ライフサイエンスコーパス: T7 RNA polymerase

1 ulting from rapid binding of the promoter to T7 RNA polymerase.

2 vitro run-off RNA synthesis is bacteriophage T7 RNA polymerase.

3 (Gly) to glyQS leader RNA generated by phage T7 RNA polymerase.

4 stream of -17 in transcription regulation of T7 RNA polymerase.

5 tochondria that we had engineered to contain T7 RNA polymerase.

6 h transcription by Escherichia coli and then T7 RNA polymerase.

7 siRNAs are siRNAs produced by bacteriophage T7 RNA polymerase.

8 the second resembles the specificity loop of T7 RNA polymerase.

9 nscription under the T7 class II promoter by T7 RNA polymerase.

10 anscription by both RNA polymerase II and by T7 RNA polymerase.

11 cultivated with human cells transfected with T7 RNA polymerase.

12 range of temperature and salinity than does T7 RNA polymerase.

13 ressed in the cytoplasm of cultured cells by T7 RNA polymerase.

14 or transcription of nucleosomal templates by T7 RNA polymerase.

15 in baby hamster kidney cells that expressed T7 RNA polymerase.

16 a new part for this function: a split intein T7 RNA polymerase.

17 ed antibody raised against the C-terminus of T7 RNA polymerase.

18 ngent for Syn5 RNA polymerase as compared to T7 RNA polymerase.

19 us locations within the promoter element for T7 RNA polymerase.

20 increase in fluorescence upon binding to the T7 RNA polymerase.

21 g low concentrations of ribonucleotides than T7 RNA polymerase.

22 in which we repositioned gene 1, coding for T7 RNA polymerase.

23 inant vaccinia virus (MVA-T7) that expressed T7 RNA polymerase.

24 understand the interaction of promoter with T7 RNA polymerase.

25 se is 24 degrees C, much lower than that for T7 RNA polymerase.

26 scherichia coli using pTara as the source of T7 RNA polymerase.

27 y kidney cells that constitutively expressed T7 RNA polymerase.

28 with a vaccinia virus recombinant expressing T7 RNA polymerase.

29 hymena group I intron using a mutant form of T7 RNA polymerase.

30 with a vaccinia virus recombinant expressing T7 RNA polymerase.

31 ro transcription of linearized plasmids with T7 RNA polymerase.

32 in the cytoplasm of mouse OST7-1 cells using T7 RNA polymerase.

33 rmediates during transcription initiation by T7 RNA polymerase.

34 of the mtRNAP closely resembles that of the T7 RNA polymerase.

35 BSRT7-5 cells, which constitutively express T7 RNA polymerase.

36 ducing RNA transcripts of various lengths by T7 RNA polymerase.

37 es by in vitro transcription reactions using T7 RNA polymerase.

38 initially transcribing abortive complexes in T7 RNA polymerase.

39 We demonstrate that T7 RNA polymerase accepts this fluorescent ribonucleosid

40 placing the template strand correctly in the T7 RNA polymerase active site upon promoter melting for

41 nucleic acid scaffolds that, when mixed with T7 RNA polymerase, allow the formation of functional tra

42 thesis using in vitro transcription by phage T7 RNA polymerase allows preparation of milligram quanti

43 protein expression 5- to 10-fold compared to T7 RNA polymerase alone while enhancing reovirus rescue

44 wnian motion and transcription elongation of T7 RNA polymerase along aligned DNA molecules bound to s

45 T7 gene 3.5 that affect its interaction with T7 RNA polymerase, also reduce the interference with T7

46 Transcription by T7 RNA polymerase alters the folding pathway of both RNA

47 5' end of the RNA transcript or SMART) with T7 RNA polymerase amplification.

48 l by combining exponential (PCR) and linear (T7 RNA polymerase) amplification.

