ライフサイエンスコーパス: T4SS

1 T4SS foci likely use an existing helical scaffold during

2 T4SS function and cagY in H pylori from C57BL/6 mice wer

3 T4SS proteins expressed by in vitro transcription and tr

4 T4SSs are versatile systems that can transport not only

5 Using a bioinformatics-guided approach, 234 T4SS candidate substrates were identified.

6 c island ICEHin1056 has been identified as a T4SS involved in the propagation of genomic islands.

7 in a loss of phenotypic traits provided by a T4SS.

8 The pKM101 plasmid encodes a T4SS similar to the well-studied model systems from Agro

9 COG-dependent trafficking via delivery of a T4SS effector to promote rBCV biogenesis and intracellul

10 icroscopy structure of the core complex of a T4SS.

11 f cell-autonomous host immunity and reveal a T4SS-translocated L. pneumophila phytase that counteract

12 there was a lack of inflammasome activation, T4SS-dependent cytokine responses, and robust type I int

13 of this study was to determine if additional T4SS proteins are immunogenic for animals immunized with

14 Recent studies on the Agrobacterium T4SS have revealed new information on the localization a

15 The Agrobacterium T4SS is composed of 12 virulence proteins and delivers i

16 VirB8 that is an essential component of all T4SSs.

17 t are required for both pilus biogenesis and T4SS function and reveal that these processes can be unc

18 as one operon, indicating the ribA gene and T4SS operon 1 are co-regulated by both wBmxR1 and wBmxR2

19 s strain, infectious dose, growth phase, and T4SS dependent.

20 Accordingly, antiapoptotic T4SS effector proteins, like AnkG, have been identified.

21 ave not been assessed for their potential as T4SS substrates.

22 train JR32 contains two virulence-associated T4SSs, the Dot/Icm and Lvh T4SSs.

23 omparison of the requirement for a bacterial T4SS in plant versus animal host invasion suggests an im

24 critical role of host TNF and the bacterial T4SS in pathogenesis.

25 To assess relevance to human beings, T4SS function and cagY recombination were assessed in st

26 agM, and cag3 mutants were defective in both T4SS function and pilus formation; complemented mutants

27 Two plasmids encoded both T4SSs, whereas six other plasmids carried none or only a

28 tic protein CD98hc as a partner for Brucella T4SS subunit VirB2.

29 Although the importance of Brucella T4SS is clear, little is known about its interactions wi

30 as novel protein substrates of the Brucella T4SS.

31 ime that the H. pylori cancer-associated cag T4SS is required for TLR9 activation and that H. pylori

32 lori DNA is actively translocated by the cag T4SS to engage this host receptor.

33 12 protein represses the activity of the cag T4SS, as evidenced by the gastric cell "hummingbird" phe

34 that S100A12 represses biogenesis of the cag T4SS.

35 H. pylori and its cag-T4SS exploit alpha5beta1 integrin as a receptor for CagA

36 carry the cag type IV secretion system (cag-T4SS) to inject the cytotoxin-associated antigen A (CagA

37 ted by beta1 integrin interaction of the cag-T4SS.

38 eukaryotic features are considered candidate T4SS substrates.

39 The best characterized T4SS are the type IVA systems, which exhibit extensive s

40 acellular replication, we isolated 20 clonal T4SS substrate mutants using the Himar1 transposon and t

41 rack intracellular replication, we confirmed T4SS-dependent intracellular growth of B. neotomae in ma

42 system (T4SS), a relative of the conjugative T4SS, we demonstrate that catalytically active Osa block

43 Conjugative T4SSs comprise 12 proteins named VirB1-11 and VirD4 that

44 s and found that the four operons containing T4SS genes are transcribed at very different levels.

45 nd post-translational mechanisms controlling T4SS gene expression and DNA secretion.

46 rosophila TNF homolog Eiger and the Coxiella T4SS are implicated in the pathogenesis of C. burnetii i

47 Protein substrates of the Coxiella T4SS are predicted to facilitate the biogenesis of a pha

48 asmic tails, VirB8 and VirD4, or cytoplasmic T4SS substrate proteins, VirD2, VirE2, and VirF, localiz

49 irB9 to assembly and function of the VirB/D4 T4SS.

50 identified 'core' components of the VirB/D4 T4SS.

51 study of type IV secretion system-dependent (T4SS) pathogenesis in the Brucella genus.

