ライフサイエンスコーパス: T. maritima

1 T. maritima (Tm) RNase III catalytic activity exhibits a

2 T. maritima cellobiose-binding protein binds a variety o

3 T. maritima CheC, as well as CheX, dephosphorylate CheY,

4 T. maritima CheW and CheY were both soluble and were eas

5 T. maritima FliN is primarily a dimer in solution, and T

6 T. maritima is unable to grow on myo-inositol as a singl

7 T. maritima RRF also inhibited the E. coli RRF reaction

8 T. maritima TrmE was overexpressed in Escherichia coli a

9 thesized and used to identify a clone from a T. maritima lambda library by using PCR.

10 Here, we present the crystal structure of a T. maritima cellobiose-binding protein (tm0031) that is

11 yeast, B. stearothermophilus, T. brucei and T. maritima PGK, and may therefore have a role in the in

12 the interaction between T. maritima CheR and T. maritima MCPs is of relatively low affinity.

13 Nevertheless, both E. coli and T. maritima ExoVII share a similar putative active site

14 to published data for DHFR from E. coli and T. maritima shows a decreasing trend in efficiency of hy

15 a FliN is primarily a dimer in solution, and T. maritima FliN and FliM together form a stable FliM(1)

16 tions differ substantially between yeast and T. maritima.

17 ficient methylation, the interaction between T. maritima CheR and T. maritima MCPs is of relatively l

18 Conservation of gene order between T. maritima and Archaea in many of the clustered regions

19 Here we show that MreB from both T. maritima and E. coli binds directly to cell membranes

20 nated products can be further metabolized by T. maritima in a previously uncharacterized SAH degradat

21 xpressed in Escherichia coli from the cloned T. maritima RRF gene and purified.

22 formational differences between the E. coli, T. maritima, and yeast synthetases suggest the possibili

23 Of the Eubacteria sequenced to date, T. maritima has the highest percentage (24%) of genes th

24 ction for IscU-type proteins, we demonstrate T. maritima IscU-mediated reconstitution of human apofer

25 in the cytoplasmic domains of four different T. maritima chemoreceptors.

26 Beyond the information obtained for T. maritima, the present study illustrates how expressio

27 The operon organization for T. maritima POR was porGDAB.

28 r RRF activity and therefore responsible for T. maritima RRF inhibition of the E. coli RRF reaction.

29 nine and L-allo-threonine are substrates for T. maritima TA, enzymatic assays revealed a strong prefe

30 cherichia coli in complex with RNAP and from T. maritima solved free in solution.

31 Thermotoga sp. RQ2 differs from T. maritima in its genes involved in myo-inositol metabo

32 fied recombinant tagaturonate epimerase from T. maritima was directly confirmed and kinetically chara

33 es from P. furiosus and of the POR gene from T. maritima, all of which comprise four different subuni

34 We cloned the MpgII genes from T. maritima and from Aquifex aeolicus and found that bot

35 s of the putative laminarinase, Lam16A, from T. maritima comprise a highly thermostable family 4 CBM

36 osphate transport system regulator PhoU from T. maritima (a 235-residue mainly alpha-helical protein)

37 ydrogenase that was previously purified from T. maritima does not use either reduced ferredoxin or NA

38 occus jannaschii resulted in fivefold higher T. maritima cell densities when compared with monocultur

39 troglodytes, 9-12 in T. caudata and 10-14 in T. maritima, with some colonies having individuals of mo

40 8, 118 to 122, 154 to 160, and 172 to 176 in T. maritima RRF differed totally from that of E. coli RR

41 CheA binds CheY with identical affinity in T. maritima and E. coli at the vastly different temperat

42 ated during chloramphenicol challenge and in T. maritima bound in exopolysaccharide aggregates during

43 ng a pathway for polysaccharide formation in T. maritima, these results point to the existence of pep

44 at Lys81 (equivalent to Lys150 and Lys82 in T. maritima) for the Bacillus subtilis enzyme suggesting

45 ns and MCPs indicate that MCP methylation in T. maritima occurs independently of a pentapeptide-bindi

46 wide ranging collection of such networks in T. maritima suggests that this organism is capable of ad

47 GK-II in all bacteria and, in particular, in T. maritima.

48 The novel catabolic pathway in T. maritima starts as the conventional route using the m

49 acturonate to mannonate catabolic pathway in T. maritima was reconstituted in vitro using a mixture o

50 uence for chemoreceptor methylation sites in T. maritima that is distinct from the previously identif

51 ng a putative signaling peptide and tmRNA in T. maritima is intriguing, since this overlapping arrang

52 ge in oligomeric state occurs in full-length T. maritima FliG, as well.

