverse set of phenotypes.

231 Previous reports have indicated that T-DNA insertion lines can have chromosomal translocation

232 Various studies suggest that T-DNA is protected inside host cells by VirE2, one of th

233 The T-DNA insertion mutant of MSRB8 exaggerates HR-associate

234 The T-DNA insertional gain-of-function mutant plant for LTP5

235 The T-DNA knockout mutant hcf222-2 showed a more severe defe

236 rved were all intronic T-DNA mutants and the T-DNA fragments in both the trigger T-DNA as well as in

237 The RNA interference lines and the T-DNA insertional mutant of OsbZIP48 showed seedling-let

238 sing primers specific to the adapter and the T-DNA; sequencing of the PCR products; and identificatio

239 to the nucleus have been identified, but the T-DNA integration step during transformation is poorly u

240 The lesion caused by the T-DNA insertion localizes to the promoter region, result

241 king sequence tags (FSTs) characterizing the T-DNA inserts.

242 racterization of a mutant that contained the T-DNA insertion in the promoter region of the TK1a gene.

243 in/F-box protein VBF pathway and exposes the T-DNA molecule to external enzymatic activity.

244 CR amplification of the regions flanking the T-DNA insert(s) using primers specific to the adapter an

245 Nn F1 offspring selected for the T-DNA develop normally under sterile conditions.

246 the number of unique insertion sites in the T-DNA collection by 21 078, bringing the overall total t

247 ing that disruption of BRIZ1 or BRIZ2 in the T-DNA insertion lines is responsible for the observed ph

248 ter-directed cytokinin oxidase 1 gene in the T-DNA insertion lines reduces the endogenous cytokinin l

249 ve during early stages of development in the T-DNA insertional mutant.

250 vent, but certain structural features in the T-DNA mutants are needed in order for the suppression to

251 itro, we show that Osa degrades T-DNA in the T-DNA-VirD2 complex before its translocation.

252 yses of multiple itb2 alleles, including the T-DNA insertion alleles, showed that the loss of ITB2/AL

253 , we could select double mutants lacking the T-DNA already in the first offspring generation.

254 In the mutant, the T-DNA insertion caused a 40 % rise in transcript levels

255 ransmitted to T2 plants independently of the T-DNA construct.

256 ed opr3, potentially through splicing of the T-DNA containing intron.

257 Detailed characterization of the T-DNA insertion mutants des1-1 and des1-2 has provided i

258 immediately flanking the right border of the T-DNA insertion, which encoded an uncharacterized Broad

259 he right and left borders (RB and LB) of the T-DNA inserts and consists of five steps: extraction and

260 e present an overview of the location of the T-DNA inserts of all lines, with one exception.

261 e regions flanking both the LB and RB of the T-DNA inserts significantly improves FST retrieval and t

262 e presence of a translocation, all 11 of the T-DNA lines showing an abnormal pollen phenotype were fo

263 able to complement the growth defects of the T-DNA lines.

264 This mutant contained a copy of the T-DNA tag inserted at the location where the expression

265 of integration of non-T-DNA portions of the T-DNA-containing vector (backbone) into the genome of Ar

266 Two assays were used to screen the T-DNA lines for translocations: pollen viability and gen

267 DNA gel blot analysis showed that the T-DNA inserts are stable; no transgene rearrangements we

268 our genetic mapping experiments was that the T-DNA junctions on the 5'- and 3'-sides of a targeted ge

269 There the T-DNA is uncoated from its protein components before und

270 induction of ife, which is not linked to the T-DNA present in the line) can result in the duplication

271 indicating that this activity can unmask the T-DNA molecule.

272 ght, heat and aluminum stresses, whereas the T-DNA insertion mutant erf74 and the erf74;erf75 double

273 romosomal translocations associated with the T-DNA insertion site, but the prevalence of these rearra

274 orted into host cells concomitantly with the T-DNA to effectuate transformation.

275 results revealed that exon 5, along with the T-DNA, is removed in this mutant, resulting in a truncat

276 These T-DNA insertion mutants, called fldh-1 and fldh-2, are a

277 We show that this T-DNA configuration produces an inverted repeat transcri

278 o of the lines in our survey displayed this 'T-DNA borders separate' phenomenon.

279 Here, we report characterization of three T-DNA insertional mutants of the gene encoding cytochrom

280 Thus, T-DNA integration does not require known NHEJ proteins,

281 Our data suggest that prior to T-DNA integration, a transferred single-stranded T-DNA i

282 Like pskr1 mutants, a tpst T-DNA insertion line exhibited enhanced MAMP-triggered s

283 Furthermore, a transfer (T)-DNA insertional alg3-T2 mutation, which causes accumu

284 Repeated selfing of pgd2 transfer (T-)DNA alleles yielded no homozygous mutants, although s

285 um tumefaciens strains and five transferred (T)-DNA origins of replication on transformation frequenc

286 and the T-DNA fragments in both the trigger T-DNA as well as in the suppressed T-DNA shared stretche

287 Surprisingly, the bin2bil1bil2 triple T-DNA insertional mutant still responds to BR and accumu

288 gitimate DNA end joining, T-DNA truncations, T-DNA repeats, binary vector sequences, and other unknow

289 over conventional Agrobacterium tumefaciens T-DNA.

290 s mediated by trans-interactions between two T-DNA insertions.

291 l the least complex transgenic loci have two T-DNA copies in an inverted repeat configuration, center

292 When the two T-DNA insertion mutants, yuc1-1 and ag-TD, were crossed

293 Compared to the segregation of uncoupled T-DNAs in conventionally produced progeny, the incorpora

294 Using T-DNA insertion lines, we isolated a novel deletion muta

295 Functional studies using T-DNA insertion mutants reveal that they can act as anti

296 CML38-knockout mutants generated via T-DNA insertion were insensitive to AtRALF1, and simulta

297 e to A. brassicicola and B. cinerea, whereas T-DNA insertion alleles are embryonic lethal, suggesting

298 the past decade, phenotypes identified with T-DNA-induced mutants have played a critical role in adv

299 acid (ABA) but is increased in mutants with T-DNA insertions in the FLDH 5' flanking region.

300 Compared with the wild type, WRKY22 T-DNA insertion mutants wrky22-1 and wrky22-2 had lower

301 Furthermore, the zml1 and zml2 T-DNA insertion lines displayed a high irradiance-sensit