52 s, were shared among plasmids from different T4SS groups.

53 dies indicate that substrates of the Icm/Dot T4SS are translocated to the host cytosol in an unfolded

54 n motor to extract pilins from the IM during T4SS biogenesis.

55 e genetic elements and chromosomally encoded T4SS from G+ and G- bacteria, we propose a new classific

56 abolished functionality of the pCF10-encoded T4SS as monitored by pCF10 conjugative transfer.

57 lts suggest that C. burnetii plasmid-encoded T4SS substrates play important roles in subversion of ho

58 nserved among those bacteria also expressing T4SS.

59 intracellular bacterial pathogens expressing T4SSs as well as in many slow-growing soil and aquatic b

60 gh the secretion ATPase DotB is critical for T4SS function, its specific role in type IV secretion re

61 he cag PAI that are known to be required for T4SS function and investigated whether these genes were

62 and the adjacent vir genes predicted to form T4SS, in addition to the status of cag pathogenicity isl

63 ding direct genetic evidence of a functional T4SS in C. burnetii.

64 he absence of thymidine but has a functional T4SS, resisted clearance in G. mellonella up to 18 h pos

65 Unlike other gonococcal T4SS proteins we have investigated, protein levels of th

66 These results suggest that the gonococcal T4SS may be present in single copy per cell and that sma

67 imilar to other F-like T4SSs, the gonococcal T4SS requires a putative membrane protein, TraG, for DNA

68 showed that in contrast with the gonococcal T4SS, the meningococcal T4SS does not secrete DNA, nor d

69 Using the m-ARIA, significantly higher T4SS and VAS scores were obtained when comparing severe

70 Proteins secreted by the Dot/Icm T4SS are presumed to alter the host endocytic pathway, a

71 The Legionella pneumophila Dot/Icm T4SS injects approximately 300 protein effector proteins

72 ocated by the Legionella pneumophila Dot/Icm T4SS into host cells.

73 ly or indirectly in translocation of Dot/Icm T4SS protein substrates.

74 9-fold increases in translocation of Dot/Icm T4SS substrates, LegS2/SplY and LepB.

75 f Legionnaires' disease, employs the Dot/Icm T4SS to inject a large number of protein substrates into

76 or amoeba and HeLa cells and loss of Dot/Icm T4SS-mediated contact haemolysis of erythrocytes.

77 ophagosome interactions by using the Dot/Icm T4SS.

78 ed into macrophage-like cells by the Dot/Icm T4SS.

79 e associated with dysfunction of the Dot/Icm T4SS.

80 osely related Legionella pneumophila Dot/Icm T4SS.

81 eins that included components of the Dot/Icm T4SS.

82 Notably, 26 identified T4SS substrates are hypothetical proteins without predic

83 The discovery of novel immunogenic T4SS proteins recognized by outbred individuals with com

84 d from a patient over time showed changes in T4SS function that were dependent on recombination in ca

85 cagY and cagC mutants were defective in T4SS function but retained the capacity for pilus format

86 for pilus formation, and the role of pili in T4SS function is unclear.

87 tion events typically lead to a reduction in T4SS function in mouse and primate models.

88 tions in the GGI, other strains carry intact T4SS genes and may produce functional secretion systems.

89 that Brucella cytotoxicity in macrophages is T4SS dependent.

90 spase-1-dependent pyroptosis may require its T4SS and activation of the TLR-2 and NLRP3 signaling pat

91 . syringae pv. maculicola encoded a type IVA T4SS (VirB-VirD4 conjugative system), whereas 10 PFPs al

92 om P. syringae pv. tomato encoded a type IVB T4SS (tra system).

93 VirB4, 789 aa, is the largest T4SS component, providing a rich source of possible stru

94 Similar to other F-like T4SSs, the gonococcal T4SS requires a putative membrane

95 f dot/icm virulence defects requires the Lvh T4SS and is associated with a >10-fold induction of LpnE

96 ontrast to dot/icm mutants for which the Lvh T4SS was required, reversal for the DeltalpnE or the Del

97 h independent functioning of Dot/Icm and Lvh T4SSs or functional substitution of the Lvh VirD4 protei

98 ulence-associated T4SSs, the Dot/Icm and Lvh T4SSs.