49 These catalytic properties make T7 RNA polymerase an ideal tool for synthesizing large f

50 Using T7 RNA polymerase and a cloned template containing the r

51 and RNA-primed DNA "bubble" constructs with T7 RNA polymerase and by initiating transcription at pro

52 ould be generated following transcription by T7 RNA polymerase and cleavage by hepatitis delta virus

53 ned protein systems that minimally contained T7 RNA polymerase and DNA gyrase.

54 cs between single molecules of bacteriophage T7 RNA polymerase and DNA, as a function of tension.

55 rminator protein (Reb1p) was unable to block T7 RNA polymerase and E. coli DnaB helicase.

56 ugates inhibited transcription elongation by T7 RNA polymerase and eukaryotic RNA polymerase II from

57 Here we use T7 RNA polymerase and exonuclease III as probes to obtai

58 ese data show that O(6)-meG partially blocks T7 RNA polymerase and human RNA polymerase II elongation

59 ified O(6)-meG DNA template by bacteriophage T7 RNA polymerase and human RNA polymerase II.

60 Bacteriophage T7 lysozyme binds to T7 RNA polymerase and inhibits transcription initiation

61 an layer of the cell wall, but it also binds T7 RNA polymerase and inhibits transcription, and it sti

62 8azaGTP is an efficient substrate for T7 RNA polymerase and is incorporated specifically oppos

63 The bacteriophage T7 RNA polymerase and its promoters were used in a model

64 of DNA templates, in vitro transcription by T7 RNA polymerase and kit-based purification provides a

65 d cross-links on transcription elongation by T7 RNA polymerase and mammalian RNA polymerase II.

66 their effect on transcription elongation by T7 RNA polymerase and mammalian RNA polymerase II.

67 C3P3, a fusion protein containing T7 RNA polymerase and NP868R, was found to increase prot

68 was due to a combination of mutations in the T7 RNA polymerase and other genes expressed at the same

69 of the analogues is readily incorporated by T7 RNA polymerase and produces fully active transcripts

70 control the expression of both bacteriophage T7 RNA polymerase and recombinant gene(s) of interest.

71 hymine glycol on transcription elongation by T7 RNA polymerase and RNA polymerase II from rat liver.

72 T7 RNA polymerase and site-specifically platinated DNA t

73 scription system was applied, which utilizes T7 RNA polymerase and template DNAs that are either mode

74 d d5SICS:dNaM are selectively transcribed by T7 RNA polymerase and that the efficiency of d5SICS:dNaM

75 e (nA) and 2-thiouracil (sU) are taken up by T7 RNA polymerase and that the resulting RNA possesses r

76 atch and dialysis mode) under the control of T7 RNA polymerase and to other environments where transc

77 Transcription of RNA pools with T7 RNA polymerase and UNH(2) or USH occurred with effici

78 xtracted from these cells was amplified with T7 RNA polymerase and used to hybridize a microarray con

79 tude larger binding constant between DNA and T7 RNA polymerase, and five to six times larger rate con

80 cap analogue was an efficient substrate for T7 RNA polymerase, and the mRNA transcribed, with a poly

81 scription substrate for Escherichia coli and T7 RNA polymerases, and yeast RNA polymerase III.

82 d strand blocked transcription elongation by T7 RNA polymerase approximately 50% of the time but did

83 ter linkages; both Klenow DNA polymerase and T7 RNA polymerase are found to synthesize complementary

84 ng in vitro transcription of longer repeats, T7 RNA polymerase arrests in the promoter distal end of

85 experimental system that uses bacteriophage T7 RNA polymerase as a probe for aspects of nucleosome t

86 he case for phage polymerases, as used here (T7 RNA polymerase), as well as RNA polymerases in bacter

87 etic studies, we propose that a fast form of T7 RNA polymerase binds promoter double-stranded DNA by

88 y shown to increase the thermal tolerance of T7 RNA polymerase can increase the activity of mutants w

89 with a biotin tag, have been prepared using T7 RNA polymerase-catalyzed transcription of synthetic D

90 We determined the crystal structures of T7 RNA polymerase complexes captured during the de novo

91 Transcription initiation as catalyzed by T7 RNA polymerase consists primarily of promoter binding

92 Phage T7 RNA polymerase contains within its single polypeptide

93 rated a fusion protein of the mouse CTD with T7 RNA polymerase (CTD-T7 RNAP).

