ライフサイエンスコーパス: TAL effector

1 third mechanism of rice resistance involving TAL effectors.

2 ere experimentally proven targets of natural TAL effectors.

3 ays of polymorphic amino acid repeats in the TAL effectors.

4 factors called transcription activator-like (TAL) effectors.

5 et activity of transcription activator-like (TALs) effectors.

6 ription factors in plant cells; however, how TAL effectors activate host transcription is unknown.

7 Plants counter with TAL effector-activated executor resistance genes, which

8 bles prediction of genomic binding sites for TAL effectors and customization of TAL effectors for use

9 nclude a plasmid construct for making custom TAL effectors and one for TAL effector fusions to additi

10 sign guidelines based on naturally occurring TAL effectors and their binding sites.

11 be used to predict binding sites of natural TAL effectors and to design novel synthetic DNA-binding

12 thy lines exhibiting gene activation by each TAL effector, and resistance to PXO99(A) , a PXO99(A) de

13 g the search space for off-targets of custom TAL effectors, and highlighting the potential of TAL eff

14 Transcription activator-like (TAL) effectors are encoded by plant-pathogenic bacteria

15 Transcription activator-like (TAL) effectors are repeat-containing proteins used by pl

16 Xa10 contains a binding element for the TAL effector AvrXa10 (EBEAvrXa10) in its promoter, and A

17 nas oryzae pv. oryzae (Xoo) that deliver the TAL effector AvrXa27.

18 TAL effectors bind DNA on the basis of a unique code tha

19 gene could be broadened by adding different TAL effector binding elements (EBEs) to it.

20 arrays for desired targets and prediction of TAL effector binding sites, ranked by likelihood, in a g

21 e repeat variable di-residues that determine TAL effector binding specificity, and is independent of

22 lending support to the predictive models for TAL effector binding specificity.

23 e, by combining transcriptome profiling with TAL effector-binding element (EBE) prediction, we show t

24 Here, we demonstrate that TAL effectors can drive plant transcription from EBEs on

25 nt, and that sgRNA:Cas9 complexes and 18-mer TAL effectors can potentially tolerate 1-3 and 1-2 targe

26 tion domain of transcription activator-like (TAL) effectors can be combined with the nuclease domain

27 l mechanisms of resistance in plants against TAL effector-containing pathogens have given insights in

28 SWEET-targeting TAL effectors contribute broadly and non-tissue-specific

29 sucrose transporter genes were expressed in TAL effector-deficient X. oryzae strain X11-5A, and asse

30 TAL effectors delivered by phytopathogenic Xanthomonas s

31 es two host genes, CsLOB1 and CsSWEET1, in a TAL effector-dependent manner.

32 ion of Xa10, a transcription activator-like (TAL) effector-dependent R gene for resistance to bacteri

33 mputational model, Specificity Inference For TAL-Effector Design (SIFTED), to predict the DNA-binding

34 sucrose transporter, is induced by Avrb6, a TAL effector determining Xcm pathogenicity.

35 Xoc TAL effectors did not alter X11-5A virulence.

36 Artificially designed TAL effectors directed to sequences in the CsLOB1 promot

37 combinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are a class of reagents

38 ions, the structure illustrates the basis of TAL effector-DNA recognition.

39 ent studies of transcription activator-like (TAL) effector domains fused to nucleases (TALENs) demons

40 Both TAL effectors drive expression of CsLOB1 and CsSWEET1 pr

41 enes oriented head to head, we show that the TAL effector drives expression from either EBE in the re

42 ectors enable custom-engineering of designer TAL effectors (dTALE) for gene activation.

43 Activation of GhSWEET10 by designer TAL effectors (dTALEs) restores virulence of Xcm avrb6 d

44 e modular nature and DNA recognition code of TAL effectors enable custom-engineering of designer TAL

45 Xoo TAL effectors enhanced X11-5A virulence on most varietie

46 tly evolved mechanism to recognize analogous TAL effector epitopes.

47 members of the transcription activator-like (TAL) effector family whose central repeat units dictate

48 members of the transcription activator-like (TAL) effector family.

49 members of the transcription activator-like (TAL) effector family.

50 effectors, and highlighting the potential of TAL effectors for probing fundamental aspects of plant t

51 sites for TAL effectors and customization of TAL effectors for use in DNA targeting, in particular as

52 Furthermore, we show that a native TAL effector from Xanthomonas oryzae pv. oryzicola drive

53 ession of multiple host genes using multiple TAL effectors from a single strain, and evidence support

54 f disease development in response to diverse TAL effectors from both X. oryzae pathovars.