99 bacterial poles, as is consistent with many T4SS substrates being retained on the phagosomal membran

100 A. marginale T4SS proteins VirB2, VirB4-1, VirB4-2, VirB6-1, VirB7, V

101 fine requirements for phage-contact-mediated T4SS activation and phage penetration.

102 with the gonococcal T4SS, the meningococcal T4SS does not secrete DNA, nor does it confer Ton-indepe

103 In wBm, most T4SS genes are contained in two operons.

104 These data lead to a model with multiple T4SSs around the bacterial cell that likely facilitate h

105 e intracellular replication of a B. neotomae T4SS virB4 mutant was rescued and baseline levels of int

106 The C. burnetii NMII T4SS translocates bacterial products into C57BL/6 macrop

107 ulated by coinfection with wild-type but not T4SS mutant L. pneumophila Using confocal microscopy, it

108 lpha in response to T4SS-sufficient, but not T4SS-deficient, L. pneumophila.

109 1 treatment inhibited C. trachomatis but not T4SS-expressing Coxiella burnetii development in a dose-

110 ngle copy per cell and that small amounts of T4SS proteins TraK and TraB are sufficient for DNA secre

111 Deletion of T4SS genes did not affect association with or invasion o

112 elivery, a subset of which are homologues of T4SS genes from Agrobacterium tumefaciens, the majority

113 ted for the design of specific inhibitors of T4SS function.

114 The bipolar localization of T4SS pilus protein VirB2 on the bacterial surface was de

115 Loss of T4SS function and cagY recombination were more pronounce

116 1-/- mice, showed that cagY-mediated loss of T4SS function requires a T-helper 1-mediated immune resp

117 une response in cagY-dependent modulation of T4SS function.

118 utrophils both are the primary recipients of T4SS-translocated effectors and harbor viable L. pneumop

119 dated LC3 levels were elevated regardless of T4SS activity, no p62 turnover was observed during C. bu

120 haracterize the expression and regulation of T4SS genes and found that the four operons containing T4

121 unctions as an immune-sensitive regulator of T4SS function.

122 ubstantial contribution to the repertoire of T4SS component structures and will serve as springboards

123 at C. burnetii encodes a large repertoire of T4SS substrates that play integral roles in host cell su

124 this class are unable to export a subset of T4SS substrates, providing the first evidence for a DotB

125 nd environmental signals to transcription of T4SS genes are increasingly understood, it remains funda

126 is gene cluster represents a novel family of T4SSs involved in propagation of genomic islands.

127 he biogenesis, architecture, and function of T4SSs.

128 mids within each of the four groups based on T4SS genes; however, a number of genes, encoding plasmid

129 ns use type III secretion systems (T3SSs) or T4SSs to inject or translocate virulence proteins into e

130 TraK nor TraB required the presence of other T4SS components for proper localization.

131 B7 homolog) interacts with TraK, as in other T4SSs.

132 derstand how ImaA could be affecting cag PAI T4SS activity at the host cell interface, we utilized th

133 (HP0289) decreases the action of the cag PAI T4SS via tempering the bacterium's interaction with alph

134 y response that was dependent on the cag PAI T4SS; here we extend those findings to show that the ele

135 ntial component of the pCF10-encoded Prg/Pcf T4SS and that its hydrolase domains coordinate their act

136 The L. pneumophila T4SS substrate SdeA has been shown to require the access

137 the well-established Legionella pneumophila T4SS secretion model.

138 ion levels and localization of two predicted T4SS outer membrane proteins, TraK and TraB, in the wild

139 H pylori T4SS function was assessed by measuring CagA translocati

140 ction of GSK-CagA by the Helicobacter pylori T4SS into different cell types was measured via phosphor

141 With a partially reconstituted T4SS in vitro, we show that Osa degrades T-DNA in the T-

142 lus formation; complemented mutants regained T4SS function and the capacity for pilus formation.

143 Brucella pathogenesis and found a remarkable T4SS-dependent interplay between Brucella and Legionella

144 This coevolution of the rvh T4SS and cell envelope morphology is probably driven by

145 cterization of several components of the rvh T4SS, as well as its putative regulators and substrates.

146 le event of ancestral acquirement of the rvh T4SS, likely from a nonalphaproteobacterial origin.