94 in the thumb subdomain (residues 335-408) of T7 RNA polymerase decrease elongation complex stability

95 from the initial, promoter-bound complex of T7 RNA polymerase describes the very beginning of the in

96 hypernegative supercoiling of plasmid DNA by T7 RNA polymerase did not require anchoring of DNA to th

97 nst a d(tC-A) base pair only by factors <10, T7 RNA polymerase discriminates against tC-A base pair f

98 Based on the recent crystal structure of the T7 RNA polymerase-DNA complex, we propose that the large

99 Remarkably, T7 RNA polymerase does not incorporate tCTP with the sam

100 We established an RVFV T7 RNA polymerase-driven minigenome system in which T7 R

101 M1 was expressed by using the vaccinia virus T7 RNA polymerase-driven overexpression system, in our n

102 We established a T7 RNA polymerase-driven reverse genetics system to resc

103 also made in vivo in uninfected cells when a T7 RNA polymerase-driven transient-transfection system w

104 ady-state conditions and also transcribed by T7 RNA polymerase efficiently in either direction.

105 T7 RNA polymerase elongates RNA at a relatively high rat

106 slippage synthesis, this study reveals that T7 RNA polymerase elongation complexes containing only a

107 Furthermore, NEP and the phage T7 RNA polymerase exhibit similar sensitivity to inhibit

108 Viral RNAs were transcribed by T7 RNA polymerase expressed from recombinant vaccinia vi

109 d in molecular biology applications that use T7 RNA polymerase for in vitro transcription.

110 cription, T7 lysozyme, does not compete with T7 RNA polymerase for RNA cross-linking, implying that t

111 initiation by affecting both the affinity of T7 RNA polymerase for the promoter and the efficiency of

112 During the early stages of transcription, T7 RNA polymerase forms an unstable initiation complex t

113 polymerase-driven minigenome system in which T7 RNA polymerase from an expression plasmid drove expre

114 e sliding and transcription by bacteriophage T7 RNA polymerase from the nucleosomal template, but not

115 tion of the nontemplate strand on release of T7 RNA polymerase from the T7 promoter.

116 e constructed in which the ecdysone promoter-T7 RNA polymerase gene had been integrated intact, as de

117 here that the pET system, in which the phage T7 RNA polymerase gene is expressed via lac operon contr

118 ible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an

119 , in which a lambdaDE3 prophage containing a T7 RNA polymerase gene under the control of lacUV5 promo

120 To assay late gene expression, the T7 RNA polymerase gene was inserted into the genome of t

121 Independent populations of T7 RNA polymerase genes were subjected to one of two sel

122 In transcription studies with T7 RNA polymerase, H3 and H4 were transferred to the nas

123 n alternative and facile delivery system for T7 RNA polymerase has been devised and constructed.

124 hat the high-resolution crystal structure of T7 RNA polymerase has been solved.

125 This biotin-PC GMP, accepted by T7 RNA polymerase, has been used to transcribe RNAs rang

126 Recent studies in the single subunit T7 RNA polymerase have argued against scrunching as the

127 lve non-equivalent mechanisms, as mutants of T7 RNA polymerase have been identified that fail to reco

128 Over the years, a panel of T7 RNA polymerases have been designed or evolved to acce

129 hnique, termed immuno-detection amplified by T7 RNA polymerase (IDAT) that is capable of monitoring p

130 With the linear amplification ability of T7 RNA polymerase, IDAT represents a significant improve

131 the present study the gene for bacteriophage T7 RNA polymerase, implanted with a eukaryotic nuclear l