55 sors in sweet orange, our data indicate that TAL effectors from X. citri target negative regulators o

56 Transcription activator-like (TAL) effectors from Xanthomonas citri subsp. malvacearum

57 Transcription activator-like (TAL) effectors from Xanthomonas species pathogens act as

58 for making custom TAL effectors and one for TAL effector fusions to additional proteins of interest.

59 ecies citri strain Xcc306, with the type III TAL effector gene pthA4 or with the distinct yet biologi

60 preventing disease by strains containing the TAL effector gene pthXo1, which directs robust expressio

61 lated gene using any one of a diverse set of TAL effector genes in the pathogen populations.

62 Three additional distinct TAL effector genes, pthA*, pthB, and pthC, also direct p

63 known type III transcription activator-like (TAL) effector genes for the characteristic pustule forma

64 dent on major transcription activation-like (TAL) effector genes, and correlates with reduced express

65 tremely modular DNA binding proteins such as TAL effectors, has generally proved to be quite challeng

66 ne with no corresponding naturally occurring TAL effector identified, conferred susceptibility to the

67 The roles of X. oryzae TAL effectors in diverse rice backgrounds, however, are

68 binding elements (EBEs) recognized by native TAL effectors in plants have been identified only on the

69 e prediction, and opens prospects for use of TAL effectors in research and biotechnology.

70 lity of X11-5A for characterizing individual TAL effectors in rice was established.

71 Rice resistance mechanisms against TAL effectors include polymorphisms that prevent effecto

72 DNA recognition by TAL effectors is mediated by tandem repeats, each 33 to

73 ng region of a transcription activator-like (TAL) effector is used to 'address' a site-specific megan

74 ly compatible with the Golden Gate TALEN and TAL Effector Kit 2.0, a widely used and efficient method

75 The modularity of DNA recognition by TAL effectors makes them important also as tools for gen

76 by the user to work with any TAL effector or TAL effector nuclease architecture.

77 om the FokI-derived zinc-finger nuclease and TAL effector nuclease platforms as the GIY-YIG domain al

78 box to include transcription activator-like (TAL) effector nuclease (TALEN)- and clustered regularly

79 Zinc-finger nucleases (ZFNs) and TAL effector nucleases (TALENs) have been shown to induc

80 domain of FokI restriction enzyme to produce TAL effector nucleases (TALENs) that, in pairs, bind adj

81 meganucleases, zinc-finger nucleases (ZFNs), TAL effector nucleases (TALENs), and CRISPR-associated s

82 , including zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs), have made it possible t

83 generating several cftr mutant alleles using TAL effector nucleases.

84 leases (ZFNs), transcription activator-like (TAL) effector nucleases (TALENs) and clustered regularly

85 Tal-effector nucleases (TALEN) and clustered regularly i

86 TAL-effector nucleases (TALENs) are attractive tools for

87 Tal-effector nucleases (TALENs) are engineered proteins

88 endonucleases, transcription activator-like [TAL] effector nucleases [TALENs], and homing endonucleas

89 A TAL effector-nucleotide binding code that links repeat t

90 developed a suite of web-based tools called TAL Effector-Nucleotide Targeter 2.0 that enables design

91 TAL effectors of plant pathogenic bacteria in the genus

92 We found previously that TAL effectors of the citrus canker pathogen Xanthomonas

93 Transcription activator-like (TAL) effectors of plant pathogenic bacteria contain a mo

94 Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to t

95 ters can be set by the user to work with any TAL effector or TAL effector nuclease architecture.

96 Xanthomonas transcription activator-like (TAL) effectors promote disease in plants by binding to a

97 e Targeter 2.0 that enables design of custom TAL effector repeat arrays for desired targets and predi

98 Transcription activator-like (TAL) effector repeat domains fused to the LSD1 histone d

99 The result indicates that variations in the TAL effector repetitive domains are driven by selection

100 sm for protein-DNA recognition that explains TAL effector specificity, enables target site prediction

101 Our results reveal new modes of action for TAL effectors, suggesting the possibility of yet unrecog

102 AvrHah1 is a transcription activator-like (TAL) effector (TALE) in Xanthomonas gardneri that induce

103 Xoo TAL effectors that promote infection by activating SWEET

104 Incompatibility is associated with major TAL effectors that target the known alternative S genes

105 oduce numerous transcription activator-like (TAL) effectors that increase bacterial virulence by acti

106 rains or by engineering a synthetic designer TAL effector to boost SWEET gene expression.

107 The strong phenotypic similarity between the TAL effector-triggered resistance conferred by Xo1 and t

108 Several Xoc TAL effectors were tested in X11-5A on four rice varieti