147 faciens, the Rickettsiales vir homolog (rvh) T4SS is characterized primarily by duplication of severa

148 assessed using the Total Four Symptom Score (T4SS), and the severity was evaluated by both ARIA sever

149 In order to achieve this, several T4SS effectors target regulators of membrane trafficking

150 oint to a novel function for these signature T4SS ATPases in mediating early steps of type IV secreti

151 ts suggest that ElpA is a pathotype-specific T4SS effector that influences ER function during C. burn

152 virB encoding the type IV secretion system (T4SS) (n = 36) and in vjbR encoding a LuxR-like regulato

153 s genes encoding a type IV secretion system (T4SS) and a delivered effector, CagA, that becomes tyros

154 hrough the Icm/Dot type IV secretion system (T4SS) and approximately 300 different "effector" protein

155 stinguished by the type IV secretion system (T4SS) encoded and separated into four groups.

156 quire a functional type IV secretion system (T4SS) for virulence.

157 acterium harbors a type IV secretion system (T4SS) highly similar to the Dot/Icm of Legionella pneumo

158 icient in the VirB type IV secretion system (T4SS) in knockout mice.

159 , and it encodes a type IV secretion system (T4SS) involved in DNA release.

160 e that the Coxiella type 4 secretion system (T4SS) is critical for the formation of the Coxiella-cont

161 eat-killed NMII and type 4 secretion system (T4SS) mutant NMII were unable to induce B1a cell death a

162 The Dot/Icm type IV secretion system (T4SS) of Legionella pneumophila is crucial for the patho

163 ly of the Dot/Icm type IVb secretion system (T4SS) of Legionella pneumophila is dependent on correct

164 ted by the Dot/Icm type IV secretion system (T4SS) of several Legionella pneumophila strains.

165 The type IV secretion system (T4SS) of the plant intracellular symbiont Sinorhizobium

166 The Type IV secretion system (T4SS) plays an important role in bacteria-host interacti

167 encodes a Dot/Icm type IV secretion system (T4SS) predicted to deliver to the host cytosol effector

168 virulence related Type IV secretion system (T4SS) proteins revealed over 250 interactions and will p

169 s, including three type IV secretion system (T4SS) proteins, VirB9-1, VirB9-2, and VirB10.

170 mid pCF10 encode a type IV secretion system (T4SS) required for conjugative transfer.

171 H. pylori carry a type IV secretion system (T4SS) responsible for the injection of the oncoprotein C

172 pertussis toxin, a type IV secretion system (T4SS) substrate, are briefly described and a compilation

173 is a specialized type IVB secretion system (T4SS) that delivers effectors essential for intracellula

174 d, which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host cells.

175 nd (GGI) encodes a type IV secretion system (T4SS) that is found in most strains of N. gonorrhoeae.

176 d orf13, encodes a type IV secretion system (T4SS) that is required for intracellular replication and

177 d, which encodes a type IV secretion system (T4SS) that translocates a pro-inflammatory and oncogenic

178 gen uses a Dot/Icm type IV secretion system (T4SS) to deliver effector proteins to the host cytoplasm

179 thogens that use a type IV secretion system (T4SS) to escape host defenses and create a niche in whic

180 tii uses a Dot/Icm type IV secretion system (T4SS) to generate a phagolysosome-like parasitophorous v

181 rget the conserved type IV secretion system (T4SS) to identify conserved, immunogenic membrane protei

182 a uses the Dot/Icm type IV secretion system (T4SS) to replicate inside host cells.

183 gonorrhoeae uses a type IV secretion system (T4SS) to secrete chromosomal DNA into the medium, and th

184 gonorrhoeae uses a type IV secretion system (T4SS) to secrete chromosomal DNA into the surrounding mi

185 mploying a Dot/Icm type IV secretion system (T4SS) to translocate effector proteins that direct the f

186 ts dot/icm-encoded type IV secretion system (T4SS) to translocate effector proteins that promote its

187 umefaciens VirB/D4 type IV secretion system (T4SS) translocates DNA and protein substrates across the

188 cterium VirB/VirD4 type IV secretion system (T4SS) undergoes a structural transition in response to s

189 etween cells via a type IV secretion system (T4SS) whose channel subunits include the VirD4 coupling

190 host cytosol by a type IV secretion system (T4SS) with homology to the Dot/Icm apparatus of Legionel

191 umefaciens VirB/D4 type IV secretion system (T4SS), a relative of the conjugative T4SS, we demonstrat

192 e Brucella abortus type IV secretion system (T4SS), encoded by the virB genes, is essential for survi

193 la spp. requires a type IV secretion system (T4SS), encoded by the virB locus.