53 high structural homology of their monomers, T. maritima MreB and actin filaments display different a

54 In vitro and in vivo analyses of mutant T. maritima and Escherichia coli RodZ validate the struc

55 f WT and mutated (NMBD deletion or mutation) T. maritima CopA, comparing it with Archaeoglobus fulgid

56 The activity of T. maritima DAHP synthase is inhibited by two of the thr

57 , are essential for the nuclease activity of T. maritima ExoVII.

58 Transcriptional analysis of T. maritima cells from these aggregates using a whole ge

59 occludes approximately 35 bp, association of T. maritima HU with DNA of sufficient length to accommod

60 In the case of T. maritima, a three-step serine degradation pathway was

61 terize the molten globule characteristics of T. maritima IscU by near-ultraviolet circular dichroism,

62 Expression of T. maritima RRF inhibited growth of the E. coli host in

63 A specific feature of T. maritima CopA is ATP utilization in the absence of co

64 Growth of T. maritima on monosaccharides was found to be slower th

65 nding to the conserved C-terminal portion of T. maritima FliF.

66 rectly confirmed for the purified product of T. maritima gene dipA cloned and expressed in Escherichi

67 Furthermore, the biophysical properties of T. maritima MreB filaments, including high rigidity and

68 Partial purification of T. maritima proteins was achieved by heat denaturation o

69 d to infer the phylogenetic relationships of T. maritima, one of the deepest-branching eubacteria kno

70 The transcriptional response of T. maritima to specific carbohydrate growth substrates i

71 pporting the reticulate origin of samples of T. maritima in southwestern France and T. sinuatocollis/

72 Here, we present the crystal structure of T. maritima SecA in isolation, determined in its ADP-bou

73 The crystal structures of T. maritima CheC and CheX reveal a common fold unlike th

74 At the optimal growth temperature of T. maritima, MreB assembly proceeded much faster than th

75 these RRFs was less pronounced than that of T. maritima RRF.

76 Recombinant T. maritima proteins, truncated HpkA lacking the putativ

77 The results indicate that the recombinant T. maritima two-component proteins overexpressed in E. c

78 are nine Thermotoga strains to the sequenced T. maritima MSB8.

79 b-families based on EDTA resistance and that T. maritima ExoVII is the first member of the branch tha

80 al denaturation experiments demonstrate that T. maritima IscU is a thermally stable protein with a th

81 Despite the fact that T. maritima has been phylogenetically characterized as a

82 We find that T. maritima FliG(N) is homodimeric in the absence of the

83 The results indicate that T. maritima relies extensively on ABC transporters for c

84 rcular dichroism spectroscopy indicates that T. maritima endonuclease IV has secondary structure simi

85 rophoretic mobility shift analyses show that T. maritima HU (TmHU) binds double-stranded DNA with hig

86 hroism studies of the D40A protein show that T. maritima IscU coordinates a [2Fe-2S]2+ cluster.

87 These observations suggest that T. maritima DAHP synthase is a metalloenzyme.

88 We suggest that T. maritima HU serves an architectural function when ass

89 The T. maritima endonuclease IV gene encodes a 287-amino-aci

90 The T. maritima enzyme exhibits enzyme activity at both low

91 The T. maritima UDG gene has a low level of homology to the

92 sed on cumulative GC skew analysis, both the T. maritima and T. neapolitana lineages contain one or t

93 AdoMet-DCs are structurally conserved in the T. maritima AdoMetDC despite very limited primary sequen

94 carbohydrate-active proteins encoded in the T. maritima genome was followed using a targeted cDNA mi

95 was generated based on the structures of the T. maritima and human AdoMetDCs.

96 reduces the subunit dissociation rate of the T. maritima CheA dimer by interacting with the regulator

97 ike genes are clustered in 15 regions of the T. maritima genome that range in size from 4 to 20 kilob

98 zed proteins define a distinct subset of the T. maritima proteome.

99 ions for 432 proteins, comprising 23% of the T. maritima proteome.

100 A new crystal structure of the T. maritima Psi55S Y67F mutant in complex with a 5FU-RNA

101 Sequence comparisons establish that the T. maritima and Slr0387 proteins have loops of similar l

102 that of E. coli endonuclease IV and that the T. maritima endonuclease IV structure is more stable tha

103 These findings suggest that the T. maritima UDG is a member of a new class of DNA repair

104 as the fold of sigma1.1 from the thermophile T. maritima is distinctly different.

105 Numerous improvements to T. maritima genome annotations were proposed, including

106 commodating RNAP in the DNA channel, whereas T. maritima sigma1.1 must be rearranged to fit therein.