132 The structures of phage T7 RNA polymerase in an elongation phase substrate compl

133 As of PaV RNAs 1 and 2 were cotranscribed by T7 RNA polymerase in baby hamster kidney cells that expr

134 a single protein having the expected size of T7 RNA polymerase in immunoblots of cell extracts probed

135 rigins contain T7 promoters, but the role of T7 RNA polymerase in initiating replication is not under

136 These results highlight the malleability of T7 RNA polymerase in recognizing its promoter element an

137 nstrates that halted elongation complexes of T7 RNA polymerase in the absence of termination signals

138 nucleosomes, and transcription was done with T7 RNA polymerase in the presence of a negatively coiled

139 Transcription was done with T7 RNA polymerase in the presence of E. coli topoisomera

140 sphate (tCTP) and tested it as substrate for T7 RNA polymerase in transcription reactions, a convenie

141 a coli DNA replication and DnaB helicase and T7 RNA polymerase in vitro in both orientations.

142 the polyprotein and transfected the derived T7 RNA polymerase in vitro transcripts into FRhK-4 cells

143 oncentration, Hlp represses transcription by T7 RNA polymerase in vitro whereas the individual N- and

144 ffects of this structure on transcription by T7 RNA polymerase in vitro.

145 ient, completely inhibiting transcription by T7 RNA polymerase in vitro.

146 this (CG)(14) sequence on transcription with T7 RNA polymerase in vitro.

147 as in defined transcription reactions using T7 RNA polymerase in vitro.

148 be exclusively transcribed by bacteriophage T7 RNA polymerase in vivo.

149 current study strongly supports a model for T7 RNA polymerase in which initial bubble collapse from

150 A T7 RNA polymerase in which Tyr639 is mutated to Phe read

151 When transcribing a guanine, T7 RNA polymerase incorporates tCTP with 2-fold higher c

152 Further, T7 RNA polymerase incorporates ZTP opposite its Watson-C

153 here that transcription by the bacteriophage T7 RNA polymerase increases the deamination of cytosine

154 ce with similar kinetics upon binding to the T7 RNA polymerase, indicating that the TATA sequence bec

155 Crystal structures of T7 RNA polymerase initiation and elongation complexes ha

156 It is proposed that T7 RNA polymerase interacts directly with the Hoogsteen

157 We divide T7 RNA polymerase into two expression domains and fuse e

158 T7 RNA polymerase is an excellent candidate for studying

159 T7 RNA polymerase is known to induce bending of its prom

160 T7 RNA polymerase is one of the simplest RNA polymerases

161 In contrast, nascent pre-mRNA synthesized by T7 RNA polymerase is quantitatively assembled into the n

162 Bacteriophage T7 RNA polymerase is the best-characterized member of a

163 Phage T7 RNA polymerase is the only DNA-dependent RNA polymera

164 is conjugated to an antibody (Ab), and then T7 RNA polymerase is used to amplify RNA from the double

165 The fastidious behavior of T7 RNA polymerase limits the incorporation of synthetic

166 Here we show by using T7 RNA polymerase-mediated production of PV genomic RNA,

167 T7 RNA polymerase mutants that exhibit reduced elongatio

168 ng a modified vaccinia virus which expresses T7 RNA polymerase (MVA-T7).

169 accinia virus MVA encoding the bacteriophage T7 RNA polymerase (MVA/T7).

170 wing expression in a modified vaccinia virus/T7 RNA polymerase (MVA/T7RP) system.

