ライフサイエンスコーパス: T. thermophilus

1 T. thermophilus EF-Ts functions only as a homodimer.

2 T. thermophilus HB8 RNA polymerase (RNAP) recognizes mid

3 T. thermophilus Kt-23 has two further non-Watson-Crick b

4 T. thermophilus NDH-1 contains at most nine putative iro

5 T. thermophilus showed no such transition within the tem

6 ed the three proteins with 8His tags using a T. thermophilus expression system.

7 Paradoxically, the dimeric Arg-to-Ala T. thermophilus mutant enzyme packs as a hexamer in the

8 s of three primary proteins from E. coli and T. thermophilus 30S subunits that bind early in the asse

9 E. coli and T. thermophilus have evolved very different mechanisms f

10 rather similar dnaX sequences in E. coli and T. thermophilus lead to very different mechanisms of exp

11 For the E. coli and T. thermophilus proteins, strong agreement is obtained b

12 ults of the binding of RecA from E. coli and T. thermophilus show adaptation to pressure and temperat

13 of labeled membranes of P. denitrificans and T. thermophilus established photoaffinity labeling of th

14 on the crystal structures of human DIPP1 and T. thermophilus Ndx1, were generated using homology mode

15 In the absence of ATP, T. thermophilus RuvB protein bound to linear double-stra

16 bility of key translation components between T. thermophilus and E. coli, and the functional conserva

17 did not inhibit transcription initiation by T. thermophilus RNAP in vitro or shorten the lifetimes o

18 Methylation of 30S ribosomal subunits by T. thermophilus KsgA is more efficient at low concentrat

19 ia coli catabolite activator protein (CAP)], T. thermophilus RNAP sigma(A) holoenzyme, a class II TAP

20 e lifetimes of promoter complexes containing T. thermophilus RNAP, in contrast to the conclusion in t

21 : Lys+Arg is 18.7% for E. coli and 21.2% for T. thermophilus.

22 acting residues: 39% for E. coli and 46% for T. thermophilus.

23 wider range of pressure and temperature for T. thermophilus compared to E. coli RecA, suggesting a c

24 ell below the minimal growth temperature for T. thermophilus.

25 ing the physiological growth temperature for T. thermophilus.

26 eratures, the heat capacity of unfolding for T. thermophilus RNase H is lower, resulting in a smaller

27 nus of its bacterial homolog (subunit C from T. thermophilus) stabilized the yeast subunit d mutant 3

28 elenomethione-substituted apical domain from T. thermophilus was determined to a resolution of 1.78 A

29 nd characterized the NO reductase (NOR) from T. thermophilus.

30 y crystal structures of PRODH and P5CDH from T. thermophilus, a model was built for a proposed PRODH-

31 m18-negative tRNA with recombinant trmH from T. thermophilus abolished its IFN-alpha inducing potenti

32 ure of the dimerization domain of EF-Ts from T. thermophilus refined to 1.7 A resolution.

33 In T. thermophilus ribosomal frameshifting is not required:

34 t of N-terminal PilQ deletion derivatives in T. thermophilus HB27.

35 e identified a novel transcription factor in T. thermophilus and T. aquaticus that shares a high degr

36 zation of protein translation and folding in T. thermophilus.

37 Of these, 20 were found in T. thermophilus 16 S rRNA, 44 in H. marismortui 23 S rRN

38 Inactivation of the tlyA gene in T. thermophilus does not affect its sensitivity to capre

39 ion of long range mechanochemical motions in T. thermophilus leucyl-tRNA synthetase.

40 ate and spectrum of spontaneous mutations in T. thermophilus resembled those of the thermoacidophilic

41 ralogous maltose transport operon present in T. thermophilus.

42 the shuttle vector's ability to replicate in T. thermophilus.

43 mulated over 20 single-base substitutions in T. thermophilus 16S and 23S rRNA, in the decoding site a

44 Instead, T. thermophilus relies on the high temperatures of its e

45 s of new chimeric proteins reveals that like T. thermophilus RNase H, the folding core of C. tepidum

46 ibute to an overall reduction in activity of T. thermophilus ribonuclease H compared to its mesophili

47 stimulate the intrinsic cleavage activity of T. thermophilus RNA polymerase, and increase the k(app)

48 cterizing the intrinsic cleavage activity of T. thermophilus RNA polymerase, we have identified, clon

49 imentally observed conformational changes of T. thermophilus leucyl-tRNA synthetase upon substrate bi

50 e PutA PRODH domain, the FAD conformation of T. thermophilus PRODH is remarkably different and likely

51 The mutation in the hydrophobic core of T. thermophilus IPMDH resulted in a cavity of 32 A3, but

52 -like particles that contain trimers each of T. thermophilus DnaK, DnaJ, and DafA.