194 e Brucella abortus type IV secretion system (T4SS), encoded by the virB operon, is essential for esta

195 encodes CagA and a type IV secretion system (T4SS), induce more severe disease outcomes.

196 nds on its Dot/Icm type IV secretion system (T4SS), which delivers more than 300 effector proteins in

197 cular, the Dot/Icm type IV secretion system (T4SS), which is essential to establish a replication-per

198 osol via a Dot/Icm type IV secretion system (T4SS).

199 y determinant, the type IV secretion system (T4SS).

200 enotypes require a type IV secretion system (T4SS).

201 of the VirB/VirD4 type IV secretion system (T4SS).

202 ted by the Dot/Icm type IV secretion system (T4SS).

203 of the VirB/VirD4 type IV secretion system (T4SS).

204 researchers is the type IV secretion system (T4SS).

205 nce (vir) -induced type IV secretion system (T4SS).

206 nent systems and a type IV secretion system (T4SS).

207 functional dot/icm type IV secretion system (T4SS).

208 nvironment using a type IV secretion system (T4SS).

209 the H. pylori cag type IV secretion system (T4SS).

210 ds on a functional type IV secretion system (T4SS).

211 ctivity of the cag type IV secretion system (T4SS).

212 y island (cag PAI) type IV secretion system (T4SS).

213 redicted to form a type IV secretory system (T4SS) with vir genes around dupA (dupA cluster).

214 udied conjugative type IV secretion systems (T4SS) are of Gram-negative (G-) origin.

215 Type IV secretion systems (T4SS) are utilized by a wide range of Gram negative bact

216 Bacteria use type IV secretion systems (T4SS) to translocate DNA (T-DNA) and protein substrates

217 Type IV secretion systems (T4SSs) are commonly used secretion machineries in Gram-n

218 Type IV secretion systems (T4SSs) are important virulence factors used by Gram-nega

219 Type IV secretion systems (T4SSs) are large multisubunit translocons, found in both

220 ed with bacterial type IV secretion systems (T4SSs) are thought to generate localized lesions in the

221 Type IV secretion systems (T4SSs) are used by various bacteria to deliver protein a

222 tive bacteria use type IV secretion systems (T4SSs) for a variety of macromolecular transport process

223 Type IV secretion systems (T4SSs) mediate horizontal gene transfer, thus contributi

224 DNA elements via type IV secretion systems (T4SSs) to bacterial or eukaryotic target cells.

225 Gram-negative type IV secretion systems (T4SSs) transfer proteins and DNA to eukaryotic and/or pr

226 rulence-associated type 4 secretion systems (T4SSs), the Dot/Icm type 4B (T4BSS) and the Lvh type 4A

227 nsporters, called type IV secretion systems (T4SSs).

228 r, is mediated by type IV secretion systems (T4SSs).

229 The T4SS apparatus spans both membranes of the bacterium and

230 The T4SS is essential for parasitophorous vacuole formation,

231 The T4SS is required for PV expansion and prevention of apop

232 The T4SS translocates effector proteins, or substrates, into

233 The T4SS, composed of a complex of proteins spanning the inn

234 In addition, the T4SS is important in the initial stages of biofilm devel

235 /phosphomannoisomerase (delta manBA) and the T4SS (delta virB).

236 gulate interactions between integrin and the T4SS and thus alter the host inflammatory strength.

237 tural biology of both the relaxosome and the T4SS.

238 he identity of the molecules secreted by the T4SS has remained elusive.

239 pothesized that proteins translocated by the T4SS would be co-regulated with the virB operon.

240 eins are delivered into the host cell by the T4SS, the function of the majority is unknown.

241 nto the in vivo interactions mediated by the T4SS, we compared host responses elicited by B. abortus

242 translocation signal and was secreted by the T4SS, while the N-terminal portion of the protein was no

243 ch allowed us to track cells injected by the T4SS.

244 In the current study, we further defined the T4SS effector repertoire encoded by the C. burnetii QpH1

245 haplotypes further justifies evaluating the T4SS as a potential vaccine candidate for pathogenic bac

246 otypical H. pylori pathogenicity factor, the T4SS, in the up-regulation of miR-155 in BMMs.

247 (-/-) mice, suggesting a requirement for the T4SS to elicit this pathological change.

248 there is not an absolute requirement for the T4SS to mediate persistence of B. abortus in the spleen.