171 We also observe binding and transcription by T7 RNA polymerases on single combed T7 DNA molecules wit

172 removed from RNA substrates transcribed from T7 RNA polymerase or delivered directly to the cytoplasm

173 ally inhibited DNA transcription mediated by T7 RNA polymerase or human RNA polymerase II in vitro an

174 DNA transcription mediated by single-subunit T7 RNA polymerase or multisubunit human RNA polymerase I

175 ing transcription mediated by single-subunit T7 RNA polymerase or multisubunit human RNA polymerase I

176 to examine the fidelity of transcription by T7 RNA polymerase past an adenine residue adducted at th

177 Time-resolved characterization of T7 RNA polymerase pausing and terminating at a class II

178 The structure of T7 RNA polymerase presented here differs significantly f

179 T7 RNA polymerase presents a very simple model system fo

180 reverse transcription with oligo(dT) with a T7 RNA polymerase promoter (T7dT) on the 5' end, and sub

181 y assembling five cDNA fragments between the T7 RNA polymerase promoter and the autocatalytic hepatit

182 st, capsid did not inhibit expression from a T7 RNA polymerase promoter construct, suggesting that th

183 ntrinsic and polymerase-induced bends in the T7 RNA polymerase promoter DNA.

184 The T7 RNA polymerase promoter has been proposed to contain

185 l) contains a non-functional single-stranded T7 RNA polymerase promoter sequence.

186 PLP-1 domain in Escherichia coli by using a T7 RNA polymerase promoter system or as a maltose-bindin

187 plasmid vector directly downstream from the T7 RNA polymerase promoter, and capped RNA transcripts d

188 s NP, P, and L proteins under control of the T7 RNA polymerase promoter, were transfected into A549 c

189 These results suggested that, in the T7 RNA polymerase-promoter complex, the polymerase molec

190 ase of the T7 promoter is inhibited when the T7 RNA polymerase-promoter interaction is strengthened.

191 that open complex formation in bacteriophage T7 RNA polymerase:promoter binary complexes is thermodyn

192 specific stabilizing interactions, of the 17 T7 RNA polymerase promoters in the phage genome, 15 begi

193 translated in HeLa cells when transcribed by T7 RNA polymerase provided by a recombinant vaccinia vir

194 ed from cotransfected plasmids driven by the T7 RNA polymerase provided by the recombinant vaccinia v

195 e synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative t

196 T7 RNA polymerase recognizes a small promoter, binds DNA

197 We found that T7 RNA polymerase recognizes NTPalphaSe Sp diastereomers

198 mid with pTara provides a low-cost method of T7 RNA polymerase-regulated expression that can be fine-

199 o I, bcTopo IIIalpha), have been cloned into T7 RNA polymerase-regulated plasmid expression vectors a

200 T7 RNA polymerase requires a double-stranded DNA promote

201 ins of AMPV/CO, into cells stably expressing T7 RNA polymerase resulted in the recovery of infectious

202 nscription complexes formed by bacteriophage T7 RNA polymerase reveal a nucleotide-addition cycle dri

203 Transcription footprinting using T7 RNA polymerase revealed major single-base damage site

204 Bacteriophage T7 lysozyme binds to T7 RNA polymerase (RNAP) and regulates its transcription

205 We have examined the behavior of T7 RNA polymerase (RNAP) at a set of promoter variants h

206 orm a functional open complex, bacteriophage T7 RNA polymerase (RNAP) binds to its promoter DNA and i

207 Recent work showed that the single-subunit T7 RNA polymerase (RNAP) can generate misincorporation e

208 The region in bacteriophage T7 RNA polymerase (RNAP) comprising residues 421-425 con

209 The high specificity of T7 RNA polymerase (RNAP) for its promoter sequence is me

210 To initiate transcription, T7 RNA polymerase (RNAP) forms a specific complex with i

211 n vitro transcription of the kan gene by the T7 RNA polymerase (RNAP) in the presence of AID and a ge

212 T7 RNA polymerase (RNAP) is able to traverse a variety o

213 Transcription errors by T7 RNA polymerase (RNAP) may occur as the result of a me

214 We have characterized the roles of the phage T7 RNA polymerase (RNAP) thumb subdomain and the RNA bin

215 stability is a kinetic mechanism that allows T7 RNA polymerase (RNAP) to achieve promoter specificity

216 ted a series of plasmid templates that allow T7 RNA polymerase (RNAP) to be halted at defined interva