53 Also, the FAD of T. thermophilus PRODH is highly solvent-exposed compared

54 To test whether the homodimeric form of T. thermophilus EF-Ts is necessary for catalyzing nucleo

55 Affinity isolation of T. thermophilus HB8 RNAP from P23-45-infected cells iden

56 t in the genomes of the closest relatives of T. thermophilus.

57 clear evidence that the pilus structures of T. thermophilus are not essential for natural transforma

58 sequencing as known hydrophilic subunits of T. thermophilus complex I.

59 idum RNase H is more restricted than that of T. thermophilus.

60 gion and comparisons with similar studies on T. thermophilus RNase H, identify new residues involved

61 ganism E. coli and the thermophilic organism T. thermophilus.

62 domain) of ba(3)-type cytochrome c oxidase (T. thermophilus) (pI = 6.0) exhibit optimal voltammetric

63 in vitro methylation by cloned and purified T. thermophilus PrmA.

64 Finally, we demonstrate that T. thermophilus PRODH reacts with O(2) producing superox

65 ion complex provide compelling evidence that T. thermophilus RNA polymerase can bind to DNA containin

66 In particular, we find that T. thermophilus CuA assembles more rapidly than reported

67 These results indicate that T. thermophilus RNase H is stabilized in a delocalized f

68 ant shows no growth defects, indicating that T. thermophilus PrmA, like its E. coli homolog, is dispe

69 MALDI-TOF MS also revealed that T. thermophilus L11 contains a total of 12 methyl groups

70 a broad phylogenetic range, suggesting that T. thermophilus may be an ideal model system for the stu

71 the UV-sensitive phenotype, suggesting that T. thermophilus RuvB protein has a function similar to t

72 This evidence suggests that T. thermophilus RNAP possesses less intrinsic binding en

73 The T. thermophilus analogs of Arg103, Asn106, Thr134, and L

74 The T. thermophilus NQO2 subunit displayed much higher stabi

75 The T. thermophilus NQO2 subunit was expressed in Escherichi

76 Like other characterized cNORs, the T. thermophilus cNOR consists of two subunits, NorB and

77 ition of ATP or gamma-S-ATP destabilized the T. thermophilus RuvB-DNA complexes.

78 We determined the T. thermophilus V/A-ATPase structure by cryo-EM at 6.4 A

79 within the dimer interface that disrupt the T. thermophilus EF-Ts dimer but not the tertiary structu

80 ithin 3-4 A of the ppGpp binding site in the T. thermophilus cocrystal.

81 e is inserted after position 80 to mimic the T. thermophilus protein reproduce the differences in con

82 teins, relative binding free energies of the T. thermophilus 30S proteins to the 16S RNA were studied

83 deled on the known crystal structures of the T. thermophilus acyl-CoA synthetase with remarkably high

84 The thermal stability of the T. thermophilus apical domain (Tm>100 degrees C as evalu

85 te that mutations increasing activity of the T. thermophilus enzyme at mesophilic temperatures do so

86 e basis of the higher thermostability of the T. thermophilus enzyme.

87 Here we report the structure of the T. thermophilus Gfh1 at 2.4 A resolution revealing a two

88 In the X-ray crystal structure of the T. thermophilus HB8 30 S subunit, the mutated residues a

89 es, we have developed an atomic model of the T. thermophilus ribosome using a homology modeling appro

90 In accordance, we find that growth of the T. thermophilus strain with an inactivated C1942 methylt

91 mparing the membrane-embedded regions of the T. thermophilus V/A-ATPase and eukaryotic V-ATPase from

92 These results strongly suggest that the T. thermophilus NDH-1 is similar to other NDH-1 enzyme c

93 With the advantage of thermostability, the T. thermophilus NDH-1 provides a great model system to i

94 of the antibiotic telithromycin bound to the T. thermophilus ribosome reveals a lactone ring with a c

95 Unlike the T. thermophilus transamidosome, the archaeal complex doe

96 rganisms, and the contacts observed with the T. thermophilus ribosome are consistent with biochemical

97 at there may be multiple pathways within the T. thermophilus cNOR.

98 riophage PH75, which infects the thermophile T. thermophilus, assembles in vivo at 70 degrees C and i

99 e factor GreA from the extreme thermophiles, T. thermophilus and Thermus aquaticus.

100 Expression of thermostable T. thermophilus RuvB protein in the E. coli ruvB recG mu

101 e-stranded DNA endonuclease activity of this T. thermophilus domain is activated not by magnesium but

102 e X-ray crystal structure revealed that this T. thermophilus glucose binding protein (ttGBP) is struc

103 rily found in mesophilic bacteria related to T. thermophilus.

104 thermore, the interdomain angle of wild-type T. thermophilus goes from 81 degrees to 118 degrees wher