249 ults show that in the murine model host, the T4SS is required for persistence beyond 3 to 5 days afte

250 cagY is an essential gene in the T4SS and has an unusual DNA repeat structure that predic

251 est that VjbR co-ordinates expression of the T4SS and at least two of its secreted substrates.

252 irst report of co-regulation of genes of the T4SS and riboflavin biosynthesis pathway.

253 des the first link between expression of the T4SS apparatus and intracellular survival of gonococci.

254 12 (virB12) encodes an essential part of the T4SS apparatus.

255 are detectable, structural components of the T4SS are present at very low levels, suggestive of uncha

256 hen identified distinct contributions of the T4SS ATPase subunits to the pilin structural organizatio

257 cts as a docking site at the entrance of the T4SS channel and acts in concert with VirD4 and VirB11 t

258 In this study, we examined the role of the T4SS in mediating PV interactions with autophagosomes.

259 n strains and in a strain lacking all of the T4SS proteins.

260 ns interacting with DotF, a component of the T4SS, and (ii) bioinformatic approaches to retrieve cand

261 n proposed to play a role in assembly of the T4SS, retraction of pili and/or export of substrates.

262 the promoter region of several genes of the T4SS.

263 doglycan cell wall to allow insertion of the T4SS.

264 Overall, the T4SS core complex structure is different in both archite

265 These results suggest that the T4SS is associated with Brucella cytotoxicity in macroph

266 5 days after infection and suggest that the T4SS may contribute to evasion of adaptive immune mechan

267 sion of nucleoprotein substrates through the T4SS.

268 s such as VirE2, VirE3, or VirF) through the T4SS.

269 The substrate is then targeted to the T4SS for export into a recipient cell.

270 unexpectedly displayed a fold similar to the T4SS VirB8 proteins from Agrobacterium tumefaciens and B

271 in consisting of beta-lactamase fused to the T4SS-translocated effector RalF, which allowed us to tra

272 When the T4SS was activated 24 h after infection, autophagosome r

273 The vacuole was decorated with the T4SS effector and LCV marker SidC.

274 mostly obscure structure and function of the T4SSs.

275 or host invasion and persistence, unlike the T4SSs of closely related mammalian intracellular pathoge

276 To determine if any of these T4SS substrates are necessary for intracellular replicat

277 tified that regulates translation of a third T4SS operon.

278 This T4SS functions to secrete single-stranded DNA that is ac

279 This T4SS is novel and evolutionarily distant from the previo

280 Seven of 10 mutants with a mutation in this T4SS did not express the type IV secretion pilus.

281 Moreover, the expression of this T4SS was found to be positively regulated by one of its

282 Genes encoding three T4SS-associated, putative hydrolases, Lactococcus lactis

283 ies of VirB11 mutants established that three T4SS-mediated processes, DNA transfer, protein transfer,

284 They report that R17 penetrates only through T4SS channels engaged for delivery of their plasmid carg

285 GFP fusions to T4SS proteins with cytoplasmic tails, VirB8 and VirD4, o

286 n the GGI but instead was directly linked to T4SS structural components in a manner independent of th

287 and p62 localized to wild-type PV but not to T4SS mutant organism-containing phagosomes in human macr

288 factor and interleukin-1alpha in response to T4SS-sufficient, but not T4SS-deficient, L. pneumophila.

289 s of the canonical Agrobacterium tumefaciens T4SS.

290 The stability and steady-state levels of two T4SS structural proteins were affected by a homolog of t

291 s, suggesting an interaction between the two T4SSs in producing Legionella virulence phenotypes.

292 To better understand T4SS function, we analyzed the localization of its struc

293 omologous to the well-studied archetypal vir T4SS of Agrobacterium tumefaciens, the Rickettsiales vir

294 ion of the T pilus, a subassembly of the vir-T4SS composed of processed and cyclized VirB2 (major sub

295 tensive similarity to the Agrobacterium VirB T4SS.

296 bortus uses a type IV secretion system (VirB T4SS) to generate a replication-permissive Brucella-cont

297 Here we show that the VirB T4SS effector BspB contributes to rBCV biogenesis and Br

298 rB8-1, the first gene in operon 1 of the wBm T4SS.

299 ed and replicated within the larvae, whereas T4SS mutants were rapidly cleared.

300 ATPases that are universally associated with T4SSs.