217 mplex (EC), the single-subunit bacteriophage T7 RNA polymerase (RNAP) undergoes dramatic conformation

218 Using PACE, we evolved T7 RNA polymerase (RNAP) variants that recognize a disti

219 omplex (IC) to an elongation complex (EC) in T7 RNA polymerase (RNAP), we used nucleic acid-protein c

220 acts as a partial block to the bacteriophage T7 RNA polymerase (RNAP), which allows nucleotide incorp

221 pausing and/or termination by bacteriophage T7 RNA polymerase (RNAP).

222 leic acid scaffolds of defined sequence with T7 RNA polymerase (RNAP).

223 Yeast mitochondrial (YMt) and phage T7 RNA polymerases (RNAPs) are two divergent representat

224 template that contains promoters for T3 and T7 RNA polymerases (RNAPs) in opposing orientations, and

225 of bound target by quantifying DNA tails by T7 RNA polymerase runoff transcription and real-time pol

226 T7 RNA polymerase selectively transcribes T7 genes durin

227 this system, we found that transcription by T7 RNA polymerase strikingly induced the formation of hy

228 Comparison of the T7 RNA polymerase structure with that of the homologous

229 reted in the context of our revised model of T7 RNA polymerase, suggest a conserved fold.

230 s in mammalian cells were investigated using T7 RNA polymerase-synthesized small interfering RNA and

231 T7 RNA polymerase synthesizes, in addition to run-off pr

232 We found that siRNAs synthesized from the T7 RNA polymerase system can trigger a potent induction

233 of transcription in the model bacteriophage T7 RNA polymerase system, the simplest possible reaction

234 n transfer RNAPhe (tRNAPhe) transcribed in a T7 RNA polymerase system.

235 The structures of T7 RNA polymerase (T7 RNAP) captured in the initiation a

236 nd single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmod

237 The structure of a T7 RNA polymerase (T7 RNAP) initiation complex captured

238 Transcription initiation by T7 RNA polymerase (T7 RNAP) is regulated by the specific

239 During transcription initiation, T7 RNA polymerase (T7 RNAP) melts specifically the -4 to

240 in vitro transcription system with purified T7 RNA polymerase (T7 RNAP) or rat liver RNAP II, we cha

241 To address this, we modified bacteriophage T7 RNA polymerase (T7 RNAP) to create a library of trans

242 amide linkage were tested as substrates for T7 RNA polymerase (T7 RNAP) transcription.

243 ake messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from

244 sed on co-delivery of mRNA(A64) encoding for T7 RNA polymerase (T7 RNAP) with a T7-driven plasmid, pr

245 l interest, T7 RNA polymerase (T7RP) and the T7 RNA polymerase-T7 lysozyme complex (T7RPL) in forms s

246 The T7 RNA polymerase-T7 lysozyme complex regulates phage ge

247 On the basis of their recently described T7 RNA polymerase-T7 promoter crystal structure, Cheetha

248 Studies of halted T7 RNA polymerase (T7RNAP) elongation complexes (ECs) or

249 We have used bacteriophage T7 RNA polymerase (T7RNAP) to study the transcriptional

250 al4, (ii) transcription by the bacteriophage T7 RNA polymerase (T7RNAP), and (iii) FLP-mediated site-

251 approaches that may be of general interest, T7 RNA polymerase (T7RP) and the T7 RNA polymerase-T7 ly

252 ubstitute was a more effective substrate for T7 RNA polymerase than 5-(2-mercaptoethyl)uridine tripho

253 otein trans-splicing, yielding a full-length T7 RNA polymerase that can transcribe genes via a T7 pro

254 Variants of T7 RNA polymerase that reduce toxicity were constructed

255 ed an unusual type of termination signal for T7 RNA polymerase that requires a conserved 7-base pair

256 re by providing examples of (i) selection of T7 RNA polymerases that recognize orthogonal promoters a

257 In T7 RNA polymerase these changes involve refolding and re

258 Unlike the salt-sensitive bacteriophage T7 RNA polymerase, this marine RNA polymerase requires 1

259 Using T7 RNA polymerase to explore the transcriptional accessi

260 nt translation of viral transcripts, we used T7 RNA polymerase to express constructs engineered with

261 ence functional) by DNA polymerase, allowing T7 RNA polymerase to generate a target-dependent RNA sig

262 The PCRcDNA was in vitro transcribed by T7 RNA polymerase to generate complementary RNA (cRNA),

263 Using bacteriophage T7 RNA polymerase to install poly(G) tails on mRNAs tran

264 rt of the upstream binding energy is used by T7 RNA polymerase to melt the downstream initiation regi

265 Inability of T7 RNA polymerase to processively transcribe higher euka

266 We have used the model T7 RNA polymerase to transcribe reconstituted nucleosome

267 As observed during T7 RNA polymerase transcript elongation, substrate loadi

268 ast 100-fold more efficient than that from a T7 RNA polymerase transcript with the same sequence.

269 ed by these genes were overexpressed using a T7 RNA polymerase transcription (pET102/D-TOPO) system i

270 ese stereoisomers on RNA synthesis, in vitro T7 RNA polymerase transcription assays were performed us

271 the non-template strand reduces the yield of T7 RNA polymerase transcription by more than an order of

272 Here, we photo-cross-linked the RNA in a T7 RNA polymerase transcription complex and mapped a maj

273 aracterize the transitions that occur in the T7 RNA polymerase transcription complex during initiatio

274 ur picture of the functional architecture of T7 RNA polymerase transcription complexes remains incomp

275 e sequences on transcription we have studied T7 RNA polymerase transcription of G-rich sequences in v

276 anslated in vivo after transcription using a T7 RNA polymerase transcription system.

277 We have studied T7 RNA polymerase transcription through the sequence fro

278 For bacteriophage T7 RNA polymerase, transcription begins with a marked pr

279 T7 RNA polymerase undergoes dramatic structural rearrang

280 The N-terminal domain of T7 RNA polymerase undergoes large conformational changes

281 ation complex to an elongation complex (EC), T7 RNA polymerase undergoes major conformational changes

282 Bacteriophage T7 RNA polymerase undergoes major conformational changes

283 ity in a simplified model system, by using a T7 RNA polymerase variant capable of transcribing nuclei

284 We apply CPR to evolve a T7 RNA polymerase variant that recognizes an orthogonal

285 tem also enables the continuous evolution of T7 RNA polymerase variants capable of initiating transcr

286 T7 RNA polymerase was able to bypass all the photoproduc

287 The induced T7 RNA polymerase was exclusively localized in the nucle

288 A caged T7 RNA polymerase was expressed in cells with an expande

289 quence preference in start site selection by T7 RNA polymerase was investigated by using a series of

290 and open complex formation in bacteriophage T7 RNA polymerase was investigated using 2-aminopurine (

291 Transcription driven by phage T7 RNA polymerase was not regulated by cobalamins, altho

292 The induced T7 RNA polymerase was present at numerous punctate foci

293 Here, using a purified in vitro system with T7 RNA polymerase, we show that increased distance betwe

294 Using T7 RNA polymerase, we synthesized the hantavirus S segme

295 Both RNA polymerases I and III, but not T7 RNA polymerase, were inhibited by juglone.

296 strate here that the P266L point mutation in T7 RNA polymerase, which shows dramatically reduced abor

297 lular synthesis of the LCMV MG was driven by T7 RNA polymerase whose expression was also mediated by

298 emplate encoding the initial sequence GGGA., T7 RNA polymerase will synthesize a 'ladder' of poly-G R

299 Further, we found that a mutant T7 RNA polymerase with a slower rate of elongation cause

300 We fluorescently label T7 RNA polymerase with antibodies and use flow to convec